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Cloning of artemisinin biosynthetic cDNAs and novel ESTs and quantification of low temperature-induced gene overexpression

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Science china(Life Sciences) 2008年第3期51卷 232-244页

作者： ZENG QingPing1, ZHAO Chang1, YIN LuLu1, YANG RuiYi1, ZENG XiaoMei1, HUANG Ying2, FENG LiLing3 & YANG XueQin3 1 Laboratory of Biotechnology, Tropical Medicine Institute, guangzhou University of chinese Medicine, guangzhou 510405, china 2 guangzhou general hospital, guangzhou military area command of chinese pla, guangzhou 510010, china 3 Center of Artemisinin Research, guangzhou University of chinese Medicine, guangzhou 510405, china 1. Laboratory of Biotechnology Tropical Medicine Institute Guangzhou University of Chinese Medicine Guangzhou 510405 China 2. guangzhou general hospital Guangzhou Military Area Command of Chinese PLA Guangzhou 510010 China 3. Center of Artemisinin Research Guangzhou University of Chinese Medicine Guangzhou 510405 China

To isolate and verify novel genes from qinghao (Artemisia annua) based on the development-specific and environment-induced transcriptomics, leaves have been harvested from the flowering A. annua plants and exposed to low temperature for isolation of total RNAs and cloning of full-length cDNAs and cDNA fragments, or expressed sequence tags (ESTs). After being sequenced and browsed for homol- ogy, these sequences have been submitted to GenBank. Among the accessed 75 sequences, 4 full-length cDNAs are highly homologous to the known A. annua genes, but 71 ESTs are absent in the sequence records of A. annua genes, in which 34 sequences are homologous to other plant genes, including 24 identified protein-coding sequences and 10 unidentified protein-coding sequences, while other 37 sequences are not present in the sequence records of any plant genes, representing the first cloned plant genes. In order to investigate the responsive patterns of A. annua genes to extreme envi- ronmental stresses, especially low temperature, the expression levels of 3 critical qinhaosu (artemisi- nin) biosynthetic genes, ADS, CYP71AV1 and CPR, have been measured in pre- and post-chilling A. annua seedlings cultured in vitro by semi-quantitative PCR (SQ-PCR). Consequently, ADS and CYP71AV1 genes are strongly induced by chilling, but CPR gene is not significantly affected by such treatment. Furthermore, induction of these genes by chilling can be potently suppressed by Ca2+ channel inhibitor LaCl3 or Ca2+ chelator EGTA, suggesting a putative involvement of Ca2+-CaM signal transduction pathway in chilling-induced overexpression of ADS and CYP71AV1 genes. The real-time fluorescent quantitative PCR (RFQ-PCR) assay of A. annua seedlings exposed to chilling has shown that the expression level of CaM gene is up-regulated for more than 2.5 folds, thereby confirming our above inference on the relevance of Ca2+-CaM-mediated signal transduction to chilling-induced gene overexpression. Finally, 7 newly iso

关键词： Artemisia annua expressed sequence tag (EST) abiotic stress signal transduction fluorescent quantification

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Summary of the 11th Conference on Magnetic Confined Fusion Theory and Simulation

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plasma Science and Technology 2024年第10期26卷 1-7页

作者： guangzhou HAO Jianqiang XU Youwen SUN Zhibin GUO Organizing Committee of the 11th Conference on Magnetic Confined Fusion Theory and Simulation Southwestern Institute of Physics Chengdu 610041People’s Republic of China Institute of plasma Physics Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031People’s Republic of China State Key Laboratory of Nuclear Physics and Technology Fusion Simulation CenterSchool of PhysicsPeking UniversityBeijing 100871People’s Republic of China 不详

This conference report summarizes recent progress in plasma theory and simulation that was presented in contributed papers and discussions at the 11th Conference on Magnetic Confined Fusion Theory and Simulation(CMCFTS)held in Chengdu,china,27–30 October,*** in various fields has been *** example,results on zonal flow generation by mode coupling,simulations of the key physics of divertor detachment,energetic particle effects on magnetohydrodynamic(MHD)modes in addition to ion-and electron-scale turbulence,physics of edge coherent modes and edge-localized modes,and the optimization of ion heating schemes as well as confinement scenarios using advanced integrated modeling are presented at the *** this conference,the scientific research groups were organized into six categories:(a)edge and divertor physics;(b)impurity,heating,and current drive;(c)energetic particle physics;(d)turbulent transport;(e)MHD instability;and(f)integrated modeling and code development.A summary of the highlighted progress in these working groups is presented.

