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Cell Research 2006年第3期16卷 313-318页

作者： Yanyong Kang~(1,3) Fengcai Wang~(1,2) Jing Feng~1 Dongling Yang~1 Xu Yang~3 Xiyun Yan~11 national Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of science beijing 100101,china 2 Chemistry Department,Anyang teacher’s college,Anyang 455000,china 3 college of life science,central china Normal university,Wuhan 430079,china national Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Science Beijing China college of life science Central China Normal University Wuhan China Chemistry Department Anyang teacher's College Anyang China

Our previous study has demonstrated that CD 146 molecule is a biomarker on vascular endothelium,which is involvedin angiogenesis and tumor growth.However the mechanism behind is not clear.Here we have for the first time devel-oped a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells.Our data showedthat CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels,leading to thesignificant suppression of HUVEC proliferation,adhesion and migration.These results demonstrate that CD146 playsa key role in vascular endothelial cell activity and angiogenesis,and CD146 siRNA can be used as a new inhibitor foranti-angiogenesis therapy.

关键词： small interfering RNA adhesion molecule CD 146 vascular endothelial cell

Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses

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science china(life sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2, SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research Institute/national Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural science, beijing 100081, china 2college of life and environmental sciences, Minzu university of china, beijing 100081, china Biotechnology Research Institute/national Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Science Beijing China college of life and environmental sciences Minzu University of China Beijing China

The protein kinase AtMPK3,a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3 is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3 gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3 promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3 promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-62 upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3 promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress

Molecular cloning and functional analysis of Chinese sturgeon (Acipenser sinensis) growth hormone receptor

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science china(life sciences) 2009年第10期52卷 911-921页

作者： LIAO ZhiYong1, CHEN XiaoLi2 & WU MingJiang1 1 college of life and environmental science, Wenzhou university, Wenzhou 325035, china 2 Capital Institute of Pediatrics, beijing 100020, china college of life and environmental science Wenzhou University Wenzhou China Capital Institute of Pediatrics Beijing China

A full length cDNA encoding the growth hormone receptor (GHR) of Chinese sturgeon was cloned in order to investigate the mechanism of growth hormone in regulating the growth of Chinese sturgeon. The open reading frame of the cloned Chinese sturgeon growth hormone receptor (csGHR) cDNA encodes a trans-membrane protein of 611 amino acids containing all the characteristic motifs of GHR. By sequence alignment, substitutions of amino acid residues highly conserved in other species were identified. Using the CHO cell culture system, the function of csGHR and the biological significance of the amino acid substitution in csGHR were examined. The promoter of serine protease inhibitor 2.1 (Spi2.1) was trans-activated upon stimulation of seabream GH (sbGH) in the csGHR-expressing CHO cells. Furthermore, CHO cells stably expressing csGHR were stimulated to proliferate by sbGH. In agreement with our previous report, Chinese sturgeon growth hormone-binding protein (csGHBP) was detected in the culture medium of CHO cells stably expressing csGHR. Mutation of Asp residue in the ligand binding motif in csGHR to Glu significantly enhanced csGHR's biological function, whereas mutation of Asp residue to Ala decreased its biological function. The results demonstrated that the cloned csGHR was of full biological function and the csGHBP could be generated through proteolysis of csGHR. These findings might provide new insights into thoroughly understanding the regulatory mechanism of Chinese sturgeon growth.

关键词： Chinese sturgeon GHR GHBP mutation cDNA cloning

Pelagodictyon Clarke,a newly recorded nanodiatom genus for china

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Journal of Systematics and Evolution 2009年第2期47卷 179-182页

作者： 1,2Yang LI 2Ya-Hui GAO 1(Key Laboratory of Ecology and environmental science in Guangdong Higher Education,college of life science,South china Normal university,Guang-zhou 510631,china) 2(School of life sciences,Xiamen university,Xiamen 361005,china) Key Laboratory of Ecology and environmental science in Guangdong Higher Education College of Life Science South China Normal University Guangzhou 510631 China School of life sciences Xiamen University Xiamen 361005 China

Marine nanoplanktonic diatoms have been frequently either underestimated or overlooked in phyto-plankton investigations due to their small size(2-20 μm),which allows most of them to pass through a regular phytoplankton net of 70-μm pore size.For nanoplanktonic diatom classification,water samples(each 2000 mL) were collected from several representative sea areas of Chinese coast from May 2002 to May 2006.Pelagodictyon Clarke was identified as a newly recorded nanodiatom genus for china under transmission electron microscope(TEM).This genus is characterized by the delicate structure of the valve face.The striae are seen to be formed by ribs with cross-ribs(frets) and the intervening spaces are occupied by polygonal cribra situated much close to the exterior face of the valve.Just one species was found,i.e.,P.tenue Clarke.Detailed description of the taxonomic characteristics with TEM photographs of this species and its ecological habitat and distribution are given in this paper.Morphological characteristics comparisons among several resembling genera were discussed.

