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Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales,Phaeophyta)

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Science china(Life Sciences) 2008年第12期51卷 1116-1120页

作者： ZHANG YiChen1,2, JIANG Peng1, GAO JiangTao1,3, LIAO JianMin4, SUN ShiJing4, SHEN ZiLong4 & QIN Song1, 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, china 2 College of Chemistry and Life Science, Tianjin Normal university, Tianjin 300387, china 3 Graduate university of Chinese Academy of Sciences, Beijing 100049, china 4 Biotechnology Center, china Pharmaceutical university, nanjing 210009, china Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China College of Chemistry and Life Science Tianjin Normal University Tianjin 300387 China Graduate university of Chinese Academy of Sciences Beijing 100049 China Biotechnology Center China Pharmaceutical University Nanjing 210009 China

The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been inte-grated into the genome of gametophytes of L. japonica, and the expression product showed the ex-pected bioactivity, implying the proper post-transcript modification in haploid gametophyte.

关键词： Laminaria japonica gametophyte genetic engineering Reteplase rt-PA bioreactor

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Evidences for involvement of endogenous cAMP in Arabidopsis defens responses to Verticillium toxins

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Cell research 2005年第8期15卷 585-592页

作者： Jing JIANG, Ling Wen FAN, Wei Hua WU* The state key laboratory of Plant Physiology & Biochemistry, College of Biological Sciences, china Agricultural university, Beijing 100094, china ThestatekeylaboratoryofPlantPhysiology&Biochemistry CollegeofBiologicalSciencesChinaAgriculturalUniversity

Although there were reports suggesting the involvement of endogenous cAMP in plant defense signaling cascades, there is no direct evidence supporting this notion yet and the detailed mechanism is unclear. In the present study, we have used pathogenic fungi Verticillium dahliae and Arabidopsis plants as a model system of plant-microb interaction to demonstrate the function of endogenous cAMP in Arabidopsis defense responses. Both V. dahliae inoculation and Verticillium toxins injection induced typical “wilt” symptoms in Arabidopsis seedlings. When either 8-Br-AMP (a membrane permeable cAMP analogue) or salicylic acid (SA) was applied to Arabidopsis, the plants became resistant to V. dahliae toxins. However, addition of 8-Br-AMP did not increase the resistance of Arabidopsis transgenic plants deficient in SA to the toxins, suggesting that cAMP might act upstream of SA in plant defense signaling pathway. Indeed, 8-Br-cAMP and forskolin, an activator of adenylyl cyclase, significantly stimulated the endogenous SA level in plants, whereas DDA, an inhibitor of adenylyl cyclase dramatically reduced toxin-induced SA increase. Both the endog- enous cAMP and SA increased significantly in Arabidopsis seedlings treated with toxins. Furthermore, transcription level of pathogenesis-related protein 1 gene (PR1) was strongly induced by both 8-Br-cAMP and the toxin treatment. Taken together, our data demonstrate that endogenous cAMP is involved in plant defense responses against Verticillium- secreted toxins by regulating the production of the known signal SA in plant defense pathway.

关键词： Arabidopsis thaliana Verticillium dahliae cyclic AMP alicylic acid signal transduction defense response.

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The cessation and detoxification effect of tea filters on cigarette smoke

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Science china(Life Sciences) 2010年第5期53卷 533-541页

作者： YAN JingQi1,2,DI XiaoJing1,4,LIU CaiYi3,ZHANG HuiMin3,HUANG XiouQin3,ZHANG JunJing1,2,ZHAO Yan5,ZHANG LongZe1,CHANG YanZhong4,LIANG YongLin2$,TAO Ran3 & ZHAO BaoLu1,21 state key laboratory of Brain and Cognitive Science,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,china 2 key laboratory of Mental Health,Institute of Psychology,Chinese Academy of Sciences,Beijing 100101,china 3 Addiction Branch,Beijing Military Region General Hospital,Beijing 100700,china 4 Institute of Molecular Neurobiology,Hebei Normal university,Shijiazhuang 050016,china 5 Department of Food Sciences and Technology,Harbin Institute of Technology at Weihai,Weihai 264209,china 1. state key laboratory of Brain and Cognitive Science Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China2. Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 100101 China4. Institute of Molecular Neurobiology Hebei Normal University Shijiazhuang 050016 China3. Addiction Branch Beijing Military Region General Hospital Beijing 100700 China5. Department of Food Sciences and Technology Harbin Institute of Technology at Weihai Weihai 264209 China

To treat tobacco addiction,a tea filter was developed and studied for smoking *** work reports the smoking cessation effect of tea when it was used as a component of cigarette *** one trial it was found that after using the tea filters for 2 months,the volunteer smokers decreased their cigarette consumption by 56.5%,and 31.7% of them stopped *** work identified a new method and material,tea filter and theanine,which inhibit tobacco and nicotine addiction and provide an effective strategy for treating tobacco addiction.

