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Regulation of EGF-induced ERK/MAPK Activation and EGFR Internalization by G Protein-coupled Receptor Kinase 2

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Acta Biochimica et Biophysica Sinica 2005年第8期37卷 525-531页

作者： Jingxia GAO~(1,2*) Jiali LI~1 Lan MA~(1*) ~1 Pharmacology research center,shanghai Medical College.Fudan University,shanghai 200032,china ~2 Department of Biochemistry Medical College,Tongji University,shanghai 200092,china Pharmacology research center Shanghai Medical College Fudan UniversityShanghai 200032 China Department of Biochemistry Medical College Tongji UniversityShanghai 200092 China

G protein-coupled receptor kinases(GRKs)mediate agonist-induced phosphorylation anddesensitization of various G protein-coupled receptors(GPCRs).We investigate the role of GRK2 on epidermalgrowth factor(EGF)receptor signaling,including EGF-induced extracellular signal-regulated kinase andmitogen-activated protein kinase(ERK/MAPK)activation and EGFR *** immunofluorescence experiments show that EGF stimulates GRK2 binding to EGFR complex and GRK2translocating from cytoplasm to the plasma membrane in human embryonic kidney 293 *** assay shows that EGF-induced ERK/MAPK phosphorylation increases 1.9-fold,1.1-fold and 1.5-fold(P20 min,respectively when the cells were transfected with GRK2,suggesting the regulatory role of GRK2 on EGF-induced ERK/MAPK *** cytometry experimentsshow that GRK2 overexpression has no effect on EGF-induced EGFR internalization,however,it increasesagonist-induced G protein-coupled δ opioid receptor internalization by approximately 40% (P2 has a regulatory role in EGF-induced ERK/MAPK activation,and that themechanisms underlying the modulatory role of GRK2 in EGFR and GPCR signaling pathways are somewhatdifferent at least in receptor internalization.

关键词： G protein-coupled receptor kinase receptor tyrosine kinase epidermal growth factor receptor ERK/MAPK internalization

Possible Role of Histone H1 in the Regulation of Furin-dependent Proprotein Processing

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Acta Biochimica et Biophysica Sinica 2007年第3期39卷 173-180页

作者： Jinbo HAN~1 Jianxin GU~2 Chengwu CHI~(1,3*) 1 Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences,shanghai 200031,china 2 Gene research center,Medical center of Fudan University,shanghai 200032,china 3 Institute of Protein research,Tongji University,shanghai 200092,china Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China.

Histone H1 and its C-terminal lysine rich fragments were recently found to be potent inhibitorsof furin,a mammalian proprotein ***,its role in the regulation of furin-dependent proproteinprocessing remains *** we report that histone H1 efficiently blocks furin-dependent pro-yonWillebrand factor（pro-vWF）processing in a dose-dependent *** and immunof-luorescence studies confirmed that histone H1 could interact with furin,and the interaction mainly took placeon the cell *** noted that histone H1 was released from cells undergoing necrosis and apoptosisinduced by *** findings suggested that histone H1 might be involved in extracellular and/or intracellu-lar furin regulation.

关键词： furin histone H1 pro-vWF proprotein processing

cvhA Gene of Streptomyces hygroscopicus 10-22 Encodes a Negative Regulator for Mycelia Development

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 271-280页

作者： Heng-An WANG~(1,2#) Lei QIN~(2#) Ping LU~(1*) Zhi-Xuan PANG~2 Zi-Xin DENG~(3*) Guo-Ping ZHAO~(2*) 1 Biotechnology Institute,School of Agriculture and Biology,shanghai Jiaotong University,shanghai 201101,china 2 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institute for Biological Sciences,Chinese Academy of Sciences,shanghai 200032,china 3 Bio-X Life Science research center,shanghai Jiaotong University,shanghai 200030,china. Biotechnology Institute School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 201101 China Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Chinese Academy of SciencesShanghai 200032 China Bio-X Life Science research center Shanghai Jiaotong UniversityShanghai 200030 China.

A five-gene cluster cvhABCDE was identified from Streptomyces hygroscopicus *** thefirst gene of this cluster,cvhA encoded a putative sensor histidine kinase with a predicted sensor domainconsisting of two trans-membrane segments at the N-terminus and a conserved HATPasecdomain at the *** C-terminus polypeptide of CvhA expressed in Escherichia coli was purified and shown to beautophosphorylated with [γ-32P]ATP in *** phosphoryl group was acid-labile and basic-stable,whichsupported histidine as the phosphorylation *** obvious difference of mycelia development was ob-served between the null mutant of cvhA generated by targeted gene replacement and the wild-type parentalstrain 10-22 grown on solid soya flour medium with 2%-8% glucose or sucrose,but the cvhA mutant couldform much more abundant aerial mycelia and spores than the wild-type strain on solid soya flour mediumsupplemented with 6%-8% mannitol,6%-8% sorbitol,4%-6% mannose,or 4%-6% *** pheno-type was complemented by the cloned wild-type cvhA gene,and no difference was observed for growthcurves of the cvhA mutant and the wild strain in liquid minimal medium with the tested sugars at a concen-tration of 4%,6% and 8%.We thus propose that CvhA is likely a sensor histidine kinase and negativelyregulates the morphological differentiation in a sugar-dependent manner in *** 10-22.

