检索结果-南通市图书馆

Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2007年第1期6卷 25-32页

作者： WANG Han-yan1, WEI Yu-ming1, 2, ZE Hong-yan1, 2 and ZHENG You-liang1, 21 Triticeae Research Institute, Sichuan Agricultural University, ya’an 625014, P.R.china2 key laboratory of crop genetic resources and improvement in southwest china, ministry of education/Sichuan Agricultural University,ya’an 625014, P.R.china Triticeae Research Institute Sichuan Agricultural University Ya'an 625014 P.R. China key laboratory of crop genetic resources and improvement in southwest china Ministry of Education/Sichuan Agricultural University Ya'an 625014 P.R. China

Three coding sequences of gliadins genes, designed as Gli2_Du1, Gli2_Du2 and Gli2_Du3, were isolated from thegenomic DNA of Triticum durum accessions CItr5083. Gli2_Du1 and Gli2_Du2 contain 945 and 864 bp, encoding themature proteins with 314 and 287 amino acid residues, respectively. Gli2_Du3 is recognized as a pseudogene due to thestop codon occurring in the coding region. The pseudogenes, commonly occurring in gliadins family, are attributed to thesingle base change C → T. The amino acid sequences deduced from these gene sequences were characterized with thetypical structure of α-gliadin proteins, including the toxic sequences (PSQQQP). The peptide fraction PF(Y)PP(Q) isthought to be an extra unit of repetitive domain, slightly diverging from the previous report. Six cysteine residues wereobserved within two unique domains. Phylogenetic analysis showed Gli2_Du2 and Gli2_Du3 were closely related to thegenes on chromosome 6A, whereas Gli2_Du1 seems to be more homologous with the genes on chromosome 6B.

关键词： durum wheat α-gliadin gene clone sequence analysis

Evaluation and Quantitative Inheritance of Several Drought-Relative Traits in Maize

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2008年第3期7卷 280-290页

作者： FU Feng-ling FENG Zhi-lei GAO Shi-bing ZHOU Shu-feng LI Wan-chen key laboratory of crop genetic resources and improvement,ministry of education/Maize Research Institute.Sichuan Agricultural University,ya’an 625014,P.R.china key laboratory of crop genetic resources and improvement Ministry of Education/Maize Research Institute Sichuan Agricultural University Ya'an 625014 P.R. China

The authors evaluated 57 parental inbred lines of maize hybrids disseminated in southwest china for drought tolerance under drought-stressed and well-watered *** regression analyses between drought tolerant coefficients of the grain yield per plant and 15 morphological and physiological traits measured from a subset of 12 selected lines, identified traits 1 and 5,which were important for drought tolerance,at the seedling and reproductive stages respectively. Gene effects,combining abilities,and heritabilities of these traits were estimated using generation mean and diallel cross *** effect was more important than additive effect for the plant height,anthesis-silking interval(ASI), root weight,and the grain yield per plant,whereas,they were about equal for the leaf emergence *** variances of special combining ability(SCA)were about double that of the general combining ability(GCA)for plant height,ASI and grain yield per plant,although they were about equal for leaf emergence rate and root *** sense beritabilities of the five traits for the reproductive stage were not high(12.8-29.6%),although broad sense heritabilities for plant height, ASI,and grain yield were as high as 70-85%.A segregating population consisting of 183 Fplants from the cross N87-1 (drought tolerant)×9526(susceptible),was genotyped at 103 SSR loci and the Ffamilies were evaluated under two water *** quantitative trait loci(QTLs)(two for plant height,five for ASI,four for root biomass,and one for grain yield)were identified,most of which had overdominant gene *** chromosomal regions,such as those linked to markers umc1051(bin 4.08),umc2881(bin 4.03),and phi034(bin 7.02),had overlapping QTLs.

关键词： maize drought tolerance evaluation quantitative inheritance QTL

Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10.1 Ecoding Gene from Xinjiang Wheat (Triticum petropavlovskyi Udacz. et Migusch)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2006年第2期5卷 81-89页

作者： JIANG Qian-tao1, 2*, WEI Yu-ming1, 2*, WANG Ji-rui1, 2, yaN Ze-hong1, 2 and ZHENG You-liang1, 2 1 Triticeae Research Institute, Sichuan Agricultural University, ya’an 625014, P.R.china 2 key laboratory of crop genetic resources and improvement, ministry of education/Sichuan Agricultural University, ya’an 625014, P.R.china Triticeae Research Institute Sichuan Agricultural University Ya'an 625014 P.R. China key laboratory of crop genetic resources and improvement Ministry of Education/Sichuan Agricultural University Ya'an 625014 P.R. China

