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Atomic Force Microscopy-based Cell Nanostructure for Ligand-conjugated Quantum Dot Endocytosis

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 646-652页

作者： Yun-Long PAN~1* Ji-Ye CAI~2 Li QIN~3 Hao WANG~4 1 The First Affiliated Hospital of jinan university,guangzhou 510632,china 2 college of life science and technology,jinan university,guangzhou 510632,china 3 Department of Histology and Embryology,Medical college of jinan university,guangzhou 510632,china 4 Information and Optoelectronic science and Engineering.South china Normal university,guangzhou 510631,china The First Affiliated Hospital of jinan universityguangzhou 510632 China college of life science and technology Jinan UniversityGuangzhou 510632 China Department of Histology and Embryology Medical College of Jinan UniversityGuangzhou 510632 China School for Information and Optoelectronic science and Engineering South China Normal UniversityGuangzhou 510631 China

While it has been well demonstrated that quantum dots (QDs) play an important role inbiological labeling both in vitro and in vivo,there is no report describing the cellular nanostructure basis ofreceptor-mediated ***,nanostructure evolution responses to the endocytosis of transferrin(Tf)-conjugated QDs were characterized by atomic force microscopy (AFM).AFM-based nanostructureanalysis demonstrated that the Tf-conjugated QDs were specifically and tightly bound to the cell receptorsand the nanostructure evolution is highly correlated with the cell membrane receptor-mediated ***,confocal microscopic and flow cytometry results have demonstrated the specificity anddynamic property of Tf-QD binding and *** found that the internalization of Tf-QD is linearlyrelated to ***,while the nanoparticles on the cell membrane increased,the endocytosis was stillvery active,suggesting that QD nanoparticles did not interfere sterically with the binding and function ***,ligand-conjugated QDs are potentially useful in biological labeling of cells at a nanometerscale.

关键词： quantum dot endocytosis nanostructure atomic force microscopy

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Generation and Characterization of C305,a Murine Neutralizing scFv Antibody That Can Inhibit BLyS Binding to Its Receptor BCMA

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 415-420页

作者： Mei-Yun LIU~(1.2) Wei HAN~(2.3) Yan-Li DING~(2.3) Tian-Hong ZHOU~1 Rui-Yang TIAN~2 Sheng-Li YANG~2 Hui LIU~2 Yi GONG~2 1 college of life science and technology,jinan university. guangzhou 510632, china 2 Shanghai Research Center of Biotechnology.Shanghai Institutes for Biological sciences. Chinese Academy of sciences. Shanghai 200233,china 3 School of Pharmaceatical Engineering, Shenyang Pharmaceutical university. Shenyang 110016. china college of life science and technology Jinan UniversityGuangzhou 510632 China Shanghai Research Center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200233 China School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016 China

B-lymphocyte stimulator(BLyS)is a member of the tumor necrosis factor(TNF)family anda key regulator of B cell *** single-chain fragment variable(scFv)antibody against BLySbinding to its receptor BCMA has the potential to play a prominent role in autoimmune disease *** display scFv library constructed on pⅢ protein of M13 filamentous phage was screened using *** five rounds of panning,their binding activity was characterized by *** sequencingrevealed that at least two different scFv gene fragments(C305 and D416)were *** two differentscFv gene fragments were expressed to obtain the soluble scFv antibodies,then the soluble scFv antibodieswere characterized by means of competitive ELISA and in vitro neutralization *** results indicatedthat C305 is the neutralizing scFv antibody that can inhibit BLyS binding to its receptor BCMA.

