检索结果-南通市图书馆

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

science china Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2, ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of environmental Chemistry and Ecotoxicology, research center for Eco-environmental sciences, Chinese Academy of sciences, Beijing 100085, china 2Department of Chemistry, Tsinghua University, Beijing 100084, china 3Beijing Chemclin Biotech Co., Ltd., Beijing Academy of science and technology, Beijing 100094, china 4School of Chemistry and Chemical Engineering, Xianyang Normal College, Xianyang 712000, china 1. State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hydrogen metabolic pathways of Rhodospirillum rubrum under artificial illumination

引用

Chinese science Bulletin 2010年第1期55卷 32-37页

作者： ZHU RuiYan1,2,3 & LI JiLun1 1 College of Biological sciences, State Key Laboratory for Agrobiotechnology, china Agricultural University, Beijing 100193, china 2 College of environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, china 3 Engineering research center for Bioenergy of Hebei Province, Qinhuangdao 066004, china College of Biological sciences State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China College of environmental and Chemical Engineering Yanshan University Qinhuangdao 066004 China Engineering research center for Bioenergy of Hebei Province Qinhuangdao 066004 China

Multiple enzymes involved in hydrogen metabolism have been detected in photosynthetic bacterium Rhodospirillum rubrum un-der various growth conditions. To investigate hydrogen metabolic pathways and the contribution of each pathway to hydrogen photoproduction in R. rubrum under the artificial illumination condition, three mutants were constructed, including nifHanfDG double mutant lacking both Fe-nitrogenase and Mo-nitrogenase, anfDG mutant lacking Fe-nitrogenase and nifHhupL double mu-tant (uptake hydrogenase deficient mutant). Comparison of the hydrogen production of these 3 mutants with R. rubrum wild type and the uptake hydrogenase deficient mutant showed that there was a third pathway involved in hydrogen production besides Mo-nitrogenase and Fe-nitrogenase, which mainly contributed to hydrogen evolution. Only a small portion of hydrogen was gen-erated by the third pathway. The hydrogen produced by Mo-nitrogenase, Fe-nitrogenase and the third pathway was about 93.5%, 4.9% and 1.5%, respectively, while the hydrogen consumed by uptake hydrogenase was about 13.3%. The investigation of the formate-linked hydrogenase activity indicated that the third pathway for hydrogen production was not mediated by the for-mate-linked hydrogenase, but probably by some unknown enzyme.

关键词：氢代谢人工照明代谢途径红螺菌固氮酶脱氢酶突变体体吸收

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bacterial community in deep subseafloor sediments from the western Pacific "warm pool"

引用

science china Earth sciences 2008年第4期51卷 618-624页

作者： ZHAO Jing1 & ZENG RunYing2 1 College of Oceanography and environmental science, Xiamen University, Xiamen 361005, china 2 Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, china College of Oceanography and environmental science Xiamen University Xiamen 361005 China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

The bacterial community in deep subseafloor sediments at a depth of 230 cm from the western Pacific "warm pool" is studied by construction of 16S rDNA clone library and PCR-RFLP (Restriction Fragment Length Polymorphism) analysis. The results indicate that the bacterial community in these sediments is mainly composed of five groups: α-Proteobacteria, β-Proteobacteria, CFB group (Cytophaga / Flexi-bacteria / Bacteroides), Acidobacteria and gram positive bacteria, with a few genera detected in each group. The most abundant bacteria group is α-Proteobacteria, and the next is β-Proteobacteria. The dominant species in α-and β-Proteobacteria are Sphingomonas paucimobilis and Pseudomonas alca-ligenes respectively. The CFB group is simply composed of members belonging to Flavobacterium. The gram positive bacteria are rich, and mainly consists of the genus Geobacillus. The analysis of bacterial community indicates that organic matter is still abundant in the subseafloor sediments at the depth of 230 cm in the western Pacific "warm pool" . These bacteria in this deep biosphere may play an impor-tant role in the nitrogen cycle of deep sea sediments at "warm pool" .

关键词： western Pacific 'warm pool' deep subseafloor sediment bacterial community

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Assimilation of temperature and salinity using isotropic and anisotropic recursive filters in Tropic Pacific

引用

Acta Oceanologica Sinica 2011年第1期30卷 15-23页

作者： LIU Ye ZHAO Yanling National Marine environmental Forecasting center Beijing 100081 China No 61741 Army Troop Beijing 10081 China

A data assimilation scheme used in the updated Ocean three-dimensional Variational Assimila- tion System （OVALS）, OVALS2, is described. Based on a recursive filter （RF） to estimate the background error covariance （BEC） over a predetermined scale, this new analysis system can be implemented with anisotropic and isotropic BECs. Similarities and differences of these two BEC schemes are briefly discussed and their impacts on the model simulation are also investigated. An idealized experiment demonstrates the ability of the updated analysis system to construct different BECs. Furthermore, a set of three years experiments is implemented by assimilating expendable bathythermograph （XBT） and ARGO data into a Tropical Pacific circulation model. The TAO and WOA01 data are used to validate the assimilation results. The results show that the model simu- lations are substantially improved by OVALS2. The inter-comparison of isotropic and anisotropic BEC shows that the corresponding temperature and salinity produced by the anisotropic BEC are almost as good as those obtained by the isotropic one. Moreover, the result of anisotropic RF is slightly closer to WOA01 and TAO than that of isotropic RF in some special area （e.g. the cold tongue area in the Tropic Pacific）.