A novel HMW glutenin subunit gene 1Dy10.1 was isolated and characterized from Xinjiang wheat (Triticum petropavlovskyi. Udacz. et Migusch) accession Daomai 2. The complete open reading frame (ORF) of 1Dy10.1 was 1 965 bp, encoding 655 amino acids. The numbers and distribution of cysteines in 1Dy10.1 were similar to those of 1Dy10 and other y-type subunits. In the N-terminal of 1Dy10.1, an amino acid was changed from L (leucine) to P (proline) at position 55. The repetitive domain of 1Dy10.1 differed from those of known HMW subunits by substitutions, insertions or/and deletions involving single or more amino acid residues. In the repetitive domain of subunit 1Dy10.1, the deletion of tripeptide GQQ in the consensus unit PGQGQQ resulted in the appearance of the motif PGQ that have not been observed in other known y-type HMW subunits. In comparison with the subunit 1Dy12, a deletion of dipeptide GQ, which occurred in subunit 1Dy10, was also observed in subunit 1Dy10.1. The cloned 1Dy10.1 gene had been successfully expressed in Escherichia coli, and the expressed protein had the identical mobility with the endogenous subunit 1Dy10.1 from seed.

关键词： Triticum petropavlovskyi sequence analysis HMW-GS gene bacterial expression

Characterization of WAP2 gene in Aegilops tauschii and comparison with homoeologous loci in wheat

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Systematics and Evolution 2009年第6期47卷 543-551页

作者： ~(1,2)Shun-Zong NING ~(1,2)Qi-Jiao CHEN ~(1,2)Zhong-Wei YUAN ~(1,2)Lian-Quan ZHANG ~(1,2)Ze-Hong yaN ~(1,2)You-Liang ZHENG ~(1,2,3)Deng-Cai LIU~* 1(Triticeae Research Institute,Sichuan Agricultural University,Wenjiang of Chengdu City,Sichuan 611130,china) 2(key laboratory of crop genetic resources and improvement,ministry of education,Sichuan Agricultural University,ya’an,Sichuan 625014,china) 3(Northwest Plateau Institute of Biology,Chinese Academy of Science,Qinghai 810001,china) (Triticeae Research Institute Sichuan Agricultural University Wenjiang of Chengdu City Sichuan 611130 China) (key laboratory of crop genetic resources and improvement Ministry of Education Sichuan Agricultural University Ya'an Sichuan 625014 China) (Northwest Plateau Institute of Biology Chinese Academy of Science Qinghai 810001 China)

The Q/q gene,also known as WAP2,is an important gene for wheat domestication and is a member of the AP2(APETALA2) class of transcription *** the present study,we first isolated the W~tAP2 allele(where the superscript"t"refers to the speciese source,in this case"tauschii") on chromosome 5D from Aegilops tauschii Coss.,the D-genome donor species of common *** found that W~tAP2 and the AP2 gene from Arabidopsis share a central core of the AP2 polypeptide,a highly basic 10-amino acid domain,and an AASSGF box,although there are many differences in the 37-amino acid serine-rich acidic domain and the remaining *** addition, W~tAP2 was highly homologous to the homoeologous loci on 5A and 5B of wheat at both the nucleotide and amino acid ***,there were some variations that are probably related to gene *** the first AP2 domain, the amino acids VYL on the 5D and 5A loci were replaced with LLR on *** the 37-amino acid serine-rich acidic domain,W~tAP2 on 5D had an extra amino acid *** was also a variation at the 329 amino acid position, which is thought to be related to the appearance of free-threshing *** this position,the amino acid is isoleucine on 5A for the Q allele and valine for the q allele,whereas the amino acid is leucine on 5D and ***, a Stowaway miniature terminal inverted repeat element(MITE) insertion was present in the ninth intron of WAP2 on 5B of all common wheats and partial tetraploid Triticum turgidum *** results provide new clues for studies into the evolutionary biology of WAP2 and the origin of common wheat.

关键词： Aegilops tauschii AP2 gene miniature terminal inverted repeat element(MITE) Q gene wheat

Patterns of DNA cytosine methylation between haploids and corresponding diploids in rice

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2006年第14期51卷 1721-1728页

作者： ZHANG Hongyu1,2*, PENG Hai1,2*, LI Yun1,2, XU Peizhou1,2, WANG Xudong1,2 & WU Xianjun1,21. Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, Sichuan 2. key laboratory of southwest crop genetic resources and Improve-ment, ministry of education, Sichuan Agricultural University, ya’an 625014, china Rice Research Institute of Sichuan Agricultural University Wenjiang China key laboratory of southwest crop genetic resources and improvement Ministry of Education Sichuan Agricultural University Ya’an China

Eighteen pairs of diploid-haploid twin-seedlings were identified and screened out fromspecial rice SARII-628 population. Five pairs of themwere selected and randomly designated as A, B, C, Dand E. Simple sequence repeats (SSR) analysisshowed that there was no difference among 310 siteswhich indicated that there was no base mutation onDNA primary structure. DNA methylation plays animportant role in gene expression regulation duringgrowth and development stages in eukaryotes. Amodified AFLP technique (methylation-sensitiveAFLP, MSAP) was employed to detect the DNA me-thylation patterns in the 5′-CCGG sites of the fivepairs of twin-seedlings. