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Phylogeny of fireflies (Coleoptera: Lampyridae) inferred from mitochondrial 16S ribosomal DNA, with references to morphological and ethological traits

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Progress in Natural Science:Materials International 2006年第8期16卷 817-826页

作者： LI Xueyan, YANG Shuang, XIE Meng and LIANG Xingcai (Laboratory of Entomology, kunming Institute of zoology, Chinese Academy of Sciences, kunming 650223. china Key Laboratory of Cellular and Molecular Evolution, kunming Institute of zoology, Chinese Academy of Sciences, kunming 650223. china Graduate School of the Chinese Academy of Sciences, Beijing 10003., china) Laboratory of Entomology kunming Institute of zoology Chinese Academy of Sciences Kunming 650223 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Key Laboratory of Cellular and Molecular Evolution kunming Institute of zoology Chinese Academy of Sciences Kunming 650223 China

We sequenced partial mitochondrial 16S ribosomal DNA (16S rDNA) of 18 firefly species from Southwest of china. Combined with homologous sequences previously reported, phylogenetic trees including Japanese, Korean and Chinese species were reconstructed by neighbor-joining, maximum parsimony and Bayesian methods. All reconstructions agree on most nodes of the trees. Monophyly of Lampyridae is not supported because Rhagophthalmus ohbai in Rhagophthalmidae is included within it. Lamprigera, a genus placed unreliably in Lampyrinae, shows a close relationship to Amydetinae. Monophyly of Luciolinae is not supported because Pristolycus sagulatus (Lampyrinae) is included within it. In the Luciolinae, monophyly of Curtos and Hotaria is well established, respectively, but both morphological and molecular data continue to indicate that Luciola is not monophyletic and its subdivision is indeed necessary. Within the Lampyrinae, both Pyrocoelia and Diaphanes are not monophyletic, but monophyly of Pyrocoelia + Diaphanes is well supported. Phylogeny of Diaphanes is discussed for the first time. Generic placement of a newly discovered species (Diaphanes pectinealis Li et Liang) sharing some characters of Pyrocoelia and Diaphanes challenges the delimitation of these two similar genera. With references to the firefly mating systems, we suggest that more emphases should be placed on those sexually selected characters such as antennal structure in taxonomy of Lampyridae.

关键词： Lampyridae mitochondrial 16S rDNA morphological and ethological traits phylogeny.

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Pitfalls in the analysis of ancient human mtDNA

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Chinese Science Bulletin 2003年第8期48卷 826-830页

作者： YAO Yonggang1,3.& ZHANG Yaping1,2 1. Laboratory of Molecular Evolution and Genome Diversity, kunming Institute of zoology, Chinese Academy of Sciences, kunming 650223. china 2. Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, kunming 650091, china 3. Graduate School of the Chinese Academy of Sciences, Beijing 10003., china Correspondence should be addressed to Zhang Yaping (e-mail: zhang- yp1@***) Laboratory of Molecular Evolution and Genome Diversity Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming 650091 China

The retrieval of DNA from ancient human specimens is not always successful owing to DNA deteriora-tion and contamination although it is vital to provide new insights into the genetic structure of ancient people and to reconstruct the past history. Normally, only short DNA fragments can be retrieved from the ancient specimens. How to identify the authenticity of DNA obtained and to uncover the information it contained are difficult. We employed the ancient mtDNAs reported from Central Asia (including Xin-jiang, china) as an example to discern potentially extraneous DNA contamination based on the updated mtDNA phylog-eny derived from mtDNA control region, coding region, as well as complete sequence information. Our results demon-strated that many mtDNAs reported are more or less prob-lematic. Starting from a reliable mtDNA phylogeney and combining the available modern data into analysis, one can ascertain the authenticity of the ancient DNA, distinguish the potential errors in a data set, and efficiently decipher the meager information it harbored. The reappraisal of the mtDNAs with the age of more than 2000 years from Central Asia gave support to the suggestion of extensively (pre)historical gene admixture in this region.

