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Atomic Force Microscopy-based Cell Nanostructure for Ligand-conjugated Quantum Dot Endocytosis

Acta Biochimica et Biophysica Sinica 2006年第9期38卷 646-652页

作者： Yun-Long PAN~1* Ji-Ye CAI~2 Li QIN~3.Hao WANG~4 1 The First Affiliated Hospital of jinan university,guangzhou 51063.,china 2 {3. of Life science and technology,jinan university,guangzhou 51063.,china 3.Department of Histology and Embryology,Medical {3. of jinan university,guangzhou 51063.,china 4 Information and Optoelectronic science and Engineering.South china Normal university,guangzhou 51063.,china The First Affiliated Hospital of jinan universityguangzhou 51063. China college of Life science and technology Jinan UniversityGuangzhou 510632 China Department of Histology and Embryology Medical College of Jinan UniversityGuangzhou 510632 China School for Information and Optoelectronic science and Engineering South China Normal UniversityGuangzhou 510631 China

While it has been well demonstrated that quantum dots (QDs) play an important role inbiological labeling both in vitro and in vivo,there is no report describing the cellular nanostructure basis ofreceptor-mediated ***,nanostructure evolution responses to the endocytosis of transferrin(Tf)-conjugated QDs were characterized by atomic force microscopy (AFM).AFM-based nanostructureanalysis demonstrated that the Tf-conjugated QDs were specifically and tightly bound to the cell receptorsand the nanostructure evolution is highly correlated with the cell membrane receptor-mediated ***,confocal microscopic and flow cytometry results have demonstrated the specificity anddynamic property of Tf-QD binding and *** found that the internalization of Tf-QD is linearlyrelated to ***,while the nanoparticles on the cell membrane increased,the endocytosis was stillvery active,suggesting that QD nanoparticles did not interfere sterically with the binding and function ***,ligand-conjugated QDs are potentially useful in biological labeling of cells at a nanometerscale.

关键词： quantum dot endocytosis nanostructure atomic force microscopy

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The characteristics of arc beam shaping in hybrid plasma and laser deposition manufacturing

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science china(Technological sciences) 2006年第2期49卷 238-247页

作者： ZHANG Hai’ou1, QIAN Yingping1,3. WANG Guilan1 & ZHENG Qiguang2 1. School of Material science and technology, Huazhong university of science and technology, Wuhan 43.074, china 2. Institute of Laser technology and Engineering, Huazhong university of science and technology, Wuhan 43.074, china 3. Mechanical Engineering college, Hubei university of technology, Wuhan 43.068, china School of Material science and technology Huazhong University of Science and Technology Wuhan China Mechanical Engineering college Hubei University of Technology Wuhan China Institute of Laser technology and Engineering Huazhong University of Science and Technology Wuhan China

As a new direct metal prototyping technology,the hybrid plasma and laser deposition manufacturing (PLDM) is proposed in this paper. In order to figure out the characteristics of plasma arc beam and mould in the PLDM process of high temperature alloy, the high speed CCD camera is used to obtain the picture around the plasma arc. Afterwards the sketch of picture is clearly obtained. And the effect of laser parameter, such as average power, pulse width, pulse repetition frequency and the angle between laser beam and plasma arc beam on the plasma arc appearance, is studied experimentally. The results show that the modality of plasma arc beam is markedly influenced by laser beam. And the improvements of shape precision and surface state of the layer deposited by PLDM are confirmed.

关键词： plasma hybrid laser, direct deposition, plasma arc beam, molding character, high-temperature alloy.

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Theoretical analysis of alloy multiplying factor by the empirical electron theory of solids and molecules

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science china(Technological sciences) 2007年第6期50卷 807-814页

作者： SONG YuePeng1,2, LIU GuoQuan2, LI ZhiLin3. FENG ChengMing1 1 Mechanical and Electronic Engineering college, Shandong Agricultural university, Tai’an 271018, china 2 college of Materials science and Engineering, university of science and technology, Beijing 100083. china 3.college of Material science and Engineering, Beijing university of Chemical technology, Beijing 100029, china Mechanical and Electronic Engineering college Shandong Agricultural University Tai’an China college of Materials science and Engineering University of Science and Technology Beijing China college of Material science and Engineering Beijing University of Chemical Technology Beijing China

By introducing the distribution probability of structural units in austenite contain- ing alloying elements and considering its effects on phase transformation, this paper establishes a calculation model of distribution probability of structural units. A new valence electron structure (VES) parameter-transformation effect coefficient of alloying elements (HL), is defined and then studied both theoretically and ex- perimentally. The relationship between the parameter HL and the multiplying factor (the quenching capability factor) of alloying elements is studied. The results indi- cate that the two parameters (HL and the quenching capability factor) have the same variation characteristic and substance feature. Therefore, the multiplying factor virtually expresses the relative quantity of structural units in the alloying elements-containing austenite.