关键词： recursive filter anisotropic isotropic background error covariance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

甲壳素纯度的高效液相色谱法测定

引用

分析测试学报 2003年第4期22卷 9-13页

作者：朱晓兰夏文水中国科技大学烟草研究中心安徽合肥230052 无锡轻工业大学食品学院江苏无锡214036

由于生物材料中甲壳素或壳聚糖是大分子 ,不溶于水 ,以及与蛋白质等结合在一起而难以定量测定 ,工业生产中尚无甲壳素或壳聚糖纯度的标准测定方法 ;采用甲壳素水解生成氨基葡萄糖 ,再用氯甲酸芴甲酯(FMOC_Cl)进行衍生 ,利用液相色谱技... 详细信息

由于生物材料中甲壳素或壳聚糖是大分子 ,不溶于水 ,以及与蛋白质等结合在一起而难以定量测定 ,工业生产中尚无甲壳素或壳聚糖纯度的标准测定方法 ;采用甲壳素水解生成氨基葡萄糖 ,再用氯甲酸芴甲酯(FMOC_Cl)进行衍生 ,利用液相色谱技术对衍生产物进行分析 ;结果表明 ,HPLC法是生物材料中甲壳素的一种灵敏的、特有的定性定量分析方法。

关键词：甲壳素纯度高效液相色谱法测定衍生化氨基葡萄糖盐酸盐

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Simulation study on vertical transport of atrazine in soil column

引用

Journal of environmental sciences 2000年第3期12卷 303-309页

作者： YE Chang ming, GONG Ai jun, LEI Zhi fang, WANG Xing jun, ZHENG He hui (research center for Eco-environmental sciences, Chinese Academy of science, Beijing 100085, china. E\|mail: yecm@***) mail.rcees. ac. cn

A simulation experiment on vertical transport of herbicide atrazine (2 chloro 4 ethylamino 6 isopropylamino s triazine) in soil column was conducted using lysimeter system. The atrazine concentrations in leaching water and soil samples in column at 8 layers with 10 cm thick of each layer were detected by high performance liquid chromatography. The results show total atrazine amount in leaching water increases nonlinearly with the leaching time and the herbicide application rate, and the atrazine concentrations in column soil decrease with the vertical depth after water leaching. The distribution of atrazine mass in the system after 154 days were that among the applied atrazine, 0.3% is out through leaching water, 1% is methanol extractable residues in soil, 46% is methanol nonextractable residues in soil, and 52% is other loss (including volatilization and degradation). The study indicates movement of atrazine in agriculture soil may not only have relation to the properties of herbicide, but also to the herbicide application history.

关键词： soil lysimeter atrazine vertical transport sorption distribution

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

引用

Acta Oceanologica Sinica 2009年第2期28卷 103-114页

作者： HOU Jianjun LAI Hongyan HUANG Bangqin CHEN Jixin College of Fisheries Huazhong Agricultural University Wuhan 430070 China College of Life sciences Hubei Normal University Huangshi 435002 China State Key Laboratory of Marine environmental science Environmental Science Research Center Xiamen University Xiamen 361005 China Key Laboratory of Freshwater Fish Germplasm Resources and Biotechnology Yangtse River Fisheries Research Institute Jinzhou 434000 China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes （DNA probes） were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with fluorescein isothiocyanate entered the algal cells and bound to target sequences, and the fluorescence signal resulting from whole-cell hybridization varied from probe to probe. These DNA probes and the hybridization protocol we developed were specific and effective for P. minimum and T. pulchella, without any specific binding to other algal species. The hybridization efficiency of different probes specific to P. minimum was in the order： PM18S02 PM28S02 〉 PM28S01 〉PM18S01, and that of the probes specific to T. pulcheUa was TP18S02 TP28S01 〉 TP28S02 〉TP18S01. The different hybridization efficiency of the DNA probes could also be shown in the fluorescent signals between the labeled and unlabeled cells demonstrated using flow cytometry. The DNA probes PM18S02, PM28S02, TP18S02 and TP28S01, and the protocol, were also useful for the detection of Mgae in natural samples.