关键词： magnetic confined fusion(MCF) theory and simulation modeling tokamak

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Changes in muscular strength and electromyogram in rats with muscular disuse atrophy following electrical stimulation

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Neural Regeneration Research 2006年第9期1卷 809-812页

作者： Xiaoyu Lü1, Xuanming Hao2 1Department of Rehabilitation and Physiotherapy, guangzhou general hospital of guangzhou military area command of chinese pla, guangzhou 510010, Guangdong Province, china 2College of Sports Science, South china Normal University, guangzhou 510631, Guangdong Province, china Department of Rehabilitation and Physiotherapy Guangzhou General Hospital Guangzhou Military Area Command Guangzhou 510010 Guangdong Province China College of Sports Science South China Normal University Guangzhou 510631 Guangdong Province China

BACKGROUND: Atrophy of skeletal muscle is found under the condition of muscular disuse or in the process of fixation. It is affected by fixation, and electromyogram (EMG) discharge and muscular strength levels will be significantly decreased with accelerating tendency. Electrical stimulation (ES) therapy can release the velocity of muscular disuse atrophy effectively, so it is an effective method for preventing and treating muscular disuse atrophy and accelerating rehabilitation velocity following removal of fixation. OBJECTIVE: To observe the effect of ES therapy at different time points following the fixation of rat models of muscular disuse atrophy on muscular strength and EMG of quadriceps femoris. DESIGN: A randomized and controlled animal experiment. SETTING: Department of Rehabilitation and Physiotherapy, guangzhou general hospital of guangzhou military area command of chinese pla; College of Sports Science, South china Normal University. MATERIALS: Male SD rats, of clean grade, aged 4 months old, weighing (230±10) g, were provided by the Animal Experimental Center of Sun Yat-sen University. EMG measurement and analysis system (NEC Company, Japan) and four-channel recorder (NEC Company, Japan) were used in this experiment. METHODS: This experiment was carried out in the Laboratory of Human Sports Science, South china Normal University between September 2003 and March 2004. Totally 125 successful SD rat models of muscular disuse atrophy were randomly divided into 5 groups with 25 rats in each by a lot: normal control group, in which, the rats were untouched; ES 24 hours, 1, 2 and 3 weeks groups: the knees of rats in these four groups were fixed. Rats in four groups underwent ES therapy at 24 hours, 1, 2 and 3 weeks after fixation. T90-Ⅱ computer ES muscular strength training instrument was used in ES therapy every other day. The instrument was set as square wave, 5 mA current intensity and 10 minutes a day. Muscular strength of quadriceps femoris and data of E

关键词： electrical stimulation disuse atrophy velocity of strength training animal experiment skeletal muscle used in strength of SD rat

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中国死亡独生子女母亲总量及变动趋势再研究

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人口与经济 2024年第2期 29-43页

作者：刘旭阳王广州首都师范大学管理学院北京100048 中国社会科学院人口与劳动经济研究所北京100006

伴随我国生育水平降低,城镇地区独生子女家庭逐渐成为主流,面临较大的独生子女死亡风险。“七普”数据的公布为探究我国死亡独生子女母亲规模提供了更全面的数据资料,基于“四普”至“七普”数据,汇总不同子女数量的妇女规模,利用年龄... 详细信息