关键词：古代人类遗传构造 DNA 脱氧核糖核酸

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Hemin-mediated Hemolysis in Erythrocytes:Effects of Ascorbic Acid and Glutathione

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 63-69页

作者： Shu-De LI~(1,2),Yan-Dan SU~3.Ming LI~1,and Cheng-Gang ZOU~(1*) ~1 School of Life Sciences,Yunnan University,kunming 650091,china ~2 Department of Biochemistry and~3.Department of Clinical Chemistry,kunming Medical College.kunming 65003.,china School of Life Sciences Yunnan University Kunming 650091 China Department of Biochemistry Kunming Medical College Kunming 650031 China Department of Clinical Chemistry Kunming Medical College Kunming 650031 China

In the present work,we investigated the effect of ascorbic acid and glutathione on hemolysisinduced by hemin in *** acid not only enhanced hemolysis,but also induced formationof thiobarbituric acid-reactive substances in the presence of *** has been shown that glutathioneinhibits hemin-induced hemolysis by mediating hemin *** depleted of glutathionebecame very sensitive to oxidative stress induced by hemin and ascorbic acid.H2O2was involved in hemin-mediated hemolysis in the presence of ascorbic ***,a combination of glutathione and ascorbicacid was more effective in inhibiting hemolysis induced by hemin than glutathione *** andintracellular ascorbic acid exhibited a similar effect on hemin-induced hemolysis or inhibition of hemin-induced hemolysis by *** current study indicates that ascorbic acid might function as anantioxidant or prooxidant in hemin-mediated hemolysis,depending on whether glutathione is available.

关键词： erythrocyte ascorbic acid hemin glutathione H2O2

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Application of the phylogenetic analysis in mitochondrial disease study

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Chinese Science Bulletin 2008年第18期53卷 2733-2738页

作者： WANG ChengYe1,2,3. KONG QingPeng1,2 & ZHANG YaPing1,2? 1 State Key Laboratory of Genetic Resources and Evolution, kunming Institute of zoology, Chinese Academy of Sciences, kunming 650223. china 2 Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, kunming 650091, china 3.Graduate University of Chinese Academy of Sciences, Beijing 100049, china State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming China Laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming China Graduate University of Chinese Academy of Sciences Beijing China

Mitochondrial disease currently received an increasing concern. However, the case-control design commonly adopted in this field is vulnerable to genetic background, population stratification and poor data quality. Although the phylogenetic analysis could help solve part of these problems, it has not received adequate attention. This paper is a review of this method as well as its application in mito- chondrial disease study.

关键词：线粒体疾病分析单元型种群分布

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On the periodic solutions of a type of differential-iterative equations

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Chinese Science Bulletin 1998年第3期43卷 204-206页

作者： GE Weigao 1, LIU Zhengrong 2 and YU Yuanhong 3.1. Department of Applied Mathematics, Beijing Institute of Technology, Beijing 100081, china 2. Department of Mathematics, Yunnan University, kunming 650091, china 3. Institute of Applied Mathematics, C Department of Applied Mathematics Beijing Institute of Technology Beijing China Department of Mathematics Yunnan University Kunming China Institute of Applied Mathematics Chinese Academy of Sciences Beijing China

Sufficient conditions for the existence of periodic solutions to a type of nonautonomous differential_iterative equations are given.

关键词： differential_iterative equation periodic solution fixed point.

