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黄、东海冬季底层鱼类群落结构及多样性变化

水产学报 2004年第1期28卷 29-34页

作者：程济生俞连福农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院东海水产研究所山东青岛 266071 中国水产科学研究院黄海水产研究所山东青岛 266071 上海 200090

根据1991年和2000年冬季在黄海中南部和东海中北部海域进行的底拖网调查资料,对黄、东海底层鱼类群落的资源状况、重要种类的生态优势度与结构、多样性及其空间分布格局等方面的动态变化进行了分析。结果表明,生物量指数明显下降,个体... 详细信息

根据1991年和2000年冬季在黄海中南部和东海中北部海域进行的底拖网调查资料,对黄、东海底层鱼类群落的资源状况、重要种类的生态优势度与结构、多样性及其空间分布格局等方面的动态变化进行了分析。结果表明,生物量指数明显下降,个体小型化使生物资源密度显著上升;重要种类成分发生演替,优势度有较大变化;物种丰富度指数(D)下降极其显著,Shannon-Wiener多样度指数(H′)也有所下降,但不显著。

关键词：底层鱼类群落结构多样性黄东海

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中国对虾杆状病毒一个DNA片段序列测定

海洋学报 2003年第S2期25卷 186-189页

作者：孔杰刘萍石拓相建海中国水产科学研究院黄海水产研究所山东青岛266071 中国科学院海洋研究所山东青岛266071

1　引言杆状病毒引发对虾急性死亡已经成为全球性对虾养殖的问题,在对虾养殖的主要国家和地区均有报道.该病毒引发对虾发病的主要临床表现是在对虾头胸甲有明显的白色斑点,因此有白斑病病毒(whitespotdiseasevirus,WSDV)或白斑综合症病... 详细信息

1　引言杆状病毒引发对虾急性死亡已经成为全球性对虾养殖的问题,在对虾养殖的主要国家和地区均有报道.该病毒引发对虾发病的主要临床表现是在对虾头胸甲有明显的白色斑点,因此有白斑病病毒(whitespotdiseasevirus,WSDV)或白斑综合症病毒(whitespotsyndromevirus,WSSV)之称[1,2].国内研究者曾将引起中国对虾(Penaeuschinenesis)爆发性流行病的杆状病毒命名为皮下及造血组织坏死杆状病毒(hypodermalandhematopoieticnecrosisbaculovirus,HHNBV)[3];而日本研究者对引起日本对虾(Penaeusj

关键词：中国对虾杆状病毒 DNA碱基序列

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卤虫携带白斑综合征病毒的研究

水产学报 2005年第4期29卷 524-528页

作者：邓灯王伟继刘萍孟宪红孔杰中国海洋大学水产学院中国水产科学研究院黄海水产研究所中国水产科学研究院黄海水产研究所山东青岛266003中国水产科学研究院黄海水产研究所山东青岛266071

卤虫是虾、蟹苗种培育的关键动物性饵料,查清其能否携带白斑综合征病毒,对培育健康苗种至关重要。2002年7月至10月,自盐田中取野生卤虫,经暂养后选20尾抱卵雌虫,每尾置1个烧杯中,投喂单胞藻,产卵后取出亲体;20d后,每个烧杯中取5尾子一... 详细信息

卤虫是虾、蟹苗种培育的关键动物性饵料,查清其能否携带白斑综合征病毒,对培育健康苗种至关重要。2002年7月至10月,自盐田中取野生卤虫,经暂养后选20尾抱卵雌虫,每尾置1个烧杯中,投喂单胞藻,产卵后取出亲体;20d后,每个烧杯中取5尾子一代个体,利用巢式体外DNA扩增技术对亲体和子一代成体进行WSSV病毒检测。检测结果显示:卤虫中可扩增出特异性的、与引物设计吻合的DNA片段;DNA测序分析表明,扩增片段的DNA序列与白斑综合征病毒序列一致;野生卤虫携带白斑综合征病毒的阳性率为58%,实验条件下野生卤虫后代的阳性率为43%;同一家系中,亲代与子代携带病毒的情况不完全一致,不能确定白斑综合征病毒可否通过繁殖,在卤虫世代间进行垂直传播。研究结果表明:卤虫很可能是白斑综合征病毒的携带者,苗种培育和养殖期间投喂卤虫可能造成虾、蟹感染白斑综合征病毒,卤虫检疫是培育健康苗种的关键措施。

关键词：白斑综合征病毒卤虫巢式聚合酶链式反应

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鳀人工授精和孵化

水产学报 2002年第2期26卷 175-179页

作者：万瑞景蒙子宁中国水产科学研究院黄海水产研究所厦门大学海洋系山东青岛266071 福建厦门361005

The natural parent fish of Engraulis japonicus was caught with bottom trawler from Haizhou Bay in the yellow sea and the eggs were artificially fertilized. The fertilized eggs began to divide in 40min, to be 16 cell stage in 1h30min, late cleavage stage in 2h50min, blastula stage in 5h, closure of the blastopore in 17h10min, hatching in 37h50min after fertilization, and the process of embryonic development was about 38h at 21.6～ 24.0℃ .

