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Journal of Iron and Steel research International 2011年第S1期18卷 696-700页

作者： LIU Sheng-xin 1,2,LIU Guo-quan 2,CHEN Yong 1,ZHANG Yong-gang 3,HUANG Jian-kai 3 (1.School of Materials science and Engineering,Zhengzhou University,Zhengzhou 450002,Hennan china 2.School of Materials science and Engineering,University of science and technology Beijing,100083,china 3.wuxi Seamless Steel Tube Company Ltd,wuxi 214026,Jiangsu china) Zhengzhou Univ Sch Mat Sci & Engn Zhengzhou 450002 Hennan Peoples R China Univ Sci & Technol Beijing Sch Mat Sci & Engn Beijing 100083 Peoples R China wuxi Seamless Steel Tube Co Ltd Wuxi 214026 Jiangsu Peoples R China

The relationship between microstructures and mechanical properties of a medium carbon V-N microalloyed steel used for N80 seamless oil-well tubes of hot rolling non-quenched/tempered (non-Q and T) was *** results have shown that volume percentages of upper bainite,modified bainite and ferrite have a decisive influence on impact energies of steel *** the total volume percentage of bainite is larger than 5%,the impact energy of tubes can not satisfy with the industrial ***,if the total volume percentage of bainite is smaller than 5%,then the impact energy of steel tubes enhances with volume percentage of ferrite *** final microstructures have closely relation with tube billet quality,controlled cooling temperature after tube rolling and cooling method after *** quality of medium carbon V-N microalloyed steel for non-Q and T oil-well tubes can be produced through comprehensive control of microstructures and mechanical properties in sub-procedures,especially for tube billet quality and controlled cooling parameters.

关键词： medium carbon V-N microalloyed steel N80 grade seamless oil-well tube microstructure mechanical property

Subchronic toxicological effects of aquatic nitrobenzene on Medaka and Chinese rare minnow

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science china Chemistry 2007年第5期50卷 707-717页

作者： ZHOU QunFang1, FU JianJie1,2, MENG HaiZhen1,2, ZHU XueYan1,2, JIANG GuiBin1, ZHANG JianBin1, LIU JieMin2 & SHI GuoQing2 1 State Key Laboratory of environmental Chemistry and Ecotoxicology, research center for Eco-environmental sciences, Chinese Academy of sciences, Beijing 100085, china 2 Department of Chemistry, School of Applied science, University of science and technology Beijing, Beijing 100083, china State Key Laboratory of environmental Chemistry and Ecotoxicology ResearchCenter for Eco-environmental SciencesChinese Academy of SciencesBeijing 100085China Department ofChemistry School of Applied ScienceUniversity of Science and Technology B

Based on the simulative exposure experiments of nitrobenzene to two small experimental fishes, Medaka (Oryzias latipes) and Chinese Rare Minnow (Gobiocypris rarus), it was found that nitrobenzene could decrease in a linear way in the static aquatic system. Both fishes could accumulate dose-related levels of nitrobenzene quickly and eliminate the compound rapidly when they were transferred to clean water. The Chinese rare minnow showed more sensitivity to the acute toxicity exposure of nitrobenzene than Medaka. Typical molecular biomarkers in oxidative defense system including superoxide dismu- tase (SOD), catalase (CAT) showed the chemical induced alterations. The changes of acetylcholi- nesterase (AChE) activities indicated that nitrobenzene might affect the normal neural function. Nitro- benzene exposure could also lead to obvious damaging effects on the target organs, such as gill and liver by hitopathological studies.

关键词： nitrobenzene, Medaka, Chinese rare minnow, bioaccumulation, elimination, molecular biomarkers, histopathological effects

Ultrasound/Microbubble Enhances Foreign Gene Expression in ECV304 Cells and Murine Myocardium

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 824-831页

作者： Dongping GUO, Xiaoyu LI, Ping SUN, Zhiguang WANG, Xiuying CHEN, Qi CHEN, Leming FAN*, Bin ZHANG1, Lizheng SHAO1, and Xiaorong LI1 Atherosclerosis research center, Nanjing Medical University, Nanjing 210029, china 1 Cardiovascular Division, wuxi No. 1 Hospital, wuxi 214002, china Atherosclerosis research center Nanjing Medical UniversityNanjing 210029 China Cardiovascular Divisionwuxi No. 1 Hospital Wuxi 214002 China

