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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese academy of sciences, Beijing 100080, china 2center of Mass Spectrometry, Institute of Chemistry, chinese academy of sciences, Beijing 100080, china 3Graduate School of the chinese academy of sciences, Beijing 100039, china Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China Graduate School of the chinese academy of sciencesBeijing 100039 China center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

Temporal variation of hemispheric solar rotation

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Research in Astronomy and Astrophysics 2012年第2期12卷 187-200页

作者： Jing-Lan Xie 1,2,Xiang-Jun Shi 1,2 and Jing-Chen Xu 1,21 national Astronomical Observatories /Yunnan Observatory,chinese academy of sciences,Kunming 650011,china 2 Graduate University of chinese academy of sciences,Beijing 100049,china national Astronomical Observatories/ Yunnan Observatory Chinese Academy of Sciences Kunming 650011 China Graduate University of chinese academy of sciences Beijing 100049 China

The daily sunspot numbers of the whole disk as well as the northern and southern hemispheres from 1945 January 1 to 2010 December 31 are used to investi- gate the temporal variation of rotational cycle length through the continuous wavelet transformation analysis method. Auto-correlation function analysis of daily hemi- spheric sunspot numbers shows that the southern hemisphere rotates faster than the northern hemisphere. The results obtained from the wavelet transformation analysis are that no direct relationship exists between the variation trend of the rotational cy- cle length and the solar activity in the two hemispheres and that the rotational cycle length of both hemispheres has no significant period appearing at 11 yr, but has a sig- nificant period of about 7.6 yr. Analysis concerning the solar cycle dependence of the rotational cycle length shows that acceleration seems to appear before the minimum time of solar activity in the whole disk and the northern hemisphere, respectively. Furthermore, the cross-correlation study indicates that the rotational cycle length of the two hemispheres has different phases, and that the rotational cycle length of the whole disk as well as the northern and southern hemispheres, also has phase shifts with corresponding solar activity. In addition, the temporal variation of the north-south （N- S） asymmetry of the rotational cycle length is also studied. This displays the same variation trend as the N-S asymmetry of solar activity in a solar cycle, as well as in the considered time interval, and has two significant periods of 7.7 and 17.5 yr. Moreover, the rotational cycle length and the N-S asymmetry of solar activity are highly corre- lated. It is inferred that the northern hemisphere should rotate faster at the beginning of solar cycle 24.

关键词： Sun: activity - Sun: rotation - Sun: sunspot

A method to predict amplitude and date of maximum sunspot number

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chinese Science Bulletin 2000年第14期45卷 1287-1290页

作者： HAN YanbenBeijing Astronomical Observatory, chinese academy of sciences, national Astronomical Observatories, chinese academy of sciences, Beijing 100012, china Beijing Astronomical Observatory Chinese Academy of Sciences National Astronomical Observatories Chinese Academy of Sciences Beijing China

A method to predict the amplitude and date of the maximum sunspot number is introduced. The regression analysis of the relationship between the variation rate of monthly sunspot numbers in the initial stage of solar cycles and both of the maximum and the time-length of ascending period of the cycle showed that they are closely correlative. In general, the maximum will be larger and the ascending period will be shorter when the rate is larger. The rate of sunspot numbers in the initial 2 years of the 23rd cycle is thus analyzed based on these grounds and the maximum of the cycle is predicted. For the smoothed monthly sunspot numbers, the maximum will be about 139.2 ±18.8 and the time-length of ascending period will be about 3.31 ±0.42 years, that is to say, the maximum will appear around the spring of the year 2000. For the mean monthly ones, the maximum will be near 170.1±22.9 and the time-length of ascending period will be about 3.42 ±0.46 years, that is to say, the appearing date of the maximum

关键词： solar activity prediction sunspot number 23rd solar cycle.