关键词： cigarette smoking addiction cigarette cessation tea filter theanine nicotine dependence nicotine acetylcholine receptor(nAChR) dopamine public health free radical

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Revisit of Sheppard corrections in linear regression

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Science china Mathematics 2010年第6期53卷 1435-1451页

作者： LIU TianQing1,ZHANG BaoXue1,HU GuoRong1 & BAI ZhiDong1,2,1key laboratory for Applied Statistics of MOE and School of Mathematics and Statistics,Northeast Normal university,Changchun 130024,china 2Department of Statistics and Applied Probability,National university of Singapore,Singapore 117543,Singapore 1. key laboratory for Applied Statistics of MOE and School of Mathematics and Statistics Northeast Normal University Changchun 130024 China2. Department of Statistics and Applied Probability National University of Singapore Singapore 117543 Singapore

Dempster and Rubin(D&R) in their JRSSB paper considered the statistical error caused by data rounding in a linear regression model and compared the Sheppard correction,BRB correction and the ordinary LSE by *** asymptotic results when the rounding scale tends to 0 were also *** a previous research,we found that the ordinary sample variance of rounded data from normal populations is always inconsistent while the sample mean of rounded data is consistent if and only if the true mean is a multiple of the half rounding *** the light of these results,in this paper we further investigate the rounding errors in linear *** notice that these results form the basic reasons that the Sheppard corrections perform better than other methods in D&R examples and their conclusion in general cases is *** in which the Sheppard correction works worse than the BRB correction are also ***,we propose a new approach to estimate the parameters,called "two-stage estimator",and establish the consistency and asymptotic normality of the new estimators.

关键词： asymptotic normality consistency linear regression model rounded data two-stage estimates

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Vertical structure and seasonal variation of tidal and residual currents in the northern Huanghai Sea

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Acta Oceanologica Sinica 2011年第5期30卷 14-22页

作者： SONG Jun GUO Junru QIAO Lulu MU Lin YAO Zhigang LIU Yang LI Huan BAO Xianwen SUN Xiaoyan GAO Jia National Marine Data and Information Service State Oceanic Administration Tianjin 300171 China College of Physical and Environmental Oceanography Ocean University of China Qingdao 266100 China key laboratory of Seafloor Science and Exploration Technology Ocean University of China Qingdao 266100 China National Marine Environmental Forecasting Center State Oceanic Administration Beijing 100081 China

Analysis of tidal current and sea level has been made based on the observations from the summer of 2006 to the winter of 2007,*** result indicates that a two-layer structure of residual current exists in summer,with its upper layer going northwestward and the lower layer *** addition,some strong residuals exist in the neighboring depth of the pycnocline during the current period of astronomical *** winter,except some individual layersthe residual currents mostly direct to the northwest,from which we can see the fluctuation of abnormal sea-level and the appearance of associated current because of the changes of the wind *** analysis of tidal ellipse indicates that the direction of the maximum semidiurnal component is clockwise from summer to winter,with an angle of ***,in summer the semidiurnal component rotates with depth clockwise while the diurnal component ***,the vertical structure is almost homogeneous in winter.

关键词： northern Huanghai Sea residual current tidal wave low-pass filtering

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NUMERICAL MODELING OF WAVE EVOLUTION AND RUNUP IN SHALLOW WATER

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Journal of Hydrodynamics 2009年第6期21卷 731-738页

作者： DONG Zhi ZHAN Jie-min Department of Applied Mechanics and Engineering Sun Yat-sen University Guangzhou 510275 China Guangdong Province key laboratory of Coastal Ocean Engineering Sun Yat-sen University Guangzhou 510275China

Based on the Navier-Stokes （N-S） equations for viscous, incompressible fluid and the VOF method, 2-D and 3-D Numerical Wave Tanks （NWT） for nonlinear shallow water waves are built. The dynamic mesh technique is applied, which can save computational resources dramatically for the simulation of solitary wave propagating at a constant depth. Higher order approximation for cnoidal wave is employed to generate high quality waves. Shoaling and breaking of solitary waves over different slopes are simulated and analyzed systematically. Wave runup on structures is also investigated. The results agree very well with experimental data or analytical solutions.

关键词： VOF method shallow water waves wave evolution wave runup

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ON A CELL ENTROPY INEQUALITY OF THE RELAXINGSCHEMES FOR SCALAR CONSERVATION LAWS

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Journal of Computational Mathematics 2000年第1期18卷 69-74页

作者： Hua-zhong Tang Hua-mo Wu(state key Labomtory of Scientific and Engineering Computing, Institute of ComputationalMathematics, Chinese Academy of Sciences, Beijing 100080, china) 中科院计算数学所科学与工程计算国家重点实验室北京 100080

Presents a study which examined a cell entropy inequality for a class of local relaxation approximation relaxing scheme for scalar conservation laws. Way to obtain the scheme; Use of numerical entropy condition for th... 详细信息

关键词： hyperbolic conservation laws the relaxing schemes cell entropy inequality

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Geographical variation in reproductive traits and trade-offs between size and number of eggs in the king ratsnake,Elaphe carinata

Geographical variation in reproductive traits and trade-offs...