关键词： phage display neutralizing scFv antibody BLyS

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日本鳗鲡脂肪酸去饱和酶和延长酶基因的克隆与分析

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水生态学杂志 2009年第5期2卷 56-62页

作者：郁颖梁旭方李观贵何珊谢骏白俊杰暨南大学生命科学技术学院广东广州510632 中国水产科学研究院珠江水产研究所广东广州510380

利用RT-PCR和RACE方法克隆得到日本鳗鲡(Anguillaja ponica)肝脏中控制高不饱和脂肪酸(highly unsat-urated fatty acids,HUFA)合成的脂肪酸去饱和酶(fatty acid desaturase,FAD)和脂肪酸延长酶(fatty acid elongase,ELO)全长cDNA序列。结果表明,2456bp的FAD全长cDNA序列含长达1046bp的3′-UTR、75bp的5′-UTR和编码444个氨基酸的长1335bp的阅读框。编码的蛋白序列含有FAD全部的特征结构区,包括3个组氨酸簇、2个跨膜区和1个细胞色素b5结构域,与其他具有不同生活史鱼类的FAD氨基酸序列具有70.5%～77.5%的同源性;分析显示其在系统树中与溯河洄游鱼类的亲缘关系最近。克隆得到的ELO全长cDNA序列长1239bp,含有885bp的开放阅读框,编码294个氨基酸,该蛋白序列含有单一的氧化还原中心组氨酸簇、内质网停留信号和多个跨膜区等ELO特征结构,与其他鱼类ELO氨基酸具有87.1%～88.8%的同源性;其在系统树中与北非鲶鱼(Clarias gariepinus)和斑马鱼亲缘关系较近。日本鳗鲡FAD和ELOcDNA全序列的获得为今后进一步研究其体内高不饱和脂肪酸合成途径及阐明该途径在不同鱼类中的分子进化机理奠定基础。

关键词：脂肪酸去饱和酶脂肪酸延长酶基因克隆日本鳗鲡

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Interaction of Mouse Pem Protein and Cell Division Cycle 37 Homolog

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 784-787页

作者： Fen GUO Yue-Qin LI Shi-Qian LI Zhi-Wen LUO Xin ZHANG Dong-Sheng TANG Tian-Hong ZHOU college of life science and technology,jinan university.guangzhou 510632.china college of life science and technology Jinan UniversityGuangzhou 510632 China

Mouse Pem,a homeobox gene,encodes a protein consisting of 210 amino acid *** the function of mouse Pem protein,we used the yeast two-hybrid system to screen the library of 7-daymouse embryo with full-length mouse Peru ***-two colonies were obtained after 1.57×10~8 colonieswere screened by nutrition limitation and β-galactosidase *** individual insert fragments wereobtained from the library,and three of them were identified,one of which was confirmed to be the celldivision cycle 37(Cdc37)homolog gene by *** interaction between mouse Pem and Cdc37homolog was then confirmed by glutathione S-transferase pull-down assay,and the possible interaction modelwas suggested.

关键词： mPem cell division cycle 37 homolog yeast two-hybrid system

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Diversity, distribution, and new phylogenetic information of calcareous dinoflagellates from the china Sea

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Journal of Systematics and Evolution 2011年第2期49卷 126-137页

作者： 1 Hai-Feng GU 1 Zhao-He LUO 2 Yan WANG 1 Dong-Zhao LAN 1 (Third Institute of Oceanography, Xiamen 361005, china) 2 (Research Center for Harmful Algae and Aquatic Environment, college of life science and technology, jinan university, guangzhou 510632, china) (Third Institute of Oceanography Xiamen 361005 China) (Research Center for Harmful Algae and Aquatic Environment College of Life Science and Technology Jinan University Guangzhou 510632 China)

Calcareous dinoflagellates have been recorded from subarctic to tropical areas, inhabiting both neritic and oceanic regions. In the present study we analyse 12 sediment samples from the china Sea to establish the existence of calcareous dinoflagellates in this area. A total of 11 calcareous dinoflagellate species were recorded, among them, three genera (Calciodinellum, Leonella, Posoniella) and seven species (Calciodinellum albatrosianum, C. levantinum, C. operosum, Leonella granifera, Posoniella tricarinelloides, Scrippsiella irregularis, and S. regalis) were reported from the china Sea for the first time. Cysts of S. irregularis and S. precaria are identical, with a mesoepicystal archeopyle, representing the loss of 2–4 and 1–3a paraplates; however, their thecal plates vary slightly and differ from each other in 78 positions of the internal transcribed spacer sequence. Scrippsiella trochoidea is dominant in coastal areas, whereas Calciodinellum albatrosianum is the most abundant offshore. Scrippsiella irregularis is distributed widely along the coast of china, whereas S. precaria is recorded only in northern china. Leonella granifera is the second most abundant species offshore, comprising 15–40% of the cyst assemblage.

