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Prevention of pathological change and cognitive degeneration of Tg2576 mice by inoculating Aβ_(1-15) vaccine

Science china(Life Sciences) 2008年第8期51卷 743-750页

作者： HU JinJia1, LI GuoYing2, WANG HuaQiao3, LIN Xian3 & YAO ZhiBin3 1 department of anatomy, Medical college of Shanghai Jiao Tong university, Shanghai 200025, china 2 department of anatomy, preclinical college, guangdong university of pharmacy, guangzhou 510006, china 3 department of anatomy, Zhongshan School of Medicine, Sun Yat-sen university, guangzhou 510080, china department of anatomy Medical College of Shanghai Jiao Tong University Shanghai China department of anatomy Preclinical College Guangdong University of Pharmacy Guangzhou China department of anatomy Zhongshan School of Medicine Sun Yat-sen University Guangzhou China

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2576 mice by inoculating the subunit fragment of Aβ ***-two Tg2576 mice were randomly divided into four groups, each having eight mice:Group I, the control group, inoculated with adjuvants;Group II, the Aβ42 group, inoculated with Aβ42 vaccine;Group Ⅲ, the Aβ1―15 group, inoculated with Aβ1―15 vaccine;and Group IV, the Aβ36―42 group, inoculated with Aβ36―42 *** titer of the serum anti-body against Aβ42(Group II) was significantly higher than that of the control group(Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated *** water maze test showed that the Aβ42 group, Aβ1―15 group and Aβ36―42 group were obviously im-proved compared with the control *** cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control *** the Aβ42 group, IL2 and IFN-γ were relatively low and IL4 and IL10 were relatively *** contrast, IL4 and IL10 were much higher in the Aβ1―15 group and IL2 and IFN-γ were much higher in the Aβ36―42 *** immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the con-trol group, no senile plaque in the brain of the Aβ1―15 group and Aβ42 group mice, and a small number of senile plaques in the brain of the Aβ36―42 group *** results suggest that the subunit fragment of Aβ1―15 vaccine could prevent not only cognitive and behavioral degeneration but also Aβ deposition and formation of senile plaques in Tg2576 mice.

关键词： Alzheimer’s disease β-amyloid peptide vaccine Tg2576 mice senile plaque

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Pharmacokinetic behaviors and oral bioavailability of oridonin in rat plasma

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Acta Pharmacologica Sinica 2006年第12期27卷 1642-1646页

作者： Wen XU~2 Jin SUN~2 Ting-ting ZHANG~2 Bo MA~2 Sheng-miao CUI~3 Da-wei CHEN~2 Zhong-gui HE~(2,4)2 department of Pharmaceutics,School of pharmacy,Shenyang Pharmaceutical university,Shenyang 110016,china 3 department of pharmacy,guangdong Pharmaceutical university,guangzhou 510006,china department of Pharmaceutics School of Pharmacy Shenyang Pharmaceutical University Shenyang China department of pharmacy Guangdong Pharmaceutical University Guangzhou China

Aim:To study the intravenous and oral pharmacokinetic behavior of oridonin andits extent of absolute oral bioavailability in ***:Oridonin was adminis-tered to rats via iv(5,10 and 15 mg/kg),po(20,40 and 80 mg/kg)or ip administra-tion(10 mg/kg).The concentrations of oridonin in rat plasma were determined bya high performance liquid chromatography with electrospray ionization mass spec-trometric detection(HPLC/ESI-MS)method and the pharmacokinetic parameterswere determined by non-compartmental ***:The plasma concen-tration of oridonin after intravenous administration decreased polyexponentially,and the pharmacokinetic parameters of oridonin were dose-independent withinthe examined *** was absorbed rapidly after oral gavage with a t(max)ofless than 15 min;the extent of absolute bioavailability of oridonin following oraladministration was 4.32%,4.58% and 10.8%.The extent of absolute bioavailabilityof oridonin following intraperitoneal administration was 12.6%.Conclusion:Firstorder rate pharmacokinetics were observed for oridonin within the range of ivdoses,while the extent of absolute oral bioavailability was rather low and *** low and dose-dependent extent of oral bioavailability may be dueto the saturation of first-pass effects.

关键词： oridonin pharmacokinetics intravenous oral bioavailability intraperitoneal injections rats

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Three-Electron Quantum Dots in Zero Magnetic Field

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Communications in Theoretical Physics 2007年第12期48卷 1115-1118页

作者： XIE Wen-Fang department of Physics,guangzhou university,guangzhou 510006,china department of Physics Guangzhou University Guangzhou 510006 China

By using the exact diagonalization method,a system of three electrons confined in a parabolic quantumdot in zero magnetic field is *** ground-state electronic structures and orbital and spin angular momentatransitions as a function of the confined strength are *** find that the confinement may cause accidentaldegeneracies between levels with different low-lying states and the inversion of the energy *** present,resultsare useful to understanding the optical properties and internal electron-electron correlations of quantum dot materials.

