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Secreted Protein HP1286 of Helicobacter pylori Strain 26695 Induces Apoptosis of AGS Cells

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Biomedical and Environmental Sciences 2012年第6期25卷 614-619页

作者： LI Jing 1,2, , MENG Fan Liang 1,2, , HE Li Hua 1,2 , and ZHANG Jian Zhong 1,2, 1. National institute for communicable disease control and prevention, chinese center for disease control and prevention, beijing 102206, china 2. state key laboratory for infectious disease prevention and control, chinese center for disease control and prevention, beijing 102206, china National institute for communicable disease control and prevention Chinese Center for Disease Control and Prevention Beijing 102206 China state key laboratory for infectious disease prevention and control Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective Secreted proteins of Helicobacter pylori （H. pylori） interact with gastric epithelium cells and may contribute to cell damage. Considering the fact that HPO17S and hypothetical conserved protein HP1286 are included in the group and that HP0175 is a well-known apoptosis-induced factor, the present study aims to clarify whether HP1286 plays a role in bacterial pathogenicity or even functions as an apoptosis-induced factor in human stomach. Methods Two genes encoding HP1286 and HPO175 were cloned into pET32a vector and expressed as recombinant proteins in Escherichia coil （E. coil） BL21. Signal peptide sequences were not included. The recombinant proteins were purified with His SpinTrap and desalted by using HiTrap Desalting. Immunoreactivity of the proteins was determined by Western blot. Human gastric epithelial cell AGS was challenged with these endotoxin-free proteins; and apoptosis of cells was assayed with the Cell Death ELISA kit. Results Recombinant proteins and their respective products whose N-terminal his-tag were removed with thrombin were recognized by serum from the patient infected with H. pylori. Apoptotic AGS cells challenged by HP1286 of H. pylori 26695 were four times more than untreated cells. In addition, apoptosis-induced ability of HP1286 of SS1 was not as strong as that of H. pylori 26695 strain. Conclusion HP1286 of H. pylori 26695 induces apoptosis of AGS cells in a time-dependent manner, however the apoptosis-induced ability of HP1286 may differ due to variations between different strains.

关键词： H. pylori HP1286 AGS Apoptosis

A Flavobacterium lindanitolerans Strain Isolated from the Ascites Sample of a chinese Patient with EV71 Virus Infection

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Biomedical and Environmental Sciences 2011年第6期24卷 694-696页

作者： TIAN Guo Zhong1,△,PIAO Dong Ri1,△,ZHAO Hong Yan1,△,JIANG Hai1,CUI Bu Yun1,and LI Jun Yan2,#1.state key laboratory for infectious diseases prevention and control,National institute for communicable disease control and prevention,chinese center for disease control and prevention,beijing 102206,china 2.National institute of Occupational Health and Poison control,chinese center for disease control and prevention,beijing 100050,china state key laboratory for infectious diseases prevention and control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China National institute of Occupational Health and Poison control Chinese Center for Disease Control and Prevention Beijing 100050 China

A strain of Flavobacterium lindanitolerans isolated from a sick child`s ascites was *** 16S rRNA gene of the strain was 100% identical to that of Flavobacterium lindanitolerans which was first identified in India in *** was first described that the isolate required X factor (Hemin) for growth in the optimal conditions of 37°C with 5% *** isolate produced indole and *** did not present hemolytic feature on blood agar.

关键词： Flavobacterium lindanitolerans 16S rRNA Biology

Interpretation Criteria for Standardized Western Blot for the Predominant Species of Borrelia Burgdorferi Sensu Lato in china

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Biomedical and Environmental Sciences 2010年第5期23卷 341-349页

作者： YI JIANG XUE-XIA HOU ZHEN GENG QIN HAO KANG-LIN WAN state key laboratory for infectious diseases prevention and control National institute of communicable disease control & prevention Chinese Center of Disease Control & Prevention Beijing 102206 China

