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CMV-Pf-CP基因表达载体的构建及转化西番莲(Passiflora edulis)的研究

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西南农业学报 2001年第4期14卷 10-13页

作者：刘志昕魏源文中国热带农业科学院热带作物生物技术国家重点实验室海南海口571101 广西农业科学院生物技术研究所广西南宁530007

以黄瓜花叶病毒 (Cucumbermosaicvirus ,CMV )西番莲分离物的外壳蛋白基因(CMV Pf CP)为目的基因 ,插入 pBI12 1质粒 35S启动子后 ,构建了植物表达载体 pBIC ;以子叶和胚轴为外植体 ,初步建立了西番莲转基因体系 ,采用土壤根癌农杆菌 (A... 详细信息

以黄瓜花叶病毒 (Cucumbermosaicvirus ,CMV )西番莲分离物的外壳蛋白基因(CMV Pf CP)为目的基因 ,插入 pBI12 1质粒 35S启动子后 ,构建了植物表达载体 pBIC ;以子叶和胚轴为外植体 ,初步建立了西番莲转基因体系 ,采用土壤根癌农杆菌 (Agrobacteriumtumefaciens)介导法进行西番莲转化研究 ,获得了抗卡那霉素的转基因再生植株。通过对所获得的再生植株进行PCR扩增检测 ,结果表明CMV -Pf -CP基因已导入西番莲植株中。

关键词：西番莲黄瓜花叶病毒外壳蛋白基因农村菌介导转基因

Land Use and Soil Organic Carbon in china’s Village Landscapes

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Pedosphere 2010年第1期20卷 1-14页

作者： JIAO Jia-Guo YANG Lin-Zhang WU Jun-Xi WANG Hong-Qing LI Hui-Xin E. C. ELLIS Department of Resources and Environmental sciences Nanjing Agricultural University Nanjing210095 （China） institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Department of Agronomy and Agroecology China Agricultural University Beijing 100094 （China） Department of Geography ＆ Environmental Systems University of Maryland Baltimore County Baltimore 21250 （USA） Center for Louisiana Inland Water Studies University of Louisiana at Lafayette Lafayette Louisiana （USA）

Village landscapes, which integrate small-scMe agriculture with housing, forestry, and a host of other land use practices, cover more than 2 million square kilometers across china. Village lands tend to be managed at very fine spatial scales （≤ 30 m）, with managers both adapting their practices to existing variation in soils and terrain （e.g., fertile plains vs. infertile slopes） and also altering soil fertility and even terrain by terracing, irrigation, fertilizing, and other land use practices. Relationships between fine-scale land management patterns and soil organic carbon （SOC） in the top 30 cm of village soils were studied by sampling soils within fine-scale landscape features using a regionally weighted landscape sampling design across five environmentally distinct sites in china. SOC stocks across china＇s village regions （5 Pg C in the top 30 cm of 2 ×10^6 km^2） represent roughly 4% of the total SOC stocks in global croplands. Although macroclimate varied from temperate to tropical in this study, SOC density did not vary significantly with climate, though it was negatively correlated with regional mean elevation. The highest SOC densities within landscapes were found in agricultural lands, especially paddy, the lowest SOC densities were found in nonproductive lands, and forest lands tended toward moderate SOC densities. Bue to the high SOC densities of agricultural lands and their predominance in village landscapes, most village SOC was found in agricultural land, except in the tropical hilly region, where forestry accounted for about 45% of the SOC stocks. A surprisingly large portion of village SOC was associated with built structures and with the disturbed lands surrounding these structures, ranging from 〉 18% in the North china Plain to about 9% in the tropical hilly region. These results confirmed that local land use practices, combined with local and regional variation in terrain, were associated with most of the SOC variation within and across china＇s

关键词： agriculture land cover change land use change soil carbon sequestration spatial heterogeneity

STUDY ON SUSTAINABLE CONTROL OF ROOT-KNOT NEMATODE DISEASE ON SEASON-OFF VEGETABLES IN HAINAN ISLAND

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STUDY ON SUSTAINABLE CONTROL OF ROOT-KNOT NEMATODE DISEASE O...