关键词： electron-electron interaction quantum dot semiconductor

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Expression pattern of antibacterial genes in the Musca domestica

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Science china(Life Sciences) 2009年第9期52卷 823-830页

作者： WANG Yan1,JIN XiaoBao1,ZHU JiaYong1,ZENG AiHua2,CHU FuJiang1,YANG XiaoRong1 & MA Yan1 1 Institute of Pathogen Biology and department of Pathogen Biology,guangdong Pharmaceutical university,guangzhou 510006,china 2 department of Pharmacology,guangdong Pharmaceutical university,guangzhou 510006,china Institute of Pathogen Biology and department of Pathogen Biology Guangdong Pharmaceutical University Guangzhou China department of Pharmacology Guangdong Pharmaceutical University Guangzhou China

This work studied the transcriptional patterns of three antibacterial genes,attacin,defensin and cecropin,during the development of Musca domestica. Quantitative analysis by real-time PCR was performed on mRNA levels in different development stages and challenged 3rd-instar larva at different time points after challenge of Musca domestica. The results revealed a predominance of the transcripts of all three genes during the 3rd-instar larvae and the adults. In the meanwhile,it revealed the greatest increase in mRNA. The transcript levels increased to 801 times,1009 times and 2500 times respectively for cecropin,attacin and defensin in 3rd-instar larvae after challenging susceptible bacterium. The results suggested that the transcriptional patterns of Musca domestica antibacterial genes were different during the different growth stages as well as the microbial challenge encountered in 3rd-instar larvae.

关键词： Musca domestica expression pattern attacin defensin cecropin

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JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release,membrane translocation of Bax and loss of mitochondrial membrane potential

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World Journal of Gastroenterology 2002年第2期8卷 217-223页

作者： Hong-Liang Li Xiao-Hong Li Jun-Hua Lü Xian-Da Ren,department of Pharmacology,Jinan university pharmacy college,guangzhou 510632,guangdong Province,china Dan-Dan Chen,department of Cardiology,First Affiliated Hospital,Zhongshan university,guangzhou 510089,guangdong Province,china Hai-Wei Zhang,department of Pathology,Jinan university Medical college,guangzhou 510632,guangdong Province,china Cun-Chuan Wang,department of laparoscopic surgery,First Affiliated Hospital,Jinan university Medical college,guangzhou 510632,guangdong Province,china department of Pharmacology Jinan University Pharmacy College Guangzhou 510632 Guangdong Province China. tsam@***

AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by *** and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells.

关键词： Adenocarcinoma Stomach Neoplasms Amino Acid Chloromethyl Ketones Anti-Inflammatory Agents, Non-Steroidal Apoptosis Benzenesulfonates Caspases inhibitors Cyclooxygenase Inhibitors Cysteine Proteinase Inhibitors Cytochrome c Group Enzyme Activation Humans In Situ Nick-End Labeling Membrane Potentials Mitochondria Oxazoles Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Research Support, Non-U.S. Gov't Tumor Cells, Cultured bcl-2-Associated X Protein

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Research on Negotiating Agent Development

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Wuhan university Journal of Natural Sciences 2004年第5期9卷 633-637页

作者： WEI Ding-guo 1,2 , PENG Hong 1 1.department of Computer Science, South china university of Technology, guangzhou 510630, guangdong, china 2.guangdong Commercial college, guangzhou 510320,guangdong, china department of Computer Science South China University of Technology Guangzhou Guangdong China guangdong Commercial college Guangzhou Guangdong China

The paper presents a flexible and effective method of development of negotiating agents. A strategy specification, which is specified by a state chart and defeasible rules, can be dynamically inserted into an agent shell incorporating a state chart interpreter and a defeasible logic inference engine, in order to yield a desirable agent. The set of desirable criteria and rules is required to be justified with different context of the application. Key words automatic negotiation - intelligence agent - defeasible logic - state chart CLC number TP 391 Foundation item: Supported by the National Natural Science Foundation of china (60173051)Biography: WEI Ding-guo (1964-), male, Associate professor, research direction: computer network protocal etc.

关键词： automatic negotiation intelligence agent defeasible logic state chart

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Microwave peak absorption frequency of liquid

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Science china(Physics,Mechanics & Astronomy) 2008年第9期51卷 1254-1263页

作者： HAN GuangZe1 & CHEN MingDong2 1 department of Physics,South china university of Technology,guangzhou 510640,china 2 guangzhou Auto college,South china university of Technology,guangzhou 510800,china department of Physics South China University of Technology Guangzhou China guangzhou Auto college South China University of Technology Guangzhou China