关键词：  授精孵化胚胎发育

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带鱼人工授精和孵化

水产学报 2003年第2期27卷 188-192页

作者：万瑞景蒙子宁农业部海洋渔业资源可持续利用重点开放实验室农业部海洋渔业资源可持续利用重点开放实验室山东青岛　266071 中国水产科学研究院黄海水产研究所山东青岛　266071 厦门大学海洋系福建厦门　361005

The mature parent fish of Trichiurus lepturus was caught with bottom trawl on board R/V 'Beidou' in the Haizhou Bay, yellow sea. The running eggs were artificially fertilized. The fertilized eggs began to divide in 1h30min, to be 16cell stage in 2h55min, late cleavage stage in 4h25min, blastula stage in 10h30min, closure of the blastopore in 25h45min, hatching in 78h45min after insemination, and the process of embryonic development was about 79h at 20.6-22.6℃.

关键词：带鱼授精孵化胚胎发育

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对虾免疫机能研究概况

水产学报 2004年第2期28卷 209-215页

作者：陈国福黄倢宋晓玲上海水产大学生命科学与技术学院中国水产科学研究院黄海水产研究所中国水产科学研究院黄海水产研究所上海　200090 山东青岛　266071

Shrimp farming is an important source of revenue and employment in many developing ***,infectious diseases have adversely affected the profitability of shrimp *** this reason,disease prevention is a priority and shrimp immunology has become a crucial research area of this field. In this paper,the current importance and problems of shrimp-culture were described and the research advances in shrimp immunological defence mechanisms were summarized. The immunological tools are powerful and useful to evaluate the health state of the shrimp. The immunologies of shrimp mainly consist of cellular immunity and humoral immunity. In regard to cellular parameters, they are composed of haemocyte count (THC), differential haemocyte count (DHC) and reactive oxygen intermediates (ROIs). The immunity cells exert their defence functions through phagocytizing, enveloping, etc,and the changes of THC and DHC are related to health state of shrimp. The ROIs generated during post phagocytic event which maybe an important marker to evaluate the immunological capability and phenoloxidase activity have been considered as a potential marker which is relevant to the health of the shrimp too. Concerning humoral parameters, prophenoloxidase (ProPO) and phenoloxidase, antimicrobial peptides and proteins, hemagglutinin and plasma proteins were described. The determining methods of immunity parameters were discussed. The response of shrimp to pathogens such as bacteria, virus, etc. and environmental factors such as DO, pH, etc, were also reviewed. It is well-known that the immune responses induced by immunizing crustacean or shrimp are mainly the non-specific immune responses. The potential of immunological parameters, including the changes of THC and DHC, the production of ROIs, phenoloxidase (PO) activity, antibacterial activity of plasma, and so on, to appraise the healthy state of shrimp were partly discussed. The future directions for the evaluation of the immunological ca

关键词：对虾免疫综述

CLONING,EXPRESSION OF CHLAMYS FARRERI ACUTE VIRAL NECROSIS VIRUS DUTPASE GENE AND THE DETERMINATION OF ITS ENZYMATIC ACTIVITY

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CLONING,EXPRESSION OF CHLAMYS FARRERI ACUTE VIRAL NECROSIS V...