The ⅩⅤth International Plant Protection Congress

作者： CHEN Mian-cai XIAO Tong-bin XIE Sheng-hua and WU Feng-zhi institute of Plant Protection Hainan academy of agricultural sciences haikou 571100 china

First Report of Bacterial Leaf Spot of Mango Caused by a Xanthomonas arboricola Pathovar in china

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First Report of Bacterial Leaf Spot of Mango Caused by a Xan...

中国植物病理学会2011年学术年会

作者： Y.X.Qi~(1,2,3),J.J.Pu~(1,2,3),X.Zhang~(1,2,3),Y.Lu~(1,2,3), H.Zhang~1,Y.C.Lv~1,H.Q.Zhang~(1,2,3) and Y.X.Xie~(1,2,3) (1.Environment and Plant Protection institute,chinese academy of tropical agricultural sciences,Danzhou,571737 Hainan,china 2.Key Laboratory of Monitoring and Control of tropical agricultural and Forest Invasive Alien Pests,Ministry of Agriculture,Danzhou,571737 Hainan,china)

＜正＞A disease of mango（Mangifera indica）observed in 2009～2010 in Hainan and Guangxi provinces（china） is characterized by small water-soaked spots on the leaves turning with age into angular,sunken,deep-brown to black necrotic lesions with a yellow halo,whose necrotic tissue may fall,resulting in a "shot-hole" condition of the *** symptoms differ from those of mango bacterial black spot caused by *** *** （Xcm）.Purified single bacterial colonies appeared yellow,slimy and convex on YDCA

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Identification of HbNIN2 as the key invertase responsible for sucrose catabolism in rubber-producing laticifers,a rate-limiting step determining rubber productivity

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Identification of HbNIN2 as the key invertase responsible fo...

第十三届全国植物基因组学大会

作者： Chaorong Tang~(1,*) Shujin Liu~(1,2,#),Jixian Lan~(1,2,#),Binghui Zhou~(1,2),Yunxia Qin~1,Yihua Zhou~3, Jianghua Yang~1,Yuekun Wang~1,Jiyan Qi~1 1 Key Lab of Rubber Biology,Ministry of Agriculture & Rubber Research institute,chinese academy of tropical agricultural sciences,Danzhou,Hainan 571737,china. 2 College of Agronomy,Hainan University,Danzhou,Hainan 571737,china. 3 National Center for Plant Gene Research,institute of Genetics and Developmental Biology, chinese academy of sciences,Beijing 100101,china

The role of neutral/alkaline invertases(NA-lnvs) in determining the sink strength of laticifers and final rubber productivity has been well described in Hevea brasiliensis(Hevea hereafter). However,nothing was known about the relevant gene(s) encoded such NA-lnvs in *** this work,we cloned and functionally characterized the cDNA sequences that code for three NA-lnvs expressed in the *** three cDNAs,designated HbNIN1,HbNIN2 and HbNIN3,

关键词：

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麻竹试管苗的移栽和生长

上海农业学报 1996年第4期12卷 66-68页

作者：陈汉洲张桂和华南热带作物学院农学部中国热带农业科学院热带园艺研究所

麻竹试管苗；经练苗结果表明，以混合土为培养基质，５０％荫蔽度；每３ｄ淋水１次，每７ｄ喷１次０．１％尿素水肥．成活率最高，生长较快；定植大田生长半年后．疏伐老苗，每丛保留３条新壮竹，可提早出笋．

关键词：麻竹试管苗练苗成活率

Isolation and characterization of eight polymorphic microsatellite loci for Brontispa longissima(Gestro)(Coleoptera:Hispidae)

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Isolation and characterization of eight polymorphic microsat...