关键词： multiplying factor (the quenching capability factor), empirical electron theory of solids and molecules, transformation effect coefficient of alloying elements, structural units in austenite containing alloying elements

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Microwave peak absorption frequency of liquid

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science china(Physics,Mechanics & Astronomy) 2008年第9期51卷 1254-1263页

作者： HAN GuangZe1 & CHEN MingDong2 1 Department of Physics,South china university of technology,{3. 510640,china 2 {3. Auto college,South china university of technology,{3. 510800,china Department of Physics South China University of Technology Guangzhou China guangzhou Auto college South China University of Technology Guangzhou China

Microwave-assisted extraction is a new effective method which has practical ap-plications in many fields. Microwave heating is one of its physical mechanisms,and it also has the characteristic of selectivity. When the applied microwave fre-quency equals a certain absorption frequency of the material (or specific compo-nent),the material will intensively absorb microwave energy. This is also known as resonant absorption,and the frequency is called the peak absorption frequency which depends on the physical structure of the material. In this work,dynamic hy-drogen bond energy was included in molecular activation energy; with the liquid cell model,the expression of interaction energy between dipolar molecules was derived. The rotational relaxation time was gotten from the Eyring viscosity formula. Then based on the relationship between dielectric dissipation coefficient and re-laxation time,the expression of microwave peak absorption frequency as a func-tion of the material physical structure,rotational inertia and electrical dipole mo-ment of molecules was established. These theoretical formulas were applied to water and benzene,and the calculated results agree fairly well with the experi-mental data. This work can not only deepen the study of the interaction between microwave and material,but also provide a possible guide for the experiment of microwave-assisted extraction.

关键词： microwave-assisted extraction microwave heating relaxation time liquid viscosity peak absorption fre-quency

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Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3. Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. college of Animal science and technology, {3. Agricultural university, Beijing 100094, {3. 2. college of Biological science, {3. Agricultural university, Beijing 100094, {3. 3. college of Animal science and technology, Yunnan Agricultural university, Kunming 650201, {3.) college of Animal science and technology China Agricultural University Beijing 100094 China college of Biological science China Agricultural University Beijing 100094 China college of Animal science and technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

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Scenario simulation and forecast of land use/cover in northern china

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Chinese science Bulletin 2008年第9期53卷 1401-1412页

作者： LI YueChen 1,2,3.& HE ChunYang 3.1 college of Geographical science, Chongqing Normal university, Chongqing 400047, {3. 2 college of Resources and Environmental science, Chongqing university, Chongqing 400044, {3. 3.college of Resources science & technology, Beijing Normal university, Beijing 100875, {3. college of Geographical science Chongqing Normal University Chongqing China college of Resources and Environmental science Chongqing University Chongqing China college of Resources science & technology Beijing Normal University Beijing China

Modeling land use/cover scenario changes and its potential impacts on structure and functions of ecosystem in typical regions are helpful to understanding the interactive mechanism between land use/cover system and ecosystem. Based on the analysis of the existing land use/cover simulation and forecast models, a land use/cover scenario dynamics model by the integration of System Dynamics (SD) model, Back Propagation Neural Network (BPNN) and Cellular Automata (CA) model is developed with land use/cover scenario changes in northern china in the next 3. years and simulated in this paper. The model is to simulate the land use/cover scenario demands by using a SD model at first, and then allocating the land use scenario patterns at the local scale with the considerations of land use/cover suitability, inheritance ability and neighborhood effect by using BPNN-CA model to satisfy the balance between land use/cover scenario demands and supplies. It integrates the advantages of SD, BPNN and CA. Macro-driving factors and the micro-spatial pattern are also fully taken into account. The BPNN simplifies the identification of the factors’ weights used in CA model and improves the reliability of the simulation results. The simulation accuracy of the model developed in this paper was found to be about 74%. It suggests that the model has the ability to reflect the complexity of land use/cover system at different scales to some extent and it is a useful tool for assessing the potential impacts of land use system on ecosystem. The simulated results also indicate that the urban land, water area and forest will increase significantly, and farmland and unable land will decrease gradually. Obvious land use/cover changes will take place in the farming-pastoral zone and the southeast area of northern china.

关键词：土地利用中国北方细胞自动机系统动力学

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Characteristics of fMRI BOLD signal and its neurophysiological mechanism

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Progress in Natural science:Materials International 2007年第6期17卷 630-638页

作者： Zhao Xiaohu1,2, Wu Yigen3.and Guo Shengli3.1. college of Life sciences, Zhejiang university, Hangzhou 3.0058, {3. 2. Imaging Department of Tongji Hospital of Tongji university, Shanghai 200065, {3. 3. college of Mathematics and Physics, Nanjing university of Information science and technology, Nanjing 210044, {3.) college of Life sciences Zhejiang University Hangzhou 310058 China Imaging Department Tongji Hospital Tongji University Shanghai 200065 China college of Mathematics and Physics Nanjing University of Information Science and Technology Nanjing 210044 China

The functional magnetic resonance imaging (fMRI) based on blood oxygen level dependent (BOLD) contrast has emerged as one of the most potent noninvasive tools for mapping brain function and has been widely used to explore physiological, pathological changes and mental activity in the brain. Exploring the nature and property of BOLD signal has recently attracted more attentions. Despite that great progress has been made in investigation of the characteristics and neurophysiological basis, the exact nature of BOLD signal remains unclear. In this paper we discuss the characteristics of BOLD signals, the nonlinear BOLD response to external stimuli and the relation between BOLD signals and neural electrophysiological recordings. Furthermore, we develop our new opinions regarding nonlinear BOLD response and make some perspectives on future study.