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Asymmetric interaction will facilitate the evolution of cooperation

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Science china(Life Sciences) 2010年第8期53卷 1041-1046页

作者： WANG RuiWu1, HE JunZhou1,2, WANG YaQiang1,2, SHI Lei1,3.& LI YaoTang1,2 1 State Key Laboratory of Genetic Resources and Evolution, kunming Institute of zoology, Chinese Academy of Science, kunming 650223. china 2 School of Mathematics and Statistics, Yunnan University, kunming 650091, china 3.Statistics and Mathematics College, Yunnan University of Finance and Economics, kunming 650221, china State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Science Kunming China School of Mathematics and Statistics Yunnan University Kunming China Statistics and Mathematics College Yunnan University of Finance and Economics Kunming China

Explaining the evolution of cooperation remains one of the greatest problems for both biology and social science. The classical theories of cooperation suggest that cooperation equilibrium or evolutionary stable strategy between partners can be maintained through genetic similarity or reciprocity relatedness. These classical theories are based on an assumption that partners interact symmetrically with equal payoffs in a game of cooperation interaction. However, the payoff between partners is usually not equal and therefore they often interact asymmetrically in real cooperative systems. With the Hawk–Dove model, we find that the probability of cooperation between cooperative partners will depend closely on the payoff ratio. The higher the payoff ratio between recipients and cooperative actors, the greater will be the probability of cooperation interaction between involved partners. The greatest probability of conflict between cooperative partners will occur when the payoff between partners is equal. The results show that this asymmetric relationship is one of the key dynamics of the evolution of cooperation, and that pure cooperation strategy (i.e., Nash equilibrium) does not exist in asymmetrical cooperation systems, which well explains the direct conflict observed in almost all of the well documented cooperation systems. The model developed here shows that the cost-to-benefit ratio of cooperation is also negatively correlated with the probability of cooperation interaction. A smaller cost-to-benefit ratio of cooperation might be created by the limited dispersal ability or exit cost of the partners involved, and it will make the punishment of the non-cooperative individuals by the recipient more credible, and therefore make it more possible to maintain stable cooperation interaction.

关键词： Hawk–Dove game evolutionarily stable strategy asymmetric cooperation mixed strategy Nash equilibrium

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基于凸包理论的含风电电力系统负荷恢复方案优化

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电力系统自动化 2024年第5期48卷 77-87页

作者：伊昆明孙磊丁江丁明合肥工业大学电气与自动化工程学院安徽省合肥市230009 新能源利用与节能安徽省重点实验室(合肥工业大学) 安徽省合肥市230009 国网南昌供电公司江西省南昌市330000

大停电事故后,合理的负荷恢复策略对加快电力系统恢复、减少停电损失具有重要意义。针对标准交流潮流方程的非凸性导致负荷恢复模型求解效率低的问题,提出了基于凸包理论的含风电电力系统负荷恢复方案优化方法。首先,简要介绍了凸包理... 详细信息

大停电事故后,合理的负荷恢复策略对加快电力系统恢复、减少停电损失具有重要意义。针对标准交流潮流方程的非凸性导致负荷恢复模型求解效率低的问题,提出了基于凸包理论的含风电电力系统负荷恢复方案优化方法。首先,简要介绍了凸包理论以及非线性函数的凸包构建方法。其次,针对风电出力的不确定性,采用条件风险价值理论处理风电波动带来的风险,进而建立了直角坐标系下基于凸包理论的电力系统负荷恢复二阶锥优化模型,其中,交流潮流方程中的非线性项采用凸包表达。针对无法精确确定凸包参数的问题,提出了基于迭代更新的参数优化方法,减少因凸包引入的松弛误差。所提出的模型为混合整数二阶锥规划模型,可采用商业求解器高效求解。最后,采用IEEE 3.节点系统和改进的广东电力系统验证了所提出模型的有效性。

关键词：电力系统风电潮流负荷恢复凸包理论直角坐标混合整数二阶锥规划

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Enzymatic cyclization of linear peptide to plant cyclopeptide heterophyllin B

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Science china Chemistry 2006年第1期49卷 63-66页