Although viral vectors are efficient systems to transfer foreign genes into cells or target tissues, safety issues remain in relation to human gene therapy. Microbubbles currently used as ultrasound contrast agents have been applied in transfection of genes. This study was designed to test the transfection efficiency and the expression of exogenous gene mediated by ultrasound irradiation enhanced air filled albumin microbubbles in ECV304 cell line in vitro and the heart of the mouse in vivo. Air filled microbubbles (2.0– 4.0 μm in diameter) were created by sonicating the mixture of human albumin, glucose, mannitol and special additive that was designed for stabilization. Plasmid DNA loading the reporter geneswas gently mixed with microbubbles. The mixture of plasmid DNA and microbubbles was administrated to cultured ECV304 cells and BALB/c mice (tail vein injection) under different ultrasound/microbubble conditions, and then the trans- fection and expression efficiency were examined. The results both in vivo and in vitro demonstrated that microbubble with ultrasound irradiation could significantly elevate the exogenous gene expression as compared with microbubble or ultrasound only. Overall, the present study showed that the ultrasound-target microbubble destruction method enhanced the exogenous gene expression in vivo and in vitro, and provided a gene therapy way not only efficient but also easy to be manipulated and carried out in clinical.

关键词： microbubble ultrasound transfection gene therapy

Dynamics of picoplankton in the Nansha Islands area of the South china Sea

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Acta Oceanologica Sinica 2004年第3期23卷 493-504页

作者： YANGYanhui JIAONianzhi KeyLaboratoryforMarineenvironmentalscienceoftheMinistryofEducation EnvironmentalScienceResearchCenterXiamenUniversityXiamen361005China

Dynamics of major picoplankton groups, Synechococcus (Syn), Prochlorococcus (Pro), picoeukaryotes (Euk) and heterotrophic bacteria (Bact) was investigated by flow cytometry for the first time in the Nansha Islands area in the South china Sea. Averaged over the whole investigation area, depth-weighted integrated cell abundance (DWA) of Syn, Pro, Euk and Bact was 1.6 (0.4-5.7)×103, 5.4 (0.1-7.3)×104, 0.7 (0.2-2.2)×103, and 2.3 (1.4-3.2)×105 cells/mL respectively. Picoautotrophic cell abundance was low in the northwest part of the Nansha Islands where surface water temperature was low and the upper mixed layer was shallow. Concurrently, a surface maximum vertical distribution pattern was observed in this area. While in the southeast and east zones where temperatures were relatively higher and nitraclines were deeper, picoplankton is abundant and a subsurface maximum around 50-75 m is observed. Coupling of horizontal and vertical distribution patterns of picoplankton abundance and hydrological status was found, suggesting a strong influence of currents and water column structure on picoplankton distribution in the investigation area. Contrary to that in the shelf water in the East china Sea, the relationship between Pro and Bact in the Nansha Islands area in the South china Sea was not significantly negative but weakly positive. Moreover, a similar distribution pattern of Syn and Pro was observed. Possible reasons for these differences in the two marine regimes were discussed.

关键词： Synechococcus Prochlorococcus pico-eukaryotes heterotrophic bacteria Nansha Islands area South china Sea

Temperature-controlled ionic liquid dispersive liquid phase microextraction combined with ultra-high-pressure liquid chromatography for the rapid determination of triclosan,triclocarban and methyl-triclosan in aqueous samples

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science china Chemistry 2010年第12期53卷 2600-2607页

作者： GUO JieHong 1,2,LI XingHong 2,CAO XueLi 1,QU Lei 2,3,HOU DeKun 2 & XU XiaoBai 2 1 School of Chemical and environmental Engineering,Beijing technology and Business University,Beijing 100048,china 2 State Key Laboratory of environmental Chemistry and Eco-toxicology research center of Eco-environmental sciences,Chinese Academy of sciences,Beijing 100085,china 3 Zhengzhou University,Zhengzhou 450001,china 1. School of Chemical and environmental Engineering Beijing Technology and Business University Beijing 100048 China2. State Key Laboratory of Environmental Chemistry and Eco-toxicology research center of Eco-environmental sciences Chinese Academy of Sciences Beijing 100085 China3. Zhengzhou University Zhengzhou 450001 China

