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Evaluation of non-covalent interaction between Seryl-histidine dipeptide and cyclophilin A using NMR and molecular modeling

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science china Chemistry 2010年第9期53卷 1987-1993页

作者： LIU Yan1,ShI Yanhong2,LIU XiaoXia1,LIN MingKun1,LIN Donghai1,2 & ZhAO YuFen1,3 1Key Laboratory for Chemical biology of Fujian province,department of Chemical biology,department of Chemistry,college of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,china 2Shanghai Institute of Materia Medica,Shanghai Institutes of biological sciences,Chinese Academy of sciences,Shanghai 201203,china 3Key Laboratory for Bioorganic phosphorus Chemistry and Chemical biology,Ministry of Education,department of Chemistry,School of Life sciences and Engineering,Tsinghua University,Beijing 100084,china 1. Key Laboratory for Chemical biology of Fujian province Department of Chemical Biology Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China2. Shanghai Institute of Materia Medica Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 201203 China3. Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing 100084 China

Seryl-histidine dipeptide(Ser-his) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as *** protein cleavage mechanism has not been addressed *** an initial step of protein cleavage activity,the non-covalent binding affinity of Ser-his for proteins is a crucial *** this work,we took cyclophilin A(CypA) as a substrate protein,and evaluated the non-covalent interaction between CypA and Ser-his using a combination of NMR spectroscopy and molecular modeling *** independent Ser-his binding sites on CypA were detected using 15N-1h heteronuclear single-quantum coherence(hSQC) *** binding site binds one Ser-his *** constants,Kd1 and Kd2,were estimated to be 2.07 and 6.66 mmol/L,respectively,indicative of the weak non-covalent interaction between Ser-his and *** on molecular modeling results,we suggest that both the α-amino and the side chain hydroxyl group of Ser-his are crucial for the non-covalent interaction between Ser-his and *** work sheds light on the molecular mechanism of Ser-his and its analogues cleaving proteins.

关键词： Ser-his cyclophilin A hSQC non-covalent interaction binding sites

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Establishment of Master Cell Stock and Working Cell Bank of MDCK Lines and Selection and Evaluation of the Lines as Candidate Viral Substrates for Approval production of Combinational Canine Attenuated-live Virus Vaccines

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Agricultural sciences in china 2002年第9期1卷 1054-1066页

作者： ZhANG De-li, LI Liu-Jin, XIA Geng-tian, hE Xu-yu, GAO Bu-xian, BAI Xiao-hong, hUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de( department of Immunology , School of Basic medical sciences, peking University health science Center , Beijing 100083 , p. R . china , Beijing Institute of preventive Medicine, Beijing 100071, p. R . china department of Toxicology at Center for Research and Development of Animal healthcare products , Fourth Military medical University , Xi’an 710032 , p. R . china department of pathology at School of pre-clinical Education , Section of pathology at Xi-Jing hospital, Institute for Cancer Research at china Cancer Research Foundation , Fourth Military medical University, Xi’an 710032 , p. R . china Key Laboratory of preventive Veterinary Medicine of State Ministry of Agriculture , college of Animal medical sciences and State Key Laboratory of Agricultural Biotechnology, college of biological sciences , china Agricultural University, Beijing 100094 , p.R.china State Key Laboratory of medical Molecular biology, School of Basic medical sciences at the peking Union medical Collage /Institute of Basic medical sciences at the Chinese Academy of medical sciences , Beijing 100005 , p. R . china ) department of Immunology School ofBasic Medical Sciences Peking University Health Science Center Beijing 100083 P.R. China Beijing Institute of preventive Medicine Beijing 100071 P. R. China department of Toxicology at Center for Research and Devel department of Toxicology at Center for Research and Development of Animal healthcare products Fourth Military Medical University Xi'an 710032 P. R. China Beijing Institute of preventive Medicine Beijing 100071 P. R. China department of pathology at School of pre-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xi'an 710032 P. R. China Key Laboratory of preventive Veterinary Medicine of State Ministry of Agriculture College of Animal Medical Sciences and State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural UniversityBeijing 100094 P. State Key Laboratory of medical Molecular biology School of Basic Medical Sciences at the Peking Union Medical Colloge /Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P. R . China

Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with di-and hy-perploid YB strain of MDCK cell during 17 - 23 passages, with hyper- and hypoploid KA strain of MDCK cell during 6-8 passages, with hypoploid WB strain of MDCK cell on passage 6, with hyper-and hypoploid h strain of MDCK cell during 8-24 passages was 2/24, 6/10, 5/10 and 10/15, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)passages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome kary-otype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. It is thus evident that MDCK cell of WB or h strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be approved as substrate for the preparation of attenuated viral vaccines.