关键词： oligonucleotide DNA probes Prorocentrum minimum Takayama pulcheUa fluorescence in situ hybridization flow cytometry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Degradation of furan tebufenozide in laboratory and field trials

引用

science china Chemistry 2010年第8期53卷 1818-1824页

作者： GUO Cong,LI DaHui,CHEN JinHui,GUO BaoYuan,WANG HuiLi & LI JianZhong research center for Eco-environmental sciences,Chinese Academy of sciences,Beijing 100085,china 1. research center for Eco-environmental sciences Chinese Academy of Sciences Beijing 100085 China

Furan tebufenozide is a newly developed insect growth regulator and has been applied as a pesticide in agriculture in *** degradation under both laboratory and field conditions was investigated,and the degradation kinetics was fitted by simple first order kinetics (SFO) model and first order double exponential (FOD) *** studies were conducted with or without light in five simulated media (sterilized deionized water,river water,soil solution,sterilized soil and natural soil).No dissipations of furan tebufenozide were observed in sterilized aqueous and soil media under light prevented conditions,whereas degradation occurred under all the other conditions in the *** from SFO and FOD models,DT50 in the dark and light laboratory conditions was in the range of 39.7-82.5 and 1.1-8.0 days,*** results indicated that microbes and light were the main factors for the degradation of the pesticide in the *** field trials,derived from the SFO model,DT50 and DT90 were 30.3 and 100.5 days,while derived from the FOD model,DT50 and DT90 were 28.9 and 274.9 days,*** with laboratory experiments,field trials were influenced by multiple ***,the SFO model could not fit experimental data as well as the FOD model did in field trials.

关键词： uran tebufenozide dissipation soil water degradation kinetics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Application analysis of empirical mode decomposition and phase space reconstruction in dam time-varying characteristic

引用

science china(Technological sciences) 2010年第6期53卷 1711-1716页

作者： ZHANG ZhiJun1,2,3,GU ChongShi1,2,BAO TengFei1,2,ZHANG Lan1,2 & YU Hong1,2 1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University,Nanjing 210098,china 2 National Engineering research center of Water Resources Efficient Utilization and Engineering Safety,Hohai University,Nanjing 210098,china 3 Department of Civil,environmental and Geomatic Engineering,Swiss Federal Institute of technology,Zurich 8093,Switzerland 1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 China2. National Engineering Research Center of Water Resources Efficient Utilization and Engineering Safety Hohai University Nanjing 210098 China3. Department of Civil Environmental and Geomatic Engineering Swiss Federal Institute of Technology Zurich 8093 Switzerland

In view of some courses of the time-varying characteristics processing in the analysis of dam deformation,the paper proposes a new method to analyze the dam time-varying characteristic based on the empirical mode decomposition and phase space reconstruction *** of all,to reduce the influences on the traditional statistical model from human factors and assure the analysis accuracy,response variables of the time-varying characteristic are obtained by the way of the empirical mode decomposition;and then,a phase plane of those variables is reconstructed to investigate their processing *** methods have already been applied to an actual project and the results showed that data interpretation with the assists of empirical mode decomposition and phase space reconstruction is effective in analyzing the perturbations of response variables,explicit in reflecting the entire development process,and valid for obtaining the evolution rules of the time-varying *** methodology is a powerful technical support for people to further master the rules of dam operation.

关键词： dam safety monitoring dam time-varying characteristic empirical mode decomposition phase space reconstruction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preparation and characterization of a novel adsorbent for removing lipophilic organic from water

引用

science china Chemistry 2005年第6期48卷 600-604页

作者： LIU Huijuan1, DAI Ruihua1, QU Jiuhui1 & RU Jia1,2 1. State Key Laboratory of environmental Aquatic Chemistry, research center for Eco-environmental sciences, Chinese Academy of sciences, Beijing 100085, china 2. Graduate School of the Chinese Academy of sciences, Beijing 100039, china 1. State Key Laboratory of environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences 100085 Beijing China 2. Graduate School of the Chinese Academy of sciences 100039 Beijing China

A novel composite adsorbent containing a kind of lipid-triolein is studied. The ad-sorbent is prepared by embedding triolein into cellulose acetate (CA) sphere. The preparation method, the physical-chemical properties of the adsorbent and the removal efficiency of two or-ganochlorinated pesticides are studied. The adsorbent is stable in water and no triolein leaks into water for 465 h soaking. The adsorbent has high adsorption capacity for organochlorinated pes-ticides such as dieldrin and aldrin. The results suggest that triolein-containing adsorbent could serve as a good adsorbent for lipophilic organic pollutants. The adsorption rate for lipophilic pol-lutants is very fast and has relation with the logKow of the compounds.

关键词： triolein, cellulose acetate, adsorbent, lipophilic organic, drinking water.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：