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Acta Biochimica et Biophysica Sinica 2007年第6期39卷 391-398页

作者： Xiaoyu LIU~(1#), Wen LIU~(1#), Ling YANG~(1#), Beili XIA~1, Jinyan LI~1, Ji ZUO~(1*), and Xiaotian LI~(2*) 1 Department of Celhdar and Genetic Medicine, shanghai medical College. fudan university, shanghai 200032. china 2 the obstetrics and gynecology hospital of the medical center of fudan university, shanghai 200032. china Department of Cellular and Genetic Medicine Shanghai Medical College Fudan UniversityShanghai 200032 China The obstetrics and gynecology hospital of the medical center of fudan universityshanghai 200032 China

The change of connexin 43 （Cx43） expression and the biological behaviors of Cx43 in rat heart cell line H9c2, expressing Wnt-3a （wingless-type MMTV integration site family, member 3A） were evaluated in the present study. Plasmid pcDNA3.1/Wnt-3a was constructed and transferred into H9c2 cells. The cell model Wnt-3a+-H9c2 steadily expressing Wnt-3a was obtained. Compared with H9c2 and pcDNA3.1-H9c2 cells, the expression of Cx43 in Wnt-3a+-H9c2 cells was clearly increased, the proliferation of Wnt-3a+-H9c2 cells was significantly changed, and cell migration abilities were also improved （P2 and pcDNA3.1-H9c2 cells, the G2phase of the cell cycle increased by 11% in Wnt-3a+-H9c2 cells. Thus, Wnt-3a overexpression is associated with an increase in Cx43 expression and altered migratory and proliferative activity in H9c2 cells. Cx43 might be one of the downstream target genes regulated by Wnt-3a.

关键词： Wnt-3a Cx43 H9c2 cell line cell migration

Phylogeographic analysis of human papillomavirus 58

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Science china(Life Sciences) 2009年第12期52卷 1164-1172页

作者： LI YanYun1, LI ZuoFeng2, HE YiFeng1, KANG Yu1, ZHANG XiaoYan1, CHENG MingJun1, ZHONG Yang2, 3· & XU CongJian1· 1 obstetrics and gynecology hospital of fudan university, shanghai 200011, china 2 shanghai Bioinformation Research center, shanghai 200235, china 3 School of Life Sciences, fudan university, shanghai 200433, china obstetrics and gynecology hospital of fudan university Shanghai China shanghai Bio-information Research center Shanghai China School of Life Sciences Fudan University Shanghai China

Human papillomavirus 58 (HPV58) is one type of HPV with high risk of causing cervical cancer. Unusually high prevalence of HPV58 has been reported in Asia, Africa and some other areas. However, due to the scattered distribution of global data, in addition to the lack of data of some HPV58 high-incidence nations and regions, like china's Mainland, a comprehensive analysis of the global geographical distribution of HPV58 remains blank so far. In this study, HPV58 from the human cervical cancer tissue was detected in china's Mainland, and 14 new HPV58-E6/L1 gene sequences were obtained. Moreover, phylogeographic analysis has been conducted combining the HPV58 sequences that have been deposited in GenBank since 1985. The study result shows that the sequences detected from the shanghai, Jiangsu and Sichuan areas are homologous with those found in the past from Hong Kong and Xi'an, china, as well as Japan and other Southeast Asian areas. Furthermore, Western Africa is considered to be the "root" source of the HPV58 variant, while china's Mainland and Southeast Asia are "transit points" and the new sources of HPV58 after receiving the isolates from the "root" source; like HPV16 and HPV18, the HPV58 might also be one of the major HPV types associated with the development and spread of cervical cancer.

关键词： human papillomavirus 58 (HPV58) cervical cancer phylogeography molecular evolution

Is There A Relationship between Polycystic Ovary Syndrome and Endometriosis?

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Journal of Reproduction and Contraception 2011年第3期22卷 177-182页

作者： Xue-ya QIAN Hao WU Xiao-wei XI Department of obstetrics and gynecology Guangzhou Women and Children＇s Medical Center Guangzhou 510623 China Department of obstetrics and gynecology Shanghai First people＇s Hospital Affiliated to Shanghai JiaoTong University Shanghai 200011 China

The endocrinologic and metabolic abnormalities of women with polycystic ovary syndrome （PCOS） can result in a series of endometrial diseases. Abnormalities of hyperandrogenism and hyperinsulinemia that may be found in PCOS can elevate the levels of E2 indirectly, reduce progesterone secretion and induce some growth factors such as vascular endothelial growth factor （VEGF） and insulin-like growth factors （IGFs） over expression, which may have a major impact on endometriosis occurrence and development. We suppose that there is a possible connection between PCOS and endometriosis.