关键词： Cell line MDCK Master cell stock Working cell bank Karyotypes Carcinogenesis Tu-morigenicity

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Intestinal digestibility of protein and amino acid of ruminant feeds with the mobile nylon bag and in vitro digestion technique

Intestinal digestibility of protein and amino acid of rumina...

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The Ⅶ International Symposium on the Nutrition of herbivores(ISNh-7)

作者： Q.Yue~1 h.J.Yang~(1 3) Ch.Y.Xie~1 X.B.Yao~1 J.Q.Wang~2 ~1department of Animal biology & physiology college of biological sciences china Agricultural University Beijing 100094 p.R.china ~2State Key Laboratory of Animal Nutrition Institute of Animal science china Academy of Agricultural sciences Beijing 100094 p.R.china

＜正＞The study was to provide the Chinese Feedbank with intestinal digestibility（Idg）of crude protein（Cp）and AA for feedstuffs commonly used in *** various AA of feedstuffs weren’t synchronously digested,while a larger variation was found in the same AA between *** Cp Idg estimated by mobile nylon bag technique in soyabean meal,cottonseed meal,peanut cake meal,rapeseed meal,rice bran,maize germ meal,wheat bran and lucerne hay were 0.964,0.813, 0.950,0.826,0.586,0.793,0.679 and 0.400,*** has a strong correlation with the value obtained by three-step in vitro procedure.

关键词： crude protein amino acid intestinal digestibility ruminants

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Selenium-dependent glutathione peroxidase(Se-Gpx) expression during gonad maturation and its response to LpS and h202 challenge in Scylla paramamosain

Selenium-dependent glutathione peroxidase(Se-GPx) expression...

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2011年中国水产学会学术年会

作者： Mingjun Fu~(1,2),Zhihua Zou~(1,2),Shengfa Liu~1,peng Lin~2,Yilei Wang~(2*),Ziping Zhang~3 1 department of biology,Xiamen University,Xiamen,china,361005 2 Fisheries college,Jimei University,Xiamen,china.361021 3 department of biological science,Seton hall University,New Jersey,USA,07079

A selenium-dependent glutathione peroxidase cDNA was obtained from haemocyte of green mud crab Scylla paramamosain(SpGpx) by homology polymerase chain reaction(pCR) technique and rapid amplification of cDNA ends(RACE) *** 1135 bp cDNA contains a 9 bp 5’-untranslated region(UTR),an open reading frame(ORF) of 564 bp encoded a deduced amino acid sequence of 187 amino acids(aa),and a 562 bp 3’-untranslated region with a 100 bp conserved eukaryotic selenocysteine insertion sequence(SECIS).It involves a putative selenocysteine(Sec,or U) residue which is encoded by an opal codon,TGA,and forms an active site with residues Q and *** characterization revealed that SpGpx contain a characteristic Gpx signature motif 2(LAFpCNQF),an active site motif(WNFEKF),a potential N-glycosylation site(NTT),and two residues(R and R) which contribute to the electrostatic architecture by directing the glutathione donor *** sequence alignment and phylogenetic analysis showed that SpGpx shares a high level of similarity with other selected invertebrate Gpxs and vertebrate Gpxl and *** mRNA expression can be detected in various tissues,and higher-level expression of SpGpx was detected in the tissues of gill and *** SpGpx expression was increased and then slightly declined during ovarian and testicular *** might play an important role in gonadal development by protecting tissues from oxidative *** expression of SpGpx mRNA was induced by lipopolysaccharide (LpS) and hydrogen peroxide(hO) in ***,it was not significantly changed by comparing with control group in gill and *** results suggested that SpGpx was implicated in the immune response induced by LpS and hO.