关键词： calcareous dinoflagellates Calciodinellum china Sea Leonella Posoniella Scrippsiella.

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In Vitro Construction of Effective M1GS Ribozymes Targeting HCMV UL54 RNA Segments

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 210-214页

作者： Yun-Zhen SU Hong-Jian LI Yue-Qin LI Hao-Jun CHEN Dong-Sheng TANG Xin ZHANG Hong JIANG Tian-Hong ZHOU college of life science and technology,jinan university,guangzhou 510632,china college of life science and technology Jinan UniversityGuangzhou 510632 China

Seven sequence-specific ribozymes(M1GS RNAs)derived in vitro from the catalytic RNAsubunit of Escherichia coli RNase P and targeting the mRNAs transcribed by the UL54 gene encoding theDNA polymerase of human cytomegalovirus were screened from 11 ribozymes that were designed based onfour rules:(1)the NCCA-3′ terminal must be unpaired with the substrate;(2)the guide sequence(GS)mustbe at least 12 nt in length;(3)the eighth nucleotide must be U,counting from the site-1;and(4)around thecleavage site,the sites-1/+1/+2 must be U/G/C or C/G/*** investigation of the factors affecting thecleavage effect and the optimal ratio for M1GS/substrate was carried *** was determined that the optimalratio for M1GS/substrate was 2:1 and too much M1GS 1ed to substrate *** indicated *** M1GS that cleaved HCMV UL54 RNA segments in vitro were successfully designed and *** studies support the use of ribozyme M1GS as antisense molecules to silence HCMV mRNA in vitro,andusing the selection procedure as a general approach for the engineering of RNase P ribozymes.

关键词： ribonuclease P (RNase P) guide sequence HCMV DNA polymerase

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Microwave peak absorption frequency of liquid

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science china(Physics,Mechanics & Astronomy) 2008年第9期51卷 1254-1263页

作者： HAN GuangZe1 & CHEN MingDong2 1 Department of Physics,South china university of technology,guangzhou 510640,china 2 guangzhou Auto college,South china university of technology,guangzhou 510800,china Department of Physics South China University of Technology Guangzhou China guangzhou Auto college South China University of Technology Guangzhou China

Microwave-assisted extraction is a new effective method which has practical ap-plications in many fields. Microwave heating is one of its physical mechanisms,and it also has the characteristic of selectivity. When the applied microwave fre-quency equals a certain absorption frequency of the material (or specific compo-nent),the material will intensively absorb microwave energy. This is also known as resonant absorption,and the frequency is called the peak absorption frequency which depends on the physical structure of the material. In this work,dynamic hy-drogen bond energy was included in molecular activation energy; with the liquid cell model,the expression of interaction energy between dipolar molecules was derived. The rotational relaxation time was gotten from the Eyring viscosity formula. Then based on the relationship between dielectric dissipation coefficient and re-laxation time,the expression of microwave peak absorption frequency as a func-tion of the material physical structure,rotational inertia and electrical dipole mo-ment of molecules was established. These theoretical formulas were applied to water and benzene,and the calculated results agree fairly well with the experi-mental data. This work can not only deepen the study of the interaction between microwave and material,but also provide a possible guide for the experiment of microwave-assisted extraction.

关键词： microwave-assisted extraction microwave heating relaxation time liquid viscosity peak absorption fre-quency

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Investigation on the Surface Coating of Grinding Balls

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Rare Metals 2001年第4期20卷 259-264页

作者： Chengguo Wang1 Yujun Bai1.2 1) college of Materials science and Engineering, Shandong university, jinan 250061, china 2) Mechanical Department. Shandong university of science and technology. jinan 25003), china 山东大学山东济南 250061

The surface coating of grinding balls was investigated experimentally. The results show that a coating may form on the surface of grinding balls when Cr or Al powders are subjected to ball grinding. The plastic deformation of the ball surface plays an important role during the coating formation, and the strong binding force between the powders and the balls is a necessary pre-condition. The thickness of coating increases with the plasticity of the powders and the balls. Annealing the balls with coating will result in an obvious diffusion of the elements in the bonding zone of interface.

