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中国生理学会心血管生理学术研讨会

作者： Qian Li,Biying Sun and Weifang Rong department of physiology,shanghai jiaotong university School of medicine,shanghai,china

We and others previously reported experimental evidence suggesting an important role of hydrogen sulfide(HS) in oxygen sensing in murine chemoreceptors and in blood vessels. However,the hypothesis of HS mediated oxygen sensing has been challenged recently by several *** particular,it was shown that exogenous HS inhibited the release of neurotransmitters(ACh,ATP) from the isolated cat carotid body,raising the possibility that HS signaling varies across *** therefore set out to determine the effect of HS on carotid body function in different *** data indicate that in mice,rats and rabbits, application of HS donors(NaHS or NaS) evoked consistent activation of the afferent nerves of the isolated carotid body(sinus nerve) that was prevented by the presence of calcium channel blocker cadmium(100μM).The excitatory effects of HS were concentration-dependent and *** the rabbit carotid body,HS-mediated excitation of sinus nerve was usually followed by a brief period of inhibition,suggestive of multiple signaling pathways triggered by *** consider HS-mediated oxygen-sensing is common across species and that the current discrepancies regarding the effects of exogenous HS in the carotid body may be attributable to the activation of multiple and variant molecular targets in different species.

关键词： hydrogen sulfide carotid body oxygen-sensing species difference

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Effects of U-50 488H a k-agonist on action potentials of isolated ventricular papillary muscle of guinea pigs

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Acta Pharmacologica Sinica 1994年第1期 37-39页

作者： SHENG Jian-Zhong, DONG Hui, WONG Tak-Ming(department of physiology, Faculty oj medicine, The university oj Hong Kong, Hong Kong) Recipient of a fellowship from Sun Yat Sen Foundation, now in department of physiology, Zhejiang Meidical university, Hangzhou 310006, china.department of Biophysics, School of basic Medical Sciences, shanghai Medical university, shanghai 200032, china.

Trans (+-)-3,4-dichloro-N-methyl-N-[ 2-( 1-pyrrolidinyl ) cyclohexyl ]-benzeneacetamide-methanesulfonate-hydrate (U-50 488H), a specific K-agonist. at 1 - 10 μmol·L-1 caused concentration-dependent reductions in the action potential duration at 5.0% and 90% of repolarization (APD50 and APD90) without modifying the resting potential (RP), the action potential amplitude (APA) and the maximal upstroke velocity (Vmax). The effects were attenuated by (- )- (lR,5R.9R)-5,9-diethyl-2-(3-furylmethyI)-2 L - hydroxy- 6 , 7 - benzomorphan (Mr 2266 BS, 1 μmol·L-1), a specific K-antagonist which itself had no effect on the action potentials of the ventricular papillary muscle of guinea pigs, indicating that U-50 -188H at 1-10 μmol·L-1 acts via specific cardiac K-receptors. At 100 μmol·L-1, U-50 488H not only shortened APD50 and APD90 but also reduced RP, APA, and Vmax which were not attenuated by Mr 2266 BS (1 μmol·L-1) suggesting that the effects of U-50 488H at 100 μmol · L-1 were probably nonspecific.

关键词： U-50 488H Mr 2266 BS endorphin receptors action potentials papillary muscles guinea pigs

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Curcumin attenuates gastrointestinal afferent nerve sensitivity via antagonizing TRPV1

Curcumin attenuates gastrointestinal afferent nerve sensitiv...

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第九届全国钙信号和细胞功能研讨会

作者： Leilei Zhi,Decun Kong,Biying Sun,Qiao Sun,Guohua Zhang,Weifang Rong department of physiology,shanghai jiaotong university School of medicine,shanghai 200025, china

＜正＞Background and purpose:An antinociceptive effect has been shown for curcumin in animal models and in humans,but the molecular mechanisms of curcumin’s effect remain poorly *** the present study,we explored the possibility that curcumin inhibit visceral sensitivity via antagonizing the transient receptor potential vanilloid-1 （TRPV1）receptor. Experimental approach:The effects of curcumin on colorectal distension(CRD,0-

关键词： curcumin TRPV1 visceral sensitivity capsaicin functional gastrointestinal diseases

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Group Ⅰ Metabotropic Glutamate Receptors Contribute to Visceral Nociception

Group Ⅰ Metabotropic Glutamate Receptors Contribute to Visc...