关键词： SpGpx gonadal development LpS h2O2 gene expression Scylla paramamosain

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DESCRIpTION OF TWO NEW SpECIES OF hALIOTREMA JOhNSTON ＆TIEGS （MONOGENEA, ANCYROCEphALIDAE）

DESCRIPTION OF TWO NEW SPECIES OF HALIOTREMA JOHNSTON ＆TIEG...

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第十九届国际动物学大会

作者： hai-Yan Lidepartment of biology college of biology & Chemistry engineering Guangzhou University Guangzhou 510405 china

＜正＞Two new species of monogenegs were collected: haliotrema neokurodai, sp. nov. ＆ h. flexiosus , ***. from the gills of a marine eleoridid, Sparus macrocephalus （Basilewsdy）. They are attributed to the genus ha...

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古尼拟青霉胞内多糖的提取分离工艺及条件

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山地农业生物学报 2003年第2期22卷 140-145页

作者：肖建辉梁宗琦刘爱英遵义医学院附属医院贵州省细胞工程重点实验室贵州遵义563003 贵州大学生物与环境科学学院生物技术系贵州贵阳550025

通过对古尼拟青霉胞内多糖(简称pGpS)的提取分离工艺及条件的研究,结果表明,正交试验优化后的pGpS提取条件:一份脱脂菌粉,加10倍菌粉量的蒸馏水,70℃条件下浸提2h,浸提3次,合并浸提液并浓缩至其总体积的1/6,再加至质量分数85%的乙醇,醇... 详细信息

通过对古尼拟青霉胞内多糖(简称pGpS)的提取分离工艺及条件的研究,结果表明,正交试验优化后的pGpS提取条件:一份脱脂菌粉,加10倍菌粉量的蒸馏水,70℃条件下浸提2h,浸提3次,合并浸提液并浓缩至其总体积的1/6,再加至质量分数85%的乙醇,醇析12h。大孔吸附树脂脱色效果优于h2O2。pGpS半精制品上DEAE-SephadexA-25层析柱和SephadexG-200层析柱获得4个主要组分。pGpS中多糖含量为13 9%,其中含有结合蛋白的多糖组分。

关键词：古尼拟青霉胞内多糖提取工艺分离纯化正交试验古尼虫草药物

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浙江北部沿海婆罗囊螺的生物学特征

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水产学报 2003年第4期27卷 343-349页

作者：王志铮张义浩李太武李仁伟孙爱丽浙江海洋学院渔业学院浙江海洋学院科研处宁波大学生命科学与生物工程学院舟山市水产养殖技术研究开发中心宁波大学生命科学与生物工程学院浙江舟山　316004 浙江宁波　315040 浙江舟山　316000

根据2001年5月至2002年5月间浙江北部沿海逐月采集所得1146颗婆罗囊螺生物学测定资料,并结合该螺野外生物学观察资料进行生长生物学综合分析。结果表明,该螺为软泥相潮间带数量优势种,垂直分布差异显著。喜阳,常出没于较干燥的海涂表面... 详细信息

根据2001年5月至2002年5月间浙江北部沿海逐月采集所得1146颗婆罗囊螺生物学测定资料,并结合该螺野外生物学观察资料进行生长生物学综合分析。结果表明,该螺为软泥相潮间带数量优势种,垂直分布差异显著。喜阳,常出没于较干燥的海涂表面,栖息地受潮汐、光照和饵料分布调控;属明显集群繁殖类型,亲体产后大多死亡,一年生;5-9月为繁殖期,以6-7月为盛,6月下旬开始有幼螺群体出现,其生长速率在当年7-9月和次年4-6月各有一个高峰,11月-次年2月基本停止生长,肥满度以次年6月初为最高;壳高(h)与壳宽(B)成线性相关,其方程为B=0.3224+0.4916h(r=0.9770),壳高与鲜重、干重呈幂函数相关,其回归关系方程分别为W湿=2.1×10-4L2.8352(r=0.8448)、W干=2.97×10-4L2.2803(r=0.8939);最后,还就杀灭婆罗囊螺的时机进行了探讨。

关键词：浙江北部沿海婆罗囊螺生长特征生物学

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赤水景区旅游气候资源分析与评价

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贵州大学学报（农业与生物科学版） 2002年第5期21卷 320-326,331页