Objective Western blotting （WB;immunoblotting） is a widely used tool for the serodiagnosis of Lyme borreliosis （LB）,but so far,no generally accepted criteria for its performance and interpretation have been established in *** present study was designed to determine the criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in china,in which WB was produced with strain PD91 as the representative strain attributed to predominant genospecies Borrelia garinii of Borrelia burgdorferi sensu *** Approximately 13 bands between 14 and 100 kD were differentiated for strain PD91 by using Gel-Pro analysis *** a study with 631 serum samples （taken from 127 patients with Lyme borreliosis and 504 controls）,all observed bands were *** establish criteria for a positive WB result for strain PD91,receiver operating characteristic （ROC） curves were *** The following interpretation criteria were recommended：for IgG,at least one band of P83/100,P58,P39,P30,OspC,P17,P66,and OspA;for IgM,at least one band of P83/100,P58,OspA,P30,OspC,P17 or *** addition,syphilis,leptospirosis and other related diseases should be excluded when the positive band is P41 in *** IgG criteria,the sensitivity is 73.2%,the specificity is 99.4% and Youden index is 0.726;for IgM criteria,the sensitivity is 50.6%,the specificity is 93.1% and Youden index is *** Standardization of WB assays is necessary for comparison of results from different ***,the criteria of other genospecies of Borrelia burgdorferi sensu lato should be determined in the future to complete the criteria of WB for the diagnosis of the Lyme disease in china.

关键词： Criteria Western blot Borrelia burgdorferi sensu lato

Genomic research for important pathogenic bacteria in china

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Science china(Life Sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 laboratory of Analytical Microbiology,state key laboratory of Pathogen and Biosecurity,beijing institute of Microbiology and Epidemiology,beijing 100071,china 2 Department of Medical Microbiology and Parasitology,institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,china 3 state key laboratory for Molecular Virology and Genetic engineering,institute of Pathogen Biology,chinese Academy of Medical Sciences,beijing 100176,china 4 state key laboratory for infectious disease prevention and control,institute for communicable disease control and prevention,chinese center for disease control and prevention,beijing 102206,china 5 beijing Genomics institute at Shenzhen,Shenzhen 518083,china laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing China Department of Medical Microbiology and Parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai China state key laboratory for Molecular Virology and Genetic engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Beijing China state key laboratory for infectious disease prevention and control Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China beijing Genomics institute at Shenzhen Shenzhen China

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

PrP mutants with different numbers of octarepeat sequences are more susceptible to the oxidative stress

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Science china(Life Sciences) 2008年第7期51卷 630-639页

作者： AN Run1 ,2,DONG ChenFang 2,LEI YanJun 1,2,HAN Lu 2,LI Ping 2,CHEN JianMing 2,WANG GuiRong 2, SHI Qi 2 ,GAO Chen 2 ,JIANG HuiYing 2 ,ZHOU Wei 2 ,HAN Jun 2 ,CHU YongLie 1 ,DONG XiaoPing 2 1School of Medicine,Xi’an JiaoTong University,Xi’an 710061,china 2state key laboratory for infectious disease prevention and control,National institute for Viral disease control and prevention, chinese center for disease control and prevention,YingXin Rd 100,beijing 100052,china School of Medicine Xi’an JiaoTong University Xi’an China state key laboratory for infectious disease prevention and control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China

One of the physiological functions of cellular prion protein(PrP C )is believed to work as a cellular resistance to oxidative stress,in which the octarepeats region within PrP plays an important ***,the detailed mechanism is less *** this study,the expressing plasmids of wild-type PrP (PrP-PG5)and various PrP mutants containing 0(PrP-PG0),9(PrP-PG9)and 12(PrP-PG12)octarepeats were generated and PrP proteins were expressed both in *** and in mammalian *** aggregation and formation of carbonyl groups were clearly seen in the recombinant PrPs expressed from *** after treatment of *** and trypan blue staining assays revealed that the cells expressing the mutated PrPs within octarepeats are less viable than the cells expressing wild-type *** significant high levels of intracellular free radicals and low levels of glutathione peroxidase were observed in the cells transfected with plasmids containing deleted or inserted *** more productions of carbonyl groups were detected in the cells expressing PrPs with deleted and inserted octarepeats after exposing to ***,cells expressing wild-type PrP showed stronger resistant activity to the challenge of H2O2 at certain extent than the mutated PrPs and mock. These data provided the evidences that the octarepeats number within PrP is critical for maintaining its activity of *** of its protective function against oxidative stress may be one of the possible pathways for the mutated PrPs to involve in the pathogenesis of familial Creutzfeldt-Jacob diseases.

