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Sequence analysis of mRNA polyadenylation signals of rice genes

chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2, GAO Chenxi1,2,3 & HAN Bin1,3,41. National Center for Gene research, shanghai institutes for Biological sciences, chinese academy of sciences, shanghai 200233, china 2. Graduate School of the chinese academy of sciences, Beijing 100039, china 3. institute of Plant Physiology and Ecology, shanghai institutes for Biological sciences, chinese academy of sciences, shanghai 200032, china National Center for Gene research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China Graduate School of the chinese academy of sciences Beijing 100039 China institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3′-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12969 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953 genes. The al- ternative poly(A) sites were revealed in approxi- mately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3′ fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3′ fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

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Continuous wavelet transform and discrete multi-resolution analysis of surface fluxes and atmospheric stability

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Progress in Natural Science:Materials International 2006年第4期16卷 403-409页

作者： JIANG Zhaoyang, YU Qiang, WANG Tianduo, SUN Xiaomin and ZHANG Renhua shanghai institute of Plant Physiology and Ecology, chinese academy of sciences, shanghai 200032, china institute of Geographic sciences and Natural Resources research, chinese academy of sciences, Beijing 100101, china [a]shanghai institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China [b]institute of Geograhpic sciences and Natural Resources research Chinese Academy of Sciences Beijing China

Variations of land surface fluxes of sensible heat (H), latent heat (LE), and CO2(F-CO2) obtained from the eddy-co-variance measurements above a winter wheat field from March 30 to April 24, 2001 have been studied at scales ranging from 10 minutes to days. Wavelet transform is used in the analysis of land surface fluxes and atmospheric stability (ξ) calculated from the measurements to reveal the changes in land surface fluxes in hours to days scales. The main results are: (1) Concise and compact information about the fluxes, net radiation (Rn), temperature ( T) and ξ in the scale-time domain are extracted from the data by continuous wavelet analysis, and 1 day, 0.5 day and short-period (shorter than 0.5 day) components are revealed. Continuous wavelet coefficients can be used to characterize periodic components of changes in fluxes and ξ. (2) Discrete-time multi-resolution analysis can be used to concentrate total energy variance of time series of the measurements to a small number of coefficients, plotting the relative energy distribution to get several meaningful characteristics of the data. (3) Under neutral atmospheric conditions, the relative energy distributions of the Haar multi-resolution analysis of the three non-dimensional coefficients ( T/T* , q/q * and c/c * ) display clear similarities.

关键词： atmospheric stability flux continuous wavelet transform multi-resolution wavelet analysis.

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RADIAL BASIS FUNCTION INTERPOLATION IN SOBOLEV SPACES AND ITS APPLICATIONS

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Journal of Computational Mathematics 2007年第2期25卷 201-210页

作者： Manping Zhang (School of Mathematical sciences,Fudan University,shanghai 200433,china institute of Computational Mathematics,chinese academy of sciences,Beijing 100080,china School of Mathematical sciences Fudan University Shanghai 200433 China institute of Computational Mathematics Chinese Academy of Sciences Beijing 100080 China

In this paper we study the method of interpolation by radial basis functions and give some error estimates in Sobolev space H^k（Ω）（k 〉 1）. With a special kind of radial basis function, we construct a basis in H^k（Ω） and derive a meshless method for solving elliptic partial differential equations. We also propose a method for computing the global data density.

关键词： Sobolev space Radial basis function Global data density Meshless method

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Study on animals' femur blood flow alteration under inadequate decompression with ^(133)Xe isotope washout method

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nuclear Science and Techniques 1998年第2期9卷 124-128页

作者： Yu Shao-Ning Bao Bo-Rong (shanghai institute of nuclear research, the chinese academy of sciences, shanghai 201800 )Tian Wu-Xun Yun Chang-Quan Zhu Xiang-Qi Wang Jing-Ying and Cao Bao-Gen (Naval Medical research institute, shanghai 200433) shanghai institute of nuclear research Chinese Academy of Sciences Shanghai 201800 China Naval Medical research institute Shanghai 200433 China