第十八次国际扇贝大会

作者： Zhilei Jia~(1,2),Chongming Wang~2,Weicheng Ren~3,Yantao Liang~2,Peng Qu~(1,2),Yun Li~1 1 Key Lab of Mariculture of the Ministry of Education,Ocean University of china,qingdao 266003,china 2 yellow sea fisheries research institute,chinese academy of fishery sciences,qingdao 266071,china 3 Department of Rheumatology and Inflammation research,University of Gothenburg, Gothenburg,Sweden

Acute virus necrosis virus(AVNV) was reported as one causative agent responsible for summer mass mortality of adult Zhikong scallop(Chlamys farreri),which is widely cultured on northern china *** explore its pathogenesis at the molecular level,we cloned a gene which was predicted to encode AVNV dUTPase based on the genomic sequence of *** AVNV completed by our *** gene encodes a protein of 248aa with a predicted molecular mass of 26.4 *** obtain the *** AVNV Open Reading Frame 074, which supposed encodes AVNV dUTPase,a pair of specific primers was designed based on the genomic sequence of *** *** we amplified the expected DNA by PCR, using the total genomic DNA extracted from infected *** tissues as *** PCR fragments were subcloned into the prokaryotic expression vector pET-32a(+).After that we transformed the recombinant plasmid pET32a-dut into *** BL21(DE3) strain and expressed it under IPTG ***-PAGE analysis showed that the molecular mass of the induced recombinant protein was about 46 *** western-blot and mass spectrometry analysis proved that the expressed protein is the target *** we purified recombinant protein with Co purification column and got the recombinant protein with a purity of more than 90%.The analysis of the enzymatic activity indicated that the recombinant dUTPase could specifically catalyse the hydrolysis of dUTP and the activity of enzyme was enhanced by Mg2+ while inhibited by EDTA.

关键词： dUTP pyrophosphatase Chlamys farreri AVNV prokaryotic expression enzymatic activity

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RAPD技术在中国对虾群体鉴别上的应用

海洋学报 2003年第4期25卷 67-71页

作者：刘振辉孔杰刘萍邓景耀中国海洋大学海洋生命学院山东青岛266003 中国水产科学院黄海水产研究所山东青岛266071

采用RAPD技术对中国对虾(Penaeus chinensis)、中国黄海、渤海沿岸种群(H)朝鲜半岛西海岸种群(K)和即墨健康养殖群体(S)的DNA进行检测,41个随机引、物在3个群体间共筛选到了3个稳定的RAPD标记F-02_(540),OPN-13_(800)和OPN-20_(2200),... 详细信息

采用RAPD技术对中国对虾(Penaeus chinensis)、中国黄海、渤海沿岸种群(H)朝鲜半岛西海岸种群(K)和即墨健康养殖群体(S)的DNA进行检测,41个随机引、物在3个群体间共筛选到了3个稳定的RAPD标记F-02_(540),OPN-13_(800)和OPN-20_(2200),可用于群体间的鉴别,研究将为中国对虾分子标记遗传育种提供依据。

关键词： RAPD 中国对虾群体鉴别养殖标记遗传育种 DNA检测

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脊尾白虾微卫星富集文库的构建与多态性标记的筛选

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水产学报 2011年第12期35卷 1787-1794页

作者：贾舒雯刘萍韩智科李健潘鲁青中国海洋大学水产学院中国水产科学研究院黄海水产研究所

采用人工合成的生物素标记(AG)15探针及磁珠富集法构建了脊尾白虾基因组微卫星富集文库。将脊尾白虾基因组DNA经HaeⅢ酶切后,收集400～1 200 bp片段,连接特定接头,与生物素标记(AG)15探针杂交并捕获含有微卫星的DNA片段,然后连接到pMD1... 详细信息

采用人工合成的生物素标记(AG)15探针及磁珠富集法构建了脊尾白虾基因组微卫星富集文库。将脊尾白虾基因组DNA经HaeⅢ酶切后,收集400～1 200 bp片段,连接特定接头,与生物素标记(AG)15探针杂交并捕获含有微卫星的DNA片段,然后连接到pMD18-T载体中,构建脊尾白虾微卫星富集文库。随机挑取947个克隆,经菌落PCR检验,其中667个为阳性克隆。从阳性克隆中随机选取184个克隆进行测序,有150个克隆含有微卫星序列,共获得199个微卫星序列,其中完美型158个(79.4%),非完美型27个(13.6%),复合型14个(7.0%)。根据微卫星序列,设计了119对微卫星引物,64对扩增出稳定的具有特异性的条带,经30个脊尾白虾个体进行多样性评价后,有26个位点表现出多态性。26个微卫星位点获得的等位基因数从3～16个不等,平均每个位点扩增得到6.5个等位基因。观测杂合度(Ho)、期望杂合度(He)及多态性信息含量值(PIC)的范围分别为0.111～0.929、0.246～0.932、0.231～0.909。本研究开发的微卫星位点将为脊尾白虾种群多样性研究、家系识别和种质鉴定提供有效的工具。

关键词：脊尾白虾富集文库微卫星标记遗传多样性