检索结果-南通市图书馆

Differentially Expression of Tual,a Tubulin-encoding Gene,during Flowering of Tea Plant Camellia sinensis (L.) *** Using cDNA Amplified Fragment Length Polymorphism Technique

引用

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 653-662页

作者： Wan-Ping FANG~(1,2) Chang-Jun JIANG~1* Mei YU~1 Ai-Hua YE~1 Zhao-Xia WANG~(1,3) 1 Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui agricultural university,Hefei 230036,china.2 college Of horticulture,Nanjing Agriculture university,Nanjing 210095,china.3 Auhui Institute of Education,Hefei 230036,china Key Laboratory of Tea Biochemistry and Biotechnology Ministry of Agriculture Anhui Agricultural UniversityHefei 230036 China college Of horticulture Nanjing Agriculture UniversityNanjing 210095 China Auhui Institute of EducationHefei 230036 China

The complementary DNA （cDNA） amplified fragment length polymorphism technique wasused to isolate transcript-derived fragments corresponding to genes involved in the flowering of tea *** sequence analysis of an approximately 300 bp differential fragment amplified by primer combi-nation E11M11revealed 80%-84% similarity to the corresponding part of an α-tubulin gene of other *** complete cDNA sequence of this α-tubulin was cloned by the rapid amplification of cDNA ends technique;its full length is 1537 bp and contains an open reading frame of 450 amino acid residues with two N-glycosylation sites and four protein kinase C phosphorylation *** deduced amino acid sequences didshow significant homology to the α-tubulin from other plants that has been reported to be a pollen-specificprotein and could be correlated with plant cytoplasm-nucleus-interacted male *** named this completecDNA *** nucleotide and amino acid sequence data of Tual have been recorded in the GenBanksequence database with the accession *** Tual gene was cloned into the pET-32aexpression system and expressed in Escherichia coli BL21trxB（DE3）.The molecular weight of expressedprotein was deduced to be approximately 49 *** blot analysis was used to identify the temporalexpression of Tual in tea *** studies of the effect of Tual protein on pollen tube growth indicatedthe Tual solution obviously promoted the growth of tea pollen tube.

关键词： cDNA-AFLP Camellia sinensis flower bud α-tubulin Western blot pollen

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Characterization of Nocardia Plasmid pXT107

引用

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 620-624页

作者： Hai-Yang XIA~(1,2#) Yong-Qiang TIAN~(2#) Ran ZHANG~2 Kai-Chun LIN~1* Zhong-Jun QIN~2* 1 college of Plant Science and Technology huazhong agricultural university,wuhan 430070,china.2 Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,china college of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200032 China

Nocardia,Rhodococcus and Streptomyces,all members of the actinomycetes family,areGram-positive eubacteria with high G+C content and able to form *** report here a newlyidentified plasmid pXT107 of Nocardia sp.107,one of the smallest circular plasmids found in *** complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G+C content,and encodedone replication extragenic palindromic (Rep) and six hypothetical *** Rep,double-strand originand single-strand origin of pXT 107 resembled those of typical rolling-circle-replication plasmids,such aspNI100 of Nocardia,pRE8424 of Rhodococcus and pIJ101 of *** Escherichia coli-Nocardiashuttle plasmid pHAQ22,containing the rep gene of pXT107,is able to propagate in Nocardia but not inStreptomyces.

关键词： Nocardia plasmid complete nucleotide sequence replication

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Palynological characters of Glycyrrhiza,Glycyrrhizopsis,and Meristotropis(Leguminosae),with special reference to their taxonomic significance

引用

Journal of Systematics and Evolution 2010年第6期48卷 455-463页

作者： 1,2Lei MENG 2Xiang-Yun ZHU 1(college of Biological Sciences,china.agricultural university,Beijing 100094,china. 2(Institute of Botany,Chinese Academy of Sciences,Beijing 100093,china. (college of Biological Sciences China Agricultural University Beijing 100094 China) (Institute of Botany Chinese Academy of Sciences Beijing 100093 China)

The pollen morphology of 11 species of the genus Glycyrrhiza *** one from each of the genera Glycyrrhizopsis Boiss.& *** Meristotropis Fisch.& *** investigated by scanning electron *** pollen morphology,the main differences between Glycyrrhizopsis and Glycyrrhiza are:Glycyrrhizopsis-pollen grains 36.63 × 40.42 μm in size,oblate spheroidal in shape;and Glycyrrhiza-pollen grains 24.47-33.18 × 23.82-31.83 μm in size,prolate spheroidal in *** and Glycyrrhiza should be recognized as two distinct genera based on palynological and morphological *** and Glycyrrhiza are similar in many important palynological and morphological characters,suggesting that the two should be *** Glycyrrhiza,two types of pollen grains,3-lobed-circular or subtriangular in polar view,are found in different species,in accordance with morphological differences in the two groups,shedding light on the classification and evolution of the genus.