As extraction solvents,ionic liquids have green *** this study,an environmentally benign analytical method termed temperature-controlled ionic liquid dispersive liquid phase microextraction (TIL-DLME) combined with ultra-highpressure liquid chromatography (UHPLC)-tunable ultraviolet detection (TUV) was developed for the pre-concentration and determination of triclosan (TCS),triclocarban (TCC) and methyl-triclosan (M-TCS) in water *** parameters that may affect extraction efficiencies were examined and optimized,including the types and amount of ionic liquids,volume of the diluent,heating temperature,cooling time,salt effect and pH *** the optimum conditions,linearity of the method was observed in the ranges of 0.0100-100 μgL-1 for TCS and M-TCS,and 0.00500-50.0 μgL-1 for TCC with correlation coefficients (r2) > *** limits of detection (LODs) ranged from 1.15 to 5.33 *** in domestic water and TCC in reclaimed water were detected at the concentrations of 1.01 and 0.126 μgL-1,*** spiked recoveries of the three target compounds in reclaimed water,irrigating water,waste water and domestic water samples were obtained in the ranges of 68.4%-71.9%,61.6%-87.8%,58.9%-74.9% and 64.9%-92.4%,*** with the previous dispersive liquid-liquid microextraction method (DLLME) about the determination of TCS,TCC and M-TCS,this method is not only more environmentally friendly but also more sensitive.

关键词： ionic liquid temperature-controlled ionic liquid dispersive liquid phase microextraction UHPLC aqueous samples

Reduced-glutathione concentrations in Boleophthalmus pectinirostris tissues exposed to benzo(a)pyrene

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Journal of environmental sciences 2001年第3期13卷 308-310页

作者： FENG Tao 1, ZHENG Wei yun 1, GUO Xiang\|qun\+2, ZHANG Yong\+1(1.environmental science research center, Xiamen University, Xiamen 361005, china 2.Chemistry Department, Xiamen University, Xiamen\ 361005, china) environmental science research center Xiamen University Xiamen 361005 China.

The concentrations of reduced-glutathione ( GSH) in liver and ovary of Boleophthalmus pectinirostris are quantified. The concentrations of GSH in the ovary are much higher than that of GSH in the liver(nearly 3 times of the liver). The study also investigates the changes of GSH contents in the two organs while the fishes were exposed to benzo(a)pyrene(BaP) at concentrations of 0, 0.05, 0.2 and 0.5 mg/L respectively for up to a week. The concentrations of GSH in the liver of BaP-exposed fish increased significantly with dose, whereas the concentrations of GSH in the ovary decreased significantly compared to controls. The results suggested that both the liver and the ovary are the primary organ in BaP metabolism, and that the changes of GSH levels may represent an adaptive response or toxic effect to Bap exposure.

关键词： Boleophthalmus pectinirostris benzo(a)pyrene reduced-glutathione

Performance and mechanism study for low-temperature SCR of NO with propylene in excess oxygen over Pt/TiO_2 catalyst

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Journal of environmental sciences 2010年第9期22卷 1441-1446页

作者： Zhixiang Zhang,Mingxia Chen,Zhi Jiang,Wenfeng Shangguan research center for Combustion and environmental technology,Shanghai Jiao Tong University,Shanghai 200240,china. [a] research center for Combustion environmental technology Shanghai Jiao Tong University Shanghai 200240 China

A 0.5 wt.% Pt/TiO2 catalyst was prepared and used for the low-temperature selective catalytic reduction （SCR） of NO with C3H6 in the presence of excess oxygen. The effects of Pt loading and 02 concentration on Pt/TiO2 catalytic performance for low-temperature SCR were investigated. It was found that optimal Pt loading was 0.5 wt.% and excess 02 favored low-temperature SCR of NOx. The mechanism of low-temperature SCR of NO with C3H6 was investigated with respect to the behavior of adsorbed species over Pt/TiO2 at 150~C using in situ DRIFTS. The results indicated that surface nitrosyl species （Pt~＋-NO and Ti3＋-NO） and pt2＋-CO are main reaction intermediates during the interactions of NO, C3H6 and 02. A simplified NO decomposition mechanism for the low-temperature SCR of NO with C3H6 was proposed.