作者：徐向华穆彪何佩云熊中华贵州大学林学院林学系贵州贵阳550025 贵州大学生物与环境科学学院贵州贵阳550025

经对赤水市旅游景区部分景点的考察和资料订正表明 ,该景区各点太阳辐射年总量为2 4 6 8.0～ 314 2 .2MJ/m2 ,年实照时间 4 5 1.5～ 86 9.2h ,年日照百分率为 9.7%～ 18.6 % ,为贵州省乃至全国最低值区之一 ;年平均气温为 14 .1～ 17.4... 详细信息

经对赤水市旅游景区部分景点的考察和资料订正表明 ,该景区各点太阳辐射年总量为2 4 6 8.0～ 314 2 .2MJ/m2 ,年实照时间 4 5 1.5～ 86 9.2h ,年日照百分率为 9.7%～ 18.6 % ,为贵州省乃至全国最低值区之一 ;年平均气温为 14 .1～ 17.4℃ ,年降水量 899.7～ 16 6 1.0mm ,年均相对湿度 81%左右 ,年均绝对湿度 14 .2～ 17.3hpa ;景区各点地形遮蔽 ,多云寡照 ,冬无严寒、夏无酷暑 ,气候宜人 ;根据生物气候指数可知 :赤水景区可全年开展旅游业 ,4～ 10月是旅游的舒适季节 ,其中5、6。

关键词：赤水市旅游景区森林太阳辐射温度湿度降水量生物气候指数气候资源评价

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赤水、习水沟谷地区的日照和太阳辐射状况

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贵州大学学报（农业与生物科学版） 2002年第6期21卷 404-408页

作者：何佩云穆彪徐向华熊中华贵州大学林学院林学系贵州贵阳550025 贵州大学生物与环境科学学院贵州贵阳550025

通过地形遮蔽图法对赤水、习水沟谷地区的日照和太阳辐射进行了测算 ,结果表明 ,该区年太阳总辐射为 2 4 6 6 3～ 2 999 2MJ/m2 ,较当地开阔地形站减少 2 1%～ 35 % ,年实照时间为4 5 1 2～ 786 9h ,较当地开阔地形站减少 39%～ 6 5 ... 详细信息

通过地形遮蔽图法对赤水、习水沟谷地区的日照和太阳辐射进行了测算 ,结果表明 ,该区年太阳总辐射为 2 4 6 6 3～ 2 999 2MJ/m2 ,较当地开阔地形站减少 2 1%～ 35 % ,年实照时间为4 5 1 2～ 786 9h ,较当地开阔地形站减少 39%～ 6 5 % ,年日照百分率为 10 2 %～ 17 8%。 7、8月晴朗天气多 ,太阳高度角大 ,地形遮蔽作用小 ,实照时间较当地开阔地形站减少 35 %～ 6 3% ;12月阴雨天气多 ,太阳高度角小 ,地形遮蔽作用大 ,实照时间减少 5 3%～ 84 %。沟谷地区阴蔽 ,阳光和煦 ,冬暖夏凉 ,湿润风和。

关键词：赤水习水沟谷地区日照贵州太阳辐射状况

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A Brassica carinata pan-genome platform for Brassica crop improvement

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plant Communications 2024年第1期5卷 20-23页

作者： Yan Niu Qingqing Liu Zhesi he Rosy Raman hao Wang Xinxin Long han Qin harsh Raman Isobel A.p.parkin Ian Bancroft Jun Zou National Key Laboratory of Crop Genetic Improvement College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina department of biology University of YorkHeslingtonYork YO105DDUK NSW department of primary Industries Wagga Wagga Agricultural InstituteWagga WaggaNSW 2650Australia Agriculture and Agri-Food Canada Saskatoon Research and Development CentreSaskatoonSKCanada

Dear Editor,Brassica carinata(Bc Bc Cc Cc),an important agricultural crop cultivated for the edible oil,leafy vegetable,high-value protein stockfeed,and biofuel markets,contains favorable genetic variation that can be exploited to develop climate-resilient and wateruse-efficient Brassica crops(Seepaul et al.,2021).Although several quantitative trait loci(QTLs)for flowering time,seed quality traits,and pod shatter resistance(pSR)have been identified based on high-density genetic linkage maps(Zou et al.,2014;Raman et al.,2017;Zhang et al.,2017),this information alone is insufficient for practical genetic improvement and breeding applications.

关键词： breeding crops cultivated

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