作者： JIA Aiqun1, LI Xiang2, TAN Ninghua1, LIU Xiaozhu3. SHEN Yuemao1 & ZHOU Jun1 1. State Key Laboratory of Phytochemistry and Plant Resources in West china, kunming Institute of Botany, Graduate Univer-sity of Chinese Academy of Sciences, Chinese Academy of Sciences, kunming 650204, china 2. Chinese Peptide Co., Ltd, Hangzhou 3.0018, china 3. Southwest Forestry College, kunming 650204, china State Key Laboratory of Phytochemistry and Plant Resources in West china Kunming Institute of Botany Graduate University of Chinese Academy of Sciences Chinese Academy of Sciences Kunming China Chinese Peptide Co. Ltd Hangzhou China Southwest Forestry College Kunming China

The crude enzyme (PH-1) isolated from Pseudostellaria heterophylla by ourgroup has catalyzed enzymatic cyclization of linear peptideNH_2-Gly^1-Gly^2-Leu-Pro-Pro-Pro-lle-Phe-COOH (4) into cyclopeptide heterophyllin B (HB) from plantfor the first time. To ensure this reaction, some analytical methods including TLC, HPLC, MS, NMR,and ^(13.C labeling were used to prove that the reaction of substrate 4 sharing residue ofNH-Phe-Gly-CO was successful.

关键词： Pseudostellaria heterophylla, cyclopeptides, HB, enzymatic cyclization, isotope labeled.

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The topological configuration and conformational analysis of mRNA in translation

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Chinese Science Bulletin 2002年第5期47卷 384-389页

作者： LIU Shuqun & LIU Ciquankunming Institute of zoology, Chinese Academy of Sciences, kunming 650223. china Modern Biological Center, Yunnan University, kunming 650091, china kunming Institute of zoology Chinese Academy of Sciences Kunming China Modern Biological Center Yunnan University Kunming China

The theoretical model construction of mRNA hairpin structure and single-stranded structure as well as the simulation studies on RNA structure determined by the X-ray crystal diffraction and nuclear magnetic resonance revealed that in translation, after mRNA being unfolded into single-stranded structure, its topological configuration was closely correlative with the original hairpin structure. The conformational features of single-stranded mRNA appeared as helical regions alternating with curly regions to different extents, which might exert the influence on the folding of nascent polypeptide by various regulating effects including different translational rates.

关键词： hairpin structure of mRNA topological configuration of single-stranded mRNA.

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Morphological characteristics of leaf epidermis and size variation of leaf,flower and fruit in different ploidy levels in Buddleja macrostachya (Buddlejaceae)

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Journal of Systematics and Evolution 2009年第3期47卷 231-236页

作者： 1,2Gao CHEN 1Wei-Bang SUN 3.ang SUN 1(kunming Botanical Garden, kunming Institute of Botany, Chinese Academy of Sciences, kunming 650204, {3.) 2(Graduate School of Chinese Academy of Sciences, Beijing 100049, {3.) 3.Key Laboratory of Biodiversity and Biogeography, kunming Institute of Botany, Chinese Academy of Sciences, kunming 650204, {3.) (kunming Botanical Garden Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Graduate School of Chinese Academy of Sciences Beijing 100049 China) (Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China)

Buddleja macrostachya (Buddlejaceae) is a widespread shrub native to the Sino-Himalayan mountains and beyond. It has been found to occur at two ploidy levels, hexaploid, 2n=6x=114 and dodecaploid, 2n=12x=228. To determine if morphological characters might be used as indicators of ploidy levels, we measured floral and fruit length, relative and absolute leaf size, trichome density on both leaf surfaces, and stomatal density and length in different populations of B. macrostachya. In general, flower and fruit length, absolute leaf size, and stomatal length increased with an increase at ploidy level (P0.05) in different populations. Other characters studied such as trichome type, cuticular membrane and ornamentation of stomata, cell and stomatal shape, and anticlinal wall pattern were quite constant in this species. Thus it appears that flower and fruit length, absolute leaf size, and stomatal frequency and length can be used to distinguish hexaploid from dodecaploid cytotypes either in the fleld or in herbarium specimens.

关键词： Buddleja macrostachya dodecaploid hexaploid leaf epidermis ploidy level stomata.

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