关键词： NO low-temperature SCR Pt/TiO2 in situ DRIFTS

Preparation and characterization of nitrogen-doped titanium dioxides

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science china Chemistry 2007年第2期50卷 212-216页

作者： ZHU GuoPing1 & ZHOU KangGen2 1 environmental science research & Design Institute of Zhejiang Province,Hangzhou 310007,china 2 Department of environmental Engineering,School of Metallurgical science and Engineering,Central South University,Changsha 410083,china 1. environmental science research & Design Institute of Zhejiang Province Hangzhou 310007 China 2. Department of environmental Engineering School of Metallurgical Science and Engineering Central South University Changsha 410083 China

A type of high visible-light active titanium oxinitride(TiO2-xNx) powder was prepared by a simple proc-ess:the calcination of the hydrated titanium dioxide at the atmosphere of ammonia-argon using a tu-bular electric furnace at high temperatures. The hydrated titanium dioxide was synthesized as the precursor of TiO2-xNx using titanic acid as raw material,which came from sulfate technique of produc-ing titanium white. The effects of temperature and reaction time on the nitrogen content,grain size and crystal structure were studied. The visible-light activity and photocatalysis capability of the powder were also investigated.

关键词： nitrogen-doped titanium dioxides visible-light activity photocatalysis titanium oxinitride

Ecological hydrograph based on Schizothorax chongi habitat conservation in the dewatered river channel between Jinping cascaded dams

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science china(Technological sciences) 2011年第S1期54卷 54-63页

作者： LI RuoNan 1,CHEN QiuWen 1,2 & Duan Chen 1,3 1 research center for Eco-environmental science,Chinese Academy of sciences,Beijing 100085,china 2 china Three Gorges University,Yichang 443002,china 3 Hydraulics Department,Changjiang River Scientific research Institute,Wuhan 430010,china 1. research center for Eco-environmental science Chinese Academy of Sciences Beijing 100085 China 2. china Three Gorges University Yichang 443002 China 3. Hydraulics Department Changjiang River Scientific Research Institute Wuhan 430010 China

Hydropower development changes the river hydrological regime,thereby altering river ecosystem *** important measure for reducing degradation of ecosystem is to determine ecological flow and implement it by reservoir *** paper described a method to calculate river ecological flow based on fish habitat conservation and applied it to Jinping River Bend of Yalong *** chongi was selected as target species,and the fish habitat model coupling with water environmental model was developed according to the relationship between fish and water environment ***,improved hydraulic habitat suitability index(IHHS) and habitat fragment index(HFI) varying with discharge were discussed in this *** area representing average annual flow was taken as a reference,and then ecological hydrographs under different habitat conservation levels were calculated considering season variation and life stages of *** to the natural condition,the model results indicated that the reservoir operation clearly influenced the habitat of the target *** was proposed to ensure flow releases to maintain 70%-90% habitat in section after joint while about 60% before joint.

关键词： river habitat habitat assessment ecological flow ecological hydrograph

Phase behavior of TXs/toluene/water microemulsion systems for solubilization absorption of toluene

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Journal of environmental sciences 2010年第2期22卷 271-276页

作者： Lian Liu,Senlin Tian ,Ping Ning Faculty of environmental science and Engineering,Kunming University of science and technology,Kunming 650093,china [a] Faculty of environmental science Engineering Kunming University of Science technology Kunming 650093 China

Triton Xs （TXs） surfactants/cosurfactant/water/oil （toluene） microemulsion systems for enhancing toluene solubilization were proposed and its potential was investigated for toluene removal from gas stream. The results indicated that TX-100 was superior to other TXs surfactants in removing toluene without cosurfactant. The efficiency of cosurfactants for improving toluene solubilization capacity follows the order： amine 〉 alcohol 〉 acid. According to the factor analysis, the linear cosurfactants are better than the branched ones. The effects of hydrophile-lipophile balance （HLB）, salt （NaCl） concentration and temperature on the formation of microemulsion system were also discussed. The results suggested that the optimum value of HLB was 15, the effect of NaCl concentration on the system was inconspicuous and the lower temperature enhanced the solubilization capacity. Nonionic surfactant-based microemulsions had a significant absorption enhancement for toluene, indicated by as much as 82.72% of toluene in phase composition diagram, which will have a great prospect in air pollution treatment.

关键词： microemulsion phase behavior absorption toluene