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第七届全国野生动物生态与资源保护学术研讨会

作者： Yan-Fu Qu~1,Hong Li~1,Jian-Fang Gao~2,Xiang Ji~1 (1.Jiangsu key laboratory for Biodiversity and Biotechnology,College of Life Sciences, nanjing Normal university,nanjing 210046,Jiangsu,china 2.Hangzhou key laboratory for Animal Adaptation and Evolution,School of Life Sciences,Hangzhou Normal university, Hangzhou 310036,Zhejiang,china)

We collected gravid king ratsnakes(Elaphe carinata) from three geographically separated populations in Chenzhou(CZ),Lishui(LS) and Dinghai(DH) of china to study geographic variation in female reproductive traits and t... 详细信息

关键词： Colubridae Life-history variation Egg-laying date Clutch size Egg size Reproductive output Egg size-number trade-off

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南海东部深海沉积物中孢粉与古环境研究

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同济大学学报（自然科学版） 2003年第5期31卷 554-557,609页

作者：张玉兰同济大学海洋地质教育部重点实验室华东师范大学河口海岸国家重点实验室上海200062

通过对南海东部 2 94站深海柱状剖面高分辨孢粉、藻类的研究 ,将 2 94站从下至上划分为 7个孢粉组合带 ,依次为 :Ⅰ带 :Dacrydium Pinus Lithocarpus Quercus(常绿 ) Cyathea Polypodiace;Ⅱ带 :Castanopsis Lithocar pus Dacrydium Qu... 详细信息

通过对南海东部 2 94站深海柱状剖面高分辨孢粉、藻类的研究 ,将 2 94站从下至上划分为 7个孢粉组合带 ,依次为 :Ⅰ带 :Dacrydium Pinus Lithocarpus Quercus(常绿 ) Cyathea Polypodiace;Ⅱ带 :Castanopsis Lithocar pus Dacrydium Quercus(常绿 ) Pinus Polypodiace ;Ⅲ带 :Quercus(常绿 ) Castanopsis Podocarpus Polypodiaceae ;Ⅳ带 :Polypodiaceae Cyathea Pteris Pinus Gramineae ;Ⅴ带 :Quercus(常绿 ) Podocarpus Lithocarpus Cyathea Polypodi aceae;Ⅵ带 :Pinus Quercus(常绿 ) Palmae Mangrovepollen Gramineae ;Ⅶ带 :Dacrydium Palmae MangrovePollen Pinus Cyperaceae Cyathea .并相应恢复了南海东部地区 12万年以来 5个植被、气候演替阶段 ,依次为 :炎热、湿润的热带季雨林—暖热而稍干的热带北缘半常绿季雨林—炎热、湿润的热带季雨林—暖热而稍干的热带北缘半常绿季雨林—炎热、湿润的热带季雨林。结合氧同位素测年资料 ,对 2 94站地层时代划分作了讨论 ,为南海古气候。

关键词：南海孢粉古环境

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基于数据挖掘的异常入侵检测系统研究

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计算机科学 2004年第10期31卷 61-65页

作者：吕志军袁卫忠仲海骏黄皓曾庆凯谢立南京大学计算机软件新技术国家重点实验室

网络上不断出现新的攻击方法,要求入侵检测系统具有能检测新的未知攻击的异常检测能力。本文提出了一个基于数据挖掘的异常入侵检测系统ADESDM。ADESDM系统提出了同时从网络数据的协议特征,端口号和应用层数据中挖掘可疑行为的方法。在... 详细信息

网络上不断出现新的攻击方法,要求入侵检测系统具有能检测新的未知攻击的异常检测能力。本文提出了一个基于数据挖掘的异常入侵检测系统ADESDM。ADESDM系统提出了同时从网络数据的协议特征,端口号和应用层数据中挖掘可疑行为的方法。在挖掘过程中,不但采用了基于强规则的关联规则挖掘方法,还针对强规则挖掘方法的缺点,提出了基于弱规则的关联规则挖掘方法,来检测那些异常操作少,分布时间长等不易检测的的网络攻击。同时利用网络通信的时间、方向、端口号、主机地址等属性之间的影响,建立以各属性为节点的贝叶斯网络作为异常判别器,进一步判别关联规则挖掘中发现的可疑行为,提高了系统检测的准确率。

关键词：入侵检测系统关联规则挖掘端口号数据挖掘异常检测网络攻击攻击方法行为影响准确率

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