关键词： sensor histidine kinase aerial mycelia sugar metabolism

SHEsis,a powerful software platform for analyses of linkage disequilibrium,haplotype construction,and genetic association at polymorphism loci

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Cell research 2005年第2期15卷 97-98页

作者： Yong Yong SHI1,2, Lin HE2,3,*1Bio-X Life Science research center, shanghai Jiao Tong University, shanghai, china 2Institute for Nutritional Sciences, shanghai Institute of Biological Sciences, Chinese Academy of Sciences, shanghai,china 3NHGG, Bio-X center, shanghai Jiao Tong University, shanghai, china Bio-X Life Science research center Shanghai Jiao Tong University Shanghai China Institute for Nutritional Sciences Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai China NHGG Bio-X Center Shanghai Jiao Tong University Shanghai China

In multiloci-based genetic association studies of complex diseases, a powerful and high efficient tool for analyses oflinkage disequilibrium (LD) between markers, haplotype distributions and many chi-square/p values with a large numberof samples has been sought for long. In order to achieve the goal of obtaining meaningful results directly from raw data,we developed a robust and user-friendly software platform with a series of tools for analysis in association study withhigh efficiency. The platform has been well evaluated by several sets of real data.

关键词： software linkage disequilibrium haplotype analysis genetic association study.

EMMA:An Efficient Massive Mapping Algorithm Using Improved Approximate Mapping Filtering

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 857-864页

作者： Xin ZHANG~1 Zhi-Wei CAO~2 Zhi-Xin LIN~3 Qing-Kang WANG~1* Yi-Xue LI~4 1 Institute of Micro/Nano Science and Technology,shanghai Jiaotong University,shanghai 200030,china 2 shanghai center for Bioinformation Technology,shanghai 200235,china 3 College of Life Science and Technology,shanghai Jiaotong University,shanghai 200030,china 4 Bioinformation center of shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,china Institute of Micro/Nano Science and Technology Shanghai Jiaotong UniversityShanghai 200030 China shanghai center for Bioinformation Technology Shanghai 200235 China College of Life Science and Technology Shanghai Jiaotong UniversityShanghai 200030 China Bioinformation center of shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

Efficient massive mapping algorithm (EMMA),an algorithm on efficiently mapping massivecDNAs onto genomic sequences,has recently been *** process of mapping massive cDNAsonto genomic sequences has been improved using more approximate mapping filtering based on an enhancedsuffix array coupled with a pruned fast hash table,algorithms of block alignment extensions,and *** compared with the classical BLAT software in this field,the computing of EMMA ranges fromtwo to forty-one times faster under similar prediction precisions.

关键词： cDNA mapping maximal exact match enhanced suffix array pruned fast hash table extension algorithm

TNF receptor-associated factor-2 binding site is involved in TNFR75-dependent enhancement of TNFR55-induced cell death

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Cell research 2001年第3期11卷 217-222页

作者： LU FANG, JIA FANG, CHANG QING CHEN shanghai research center of Biotechnology, shanghai Institutes for Biological Sciences the Chinese Academy of Sciences, shanghai 200233, china shanghai research center of Biotechnology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai China

TNF recepter-55 is the main mediator of TNF-induced apoptosis. TNF receptor-75-dependent induction or enhancement of cytotoxicity has been explained by intracellular signaling, 'ligand passing' , or induction of endogenous TNF. To study the function of human TNF receptor-75 (hTR75) and the interaction between human TNF receptor-55 (hTR55) and hTR75 in hTNFa-induced cytotoxicity, HEp-2 cells were transfected with bicistronic expression vector of hTR75 and its deletion mutants genes. hTNFa-induced cytotoxicity was determined by crystal violet colorimetric method. The expression of hTR75 and its deletion mutants in HEp-2 cells was demonstrated by RT-PCR and indirect ELISA. We found that the overexpressed hTR75 could significantly increase the susceptibility of HEp-2 cells to hTNFa which especially required TRAF2 binding site. hTR75 could not only mediate partial hTNFa-induced cytotoxicity independently but also fulfill an accessory role in enhancing or synergizing hTR55-mediated cytotoxicity

关键词： hTNFa hTNF receptors TRAF2 cytotoxcity signal transduction.