关键词： exine sculpture morphological character pollen morphology taxonomic revision.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Ability of Agrogyron elongatum to accumulate the single metal of cadmium, copper, nickel and lead and root exudation of organic acids

引用

Journal of Environmental Sciences 2001年第3期13卷 368-375页

作者： YANG Hong 1, Jongathan W.C.Wong 2, YANG Zhi min\+1, ZHOU Li xiang 1(1. college of Science, Nanjing agricultural university, Nanjing\ 210095, china. 2. Department of Biology, Hong Kong Baptist university, china. 南京农业大学江苏南京 210095 香港浸会大学香港

Agrogyron elongatum were grown in nutrient solution containing moderate to high amounts of separate heavy metal of Cd, Cu, Ni and Ph in a greenhouse for a 9 - day. Cd, Cu, Ni and Ph generally led to decrease in the elongation of roots although the length of seedlings exposed to Cd and Ph at 0.05 and 0.5 mg/L showed to be slightly greater than that of controls. Of the four metals in the experiment, Ph was absorbed and accumulated to the highest level, with the concentrations of 92754 mg/kg dry weight (DW) in roots and 11683 mg/kg DW in shoots. Cd was moderately accumulated in Agrogyron elongatum, but the maximum bioaccumulation coefficients (BCs) for roots and shoots were observed. The patterns for Cu and Ni uptake and distribution in plants differed from those of Ph and Cd, as it was showed that the shoot accumulation of Cu and Ni was significantly higher than in roots. A. elongation had the highest Ni concentration in shoots (30261 mg/kg DW) at the external concentration of 250 mg/L. Cu ranked second, with a shoot concentration of 12230 mg/kg DW when 50 mg/L Cu in solution was applied. For the four trace elements tested, the highest concentrations in shoots decreased by the order of Ni > Cu > Ph > Cd (mg/kg DW), and those in roots were Ph > Cd > Ni > Cu (mg/kg DW). Malic, oxalic and citric acids exuded by roots exposed to 1 and 50 mg/L of the metals were detected. Release of organic acids from plants significantly differed among the metal treatments. Cu was most effectively in inducing root exudation of the three types of organic acids. Cd, and Ni were also the inducers of secretion of malic and oxalic acids. With reference of Pb, a small amounts of malic and oxalic acids were detected in the root exudates, but few quantities, of citric acid were. found. However, no correlation between alternations in root exudation of organic acids and metal accumulation could be established.

关键词： heavy metal organic acid Agrogyron elongation hyperaccumulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study on Community Diversity of Coastal Shelter Forest in Jiaonan

引用

Chinese Journal of Population,Resources and Environment 2005年第3期3卷 42-47页

作者： Xu Jingwei 1,2, Li Chuanrong 3, Li Qi 4, Zheng Li 3, & Wang Weidong 1 1 Beijing forestry university,Beijing 100871,china.2 Shandong Acdemy of forestry, Jinan 250014, china.3 college of forestry, Shandong agricultural university, Taian 271018, china.4 Shandong Normal university, Jinan 250014, china Beijing forestry university Beijing 100871 China Shandong Acdemy of forestry Jinan 250014 China college of forestry Shandong Agricultural University Tatan 271018 China Shandong Normal university Jinan 250014 China

Based on the research of the plots in coastal areas in Jiaonan city, Shandong Province, the indices of richness, evenness and diversity of different community types are analyzed. The results show that: The richness indices indicates that arbor layers are close to shrub layers, and both are lower than herbage layers; the ranges among communities vary a little except those of the arbor ones. Mostly the biodiversity indices reveal that herbage, the arbor, the shrub; Biodiversity of communities has great influences on microclimate to a certain extent and is under the control of regional environment.

关键词： coastalshelterforest richness biodiversity index microclimate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Satellite RNA-mediated Reduction of Cucumber Mosaic Virus Genomic RNAs Accumulation in Nicotiana tabacum

引用

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 217-223页

作者： Qiansheng LIAO~(1,2) Liping ZHU~2 Zhiyou DU~2 Rong ZENG~(2,3) Junli FENG~2 Jishuang CHEN~(1,2*) 1 college of Life Sciences,Zhejiang university,Hangzhon 310029,china.2 Institute of Bioengineering,Zhejiang Sci-Tech university,Hangzhou 310018,china.3 college of Biological Safety Technology Hunan agricultural university,Changsha 410128,china college of Life Sciences Zhejiang UniversityHangzhou 310029 China Institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China college of Biological Safety Technology Hunan Agricultural UniversityChangsha 410128 China