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2006中国科协年会

作者： Hu Youmin, Li Dong, Biying Sun, Qian Li, Xiaofang Wang, Weibing Shi and Weifang Rong department of physiology, shanghai jiaotong university School of medicine, shanghai 200025, china

Metabotropic glutamate receptors (mGluRs1-8) are members of the G protein-coupled receptor (GPCR) superfamily and they have been implicated in processes requiring synaptic plasticity such as learning and memory, neuronal development and neurodegeneration. mGluR5,a member of the group I mGluRs,is distributed throughout the central nervous system including areas involved in nociception and in unmyelinated sensory afferent terminals as well. Pilot observations in the rat showed that the mGluR5 antagonist,MPEP, significantly raised the voiding threshold. The present study aims to delineate the role of mGluR5 in visceral afferent visceral nociception. In the isolated mouse bladder/pel-

关键词： mGluR,MPEP,visceral nociception, urinary bladder

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Hydrogen sulfide promotes angiogenesis in vitro and in vivo

Hydrogen sulfide promotes angiogenesis in vitro and in vivo

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Ming-Jie WANG~1,Wen-Jie CAI~2,Dan-Dan HAO~1,Na LI~1,Ying CHEN~1,Ying-Jiong DING~1,Yi-Chun ZHU~1 1 department of physiology and Pathophysiology,Fudan university shanghai Medical college.shanghai 200032,china 2 department of basic medicine,university of shanghai for Science and Technology,shanghai 200093,china

Introduction:Hydrogen sulfide(HS),a novel gasotransmitter,has been shown to play an important role in regulating cardiovascular functions,including maintaining basal systolic blood pressure,inhibiting neointimal hyperplasia,exerting negative inotropic effect and inhibiting ischemia-reperfusion *** date,there is little information about the potential role of HS in ***:Cultured RF/6A endothelial cells,C57 mouse Matrigel plug model and rat hind limb ischemia model were *** vitro angiogenesis study includes endothelial cell proliferation,migration and tube formation assay,and ERK,Akt and p38 signaling *** measurement and morphological analysis were performed with ex vivo Matrigel plug *** vivo angiogenesis study includes microangioraphy,fluorescent microsphere assay,capillary density quantification and VEGF-VEGFR2 expression ***:10μmol/L NaHS(HS donor) promoted RF/6A endothelial cell proliferation,migration and tube *** angiogenic response is largely dependent on activation of PI3K/Akt.10 and 50μmol/kg/d NaHS also promoted ex vivo angiogenesis in C57 mouse Matrigel plug model.50μmol/kg/d NaHS significantly increased collateral vessel formation,capillary density and blood supply to the ischemic hind *** upregulation of VEGF expression in ischemic skeletal muscles and VEGFR2 phosphorylation in neighboring vascular ECs suggest a role for VEGF in mediating the effects of NaHS on interactions between myocytes and endothelial cells. Conclusions:Exogenous HS is a pro-angiogenic factor both in vitro and in *** may therefore be used as a supplement to ischemic diseases.