Preparation of PFSA/PSf hollow fiber composite membranes with recovered PFSA for the pervaporation separation of EtOH/H_2O

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Science china Chemistry 2010年第1期53卷 273-280页

作者： LANG WanZhong1,2,TONG Wei2,XU ZhenLiang2 & GUO YaJun1 1Department of Chemistry,shanghai Normal University,shanghai 200234,china 2Chemical Engineering research center,East china University of Science and Technology,shanghai 200237,china Department of Chemistry Shanghai Normal University Shanghai China Chemical Engineering research center East China University of Science and Technology Shanghai China

Perfluorosulfonic acid/Polysulfone(PFSA/PSf) hollow fiber composite membranes have been prepared by dip-coating method using PSf ultrafiltration(UF) membrane as substrate with recovered *** composite membranes were applied to the pervaporation separation of 95% ethanol(EtOH)/H2O *** images show that the thickness of the PFSA skin layer of the composite membranes is about 2 μm,much thinner than those of other PFSA composite membranes revealed in the *** of annealing temperature,coating solution concentration and counter-ions of PFSA on the pervaporation performances of the composite membranes were *** total flux decreases and separation factor increases with the increase of annealing *** highest permeation flux of 3230 g m-2 h-1 and a separation factor of 5.4 is obtained for the composite membrane annealed at 80°*** lowest permeation flux of 396 g m-2 h-1 and a separation factor of 27.7 is obtained for the composite membrane annealed at 160°*** permeation performances of the PFSA/PSf composite membrane are evidently influenced by the counter-ions of *** flux sequence of the PFSA/PSf composite membranes with different counter-ions is H+>Li+>Ca2+>Mg2+>Na+>K+>Ba2+>Fe3+>Al3+,and the separation factor sequence is H+2+2+2+Na+>K+.There are very little variations of △Eapp values between the composite membranes with three divalent counterions(Mg2+,Ca2+ and Ba2+),and the △Eapp values of the composite membranes with two trivalent counterions(Fe3+ and Al3+) are relatively high.

关键词： pervaporation hollow fiber composite membrane perfluorslulfonic acid(PFSA) ethanol(EtOH)/H2O mixture

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Two Observed Regions in B Lymphocyte Stimulator Important for Its Biological Activity

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 227-232页

作者： Qiong SHEN~(1,2) Su-Xia LI~1 Fei-Hao FU~1 Qin-Sheng YUAN~1* Yi GONG~2* 1 State Key Laboratory of Bioreactor Engineering,East china University of Science and Technology,shanghai 200237,china 2 research center of Biotechnology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200233,china State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237 China research center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200233 China

B lymphocyte stimulator （BLyS）,a member of the tumor necrosis factor superfamily ofligands,is a crucial survival factor for B *** successfully constructed seven mutants of the functionalsoluble fragment of human BLyS （named cBLyS,amino acid 134-285）,including three deletion mutants andfour site-directed *** the mutant proteins were expressed in Escherichia coli and purified by Ni-NTA affinity *** biological activities of these mutants were assessed by the ligand-recep-tor binding assay,B cell proliferation assay and immune effect response in *** results indicated thatfour residues,H218,F220,T228and L229,are indispensable for the biological activity of cBLyS,whereas tworegions,amino acid 134-148 and amino acid 271-285,are related to the biological activity of *** of deletion of amino acid 134-148 leads to a complete defection in raising the antigen-specific *** deletion of amino acid 271-285 reduces the effectiveness compared with the native *** that the region of amino acid 134-148 is indispensable for cBLyS to function normally.

关键词： B lymphocyte stimulator (BLyS) deletion mutant biological activity

Effect of heat-treatment on the surface properties of gallium phosphide nanosolids by Raman spectroscopy

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Rare Metals 2006年第3期25卷 253-259页

作者： ZHANG Zhaochun YUE Longyi GUO Jingkang School of Materials Science and Technology Shanghai University Shanghai 200072 China Bioinformatics research center Shanghai University Shanghai 200072 China

Raman spectra of gallium phosphide （GAP） nanosolids （unheated and heat-treated at 598 and 723 K, respectively） were investigated. It was observed that both the longitudinal optical mode （LO） and the transverse optical mode （TO） displayed an asymmetry on the low-wavenumber side. The scattering bands were fitted to a sum of four Lorentzians which were assigned to the LO mode, surface phonon mode, TO mode, and a combination of Ga-O-P symmetric bending and sum band formed from the X-point TA ＋ LA phonons, respectively. Analysis of the characteristic of surface phonon mode revealed that the surface phonon peak of the GaP nanosolids could be confirmed. In the infrared spectrum of the GaP nanoparticles, we observed the bands on account of symmetric stretching and bending of PO2, as well as stretching of Ga-O The Raman scattering intensity arising from the Ga-O-P linkages increased as increasing the heat-treatment temperature.

关键词： semiconductor materials nanosolids Raman spectrum gallium phosphide

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