伴随我国生育水平降低,城镇地区独生子女家庭逐渐成为主流,面临较大的独生子女死亡风险。“七普”数据的公布为探究我国死亡独生子女母亲规模提供了更全面的数据资料,基于“四普”至“七普”数据,汇总不同子女数量的妇女规模,利用年龄移算的方法测算得到2020年已经退出育龄期的死亡独生子女母亲规模。结果显示,当前50岁及以上“曾生子女无子女”妇女规模约为288.8万人,其中50—59岁死亡独生子女母亲规模达到191.9万人,占当前死亡独生子女母亲总量的66.4%;“六普”数据中死亡独生子女母亲规模与“七普”数据缺乏一致性,基于“六普”数据到“七普”数据的打靶预测结果表明,“七普”数据可能高估当前死亡独生子女母亲规模;随着生育水平的下降、生育进度的推迟,未来妇女独生子女死亡风险提高,死亡独生子女母亲规模快速增长趋势将保持稳定。基于此,建议一方面加速构建生育支持体系,避免长期生育率低迷问题;另一方面也要进一步完善计划生育特殊家庭扶助制度,多部门协调扩大独生子女父母的养老扶助资源。

关键词：失独曾生子女独生子女第七次全国人口普查

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Magnetic properties of Jiaxian red clay sequences from northern chinese Loess plateau and its paleoclimatic significance

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Science china Earth Sciences 2005年第8期48卷 1234-1245页

作者： QIANG Xiaoke, AN Zhisheng, LI Huamei, CHANG Hong & SONG Yougui 1. SKLLQG, Institute of Earth Environment, chinese Academy of Sciences, Xi’an 710075, china 2. guangzhou Institute of Geochemistry, chinese Academy of Sciences, guangzhou 510640, china 1. chinese Academy of Sciences SKLLQG Institute of Earth Environment 710075 Xi’an China 2. chinese Academy of Sciences Guangzhou Institute of Geochemistry 510640 Guangzhou China

Detailed rock magnetism investigation of the Jiaxian red clay sequences indicated that there are common magnetic mineralogy properties in red clay and loess-paleosol sequences from the chinese Loess plateau as well as the marked properties of themselves, magnetic min-erals mainly with magnetite, maghemite, hematite, and possibly limonite/goethite contributing to the magnetic behavior. Meanwhile, it is found that the strong paleosol in red clay sequences has a lower coercivity and higher content of ultra-fine ferrimagnetic grains than that of the weak pa-leosol, which is similar to loess and paleosol in upper Wucheng Loess sequences, and indicates that humid conditions and relatively strong pedogenesis play a significant role in the increase of ultra-fine magnetic minerals and transformation of the magnetic minerals. This suggests that, like Quaternary loess-paleosols, the change of characteristics of paleoclimatic conditions of the late Tertiary red clay deposits is fluctuant. In addition, the results of magnetic hysteresis properties show that the applied saturated field for samples from the Jiaxian red clay sequences is higher than that of the samples from eastern and southern chinese Loess plateau. It is obviously shown that there exist more hard magnetic minerals and relatively weak biochemical processes in the Jiaxian red clay sequences on northern chinese Loess plateau. We conclude that the paleocli-matic environment is different between northern and eastern/southern chinese Loess plateau, and it should be more arid in northern chinese Loess plateau.

关键词： northern chinese Loess plateau, red clay, magnetic property, paleoclimatic significance.

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益生菌对牛奶蛋白过敏婴幼儿肠道菌群的影响

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中国微生态学杂志 2024年第6期36卷 677-681,687页

作者：姜凯王广州王洁翡郑州市妇幼保健院儿科河南450003 郑州市妇幼保健院新生儿科河南450003 郑州市妇幼保健院急诊科河南450003

目的探讨益生菌对牛奶蛋白过敏(CMPA)婴幼儿肠道菌群丰度的影响及其干预效果。方法选取2022年3月至2023年10月在郑州市妇幼保健院就诊的CMPA患儿60例作为试验组,并选取同期于本院体检的健康婴幼儿30例作为对照组,以随机数字表法将试验... 详细信息

