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Study on intracellular trafficking of Mac-1 by direct visualization

Science china(Life Sciences) 2004年第6期47卷 521-529页

作者： YAN Ming1, MAO Jifang2, WEI Yi3, ZHONG Jigen1, YANG Shengsheng2 & XU Renbao1 1. department of Pathophysiology, second military medical university, shanghai 200433, china 2. department of Biochemistry and Molecular Biology, second military medical university, shanghai 200433, china 3. Biological Science School, Fudan university, shanghai 200433, china Correspondence should be addressed to Yan Ming 1. department of Pathophysiology Second Military Medical University 200433 Shanghai China 2. department of Biochemistry and Molecular Biology Second Military Medical University 200433 Shanghai China 3. Biological Science School Fudan University 200433 Shanghai China

Previously, we constructed DNA vectors containing cDNA of Mac-1 subunits (CD11b or CD18b) fused with fluorescence protein (FP). cDNA fragments and the DNA constructs were then transfected into CHO cells (as CHO-Mac-1-FP). The structure and function of Mac-1-FP obtained from the CHO-Mac-1-FP cells are nearly identical to that expressed in wild type leuko- cytes. In the present study, the intracellular trafficking of Mac-1 was visualized directly by moni- toring the fluorescent intensities of YFP-CD18 and PE-conjugated monoclonal antibody against CD11b under a confocal microscope in CHO-Mac-1-FP cells. The results indicate that: (ⅰ) al- though Mac-1 was not detected in the cell membrane at resting state, it had been translocated and clustered into the cell membrane by 1 h and internalized 2 h after PMA stimulation, at which point the fluorescence intensity began to diminish gradually, probably due to partial degradation of Mac-1. The fluorescence of CD18 and CD11b reappeared on the cell membrane 1 h after re-treatment with PMA, suggesting the recycling of non-degraded Mac-1. (ⅱ) The adhesion rate of CHO-Mac-1-FP to magnetic beads coupled ICAM-1 increased within 4 h after their initial in- teraction, accompanied by the clustering of Mac-1-FP. After 8 h,the adhesion rate declined and fluorescence also decreased simultaneously. The pattern of change in fluorescence in CHO-Mac-1-FP cells elicited by ICAM-1 beads was similar to that elicited by PMA, suggesting that endocytosis and degradation of Mac-1 occurred after the interaction with ICAM-1. Thus, we conclude that the intracellular trafficking of Mac-1 after activation is associated with membrane translocation, endocytosis, degradation and recycling. These changes are in parallel with the adhesion of CHO-Mac-1-FP cells with ICAM-1, and may be involved in the adhesion and de- tachment of leukocytes. The detachment of leukocytes may be caused by endocytosis of Mac-1.

关键词： Mac-1 (macrophage differentiation antigen associated with complement three receptor function), PMA, ICAM-1, trafficking.

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Effects of Changtai granules, a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats

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World Journal of Gastroenterology 2005年第23期11卷 3539-3543页

作者： Yong-Bing Cao,Jun-Dong Zhang,Lan Yan,De-Jun Wang,Xin-Ming Jia,Ping-Hui Gao,Ming-He Cheng,Zheng Xu,Yan Wang,Yuan-Ying Jiang,department of Pharmacology,School of Pharmacy,second military medical university,shanghai 200433,china Ya-Ying Diao,department of Pharmacy,Changhai hospital,second military medical university,shanghai 200433,china department of Pharmacology School of Pharmacy Second Military Medical University Guohe Road 325 Shanghai 200433 China.

AIM: To study the effects of Changtai granules (CTG), a traditional compound Chinese medicine, on chronic trinitrobenzene sulfonic acid-induced colitis in rats. METHODS: Healthy adult Sprague-Dawley (SD) rats of both sexes, weighing 250-300 g, were employed in the present study. The rat colitis models were induced by 2, 4,6-trinitrobenzene sulfonic acid (TNBS) enemas at a concentration of 100 mg/kg in 50% ethanol. The experimental animals were randomly divided into dexamethasone (DX) treatment, CTG treatment, and model control groups, which were intracolicly treated daily with DX (0.2 mg/kg), CTG at doses of 2.9, 5.7 and 11.4 g crude drug/kg, and the equal amount of saline respectively from 6 h following induction of the colitis in rats inflicted with TNBS to the end of study. A normal control group of rats treated without TNBS but saline enema was also included in the study. After 3 wk of treatment, the animals were assessed for colonal inflammatory and ulcerative responses with respect to mortality, frequency of diarrhea, histology and myeloperoxidase activity (MPO).RESULTS: The therapeutic effect of CTG on ulcerative colitis (UC) was better than DX. CTG effectively inhibited the activity of granulocytes, macrophages and monocytes in a dosedependent manner. Also it reduced MPO and formation of inflammation in colonic mucosal tissue. Furthermore, administration of CTG significantly prevented body mass loss and death, and decreased frequency of diarrhea in UC rats, when compared with the model control group ***: CTG would prove to be an ideal drug for chronic UC, and is warranted to be studied further.

关键词： Ulcerative colitis Changtai granules 2,4,6-Trinitrobenzene sulfonic acid Myeloperoxidase

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Visfatin derived from perivascular adipose tissue promotes vascular smooth muscle cell proliferation

Visfatin derived from perivascular adipose tissue promotes v...

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中国药理学会第十次全国学术会议

作者： WANG Pei,MIAO Chao-yu~* (department of Pharmacology,second military medical university,shanghai 200433,china)

Perivascular adipose tissue(PVAT) inhibits vascular smooth muscle cell(VSMC) contraction and induces VSMC proliferation by releasing protein *** present study was to determine whether visfatin is involved in these par...

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Effects of combination therapy with atenolol and amlodipine on blood pressure control and stroke prevention in stroke-prone spontaneously hypertensive rats

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Acta Pharmacologica Sinica 2007年第11期28卷 1755-1760页

作者： Gang LING,Ai-jun LIU,Fu-ming SHEN,Guo-jun CAI,Jian-guo LIU,Ding-feng SU~2department of Pharmacology,School of Pharmacy second military medical university,shanghai 200433,china ment of Pharmacology School of Pharmacy Second Military Medical University Shanghai 200433 China. dfsu@***.

Aim:To test the effects of atenolol and amlodipine,either alone or in combination,on blood pressure,blood pressure variability (BPV),baroreflex sensitivity (BRS),and the prevalence of stroke in stroke-prone spontaneously hypertensive rats(SHR-SP).Methods:In the first set of the study,24 8-month-old,female SHR-SPrats were randomly divided into 3 *** pressure,heart period,and BRSwere determined before and after the intragastric administration of atenolol (10mg/kg) and amlodipine (1.0 mg/kg),either alone or in *** the secondset of the study,40 male and 40 female rats were randomly assigned to 1 of thefollowing 4 groups:control,atenolol (10 mg·kg-1·d-1),amlodipine (1.0 mg·kg-1·d-1),and both (10 male and 10 female in each group).The stroke incident and survivaltime were ***:Atenolol and amlodipine,either alone or incombination,significantly decreased blood pressure,with the exception of theamlodipine-induced effect on diastolic blood ***,only the com-bination treatment significantly decreased the BPV levels for the same *** q-values calculated by the probability sum analysis were 1.17 and 2.67 forsystolic and diastolic blood pressure,respectively,and were 2.48 and 2.10 forsystolic and diastolic BPV,respectively,following *** drugexhibited any significant effect on *** and amlodipine,either alone orin combination,significantly increased the lifespan of SHR-SP,with the best ef-fect elicited by the combination ***:A significant synergismexists between atenolol and amlodipine in lowering and stabilizing blood pressurein *** therapy may be an optimal strategy for the prevention ofstroke in hypertension.

关键词： stroke hypertension stroke-prone spontaneouslyhypertensive rats atenolol amlodipine blood pressure blood pressure variability

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Relation between upregulation of CD40 system and complex stenosis morphology in patients with acute coronary syndrome

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Acta Pharmacologica Sinica 2004年第2期25卷 125-130页

作者： Jin-chuan YAN2, ZONG-gui WU3, Xian-tao KONG3, Ren-qian ZONG3, Lin-zen ZHAN3 department of Cardiology, Affiliated Zhong Da hospital, Southeast university, Nanjing 210009 3 changzheng hospital, second military medical university, shanghai 200003, china department of Cardiology Affiliated Zhong Da Hospital Southeast University Nanjing 210009 Chin

AIM: To investigate whether upregulation of CD40-CD40 ligand system is related to matrix metalloproteinases level and stability of coronary atherosclerotic plaque in patients with acute coronary syndrome (ACS). METHODS: Sixteen normal controls and 56 patients including 24 with stable angina (SA), 20 with unstable angina (UA), and 12 with acute myocardial infarction (AMI) were investigated. The expression of CD40 and CD40L on platelet was analyzed by flow cytometry. Serum soluble CD40L (sCD40L), MMP-9 and MMP-3 level was determined by ELISA. All coronary stenosis with ≥30 % diameter reduction were assessed by angiographic coronary stenosis morphology. RESULTS: Patients with ACS showed a significant increase of CD40 (75±12 MIF) and CD40L (13±4 MIF) coexpression on platelets compared with control and SA group (P2±3.5 μg/L) than in control (3.1±1.4 μg/L, P<0.01) and SA group (3.3±1.6 μg/L, P<0.01). Serum MMP-3 and MMP-9 in patients with ACS were two times greater than those in control. A positive correlation was found between MMP-9, MMP-3, and CD40L expression on platelets as well as sCD40L levels, but not for CD40 expression on platelets. An obvious correlation was also observed between sCD40L concentration and complex coronary stenoses (r=0.60, P<0.01). CONCLUSION: Patients with ACS show increased coexpression of CD40 system, especially expression of CD40L, which may create a proinflammatory and prothrombotic milieu for aggravating the development of atherosclerosis and instability of atherosclerotic plaques, and may be a valuable marker for predicting the severity of ACS.

关键词： CD40 antigens CD40 ligand coronary disease matrix metalloproteinases

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中国乙型肝炎病毒母婴传播防治指南(2024年版)

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中华肝脏病杂志 2024年第8期32卷 702-711页

作者：中国医师协会感染科医师分会中华医学会感染病学分会赵英仁李兰娟张文宏刘锦锋曾庆磊纪泛扑不详西安交通大学第一附属医院感染性疾病科西安710061 浙江大学医学院附属第一医院传染病重症诊治全国重点实验室杭州310003 复旦大学附属华山医院感染科上海200040 郑州大学第一附属医院感染病科西安交通大学第二附属医院感染性疾病科

中华医学会感染病学分会制定的《中国乙型肝炎病毒母婴传播防治指南(2019年版)》(简称2019版《指南》)对国内乙型肝炎病毒母婴传播阻断流程的规范化起到了良好的指导作用。临床实践指南和共识需要随新研究证据的出现,及时更新以更好地... 详细信息

中华医学会感染病学分会制定的《中国乙型肝炎病毒母婴传播防治指南(2019年版)》(简称2019版《指南》)对国内乙型肝炎病毒母婴传播阻断流程的规范化起到了良好的指导作用。临床实践指南和共识需要随新研究证据的出现,及时更新以更好地指导临床实践和研究。中国医师协会感染科医师分会和中华医学会感染病学分会联合多学科专家,基于国内外最新研究进展,结合临床实践,对2019版《指南》进行了更新和补充,为临床医师和妇幼保健工作人员提供指导和参考。

关键词：乙型肝炎病毒母婴传播治疗预防指南更新

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Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract

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Acta Pharmacologica Sinica 2005年第7期26卷 835-839页

作者： Ya-bin JIAO~2 Yao-cheng RUI~3 Tie-jun LI Peng-yuan YANG Yan QIU department of Pharmacology,School of Pharmacy,the second military medical university,shanghai 200433,china 2 Now in department of Pharmacology,School of Pharmacy,shanghai university of Traditional Chinese Medieine,shanghai 201203,china. NowindepartmentofPharmacology SchoolofPharmacyShanghaiUniversityofTraditionalChineseMedicineShanghai201203China

Aim:To study the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in the brain of rats with atherosclerosis(AS)and theeffects of Ginkgo biloba extract(GbE)on expressions of ***:The expermental model of AS in rats were established by intraperitioneal injectionof vitamin D3 with high fat/cholesterol *** 100 mg/kg was administered torats by *** 8 weeks,the expressions of IL-1β,TNF-α,IL-10,and 1L-10R in thebrain tissues of AS rats were detected by enzyme-linked immunosorbant assay,immunohistochemistry,Western blotting,and reverse transcriptase polymerasechain ***:The protein and mRNA expressions of IL-1β,TNF-α,andIL-10 in the brains were markedly higher in AS groups than that in control groups(6.11±0.15,1.55±0.14,0.54±0.04 ng/g wet weight vs 0.80±0.14,0.33±0.09,and0.33±0.02 ng/g wet weight,respectively).The protein and mRNA expres-sions of IL-1β and TNF-α in the brains were markedly lower in GbE groups(3.82±0.54,0.95±0.08 ng/g wet weight)than that in AS groups,the protein andmRNA expressions of IL-10 and 1L-10R in the brains were markedly higher in GbEgroups(0.85±0.06 ng/g wet weight)than that in AS ***:GbEinhibited production of pro-inflammatory cytokines IL-1β and TNF-α,but up-regulated the production of anti-inflammatory cytokines,1L-10 and IL-10R in brain,which might be related with its anti-AS actions.