第三届全国生物入侵大会

作者： C.L.Ma~1,H.L.Wu~1,H.Y.Hu~1,X.Wu~1,G.C.Ma~2,Y.G.Fu~2 and Z.Q.Peng~2 1.Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, College of Life sciences,Anhui Normal University,Wuhu,Anhui,241000,china 2.Key Laboratory of Monitoring and Control of tropical agricultural and Forest Invasive Alien Pests, Ministry of Agriculture, Environment and Plant Protection institute,chinese academy of tropical agricultural sciences, Danzhou,Hainan,571737,china

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia,and was first discovered on Hainan Island in June *** the economic risk associated with the putative stable presence of this pest,the genetic aspects of its invasion process have remained relatively *** microsatellite markers,we can investigate the population structure,genetic variability and pattern of invasion in different geographical *** methodology was based on a modified biotin-capture *** the current study,eight polymorphic microsatellite loci were isolated and characterized for the *** allelic number per locus varied from 2 to 3(N=30).The expected and observed heterozygosities of the eight loci ranged from 0.042 to 0.509 and from 0.042 to 0.963,*** the polymorphisms were not very high in the population,the microsatellite loci isolated in this study will be useful for investigating the genetic diversity and route of transmission in *** populations.

关键词： Beetle Brontispa longissima Microsatellite markers Polymorphism

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SOTER支持下海南土壤侵蚀量估算

水土保持学报 2003年第6期17卷 51-54页

作者：余炜敏漆智平西南农业大学资源与环境学院重庆400716 中国热带农业科学院海南儋州571737

在已建立的SOTER数据库基础上,利用SWEAP模型运算可知:海南土壤侵蚀主要发生在丘陵和陡坡地;海南土壤侵蚀量的空间分布与地形构造一致,中部山区(保亭)土壤侵蚀量较大,北部(琼山)沿海平原区侵蚀量较小;坡度为8％～30％的丘陵地区,采用梯... 详细信息

在已建立的SOTER数据库基础上,利用SWEAP模型运算可知:海南土壤侵蚀主要发生在丘陵和陡坡地;海南土壤侵蚀量的空间分布与地形构造一致,中部山区(保亭)土壤侵蚀量较大,北部(琼山)沿海平原区侵蚀量较小;坡度为8％～30％的丘陵地区,采用梯化或等高种植的耕作措施可以大幅度降低土壤侵蚀量。研究表明,在SOTER数据库的支持下,SWEAP用于海南土壤侵蚀量的估算结果可靠程度较高。

关键词： SOTER 海南土壤侵蚀量估算

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大青山灵芝的化学成分研究

中草药 2017年第3期48卷 437-442页

作者：兰宁宁马青云黄圣卓孔凡栋杨宁宁戴好富吴友根赵友兴海南大学园艺园林学院海南海口570228 中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室海南海口571101

目的研究大青山灵芝Ganoderma daiqingshanense的化学成分。方法采用硅胶柱色谱和Sephadex LH-20凝胶柱色谱等技术进行分离纯化,运用各种波谱方法对化合物进行结构鉴定,并测定部分化合物对乙酰胆碱酯酶(Ach E)的抑制活性。结果从大青山... 详细信息

目的研究大青山灵芝Ganoderma daiqingshanense的化学成分。方法采用硅胶柱色谱和Sephadex LH-20凝胶柱色谱等技术进行分离纯化,运用各种波谱方法对化合物进行结构鉴定,并测定部分化合物对乙酰胆碱酯酶(Ach E)的抑制活性。结果从大青山灵芝中分离鉴定了12个化合物,经鉴定为3β,21β-山芝烯二醇-3-乙酰基(1)、3β,21α-山芝烯二醇-3-乙酰基(2)、3-O-乙酰基千层塔三醇(3)、20(29)-羽扇烯-3-醇(4)、赤芝醛A(5)、灵芝酸Y(6)、麦角甾-7,22E-二烯-3-酮(7)、麦角甾-4,6,8(14),22E-四烯-3-酮(8)、麦角甾-7,22E-二烯-3β-醇(9)、5α,8α-过氧麦角甾-6,22E-二烯-3β-醇(10)、甲龙胆酸(11)、大戟因子L3(12)。化合物5、11和12对乙酰胆碱酯酶的抑制率分别为17.70%、22.89%和21.22%。结论所有化合物均为首次从大青山灵芝中分离得到,其中serratene型三萜(化合物1~3)为首次从灵芝科真菌中发现,化合物5、11和12对Ach E具有一定的抑制活性。

关键词：大青山灵芝灵芝科 3β,21β-山芝烯二醇-3-乙酰基 3β,21α-山芝烯二醇-3-乙酰基 3-O-乙酰基千层塔三醇乙酰胆碱酯酶抑制活性