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多花菜豆产量性状的灰色关联度分析

西南农业学报 2004年第B5期17卷 249-251页

作者：耿智德王铁军赵银月保丽萍云南省农业科学院粮作所云南昆明650205 云南农业大学农学院昆明650201

应用灰色关联度分析方法,对云南省多花菜豆品种主要性状对其产量的影响进行分析,结果表明:产量与主要性状的关联度大小依次为生育期>单荚粒数>单株产量>单株荚数>单株粒数>百粒重。与产量形成关系密切的主要性状是生育期、单荚粒数、单... 详细信息

应用灰色关联度分析方法,对云南省多花菜豆品种主要性状对其产量的影响进行分析,结果表明:产量与主要性状的关联度大小依次为生育期>单荚粒数>单株产量>单株荚数>单株粒数>百粒重。与产量形成关系密切的主要性状是生育期、单荚粒数、单株产量,并明确了各性状之间的相互关系,为云南省多花菜豆育种和栽培提供理论依据。

关键词：多花菜豆产量性状灰色关联度分析云南品种

Changes of Protective Enzymes and Accumulation of Polyamines Induced by Boron Deficiency in Roots of Rape Seedlings

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Pedosphere 1998年第3期8卷 229-236页

作者： XIAO FENGHUI Department of Biotechnology, yunnan agricultural university, kunming 650201 (china) (Received April 23, 1998 revised June 2, 1998) Department of Biotechnology Yunnan Agricultural University Kunming 650201 China

The effects of boron deficiency on the membrane permeability, the lipid peroxidation of membrane, the activities of the protective enzymes and the accumulation of polyamines in the roots of rape ( Brassica napus L. cv Zhongyou 821) plants were examined using solution culture experiment. Compared to the 20 mmol B m -3 treatment, boron deficient treatment (2 mmol B m -3 ) decreased root dry weight and increased the rate of solute leakage and malondialdehyde (MDA) concentration in the roots of rape. Similar patterns of change were observed in the level of lipid peroxidation (MDA concentration) and the rate of solute leakage under boron deficiency. Results suggested that a significant alteration of membrane composition had occurred under B deficiency. The concentration of putrescine and the ratio of putrescine to spermidine + spermine in the roots of rape increased significantly, and the activities of superoxide dismutase and catalase decreased in the roots of rape during B deficiency. But peroxidase activity in root of B deficient plant was higher than that of control plant.

关键词： boron lipid peroxidation polyamine protective enzymes rape

Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line

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Science china(Life Sciences) 2008年第4期51卷 346-352页

作者： CHEN ShengWei1,2, CHEN PeiDu1 & WANG XiuE1 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing agricultural university, Nanjing 210095, china 2 College of Agronomy and Biotechnology, yunnan agricultural university, kunming 650201, china National Key Laboratory of Crop Genetics Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China College of Agronomy Biotechnology Yunnan Agricultural University Kunming 650201 China

Haynaldia villosa Schur. (syn. Dasypyrum villosum Candargy, 2n=14, VV) has been proved to be an important genetic resource for wheat improvement. The development of translocation with small alien chromosome segments, especially interstitial translocation, will be helpful for better utilization of its useful genes. Up to now, most of the reported Triticum aestivum – H. villosa translocation lines are involved in a whole arm or large alien fragments. In this paper, we report a highly efficient approach for the creation of small chromosome segment translocation lines. Before flowering, the female gametes of wheat-H. villosa 6VS/6AL translocation line were irradiated by 60CO-γ ray at 160 Rad/M dosage rate and three dosages (1600, 1920, 2240 Rad). Anthers were removed from the irradiated florets on the same day and the florets were pollinated with normal fresh pollens of T. aestivum cv. Chinese Spring after 2-3 days. Genomic in situ hybridization (GISH) at mitosis metaphase of root-tip cell of M1 plants was used to detect the chromosome structural changes involving 6VS of H. villosa. Among the 534 M1 plants screened, 97 plants contained small segment chromosome structural changes of 6VS, including 80 interstitial translocation chromosomes, 57 terminal translocation chromosomes and 55 deletion chromosomes. For the 2240 Rad dosage treatment, the inducement frequencies of interstitial translo-cation, terminal translocation and deletion were 21.02%, 14.01%, and 14.65%, respectively, which were much higher than those previously reported. The M2 seeds were obtained by backcrossing of 74 M1 plants involving 146 chromosomes structural changes of 6VS, and it was found that the structural aberrations in the M1 plants could be transmitted to their progenies. Irradiating mature female gametes of whole arm translocation is a new and highly efficient approach for creation of small segment chromosome struc-tural changes, especially for interstitial translocations.