关键词： prion protein oxidative stress glutathione peroxidase reactive oxygen species cell viability

Spatial prediction and analysis of Himalayan marmot plague natural epidemic foci in china based on HJ-1 satellite data

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Science china Earth Sciences 2010年第S1期53卷 8-15页

作者： GAO MengXu1,4,LI XiaoWen1,2,CAO ChunXiang1,ZHANG Hao1,LI Qun3,ZHOU Hang3 HE QiSheng1,4,XU Min1,4,ZHAO Jian1,4,ZHENG Sheng1,4 & CHEN Wei1,4 1state key laboratory of Remote Sensing Science,Jointly Sponsored by the institute of Remote Sensing Applications of chinese Academy of Sciences and beijing Normal University,beijing 100101,china 2School of Geography,beijing Normal University,beijing 100875,china 3Office for disease control and Emergency Response,chinese center for disease control and prevention,beijing 102206,china 4Graduate University of chinese Academy of Sciences,beijing 100049,china state key laboratory of Remote Sensing Science Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University Beijing China Graduate University of chinese Academy of Sciences Beijing China School of Geography Beijing Normal University Beijing China Office for disease control and Emergency Response Chinese Center for Disease Control and Prevention Beijing China

Plague,caused by the gram-negative bacterium Yersinia pestis,is a serious and rapidly progressing illness in humans that can be fatal if not treated *** Qinghai-Tibet Plateau is the largest area of natural Himalayan marmot(Marmota himalayana) plague foci in china and covers more than 630000 *** County in Gansu Province is a part of this natural focus of plague and was chosen as a study *** study used an ecological niche modeling(ENM) approach to predict the potential distribution of the Himalayan *** and Disaster Monitor Satellite(HJ-1) data was used to investigate environment factors that affect plague host animal *** animal point data from active surveillance was combined with environmental variables from the HJ-1 satellite and other databases,and the models of the potential distribution of Himalayan marmot were produced with the Genetic Algorithm for Rule-Set Production(GARP).The probability of marmot presence was divided into 0-5%,5%-20%,20%-40%,40%-80%,and 80%-100% *** with 80%-100% probability exhibited the greatest potential for the presence of Himalayan *** to the predicted potential distribution of Himalayan marmot in the study area,active surveillance of plague hosts and plague control and prevention could be more efficient.

关键词： Himalayan marmot plague spatial prediction GARP HJ-1 satellite

Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2) Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 state key laboratory for infectious disease prevention and control,National institute for Viral disease control and prevention,chinese center for disease control and prevention,beijing 100052,china 2 School of Medicine,Xi'an Jiaotong University,Xi'an 710061,china 3 National laboratory of Medical Molecular Biology,institute of Basic Medical Science,chinese Academy of Medical Sciences and Peking Union Medical College,beijing 100005,china 4 College of Science and Veterinary Medicine,Northwest Agriculture and Forest University,Yangling 712100,china state key laboratory for infectious disease prevention and control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052 China School of Medicine Xi'an Jiaotong UniversityXi'an 710061 China National laboratory of Medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005 China College of Science and Veterinary Medicine Northwest Agriculture and Forest UniversityYangling 712100 China

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 23-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

Meta-analysis of the relationship between Epstein-Barr virus infection and clinicopathological features of patients with gastric carcinoma

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Science china(Life Sciences) 2010年第4期53卷 524-530页