Direct Optimization of Low-thrust Many-revolution Earth-orbit Transfers

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chinese Journal of Aeronautics 2009年第4期22卷 426-433页

作者： Gao Yang academy of Opto-Electronics,chinese academy of sciences,Beijing 100190,china [a]academy of Opto-Electronics Chinese Academy of Sciences Beijing 100190 China

Low-thrust Earth-orbit transfers with 10^- 5-order thrust-to-weight ratios involve a large number of orbital revolutions which poses a real challenge to trajectory optimization. This article develops a direct method to optimize minimum-time low-thrust many-revolution Earth-orbit transfers. A parameterized control law in each orbit, in the form of the true optimal control, is proposed, and the time history of the parameters governing the control law is interpolated through a finite number of nodal values. The orbital averaging method is used to significantly reduce the computational workload and the trajectory optimization is conducted based on the orbital averaging dynamics expressed by nonsingular equinoctial elements. Furthermore, Earth＇s shadowing and perturbation effects are taken into account. The optimal transfer problem is thus converted to the parameter optimization problem that can be solved by nonlinear programming. Taking advantage of the mapping between the parameterized control law and the Lyapunov control law, a technique is proposed to acquire good initial guesses for optimization variables, which results in enlarged convergence domain of the direct optimization method. Numerical examples of optimal Earth-orbit transfers are presented.

关键词： low thrust orbital transfer optimization direct method orbital averaging Lyapunov method

Dynamics of morphological changes for mitochondrial fission and fusion

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Science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 Key Laboratory of Microgravity (national Microgravity Laboratory), Beijing 100190, china 2 center for Biomechanics and Bioengineering, Institute of Mechanics, chinese academy of sciences, Beijing 100190, china 3 State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, chinese academy of sciences, Beijing 100101, china 4 Institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, Beijing 100044, china 1. Key Laboratory of Microgravity (national Microgravity Laboratory) Beijing 100190 China2. Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China4. Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing 100044 China3. State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

Drifting bands of solar radio spikes

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chinese Science Bulletin 2008年第4期53卷 638-640页

作者： XU FuYing1,2& WU HongAo1,2 1 Purple Mountain Observatory,chinese academy of sciences,Nanjing 210008,china 2 national Astronomical Observatories,chinese academy of sciences,Beijing 100012,china Purple Mountain Observatory Chinese Academy of Sciences Nanjing China national Astronomical Observatories Chinese Academy of Sciences Beijing China

The solar flare(1N/C5.3) occurred in NOAA 8742 on October 27,1999,and the accompanied complex radio burst was observed by the spectrometer with both high temporal(5 ms) and frequency(10 MHz) resolutions at Purple Mountain Observatory(PMO).It started at 04:27:42 UT,peaked at 04:28:42 UT and ended at 04:29:18 *** most significant feature of the event might be the sequential spikes in both time and frequency to form three slow drift bands over the frequency range of ～5 to ≥7.5 GHz with almost the same drift rate about 2.7 GHz/*** relative bandwidth for the individual spikes varied from 1.6% to 4.9%,typically 3.3%.Based on electron cyclotron maser action driven by loss-cone anisotropy,the rare phenomena of slow drift band consisting of spikes were interpreted by spike source moving back and forth along the magnetic ***,the estimations of the narrow bandwidths of spike given by electron cyclotron maser theory coincided well with the observations.

关键词：太阳耀斑微波爆裂太阳活动天文学

An Alternative Explanation for a Screw-like Meteoric Train Photographed by Double-Station Observations

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chinese Journal of Astronomy and Astrophysics 2007年第6期7卷 814-822页

作者： Guang-Jie Wu1,2 1 national Astronomical Observatories/Yunnan Observatory,chinese academy of sciences,Kunming 650011 2 Purple Mountain Observatory,chinese academy of sciences,Nanjing 210008 Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008

Two-station observation of meteors, especially a meteor trains, provides an effective approach to the measurement of the physical parameters. We have collected four special groups of photographs of meteoric trains taken at two stations during Leonids 2001. One representative group has been measured and analyzed in detail. An analysis has been reported in our first paper. In this paper, an alternative explanation for the screw-like meteoric train is suggested based on some physical calculations. The results reveal that this train has a screw-like structure and, apparently, spoke beams. The mother meteor of this train may be negatively charged and moves forward along a left-hand screw trajectory under the effect of the geomagnetic field. The spoke beams might be the visual effect of the long time exposure of many particles released from the disintegrated meteoroid.