目的探讨益生菌对牛奶蛋白过敏(CMPA)婴幼儿肠道菌群丰度的影响及其干预效果。方法选取2022年3月至2023年10月在郑州市妇幼保健院就诊的CMPA患儿60例作为试验组,并选取同期于本院体检的健康婴幼儿30例作为对照组,以随机数字表法将试验组分为试验1组和试验2组,每组30例。试验1组采用回避膳食干预,试验2组在回避膳食干预的基础上联合益生菌干预。对比干预前试验组和对照组患儿的肠道菌群丰度,并比较干预前后试验1组和试验2组肠道菌群丰度、临床疗效、临床症状(全身症状、皮肤症状、消化道症状和呼吸道症状评分)及血液嗜酸性粒细胞数量(EOS)、免疫球蛋白E(IgE)和皮肤指数(SI)水平。结果与对照组比较,干预前试验组患儿肠道双歧杆菌和乳杆菌丰度显著降低,而大肠埃希菌和肠球菌丰度显著升高(t=5.671、12.201、13.097、25.584,均P2组患儿治疗的总有效率为80.00%,显著高于试验1组的53.33%(χ^(2)=4.800,P2.163、3.036、6.591、11.350、6.970、7.044、7.484、7.153、2.258、4.071、3.195,试验2组:t=4.790、5.454、9.187、14.206、8.841、8.820、8.753、8.690、4.141、7.554、5.537,均P2组中上述指标变化幅度大于试验1组(t=2.197、2.512、4.440、2.788、3.509、2.200、3.270、3.607、2.168、3.576、3.003,均P<0.05)。结论CMPA婴幼儿肠道菌群以双歧杆菌、乳杆菌丰度降低和大肠埃希菌、肠球菌丰度升高为主要特征,使用益生菌干预可有效改善患儿的肠道菌群,提高治疗效果。

关键词：牛奶蛋白过敏肠道菌群益生菌

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STUDIES ON REVERSE OSMOSIS SEPARATION OF AQUEOUS ORGANIC SOLUTIONS BY PAA/PSF COMPOSITE MEMBRANE

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chinese Journal of Polymer Science 2000年第2期18卷 115-122页

作者： Jun Fang De-min Jia Ji-cai Huang Qun-hui Guo Feng-lian Wu South-china University of Technology, guangzhou 510641, china guangzhou Institute of Chemistry, chinese Academy of Sciences, guangzhou 510650, china S china Univ Technol Guangzhou 510641 Peoples R China chinese Acad Sci Guangzhou Inst Chem Guangzhou 510650 Peoples R China

The reverse osmosis (RO) separation of aqueous organic solutions, such as alcohols, amines, aldehydes, acids, ketones, and esters etc., by PAA (polyacrylic acid)/PSF (polysulfone) composite membrane has been studied. It was found that the separation results for aliphatic alcohols, amines and aldehydes are satisfactory, the solute rejection (R-a) and the volume fluxes of solutions (J(V)) for 1000 ppm ethanol, ethylamine and ethyl aldehyde are 66.2%, 61.0%, 84.0% and 0.90 x 10(-6), 0.35 x 10(-6), 0.40 X 10(-6) m(3)/m(2) . s, respectively, at 5.0 MPa and 30 degrees C. R-a increased with increasing molecular weights of alcohols, amines and aldehydes, and the R-a for n-amyl alcohol, n-butylamine and n-butyl aldehyde reached 94.3%, 88.6% and 96.0%, respectively. Satisfactory separation results (R-a > 70%) for ketones, esters, phenols and polyols have been obtained with the PAA/PSF composite membrane. The effect of operating pressure on the properties of reverse osmosis has also been investigated. Analysis of experimental data with Spiegler-Kedem's transport model has been carried out and the membrane constants such as reflection coefficient sigma, solute and hydraulic permeabilities omega and L-p for several organic solutes have been obtained.