作者： LI ShuYing1,2, DU HaiJun3, WANG Zhan3, ZHOU Ling3, ZHAO XiaoYu1 & ZENG Yi3 1 College of Life Sciences, Hebei University, Baoding 071002, china 2 Department of Pathogenic Biology, Faculty of Biological Science, North china Coal Medical College, Tangshan 063000, china 3 National institute for Viral disease control and prevention, chinese center for disease control and prevention (chinese CDC), state key laboratory for infectious disease prevention and control (SKLID), beijing 100052, china College of Life Sciences Hebei University Baoding China Department of Pathogenic Biology Faculty of Biological Science North China Coal Medical College Tangshan China National institute for Viral disease control and prevention Chinese Center for Disease Control and Prevention (Chinese CDC) State Key Laboratory for Infectious Disease Prevention and Control (SKLID) Beijing China

Epstein-Barr virus (EBV) infection has been causally associated with occurrence of many malignant neoplasms. EBV-encoded small RNAs (EBERs) have been detected from about 10% of gastric carcinoma tissue cells, suggesting that EBV infection is associated with the development of gastric carcinoma. The present study pooled the data from the papers concerning EBV-related gastric cancers and performed a meta-analysis of 22 research papers. Among these papers, a total of 5475 cases with gastric cancer were enrolled, of whom 411 cases were found EBV-positive, with the EBV-positive rate being 7.5%. Among the EBV-positive gastric cancer cases, the detection rate was 11.1% in males and 3.0% in females. Compared with EBV-negative gastric cancer, EBV-positive gastric cancer had less lymph node metastasis. Based on the histological typing, of the EBV-positive gastric cancers, the diffuse type was 8.1%, and intestinal type was 8.0%. The examined specimen types included stored paraffin blocks and fresh surgically removed specimens, their EBV positive rates were 7.9% and 6.5% respectively. In terms of geographical distribution, the detection rate of EBV-positive gastric cancer was 9.4% in America, 6.1% in Asia and 9.1% in Europe. Meta-analysis showed that EBV infection occurred only in gastric cancer tissue cells and was significantly associated with the patients’ gender, lymph node metastases, and the location where tumor tissue generated and geographical distribution (P0.05). These results suggested that EBV-positive gastric cancer has distinct clinicopathological features.

关键词： Epstein-Barr virus gastric carcinoma meta-analysis in situ hybridization

Characterization of serogroup C meningococci isolated from 14 provinces of china during 1966—2005 using comparative genomic hybridization

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Science china(Life Sciences) 2007年第1期50卷 1-6页

作者： PENG JunPing1, ZHANG XiaoBing1, YANG E1, WANG Jing1, YANG Jian1, SHAO ZhuJun2, 3 & JIN Qi1 1 state key laboratory for Molecular Virology and Genetic Engineering, beijing 100052, china 2 National institute for communicable disease control and prevention, beijing 102206, china 3 state key laboratory for infectious disease prevention and control, beijing 102206, china state key laboratory for Molecular Virology and Genetic Engineering Beijing 100052 China National institute for communicable disease control and prevention Beijing 102206 China state key laboratory for infectious disease prevention and control Beijing 102206 China

Neisseria meningitidis is a major cause of bacterial meningitis and septicemia worldwide. In china, serogroup A strains were responsible for over 95% of the cases, while serogroup B strains were mainly the cause of localized outbreaks and sporadic cases. Before 2003, serogroup C strains were only re-covered from a few sporadic cases. However, a sudden increase in the number of cases due to sero-group C strains occurred during 2003—2005 in Anhui Province, china. Many cases were found in other provinces at the same time. Multilocus sequence typing (MLST) results indicated that the unique se-quence type 4821 clone meningococci, a new hyper-virulent lineage, was responsible for the serogroup C meningitis outbreaks. We have completed the project of sequencing the whole genome of the Chi-nese N. meningitidis serogroup C representative isolate 053442. We fabricated a whole-genome mi-croarray of N. meningitidis isolate 053442 and analyzed the genome composition differences among 81 serogroup C isolates which were isolated from 14 provinces of china during 1966—2005. The com-parative genomic hybridization (CGH) result shows that the genome compositions of nearly all sero-group C isolates are similar to that of 053442. The products of many absent open reading frames (ORFs) are conserved hypothetical proteins. The results will provide a valuable resource from which one can analyze the genome composition and genetic background of serogroup C meningococci in china.

关键词： Neisseria meningitidis comparative genomic hybridization ST-4821