关键词： atherosclerosis cytokine interleukin-1 tumor necrosis factor interleukin-10 Ginkgo biloba rats

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转基因的分子生物学特性

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生物化学与生物物理进展 1996年第2期23卷 137-141页

作者：戴旭明潘星华傅继梁第二军医大学生物学教研室

现代分子生物学研究中，转基因概念的出现越来越频繁，它渐渐被用来表示所有的用基因工程手段构建、导入高等真核生物细胞，并稳定地整合入受体基因组的外源ＤＮＡ．文章较系统地总结了已见报道的转基因的结构类型、它在受体细胞内的遗... 详细信息

现代分子生物学研究中，转基因概念的出现越来越频繁，它渐渐被用来表示所有的用基因工程手段构建、导入高等真核生物细胞，并稳定地整合入受体基因组的外源ＤＮＡ．文章较系统地总结了已见报道的转基因的结构类型、它在受体细胞内的遗传学行为、表达特性和影响因素及其相应的生物学效应；分析了转基因研究中尚待研究的问题。

关键词：转基因分子生物学转基因生物

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Antioxidant activities of α-methyl-4-(3-oxo-2H-1,2-benzoisoselenazol-2-yl)benzeneacetic acid in vitro

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中国药理学与毒理学杂志 1998年第1期 68-69页

作者： ZENG HuaWu, JIANG YuanYing1, CAI DingGuo, TAO ZhiHua, LONG Kun1(The Pharmacy Center of Navy, The Navy 411 hospital, shanghai 200081 1 department of Pharmacology,College of Pharmacy, The second military medical university, Shangh

Ａｎｔｉｏｘｉｄａｎｔａｃｔｉｖｉｔｉｅｓｏｆα┐ｍｅｔｈｙｌ┐４┐（３┐ｏｘｏ┐２Ｈ┐１，２┐ｂｅｎｚｏｉｓｏｓｅｌｅｎａｚｏｌ┐２┐ｙｌ）ｂｅｎｚｅｎｅａｃｅｔｉｃａｃｉｄｉｎｖｉｔｒｏＺＥＮＧＨｕａ－Ｗｕ，ＪＩ... 详细信息

Ａｎｔｉｏｘｉｄａｎｔａｃｔｉｖｉｔｉｅｓｏｆα┐ｍｅｔｈｙｌ┐４┐（３┐ｏｘｏ┐２Ｈ┐１，２┐ｂｅｎｚｏｉｓｏｓｅｌｅｎａｚｏｌ┐２┐ｙｌ）ｂｅｎｚｅｎｅａｃｅｔｉｃａｃｉｄｉｎｖｉｔｒｏＺＥＮＧＨｕａ－Ｗｕ，ＪＩＡＮＧＹｕａｎ－Ｙｉｎｇ１，ＣＡＩ...

关键词： αmethyl4(3oxo2H1,2benzoisoselenazol2yl) benzeneacetic acid selenoorganic compound lipid peroxides

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Mastoidectomy via a retro-labyrinthine approach aided by 3D CT image reconstruction of the temporal bone: an anatomic study

Mastoidectomy via a retro-labyrinthine approach aided by 3D ...

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2011中华医学会神经外科学学术会议

作者： Huanhai Liu1 Junyu Wang2 Qi He3 Lijun Hou2 Yicheng Lu2 Jianchun Liao1 Ruishan Dang4 1.department of Otorhinolaryngology, changzheng hospital of the second military medical university, shanghai, china 2.shanghai Neurosurgical Institute, department of Neurosurgery, changzheng hospital of the second military medical university 3.department of Radiology, changzheng hospital of the second military medical university 4.department of Human Anatomy, the second military medical university

Background The semicircular canal and mastoid segment of facial nerve embedded in the mastoid air cells are susceptible to injury in microsurgery via the transpetrosal approach, especially for neurosurgery residents w... 详细信息

关键词： mastoidectomy retrolabyrinthine approach high spatial resolution CT temporal bone anatomy

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