Animals’ regional femur blood (F) alteration was studied under an inadequate decompression by using the inhaled isotope 133Xe washout method. Results showed that the average F on left and right side of minipigs’ femur decreased from 15.4±1.8 and 16 .9±2.0 ml/ (100 ***) (before exposure) to 10±1.8 and 11.1±1.6 ml/(***) (after expposure for 1.5 h under the pressure of 0.5 MPa and then decompression to normal with the rate of 0.03~0.04MPa/min), respectively; the blood flow of hematopoietic marrow tissues (f1)on both sides also decreased from 19.3±2.0 and 22.1±1.9 ml/(***) to 13.9±1.4 and 13.8±1.0 ml/(100 ***). The exact same alteration also happened in the experiment on New Zealand rabbits. It indicates that inadequate decompression in hyperbaric exposure can give rise to decrease animals’ bone blood flow and insufficient bone blood supply serves as one of the reasons for causing decompressive osteonecrosis.

关键词： 133Xe同位素动物股骨血液流减压

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Production and measurement of Bose-Einstein condensate of ^(87)Rb atomic gas

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Science china(Physics,Mechanics & Astronomy) 2008年第1期51卷 22-28页

作者： XU Zhen ,ZHOU ShuYu,ZHOU ShanYu&WANG YuZhu Key Laboratory for Quantum Optics,shanghai institute of Optics and Fine Mechanics,chinese academy of sciences,shanghai 201800,china 1. Key Laboratory for Quantum Optics Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China

The research platform for Bose-Einstein condensate in 87 Rb atomic gas,which is composed of a double MOT configuration and a QUIC trap,was *** properties of the condensate were measured both in time-of-flight and in tight confinement by the absorption imaging *** measurements agreed with the criterions of Bose-Einstein condensation phase *** 2×10 5 atoms were pure condensed.

关键词： laser cooling and trapping Bose-Einstein condensate time-of-flight measurement

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Bone mineral density of normal people by dual energy X-ray absorptiometry

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nuclear Science and Techniques 1997年第4期8卷 246-248页

作者： Zhang Yuan Xun, Li De Yi ( shanghai institute of nuclear research, the chinese academy of sciences, shanghai 201800) Ma Ji Xiao and Huang Qi Ren ( shanghai Sixth People’s Hospital, shanghai 200233 )

ＢｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙｏｆｎｏｒｍａｌｐｅｏｐｌｅｂｙｄｕａｌｅｎｅｒｇｙＸｒａｙａｂｓｏｒｐｔｉｏｍｅｔｒｙＺｈａｎｇＹｕａｎＸｕｎ，ＬｉＤｅＹｉ（ＳｈａｎｇｈａｉＩｎｓｔｉｔｕｔｅｏｆＮｕｃ... 详细信息

ＢｏｎｅｍｉｎｅｒａｌｄｅｎｓｉｔｙｏｆｎｏｒｍａｌｐｅｏｐｌｅｂｙｄｕａｌｅｎｅｒｇｙＸｒａｙａｂｓｏｒｐｔｉｏｍｅｔｒｙＺｈａｎｇＹｕａｎＸｕｎ，ＬｉＤｅＹｉ（ＳｈａｎｇｈａｉＩｎｓｔｉｔｕｔｅｏｆＮｕｃｌｅａｒＲｅｓｅａｒｃｈ，ｔｈｅＣ...

关键词： X射线吸收测量学骨矿物质密度年龄关系

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The relation between black hole masses and Lorentz factors of the jet components in blazars

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research in Astronomy and Astrophysics 2009年第3期9卷 293-301页

作者： Ming Zhou1,2,3 and Xin-Wu Cao1,2 1 shanghai Astronomical Observatory, chinese academy of sciences, shanghai 200030, china 2 Joint institute for Galaxy and Cosmology (JOINGC) of SHAO and USMC, shanghai 200030, china 3 Graduate School of the chinese academy of sciences, Beijing 100049, china shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Joint institute for Galaxy and Cosmology (JOINGC) of SHAO and USMC Shanghai 200030 China Graduate School of the chinese academy of sciences Beijing 100049 China