关键词： irradiation mature female gametes small fragment translocation chromosome wheat Haynaldia villosa genomic in situ hybridization

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural Science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. College of Animal Science and Technology, china agricultural university, Beijing 100094, china 2. College of Biological Science, china agricultural university, Beijing 100094, china 3. College of Animal Science and Technology, yunnan agricultural university, kunming 650201, china) College of Animal Science and Technology China Agricultural University Beijing 100094 China College of Biological Science China Agricultural University Beijing 100094 China College of Animal Science and Technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

Influence of Microwave Radiation on Properties and Structure of Fe_2O_3/SO_4^(2-) Solid Superacid Catalyst

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Journal of Materials Science & Technology 1996年第4期12卷 307-311页

作者： Yinan NING (kunming Institute of Precious Metals, kunming 650221, china)Jiaqiang WANG and Pinjie HONG(yunnan university, kunming 650091, china) yunnan UNIV KUNMING 650091PEOPLES R CHINA

The microwave radiation method was introduced to prepare the Fe_2O_3/SO solid *** structure and properties were investigated by means of X-ray diffraction and infrared spectrum analyses as well as measurement of magnetic susceptibility and rate of esterification. The structure of the superacids prepared in microwave field can be crystalline or non-crystalline, the latter has not been reported yet in literatures. Comparing with the traditional superacid, the non-crystalline Fe2O3/SO superacid prepared in microwave field has the highest magnetic susceptibility and catalytic activity. The di-coordination of Fe2O3 and SO and the S=O bi-bond were reinforced by microwave radiation, which is favorable for increasing the acid intensity of the Fe2O3/SO catalyst

关键词： SO Influence of Microwave Radiation on Properties and Structure of Fe2O3/SO4 Solid Superacid Catalyst Fe

The role of MC1R gene in buffalo coat color

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Science china(Life Sciences) 2010年第2期53卷 267-272页

作者： MIAO YongWang1,2,WU GuiSheng3,WANG Lei2,LI DaLin4,TANG ShouKun5,LIANG JianPing2,MAO HuaMing2,LUO HuaiRong3 & ZHANG YaPing1,6 1 Laboratory for Conservation and Utilization of Bio-resource,yunnan university,kunming 650091,china 2 Faculty of Animal Science and Technology,yunnan agricultural university,kunming 650201,china 3 kunming Institute of Botany,Chinese Academy of Sciences,kunming 650204,china 4 yunnan Institute of Buffalo Science and Technology,kunming 650021,china 5 Animal Husbandry and Veterinary Station of Luxi city,Luxi 678400,china 6 State Key Laboratory of Genetic Resources and Evolution,kunming Institute of Zoology,Chinese Academy of Sciences,kunming 650223,china 1. Laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming 650091 China2. Faculty of Animal Science and Technology Yunnan Agricultural University Kunming 650201 China3. Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China4. Yunnan Institute of Buffalo Science and Technology Kunming 650021 China5. Animal Husbandry and Veterinary Station of Luxi city Luxi 678400 China6. State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China

Melanocortin-1 receptor (MC1R) plays a major role in pigmentation in many *** investigate if the MC1R gene is associated with coat color in water buffalo,the coding region of MC1R gene of 216 buffalo samples was sequenced,which included 49 black river buffalo (Murrah and Nili-Ravi),136 swamp buffalo (Dehong,Diandongnan,Dechang,Guizhou,and Xilin) with white and gray body,and 31 hybrid offspring of river buffalo Nili-Ravi (or Murrah) and swamp *** the three variation sites found,SNP684 was synonymous,while SNP310 and SNP384 were nonsynonymous,leading to p.S104G and p.I128M changes,*** Individuals carrying homozygote EBR/EBR were *** genotype and phenotype analysis of the hybrid offspring of black river buffalo and gray swamp buffalo further revealed that the river buffalo type allele EBR or the allele carrying the amino acid p.104S was important for the full function of *** in silico functional analysis showed that the amino acid substitutions p.G104S and p.M128I had significant impact on the function of *** results indicate that the allele EBR or the allele carrying the amino acid p.104S was associated with the black coat color in buffalo.