关键词： hydrogen sulfide angiogenesis endothelial cells hind limb ischemia

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Anatomy and physiology of peripheral auditory system and commen causes of hearing loss

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Journal of Otology 2009年第1期4卷 -页

作者： ZHANG Qing1,DUAN Mao-li1-4 1 department of Otorhinolarygology,The 2nd Affiliated Hospital of Xi’an jiaotong university,School of medicine,Xi’an jiaotong university,Xi’an,P.R.china 2 department of clinical neuroscience,3,department of Otolaryngology,Karolinska Hospital,171 76 Stockholm,Sweden 4 department of Otolaryngology,Affiliated Hospital of Anhui Medical university,china department of Otorhinolarygology The 2nd Affiliated Hospital of Xi’ an Jiaotong UniversitySchool of Medicine Xi’ an Jiaotong University Xi’ an P.R.China department of clinical neuroscience Karolinska Hospital 171 76 Stockholm Sweden department of Otolaryngology Karolinska Hospital 171 76 Stockholm Sweden department of Otolaryngology Affiliated Hospital of Anhui Medical University China

For Otolaryngologist,it is the most important to know the principle of anatomy,physiology and common *** but more concise summary has been sum up in the following review in order to help young ENT doctor to understand... 详细信息

关键词： inner hair cell outer hair cell spiral ganglion neuron ototoxicity

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The study of bone tissue and vessels constructed by bidirectional induced bone marrow mesenchymal stem cells of rabbits in vitro

The study of bone tissue and vessels constructed by bidirect...

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2011年第十一届上海地区医用生物材料研讨会——生物材料与再生医学进展

作者： Guo Shangchun~1,Chen Xin~2,Wang Jinwu~(3*),Rui Biyu~2,Sun Hui~2,Dai Kerong~3 I.shanghai Institue for Microsurgery of Extermities,The Sixth People’s Hospital Affiliated to shanghai jiaotong university,shanghai 200233,china 2.department of Orthopedics,The Sixth People’s Hospital Affiliated to shanghai jiaotong university,shanghai 200233,china 3.department of Orthopedics,The ninth People’s Hospital Affiliated to shanghai jiaotong university School of medicine,shanghai 200011,china

Objective To study the ability of construction of bone tissue and vessels in vitro by the co-culture system including endothelial cells,osteoblasts and bone marrow Mesenchymal stem cells(BMSCs) from BMSCs bidirectional *** BMSCs were isolated from bone marrow by density gradient centrifugation. The adhesive cells were preserved to passage in *** cells were divided into four groups:group A(pure

关键词： Bone marrow mesenchymal stem cells Co -culture system In vitro angiogenesis assay Bidirectional induce

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Understanding structure and function of acid-sensing ion channels

Understanding structure and function of acid-sensing ion cha...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Tian-Le XU~(1,2) 1 Institute of Neuroscience and State Key Laboratory of Neuroscience,shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,shanghai 200031,china 2 Neuroscience Division,department of Biochemistry and Molecular Cell Biology,Institute of Medical Sciences, shanghai jiaotong university School of medicine,shanghai 200025,china

The acid-sensing ion channel(ASIC) has a prominent role in tissue pH-sensing and neuronal signalling. Subunits of ASICs are members of the degenerin/epithelial Na channel protein *** date,seven ASIC subunits have been identified:ASIC1a,ASIC1b,ASIC1b2,ASIC2a,ASIC2b,ASIC3,and *** subunit contains cytosolic N and C termini,two alpha-helical transmembrane domains(M1-M2),and a disulfide-rich multidomain extracellular *** form a variety of homo- and heterotrimeric ion channels that are activated by extracellular acidosis associated with neurotransmission,injury,inflammation and ischemia,triggering membrane depolarization via Na influx into target *** the operating mechanisms of these ion channels could therefore provide the catalyst for further studying ASIC physiology and pharmacology,as well as promoting the development of experimental techniques for other families of ion channels and/or *** this talk,I will present our current work on ASIC structure and function,in particular those of ASIC1a and ASIC3 *** in this area has been stimulated in recent years by the publication of high-resolution structures of ASIC1,which have permitted the structure-directed approaches and the creation of ASIC homology models.I will first discuss the crucial roles of ASICs in mediating neuronal injury and pain *** a series of computational,chemical and functional approaches,and nonproton ligands(GMQ,agmatine and amiloride) as probes,I will then delineate the nonproton ligand sensing domain in ASIC3 channels at the atomic level and describe how chemical biology,homology modeling and ligand docking can provide useful information about ligand interactions.I will also consider some of the ongoing projects in my laboratory that are investigating the pathophysiological roles of ASICs using ASIC subunit gene knockout *** report ends with a discussion on the generality of ASICs as a model system for the study of struc