关键词： china fret nanodiatom new record Pelagodictyon Stephanodiscaceae

Effects of chronic exposure to nonylphenol on locomotor activity and social behavior in zebrafish(Danio rerio)

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Journal of environmental sciences 2010年第9期22卷 1435-1440页

作者： Jigang Xia,Cuijuan Niu ,Xuejiao Pei Ministry of Education Key Laboratory of Biodiversity science and Ecological Engineering,college of life sciences,beijing Normal university,100875 beijing,china. Ministry of Education Key Laboratory of Biodiversity science and Ecological Engineering College of Life Sciences Beijing Normal University 100875 Beijing China

Nonylphenol （NP）, a xenoestrogen ubiquitously found in aquatic ecosystems, is of high environmental concern. The present work assessed the effects of exposure to NP on locomotor activity and social behavior in male and female zebrafish （Danio rerio）, to evaluate the possible hazard of NP to fish behavior and to pursue a potential biomarker of NP contamination. Fish were randomly divided into six groups. Five groups were receiving 0.1, 1, 10, 50 and 100 μg/L nominal concentrations of NP for 60 days, respectively. A sixth control group was given the same treatment as the other five groups, but no NP. Locomotor activity, aggressive behavior, group preference and leaving shoal were examined. NP exposure showed marked influence on locomotor activity of the male zebrafish, whereas that of the female was not significantly affected by NP. Aggressive behavior and group preference were significantly affected by NP exposure in both male and female groups. Locomotor activity and aggressive behavior of the male and group preference of both male and female zebrafish were clearly inhibited at 100 μg/L NP. No significant alteration in leaving shoal was observed under NP exposure. The results suggested that changes in locomotor activity and aggressive behavior of the male and group preference of both male and female may be used as an ecologically relevant integrative biomarker of NP contamination.

关键词： chronic toxicity nonylphenol biomarker zebrafish

Use of the modified viral satellite DNA vector to silence mineral nutrition-related genes in plants: silencing of the tomato ferric chelate reductase gene, FRO1, as an example

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science china(life sciences) 2008年第5期51卷 402-409页

作者： HE XiuXia1,2,JIN ChongWei1, LI GuiXin3, YOU GuangYi4, ZHOU XuePing3 & ZHENG ShaoJian4 1 Key Laboratory for environmental and Ecosystem Health of the Ministry of Education, college of environmental and Resource science, Zhejiang university, Hangzhou 310029, china 2 college of life science, Changchun university of science and Technology, Changchun 130022, china 3 Institute of Biotechnology, Zhejiang university, Hangzhou 310029, china 4 State Key Laboratory of Plant Physiology and Biochemistry, college of life science, Zhejiang university, Hangzhou 310058, china Key Laboratory for environmental and Ecosystem Health of the Ministry of Education College of Environmental and Resource Science Zhejiang University Hangzhou China college of life science Changchun University of Science and Technology Changchun China Institute of Biotechnology Zhejiang University Hangzhou China State Key Laboratory of Plant Physiology and Biochemistry College of Life Science Zhejiang University Hangzhou China

Virus-induced gene silencing (VIGS) is potentially an attractive reverse-genetics tool for studies of plant gene function, but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. Here we report on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmβ) associated with Tomato yellow leaf curl china virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FRO1) from tomato was inserted into the DNAmβ vector. Tomato roots agro-inoculated with DNAmβ carrying both a fragment of FRO1 and TYLCCNV used as a helper virus exhibited a significant reduction at the FRO1 mRNA level. As a consequence, ferric chelate reductase activity, as determined by visualization of the pink FeBPDS3 complex was significantly decreased. Our results clearly demonstrated that VIGS system can be employed to investigate gene function associated with plant nutrient uptake in roots.