We explore the relationship between black hole mass (MBH) and the motion of the jet components for a sample of blazars. The Very Long Baseline Array (VLBA) 2 cm Survey and its continuation: Monitoring of Jets in active galactic nuclei (AGNs) with VLBA Experiments (MOJAVE) have observed 278 radio-loud AGNs, of which 146 blazars have reliable measurements of their apparent velocities of the jet components. We calculate the minimal Lorentz factors for these sources from their measured apparent velocities, and their black hole masses are estimated with their broad-line widths. A sig- nificant intrinsic correlation is found between black hole masses and the minimal Lorentz factors of the jet components. The Eddington ratio is only weakly correlated with the min- imal Lorentz factor, which may imply that the Blandford-Znajek (BZ) mechanism may dominate over the Blandford-Payne (BP) mechanism for the jet acceleration (at least) in blazars.

关键词： black hole physics — galaxies: active — galaxies: jets — galaxies: nuclei

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Temporal Pulse Shaping of Laser Beams by Optical-Fiber Stacker

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chinese Journal of Lasers 1999年第1期8卷 46-49页

作者： WANG Chun XU Shixiang CHEN Shaohe DENG Ximing LIN Zunqi FAN Dianyuan (shanghai institute of Optics and Fine Mechanics, The chinese academy of sciences, shanghai 201800, china) 中科院上海光学精密机械所上海 201800 中科院上海光学精密机械所上海

１ＩｎｔｒｏｄｕｃｔｉｏｎＰｒｅｃｉｓｅｔｅｍｐｏｒａｌｐｕｌｓｅｓｈａｐｉｎｇｏｆｔｈｅｌａｓｅｒｂｅａｍｓｆｏｒｉｎｅｒｔｉａｌｃｏｎｆｉｎｅｍｅｎｔｆｕｓｉｏｎ（ＩＣＦ）ｅｘｐｅｒｉｍｅｎｔｓｈａｓｂｅｃｏｍ... 详细信息

１ＩｎｔｒｏｄｕｃｔｉｏｎＰｒｅｃｉｓｅｔｅｍｐｏｒａｌｐｕｌｓｅｓｈａｐｉｎｇｏｆｔｈｅｌａｓｅｒｂｅａｍｓｆｏｒｉｎｅｒｔｉａｌｃｏｎｆｉｎｅｍｅｎｔｆｕｓｉｏｎ（ＩＣＦ）ｅｘｐｅｒｉｍｅｎｔｓｈａｓｂｅｃｏｍｅｉｎｃｒｅａｓｉｎｇｉｍｐｏｒｔ...

关键词： inertial confinement fusion pulse shaping optical fiber stacker

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The background of the total synthesis of yeast alanine transfer RNA

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Science china(Life sciences) 2010年第1期53卷 19-21页

作者： QI GuoRong institute of Biochemistry and Cell Biology,shanghai institute for Biological sciences,chinese academy of sciences,shanghai 200031,china 1. institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

The research findings concerning the total synthesis of yeast alanine transfer RNA (yeast alanine tRNA) were successively published in chinese Science Bulletin (1982) and Science

关键词： tRNA The background of the total synthesis of yeast alanine transfer RNA

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The cDNA and Genomic DNA Organization of a Novel Toxin SHT-I from Spider Ornithoctonus huwena

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 656-660页

作者： Peng QIAO, Xiao-Pan ZUO, Zhi-Fang CHAI, and Yong-Hua JI* institute of Physiology, shanghai institutes for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, shanghai 200031, china institute of Physiology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences

In present study the full-length cDNA of a novel toxin from the venom gland of spider Ornithoctonus huwena, named as SHT-I, has been cloned using the strategy of rapid amplification of cDNA ends, and then the whole genomic sequence of SHT-I (Selenocosmia huwena toxin-I) was determined using sequence-specific primers synthesized based on the acquired 3’ and 5’ ends of SHT-I cDNA sequence. It is unexpectedly found that intron was lacking in the genomic sequence of SHT-I. The result might evoke an interesting question whether the gene code of inhibitor cystine-knot peptides from spider venom is distinct from that of those known toxic peptides of scorpion and cone snail.

关键词： Ornithoctonus huwena spider cDNA genomic DNA peptide toxin ICK motif

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