关键词： meteors meteoroids -- magnetic fields

Nonlinear interactions between gravity waves and background winds

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Progress in Natural Science:Materials International 2007年第6期17卷 639-644页

作者： Liu Xiao1,2 and Xu Jiyao1(1. State Key Laboratory for Space Weather, center for Space Science and Applied Research, chinese academy of sciences, Beijing 100080, china 2. Graduate University of chinese academy of sciences, Beijing 100039, china) [a]State Key Laboratory for Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China [b]Graduate University of chinese academy of sciences Beijing China

Using the nonlinear propagating gravity waves (GW) model in the two-dimensional compressible atmosphere and the linear GW theory, the process of GW propagation in different background winds, e.g. the direction of the background wind is opposite to (dead wind) or the same as (tail wind) the direction of the horizontal phase velocity of GW, is studied. The results show that the dead wind prolongs the vertical wavelength and accelerates GW propagation. Therefore, GW propagates up to a higher height becomes instable in a short time and eventually induces an inverse jet flow. Then, the vertical wavelength is becoming short due to the nonlinear interactions between GW and the inverse jet flow. The vertical wavelength and group velocity decrease after GW propagates into the tail wind. The initial instable time is delayed. Although most of GW is trapped in the instable region, some of GW propagates above the instable region. Compared with GW propagation in the tail wind, the nonlinear interactions between GW and the dead wind are also strong. In contrast, the linear GW theory predicts that GW can propagate freely in the dead wind. The vertical wavelength simulated by the nonlinear numerical model is different from that predicted by the linear theory greatly after GW propagates into the dead wind.

关键词： gravity waves background winds vertical group velocity vertical wavelength nonlinear interactions.

Monaural voiced speech segregation based on elaborate harmonic grouping strategies

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Science china(Information sciences) 2011年第12期54卷 2491-2500页

作者： LIU WenJu 1 , ZHANG XueLiang 1 , JIANG Wei 1 , LI Peng 2 & XU Bo 2 1 national Laboratory of Pattern Recognition, Institute of Automation, chinese academy of sciences, Beijing 100190, china 2 Digital Media Content Technology Research center, Institute of Automation, chinese academy of sciences, Beijing 100190, china 1. national Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing 100190 China2. Digital Media Content Technology Research Center Institute of Automation Chinese Academy of Sciences Beijing 100190 China

In this paper, an enhanced algorithm based on several elaborate harmonic grouping strategies for monaural voiced speech segregation is proposed. Main achievements of the proposed algorithm lie in three aspects. Firstly, the algorithm classifies the time-frequency (T-F) units into resolved and unresolved ones by carrier-to-envelope energy ratio, which leads to more accurate classification results than by cross-channel correlation. Secondly, resolved T-F units are grouped together according to minimum amplitude principle, which has been verified to exist in human perception, as well as the harmonic principle. Finally, "enhanced" envelope autocorrelation function is employed to detect amplitude modulation rates, which helps a lot in reducing half-frequency error in grouping of unresolved units. Systematic evaluation and comparison show that performance of separation is greatly improved by the proposed algorithm. Specifically, signal-to-noise ratio (SNR) is improved by 0.96 dB compared with that of previous method. Besides, our algorithm is also effective in improving the PESQ score and subjective perception score.

关键词： computational auditory scene analysis voiced speech separation harmonistic principle minimum amplitude principle elaborate harmonic grouping strategies