关键词： PAA/PSF composite membrane reverse osmosis separation of aqueous organic solution

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Relation between texture and chemical composition of peridotite xenoliths and its implications: An example from Wangqing, Jilin Province

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chinese Science Bulletin 1998年第10期43卷 837-840页

作者： XU Yigang guangzhou Institute of Geochemistry, chinese Academy of Sciences, guangzhou 510640, china guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China

Spinel peridotite xenoliths from Wangqing, NE china exhibit correlative variations in texture, temperature, geochemistry and radiogenic isotopes. This suggests that the Wangqing xenoliths may come from a mantle diapir. It is possible that the fertile and undeformed lherzolites were situated in the interior of the diapir, whereas the refractory and deformed harzburgites and clinopyroxene_poor lherzolites were within the diapiric flanks.

关键词： peridotite xenoliths texture geochemistry Wangqing.

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Nitric Oxide Emission Following Wetting of Dry Soils in Subtropical Humid Forests

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Pedosphere 2009年第6期19卷 692-699页

作者： LI De-Jun and WANG Xin-Ming 2 State Key Laboratory of Organic Geochemistry, guangzhou Institute of Geochemistry, chinese Academy of Sciences, guangzhou 510640 (china) [a]State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 (China)

Information about soil nitric oxide （NO） emissions from subtropical forests is quite limited, and even less is known about the pulse emission of NO when wetting soils after a long period of dryness. In this study, we measured NO fluxes following wetting of dry soft in a broadleaf forest and a pine forest in subtropical china. Large pulses of NO fluxes were observed after soil wetting in both forests. NO fluxes increased significantly within 0.5 h following wetting in both forests and reached peak 1 and 4 h after soil wetting in the pine forest and the broadleaf forest, respectively. In the broadleaf forest, averaged peak flux of NO pulses was 157 ng N m^-2 s^-1, which was 8 times the flux value before wetting, and in the pine forest, the averaged peak flux was 135 ng N m-2 s 1, which was 15.5 times the flux value before wetting. The total pulses-induced NO emissions during the dry season were roughly estimated to be 29.4 mg N m^-2 in the broadleaf forest and 22.2 mg N m^-2 in the pine forest or made up a proportion of 4.6% of the annual NO emission in the broadleaf forest and 5.3% in the pine forest.

关键词： dry season flux. NO. pulse subtropical china

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血管内机械再通术联合重组组织型纤溶酶原激活剂静脉溶栓治疗急性脑梗死的临床研究

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中国实用医刊 2024年第1期51卷 17-21页

作者：于广周驻马店市中心医院神经内科驻马店463000

目的探讨血管内机械再通术联合重组组织型纤溶酶原激活剂静脉溶栓治疗急性脑梗死的临床效果。方法随机对照研究。抽取2020年10月至2022年5月驻马店市中心医院收治的急性脑梗死患者108例,采用照随机数字表法将其分为对照组和观察组,每组5... 详细信息

目的探讨血管内机械再通术联合重组组织型纤溶酶原激活剂静脉溶栓治疗急性脑梗死的临床效果。方法随机对照研究。抽取2020年10月至2022年5月驻马店市中心医院收治的急性脑梗死患者108例,采用照随机数字表法将其分为对照组和观察组,每组54例。对照组在常规治疗基础上给予重组组织型纤溶酶原激活剂静脉溶栓治疗,观察组患者在常规治疗基础上给予血管内机械再通术联合重组组织型纤溶酶原激活剂静脉溶栓治疗。比较两组患者的临床疗效,比较两组治疗前、治疗后7 d的神经损伤标志物[中枢神经特异性蛋白(S100β)、神经元特异性烯醇化酶(NSE)]、大脑动脉血流动力学指标[颈总动脉血流平均流速(Vmean)、最大流速(Vmax)、血管阻力指数(RI)、搏动指数(PI)],并观察两组治疗后7 d内并发症发生情况。结果治疗后7 d,观察组治疗总有效率(92.59%,50/54)高于对照组(77.78%,42/54),P0.05)。结论血管内机械再通术联合重组组织型纤溶酶原激活剂静脉溶栓治疗可改善急性脑梗死患者的大脑血流动力学指标,减少神经损伤,提高临床疗效。

关键词：脑梗死重组组织型纤溶酶原激活剂静脉溶栓血管内机械再通术

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