Satellite RNAs(satRNAs)are molecular parasites that interfere with the pathogenesis of thehelper *** this study,the relative accumulation of cucumber mosaic virus(CMV)-Fny genomicRNAs with or without satRNAs were quantitatively analyzed by real-time *** results showed thatsatRs apparently attenuated the symptoms of CMV-Fny on Nicotiana tabacum by depressing the accumu-lation of CMV-Fny genomic RNAs,tested as open reading *** accumulation of CMV-Fny 1a,2a,2b,3a,and CP genes was much higher than that of CMV-Fny with satRs added(CMV-Fsat),at differentinoculation ***-FnyΔ2b,in which the complete 2b gene and 41 amino acids at the C-terminal of the2a gene were deleted,caused only a slight mosaic effect on *** seedlings,similar to that of CMV-Fsat,but the addition of satRs to CMV-FnyΔ2b showed further decrease in the accumulation of CMV-FnyΔ2b genomic *** results indicated that the attenuation of CMV,by adding satRs or deleting the2b gene,was due to the low accumulation of CMV genomic RNAs,and that satRNA-mediated reduction ofCMV genomic RNAs accumulation in *** was possibly related to the 2b gene.

关键词： cucumber mosaic virus satellite RNA deletion of 2b gene relative accumulation

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Research on the method for retrieving soil moisture using thermal inertia model

引用

Science china.Earth Sciences 2006年第5期49卷 539-545页

作者： LIU Zhenhua1 & ZHAO Yingshi2 1. Information college, South china.agricultural university, Guangzhou 510642, china.2. Graduate university of Chinese Academy of Sciences, Beijing 100049, china Information college South China Agricultural University Guangzhou China Graduate university of Chinese Academy of Sciences Beijing China

In order to improve accuracy of soil moisture inversion using remote sensing, a new thermal inertia model is proposed in this paper. The improved model needs only surface maximum temperature as the temperature parameter input instead of input of the surface temperature difference, as well as the surface sensible and latent fluxes are introduced into boundary conditions of thermal conductivity equation. Furthermore, surface soil conductive heat transfer equation of two-layer model is used to solve the soil thermal inertia so that the remote sensing thermal inertia method can be ap- plied to regions with better-covered vegetation, but usually only for the bare areas or worse vegetation covered areas. The model has been tested at several locations in the area of west Inner Mongolia. Comparing the simulation of the new model with the measurements obtained by apparent thermal inertia and by field test, the result shows that the inertia thermal model can be used to estimate soil moisture in more reasonable accuracy.

关键词： thermal model, thermal conductivity equation, soil initial, sensible flux, latent flux, two-layer model.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

引用

Progress in Natural Science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. college of Animal Science and Technology, china.agricultural university, Beijing 100094, china.2. college of Biological Science, china.agricultural university, Beijing 100094, china.3. college of Animal Science and Technology, Yunnan agricultural university, Kunming 650201, china. college of Animal Science and Technology China Agricultural University Beijing 100094 China college of Biological Science China Agricultural University Beijing 100094 China college of Animal Science and Technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

NEW ROBUST UNSUPERVISED SUPPORT VECTOR MACHINES

引用

Journal of Systems Science & Complexity 2011年第3期24卷 466-476页

作者： Kun ZHAO Mingyu ZHANG ~ Naiyang DENG Logistics School Beijing Wuzi University Beijing 101149 China. School of Economic and Management Beijing Jiaotong University Beijing 100044 China. college of Science China Agricultural University Bcijing 100083 China.

This paper proposes robust version to unsupervised classification algorithm based on modified robust version of primal problem of standard SVMs, which directly relaxes it with label variables to a semi-definite progra... 详细信息

关键词： Robust semi-definite programming support vector machines unsupervised learning

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ON　THE　UPPER　ESTIMATES　OF　FUNDAMENTAL　SOLUTIONS　OF　PARABOLIC　EQUATIONS　ON　RIEMANNIAN　MANIFOLDS

引用

Chinese Annals of Mathematics,Series B 1995年第1期16卷 119-130页

作者： LI JIAYU SHAO XIN (Departmellt of Mathematics, Anhui university, Hefei 230039, china.Basic Department, Anhui agricultural college, Hefei 230036, china.) ANHUI UNIV DEPT MATHHEFEI 230039PEOPLES R CHINA ANHUI AGR COLL DEPT BASICHEFEI 230036PEOPLES R CHINA

The authors first derive the gradient estimates and Harnack inequalties for positive solutions of the equation on(x, t)An(x, t) + b(x, t)' ac(￣, t) + h(x, t)u(x, t) ￣ m ￣ 0on complete Riemannian manifolds, and then derive the upper bounds of any positive L2fundamental solution of the equation when h(x, t) and b(x, t) are independent of t.

关键词： Parabolic equation Gradient estimate Harnack inequality Fundamentalsolution Riemannian manifolds.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：