关键词： functional magnetic resonance imaging blood oxygen level dependent nonlinear response neural electrophysiological activity.

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Investigation on the Surface Coating of Grinding Balls

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Rare Metals 2001年第4期20卷 259-264页

作者： Chengguo Wang1 Yujun Bai1.2 1) college of Materials science and Engineering, Shandong university, {3. 250061, china 2) Mechanical Department. Shandong university of science and technology. {3. 25003., china 山东大学山东济南 250061

The surface coating of grinding balls was investigated experimentally. The results show that a coating may form on the surface of grinding balls when Cr or Al powders are subjected to ball grinding. The plastic deformation of the ball surface plays an important role during the coating formation, and the strong binding force between the powders and the balls is a necessary pre-condition. The thickness of coating increases with the plasticity of the powders and the balls. Annealing the balls with coating will result in an obvious diffusion of the elements in the bonding zone of interface.

关键词： coating ball grinding plastic deformation

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Boundary conditions for the bending of a piezoelectric beam

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science china(Physics,Mechanics & Astronomy) 2008年第7期51卷 847-856页

作者： GAO Yang1, XU SiPeng2 & ZHAO BaoSheng3.1 college of science, china Agricultural {3., Beijing 100083. china 2 college of Engineering, Ocean {3. of china, Qingdao 266071, china 3.School of Mechanical Engineering, {3. of science and technology Liaoning, Anshan 114044, china college of science China Agricultural University Beijing China college of Engineering Ocean University of China Qingdao China School of Mechanical Engineering University of Science and Technology Liaoning Anshan China

For beam bending in transversely isotropic piezoelectric media, the reciprocal theorem and the general solution of piezoelasticity are applied in a novel way to obtain the appropriate stress and mixed boundary conditions accurate to all orders for the beam of general edge geometry and loadings. By generalizing the method developed by Gregory and Wan, a set of necessary conditions on the edge-data for the existence of a rapidly decaying solution is established. The prescribed edge-data of the beam must satisfy these conditions in order that they could generate a decaying state within the beam. When stress and mixed conditions are imposed on the beam edge, these decaying state conditions for the case of bending deformation of piezoelectric beam are derived explicitly. They are then used for the correct formulation of boundary conditions for the beam theory solution (or the interior solution). Besides, an analytical solution of elastic beam is formulated to verify validity of our boundary conditions. For the stress data, our boundary conditions coincide with those obtained in conventional forms of beam theories. More importantly, the appropriate boundary conditions with two sets of mixed edge-data are obtained for the first time.

关键词： boundary conditions beam bending piezoelectric media decaying states regular states

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Electrochemical Characterization of Surface-modified LiMn_2O_4 Cathode Materials for Li-ion Batteries

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Journal of Wuhan university of technology(Materials science) 2006年第4期21卷 56-59页

作者：禹筱元 college of science South China Agricultural University Guangzhou 510642ChinaSchool of Physics & Engineering Sun Yat-sen University Guangzhou 510275China

To improve the performance, the surface of 12Mn2O4 was coated with very fine MgO , Al2O3.and ZnO by solgel method, respectively. The structure and morphology of the coated materials were investigated by X-ray diffraction （ XRD ）, X-ray photoelectron spectroscopy （ XPS ） and scanning electron microscopy （SEM）. The charge and discharge performance of uncoated and surfnce modified 12Mn2O4 spinel at 25℃ and 55 ℃ were tested, using a voltage window of 3.0-4.3. V and a current deasity of 0. 1 C rate. There is a slight decrease in the initial discharge capacity relative to that of uncoated UMn2 O4, bat the cycle ability of 12 12Mn2O4 coated by metal-oxide has remarkably been improved. The EIS measuremeuts of uncoated and Al2O3.-coated 12Mn2O4 were carried out by a model 273.A potentiostatl galvanistat controUed by a computer using M270 software, and using a freqnency response analyzer （ Zsimpwin ） combined with a potentiostate （ PAR 273.. Coaseqnently, the reason for the improved cycle properties is that the surface modification reduces the dissolution of Mn , which results from the suppression of the electrolyte decomposition, and suppresses the formation of passivation film that acts as an electronic insulating layer. In conclusion, the use of surface modification is an effective way to improve the electrochemical performance of 12Mn2O4 cathode material for lithium batteries.

关键词： lithium-ion batteries surface modification metal-oxide coating: 12Mn2O4 cathode

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