关键词： coating ball grinding plastic deformation

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Phylogeny of Ptychostomum (Bryaceae,Musci) inferred from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) and chloroplast rps4

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Journal of Systematics and Evolution 2009年第4期47卷 311-320页

作者： ~(1,2)Chen-Ying WANG ~1Jian-Cheng ZHAO~* 1(college of life science,Hebei Normal university,Shijiazhuang 050016,china) 2(Department of life science,Zhengzhou Teachers college,Zhengzhou 450044,china) (college of life science Hebei Normal University Shijiazhuang 050016 China) (Department of life science Zhengzhou Teachers College Zhengzhou 450044 China)

The phylogeny of Ptychostomum was first undertaken based on analysis of the internal transcribed spacer(ITS)region of the nuclear ribosomal(nr)DNA and by combining data from nrDNA ITS and chloroplast DNA rps4 *** parsimony,maximum likelihood,and Bayesian analyses all support the conclusion that the reinstated genus Ptychostomum is not *** funkii(Schw(a|¨)gr.)*** (≡Bryum funkii Schw(a|¨)gr.)is placed within a clade containing the type species of Bryum,*** Hedw. The remaining members of Ptychostomum investigated in the present study constitute another well-supported clade. The results are congruent with previous molecular *** the basis of phylogenetic evidence,we agree with transferring *** Mü***.(≡***(Brid.)Turton≡Bryum archangelicum Bruch & Schimp.), Bryum lonchocaulon Mü***.,Bryum pallescens *** Schw(a|¨)gr.,and Bryum pallens *** Ptychostomum.

关键词： Bryum molecular phylogeny nuclear ribosomal DNA internal transcribed spacer sequences Ptychostomum rps4 sequences.

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Effects of temperature and organic and inorganic nutrients on the growth of Chattonella marina (Raphidophyceae)from the Daya Bay,South china Sea

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Acta Oceanologica Sinica 2011年第3期30卷 124-131页

作者： WANG Zhaohui YUAN Meiling LIANG Yu LU Sollghui college of life science and technology Jinan University Guangzhou 510632 China Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education Guangzhou 510632 China

The effects of temperature and different forms of nutrients on Chattonella marina growth have been investigated in strains isolated from the Daya Bay, the South china Sea. The strain of C. marina preferred high temperatures, with an optimal temperature of 25℃, and 18℃ was the minimum for its survival. Higher cell number and growth rate were obtained in high nitrogen and phosphorus concentrations （500 μg/L, 74μg/L） than under nutrient limitation. Nitrogen influenced the growth most, as the specific growth rate and maximum cell density were lower in nitrogen- limited cultures than noted under phosphorus limitation or under limitation from both. C. marina was capable of using many kinds of organic nitrogen sources including L-serine （L-Ser）, glycine （Gly）, alanine （Ala）, L-threonine （L-Thr）, glutamic acid （Glu） and urea, but could not utilize uric acid. Various forms of organic phosphorus compound such as glucose-6-phosphate （G6P）, sodium glycerophosphate （GYP）, adenosine triphosphate （ATP）, adenosine monophosphate （AMP）, cyti- dine monophosphate （CMP）, guanosine monophosphate （GMP）, uridine monophosphate （UMP）, 4-nitrophenylphosphate （NPP） and triethyl phosphate （TEP） supported the growth as well. Algal cells had the ability to sustain growth under nitrogenand/or phosphorus-free conditions particularly under phosphorus depleted condition. These results led to the hypothesis that high loading of nitrogen has played an important role in frequent C. marina blooms in the past decade, and its capability for utilization of diverse forms of organic nutrients and growth in low nutrient conditions make this species a likely recurrent dominant in the Daya Bay phytoplankton assemblages, visible as more frequent blooms.

关键词： Chattonella marina growth inorganic and organic nutrients salinity temperature

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