关键词： Buffalo coat color MC1R

The right dual of left C-rpp semigroups

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Chinese Science Bulletin 1997年第19期42卷 1599-1603页

作者： GUO YuqiDepartment of Mathematics, yunnan university, kunming 650091, china Department of Mathematics Yunnan University Kunming China

SEMIGROUP S is said to be rpp (dually, lpp) if every principal right ideal aS^1(S^1a) of S, re-garded as a right (left)S^1-system, is projective. The semigroup which is both rpp and lpp is said to be abundant. These kinds of generalized regular semigroups have aroused wideattention. The Green’s,relations (~*-Green’s relations introduced by Pastijn) have formed avery useful tool in study on regular (abundant) semigroups. In order to develop the study onrpp semigroups a step further, we introduced the so-called (1)-Green’s,relation which

关键词： rpp semigroups left (right) C-rpp semigroups. (I)-Green’s relations.

A Preliminary Study on the Relationship Between the Indica-Japonica RAPD Differentiation of Parents and Heterosisin Dian Type Hybrid Rice

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agricultural Sciences in china 2002年第12期1卷 1303-1309页

作者： LONG Wen-hong , XU Ming-hui and ZHANG Shu-hua(College of Landscape and Horticulture, yunnan agricultural university, kunming 650201 , P. R. china Institute of Biotechnology , yunnan Academy of agricultural Sciences , kunming 650223 , P. R. china Rice Research Institute, yunnan agricultural university , kunming 650201 , P. R. china) College of Landscape and Horticulture Yunnan Agricultural University Kunming 650201 P. R. China

The relationship between the indica-japonica differentiation of parents and heterosis of hybrid in rice was studied with 10 sterile lines, 10 maintainer lines of the sterile lines, 38 restorer lines of Dian type hybrid rice and 40 hybrids derived from the sterile lines and restorer lines. The results indicated that there was a parabolic correlation between the indica-japonica differentiation of parents detected by RAPD markers and heterosis of hybrid rice. When certain indica-japonica differentiation of parents exists, the percentage of combinations with positve heterosis and the mean heterosis of hybrids were high. But that didn't mean too much difference of parents could lead to stronger hetesosis, that meant overdiffernt parents seldom bring positve het-erotic hybrids. Suitable difference of percent of indica alleles of parents of stronger heterotic hybrid was 12 -16%.

关键词： Dian type hybrid rice Indica-japonica differentiation Heterosis RAPD

3D visualization model and key techniques for digital mine

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中国有色金属学会会刊：英文版 2011年第S3期21卷 748-752页

作者： JIN Bao-xuan1,FANG Yuan-min2,SONG Wei-wei2 1. yunnan Provincial Geomatics Centre, kunming 650034, china 2. Faculty of Land Resource Engineering, kunming university of Science and Technology, kunming 650093, china [a] yunnan Provincial Geomatics Centre Kunming 650034 China [b] Faculty of Land Resource Engineering Kunming University of Science and Technology Kunming 650093 China

Digital mine is the inevitable outcome of the information processing, and is also a complicated system engineering. Firstly, for the 3D visualization application of the digital mine, the ground and underground integrative visualization framework model was proposed based on the mine entity database. So, the visualization problem was availably resolved, as well as the professional analytical ability was improved. Secondly, aiming at the irregularities, non-uniformity, dynamics of mine entities, mix modeling method based on the entity character was put forward, in which 3D expression of mine entities was realized. Lastly, the 3D visualization project for a copper mine was experimentally studied. Satisfactory results were acquired, and the rationality of visualization model and feasibility of 3D modeling were validated.

关键词： digital mine three dimension visualization framework model spatial data modeling