Microwave-assisted extraction is a new effective method which has practical ap-plications in many fields. Microwave heating is one of its physical mechanisms,and it also has the characteristic of selectivity. When the applied microwave fre-quency equals a certain absorption frequency of the material (or specific compo-nent),the material will intensively absorb microwave energy. This is also known as resonant absorption,and the frequency is called the peak absorption frequency which depends on the physical structure of the material. In this work,dynamic hy-drogen bond energy was included in molecular activation energy; with the liquid cell model,the expression of interaction energy between dipolar molecules was derived. The rotational relaxation time was gotten from the Eyring viscosity formula. Then based on the relationship between dielectric dissipation coefficient and re-laxation time,the expression of microwave peak absorption frequency as a func-tion of the material physical structure,rotational inertia and electrical dipole mo-ment of molecules was established. These theoretical formulas were applied to water and benzene,and the calculated results agree fairly well with the experi-mental data. This work can not only deepen the study of the interaction between microwave and material,but also provide a possible guide for the experiment of microwave-assisted extraction.

关键词： microwave-assisted extraction microwave heating relaxation time liquid viscosity peak absorption fre-quency

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Changes of NF-kB,p53,Bcl-2 and caspase in apoptosis induced by JTE-522 in human gastric adenocarcinoma cell line AGS cells:role of reactive oxygen species

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World Journal of Gastroenterology 2002年第3期8卷 431-435页

作者： Hong-Liang Li Xiao-Hong Li Yan-Qing L Chun-Ling Ye Xian-Da Ren,department of Pharmacology,Jinan university pharmacy college,guangzhou 510632,guangdong,china Dan-Dan Chen,department of Cardiology,First Affiliated Hospital,Zhongshan university,guangzhou 510089,guangdong,china Hai-Wei Zhang,department of Pathology,Jinan university Medical college,guangzhou 510632,guangdong,china department of Pharmacology Jinan University Pharmacy College Guangzhou 510632 Guangdong China.

AIM: To identify whether JTE-522 can induce apoptosis in AGS cells and ROS also involved in the process, and to investigate the changes in NF-kB, p53, bcl-2 and caspase in the apoptosis process. METHODS: Cell culture, MTT, Electromicroscopy, agarose gel electrophoresis, lucigenin, Western blot and electrophoretic mobility shift assay (EMSA) analysis were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanisms. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Lucigenin assay showed the generation of ROS in cells under incubation with JTE-522. The increased ROS generation might contribute to the induction of AGS cells to apoptosis. EMSA and Western blot revealed that NF-kB activity was almost completely inhibited by preventing the degradation of IkBalpha. Additionally, by using Western blot we confirmed that the level of bcl-2 was decreased, whereas p53 showed a great increase following JTE-522 treatment. Their changes were in a dose-dependent manner. CONCLUSION: These findings suggest that reactive oxygen species, NF-kB, p53, bcl-2 and caspase-3 may play an important role in the induction of apoptosis in AGS cells after treatment with JTE-522.

关键词： I-kappa B Proteins Adenocarcinoma Apoptosis Benzenesulfonates Caspases Cell Division DNA-Binding Proteins Humans NF-kappa B Oxazoles Proto-Oncogene Proteins c-bcl-2 Reactive Oxygen Species Research Support, Non-U.S. Gov't Stomach Neoplasms Tumor Cells, Cultured Tumor Suppressor Protein p53

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A problem of complex oscillation for homogeneous linear differential equations

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Chinese Science Bulletin 1998年第20期43卷 1709-1712页

作者： GAO Shian and YANG Ronghua department of Mathematics,South china Normal university,guangzhou 510631, china department of Mathematics, guangdong Educational college, guangzhou 510303, china department of Mathematics South China Normal University Guangzhou China department of Mathematics Guangdong Educational College Guangzhou China

Under a combined dominant condition, an open problem of complex oscillation for the equation \%w (k) +Aw=0\% is set, where \%k≥3, a(z)\% is a transcendental entire function.

关键词： meromorphic function differential equation complex oscillation.

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Reply to“Comment on ‘Recent progress in thermodynamics of radiation—Exergy of radiation,effective temperature of photon and entropy constant of photon'”

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Science china(Technological Sciences) 2010年第3期53卷 878-880页

作者： CHEN ZeShao 1,MO SongPing 1,2 &HU Peng 1 1department of Thermal Science and Energy Engineering,university of Science and Technology of china,Hefei 230027,china 2 Faculty of Material and Energy,guangdong university of Technology,guangzhou 510006,china department of Thermal Science and Energy Engineering University of Science and Technology of China Hefei China Faculty of Material and Energy Guangdong University of Technology Guangzhou China

Two counterexamples in Liu's comment on'Recent Progress in Thermodynamics of Radiation—Exergy of radiation,effective temperature of photon and entropy constant of photon'are analyzed and the incorrect applications of the related concepts are *** similarities and differences between the concepts of the effective temperature of photon,the exergy of spectral radiation,the entropy constant of photon and the macroscopic concepts of temperature,exergy,and entropy are further *** duality of radiant energy,micro-state quantum characteristic and macro-state thermal characteristic is presented.

关键词： thermodynamics of radiation photon entropy

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