关键词： polycystic ovary syndrome (PCOS) endometriosis (EMs) estrogen metabolism growth factors

PROMISING EFFECTIVENESS ON WOMEN’S REPRODUCTIVE HEALTH IN INTEGRATIVE MEDICINE

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PROMISING EFFECTIVENESS ON WOMEN’S REPRODUCTIVE HEALTH IN I...

更年期大鼠血清雌二醇水平与脾细胞凋亡的增龄性改变

The 5th International Conference on Reproductive Endocrinology

作者： Yu Jin obstetrics and gynecology hospital fudan university shanghai china

＜正＞Objectives:The Integrative Medicine（integration of traditional Chinese medicine and western medicine）with holistic and dialectic viewpoints picks up one’s strong parts to offset other’s weakness, which leads to perfect results and long-term benefits to patients and human beings. Methods:Ovulation can be induced by acupuncture via depletion of central β-endorphin levels resulting in LH/FSH surge,thus the first candidate is proved to be the pubertal menstrual disorder.

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复旦学报（医学版） 2004年第2期31卷 155-157页

作者：哈灵侠张绍芬谢倩刘暘复旦大学附属妇产科医院妇科上海200011 中山医院实验研究中心上海200032

目的　了解更年期大鼠雌激素水平与脾细胞凋亡的增龄性变化。方法　应用放射免疫法测定血清雌二醇 (E2 )水平 ;AnnexinV FITC/PI双染色流式细胞仪测定脾细胞凋亡率 ;逆转录聚合酶链反应 (RT PCR)检测脾细胞凋亡基因Bcl 2、Bax的表达。... 详细信息

目的　了解更年期大鼠雌激素水平与脾细胞凋亡的增龄性变化。方法　应用放射免疫法测定血清雌二醇 (E2 )水平 ;AnnexinV FITC/PI双染色流式细胞仪测定脾细胞凋亡率 ;逆转录聚合酶链反应 (RT PCR)检测脾细胞凋亡基因Bcl 2、Bax的表达。结果　更年组大鼠血E2 水平低于青年组 (14 .89pg/mLvs 34.0 9pg/mL ,P <0 .0 1) ;脾细胞凋亡率高于青年组 (7.7%vs 10 .3% ,P <0 .0 5 ) ,促凋亡基因Bax的转录水平也高于青年组。结论　衰老过程中脾淋巴细胞凋亡增加 ,受到相关基因的调控。

关键词：更年期大鼠血清雌二醇脾细胞增龄性改变细胞凋亡

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年轻妇女子宫肉瘤43例临床分析

复旦学报（医学版） 2003年第1期30卷 44-46,55页

作者：张建国张惜阴复旦大学附属妇产科医院妇科上海200011

目的　探讨年轻妇女子宫肉瘤的特点以及与预后有关的因素。方法　回顾性分析 1979年至 1999年我院收治的 0 .0 5 )。结论　年轻妇女的子宫肉瘤以平滑肌肉瘤及内膜间质肉瘤多见 ,临床期别比较早 ,二年、五年的存活率高于文献报道的存活... 详细信息

目的　探讨年轻妇女子宫肉瘤的特点以及与预后有关的因素。方法　回顾性分析 1979年至 1999年我院收治的 2 5例 ( 5 8% ) ,子宫内膜间质肉瘤 14例( 3 2 % ) ,子宫恶性米勒管混合瘤 4例 ( 9% )。临床分期Ⅰ期 3 4例 ,Ⅱ期 4例 ,Ⅲ期 5例。术前误诊率为 60 %。治疗以手术为主 ,辅以化疗及放疗。手术以全子宫及双 (单 )附件切除术为主 ( 70 % ) ,其次为广泛全子宫及双附件切除 +盆腔淋巴结清扫术 ( 2 3 % )。术后 3 3例 ( 76% )加用了化疗 ,3例 ( 7% )加用了放疗。结果　总的二年存活率为 86% ,五年存活率为 82 %。子宫平滑肌肉瘤及内膜间质肉瘤二年、五年存活率高于恶性米勒管混合瘤(P 0 .0 5 )。结论　年轻妇女的子宫肉瘤以平滑肌肉瘤及内膜间质肉瘤多见 ,临床期别比较早 ,二年、五年的存活率高于文献报道的存活率 ,预后相对较好。

关键词：年轻妇女子宫肉瘤临床分析预后

引物原位标记法产前诊断18号染色体数目异常

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复旦学报（医学版） 2003年第1期30卷 17-19,F003页