关键词： virus-induced gene silencing roots ferric chelate reductase

Scenario simulation and forecast of land use/cover in northern china

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Chinese science Bulletin 2008年第9期53卷 1401-1412页

作者： LI YueChen 1,2,3 & HE ChunYang 3 1 college of Geographical science, Chongqing Normal university, Chongqing 400047, china 2 college of Resources and environmental science, Chongqing university, Chongqing 400044, china 3 college of Resources science & Technology, beijing Normal university, beijing 100875, china college of Geographical science Chongqing Normal University Chongqing China college of Resources and environmental science Chongqing University Chongqing China college of Resources science & Technology Beijing Normal University Beijing China

Modeling land use/cover scenario changes and its potential impacts on structure and functions of ecosystem in typical regions are helpful to understanding the interactive mechanism between land use/cover system and ecosystem. Based on the analysis of the existing land use/cover simulation and forecast models, a land use/cover scenario dynamics model by the integration of System Dynamics (SD) model, Back Propagation Neural Network (BPNN) and Cellular Automata (CA) model is developed with land use/cover scenario changes in northern china in the next 30 years and simulated in this paper. The model is to simulate the land use/cover scenario demands by using a SD model at first, and then allocating the land use scenario patterns at the local scale with the considerations of land use/cover suitability, inheritance ability and neighborhood effect by using BPNN-CA model to satisfy the balance between land use/cover scenario demands and supplies. It integrates the advantages of SD, BPNN and CA. Macro-driving factors and the micro-spatial pattern are also fully taken into account. The BPNN simplifies the identification of the factors’ weights used in CA model and improves the reliability of the simulation results. The simulation accuracy of the model developed in this paper was found to be about 74%. It suggests that the model has the ability to reflect the complexity of land use/cover system at different scales to some extent and it is a useful tool for assessing the potential impacts of land use system on ecosystem. The simulated results also indicate that the urban land, water area and forest will increase significantly, and farmland and unable land will decrease gradually. Obvious land use/cover changes will take place in the farming-pastoral zone and the southeast area of northern china.

关键词：土地利用中国北方细胞自动机系统动力学

Development of a high-throughput assay for the HIV-1 integrase disintegration reaction

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science china(life sciences) 2010年第2期53卷 241-247页

作者： HE HongQiu,LIU Bin,ZHANG XiaoYi,CHEN WeiZu & WANG CunXin college of life science and Bioengineering,beijing university of Technology,beijing 100124,china college of life science and Bioengineering Beijing University of Technology Beijing China

Both HIV-1 integrase (IN) and the central catalytic domain of IN (IN-CCD) catalyze the disintegration reaction in vitro.In this study,IN and IN-CCD proteins were expressed and purified,and a high-throughput format enzyme-linked immunosorbent assay (ELISA) was developed for the disintegration reaction.IN exhibited a marked preference for Mn2+ over Mg2+ as the divalent cation cofactor in disintegration.Baicalein,a known IN inhibitor,was found to be an IN-CCD inhibitor.The assay is sensitive and specific for the study of disintegration reaction as well as for the in vitro identification of antiviral drugs targeting IN,especially targeting IN-CCD.

关键词： HIV-1 integrase IN-CCD disintegration high-throughput assay

Soluble protein and acid phosphatase exuded by ectomycorrhizal fungi and seedlings in response to excessive Cu and Cd

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Journal of environmental sciences 2009年第12期21卷 1667-1672页

作者： ZHENG Weishuang, FEI Yingheng, HUANG Yi college of environmental sciences and Engineering, Peking university, beijing 100871, china. college of environmental sciences and Engineering Peking University Beijing 100871 China

Fungi and their symbionts can alleviate heavy metal stress by exuding soluble proteins and enzymes. This study examined the role of soluble protein and acid phosphatase （APase） exuded by Xerocomus chrysenteron, an ectomycorrhizal fungus, and the seedlings of its symbiont, Chinese pine （Pinus tabulaeformis）, under conditions of excessive Cu and Cd. The growth type showed that this poorly studied ectomycorrhizal fungus was capable of tolerating high concentrations of Cu, and may be useful in phytoremediation. X. chrysenteron grew well at 80 mg/L Cu, and the EC50 for Cd was 17.82 mg/L. X. chrysenteron also showed enhanced exudation of soluble protein in both isolated and inoculated cultivations under the influence of Cu and Cd. Soluble protein exudation, however, differed under Cu and Cd stress in isolates. In mediums containing Cu, soluble protein exudation increased with concentration, but in mediums containing Cd the content of soluble protein increased to a comparable level at all concentrations. This study demonstrated that soluble protein was related to heavy metal tolerance, although the different ions played different roles. While APase activity in exudates of fungi and seedlings decreased under Cu and Cd stress in comparison to the control, the APase activity in seedlings was maintained by inoculation. Thus, X. chrysenteron facilitated the ability of plant to maintain a normal nutrient uptake, and therefore to protect it from heavy metal toxicity.

关键词： soluble protein acid phosphatase exudate Cu, Cd Xerocomus chrysenteron