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Actin microfilament involved in regulation of pacemaking activity increased by membrane stretch in cultured ICCs from murine intestine

Actin microfilament involved in regulation of pacemaking act...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Zuo-Yu WANG,Yan-Fei HAN,Xu HUANG,Hong-Li LU,Xin GUO,Wen-Xie XU department of physiology,shanghai jiaotong university School of medicine shanghai,200240,china

Distension is a regular mechanical stimulus in gastrointestinal(GI) *** study is designed to investigate the effect of membrane stretch on pacemaking activity and whether actin microfilament is involved in its mechanism in cultured murine intestinal interstitial cells of Cajal(ICCs) by using whole-cell patch-clamp and calcium imaging *** stretch induced sustained inward holding current from the baseline to(647.38±105.07) pA and significantly decreased amplitudes of pacemaker current from(222.25±51.76) pA to(141.17±46.45) pA(n=6,P20μmol/L cytochalasin-B,a disruptor of actin microfilaments,significantly suppressed amplitude of pacemaker currents and calcium oscillations from(491.32±160.33) pA and(0.30±0.06)(ΔF/F0) to(233.12±92.00) pA and(0.09±0.02) (ΔF/F0)(n=6,P20μmol/L phalloidin,a stabilizer of actin microfilaments,significantly enhanced amplitude of pacemaker currents and calcium oscillations from(751.79±282.82) pA and(0.56±0.13)(ΔF/F0) to (1234.34±607.83) pA and(0.72±0.08)(ΔF/F0)(n=6,P2±0.07) when pretreatment with *** results suggest that membrane stretch potentiates pacemaking activity via actin microfilaments and the process is mediated by altering intracellular basal calcium concentration and calcium oscillation in cultured intestinal ICCs.

关键词： ICCs membrane stretch actin microfilament pacemaking activity calcium oscillation.

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CLONING PROMOTER OF HUMAN SATBl GENE AND EFFECT OF ATRA AND CoCl_2 ON ITS ACTIVITY

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Journal of shanghai Second Medical university(Foreign Language Edition) 2006年第1期18卷 1-6页

作者：李颖戴冰冰蔡蓉仇全于丽莉卢健 department of Biochemistry & Molecular Biology

Objective To explore the structure and activity of SATB1 promoter in different cells, ATRA and CoCl2 effect on its activity. Methods Using luciferase system to assay the promoter activity of human SATB1 gene, three luciferase reporter vectors were constructed which driven by different regions of 5＇ untranslated sequence from human SATB1 gene , called pGL3-SP2946-luc , pGL3-SP1718-luc and pGL3-SP751-luc , and transfected into Jurkat T, K562, U937 and Hela cells transiently using lipofectinamine, the expression activity was detected at different dosage of A TRA and CoCl2treatment for different time course. Results The reporter gene expression from SATB1 promoter were high activity in U937 cell, moderate in Jurkat T cell, low activity in K562 cell and showed no obvious activity in Hela cell, the reporter gene expression from pGL3-SP751-1uc kept on the higher lever in Jurkat T, K562 and U937 cells than the other two vectors. We also found that the repressive effect of CoCI2 on SATBI＇s mRNA expression and the relative luciferase expression from pGL3-SP751-luc in U937 cell was down-regu- lated obviously by ATRA and CoCI2 in the concentration- and time-dependent manners. Conclusion SATB1 pro- rooter drives gene expression with cell-specificity and its core promoter region maybe exist in the - 751 - - 9bp of 5＇ untranslated region of human SATB1 gene. Combined with the experiment result we found before that SATB1 was down-regulated by ATRA in U937, the results imply that STAB1 maybe is down-regulated by ATRA and CoCl2 through its promoter in the differentiation of myeloid cell line-U937.

关键词： SATB1 promoter ATRA CoCl2

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