作者：舒群王德芬王怀军庄依亮上海市第一妇婴保健院妇产科上海200040 复旦大学附属妇产科医院产科上海200011

目的　探讨引物原位标记法 (PRINS)结合经腹脐血穿刺 ,应用于脐血中期细胞快速产前诊断 18号染色体数目异常的可行性。方法　B超监护下经腹采集胎儿脐血共 16例 ,外周血 3 4例 ,应用 18号染色体特异性引物 ,标记分裂中期细胞 18号染色体... 详细信息

目的　探讨引物原位标记法 (PRINS)结合经腹脐血穿刺 ,应用于脐血中期细胞快速产前诊断 18号染色体数目异常的可行性。方法　B超监护下经腹采集胎儿脐血共 16例 ,外周血 3 4例 ,应用 18号染色体特异性引物 ,标记分裂中期细胞 18号染色体 ,PRINS结果与中期细胞染色体G带分析结果进行比较。结果　 16例脐血中 ,PRINS检测 2例为 18三体 ,3 4例外周血中 ,1例为 18三体 ,与染色体核型分析一致。结论　PRINS是一种简便、快速、可靠的产前诊断染色体数目异常的临床检测方法。

关键词：引物原位标记法产前诊断 18三体染色体G带分析新生儿遗传病

ACTION OF BSNXD AND DHEA IN PREVENTING AND CURING POSTMENOPAUSAL OSTEOPOROSIS

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ACTION OF BSNXD AND DHEA IN PREVENTING AND CURING POSTMENOPA...

Involvement of Monocytes in Pathogenesis of Endometriosis via C-C Chemokine Pathway

The 5th International Conference on Reproductive Endocrinology

作者： Wang Yu-dong Li Da-jin Zhu Ying Laboratory for Reproductive Immunology hospital and Institute of obstetrics & gynecology shanghai medical College fudan university shanghai china.

＜正＞Objectives:To investigate the effect of Bu-shen-ning-xin （补肾宁心） decoction （BSNXD） and dehydroepiandrosterone （DHEA） on postmenopausal osteoporosis （PMOP）. Methods:Female BALB/c mice （8～9 months age） were divided randomly into six groups containing ten mice per *** of group 1 were left intact and served as a normal control group （NC group）, mice of group 2 were operated without lesion of ovarian （Sham operation group）.Mice of groups 3～6 were ovariectomized and,after a 7-day delay to allow for producing osteoporosis,were afforded with

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Involvement of Monocytes in Pathogenesis of Endometriosis vi...

CITED2基因SGJ序列插入突变与先天性心脏病发病

第六届全国免疫学学术大会

作者： Jing Yu Ying-Li Shi Da-Jin Li Laboratory for Reproductive Immunology,hospital & Institute of obstetrics and gynecology,fudan university shanghai medical College,shanghai,200011,china

The peritoneal immuno-inflammation might be associated with the origin of endometriosis, and the increased and activated pelvic macrophages and lymphocytes and the elevated levels of characteristic chemokines support ...

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复旦学报（医学版） 2008年第4期35卷 574-578页

作者：彭婷李笑天王丽复旦大学附属妇产科医院-妇产科医院研究所上海200011

目的分析先天性心脏病(congenital heart defects,CHD)患者的CITED2基因编码链基因突变的情况。方法收集101例散发型CHD患者和104例正常健康新生儿血液进行DNA抽提、PCR扩增,应用变性高效液相色谱仪进行CITED2基因全部编码序列的突变检... 详细信息

目的分析先天性心脏病(congenital heart defects,CHD)患者的CITED2基因编码链基因突变的情况。方法收集101例散发型CHD患者和104例正常健康新生儿血液进行DNA抽提、PCR扩增,应用变性高效液相色谱仪进行CITED2基因全部编码序列的突变检测,对有异常峰型的DNA进行直接测序,并与GeneBank进行比较。结果首次在动脉导管未闭的患者发现CITED2基因的一种新的插入突变,在CITED2基因编码链碱基483位起始处插入一个重复9肽(c483_484ins27),导致蛋白的丝氨酸-甘氨酸富含区(SGJ)插入9个氨基酸***161-Gly162ins9。对照组中未检测到此突变。在CITED2基因的EP300结合基序未发现突变。结论中国先心病患者中存在CITED2基因突变,新发现的CITED2基因的重复9肽插入突变c483_484ins27可能是导致动脉导管未闭发生的原因之一。

关键词：先天性心脏病 CITED2基因突变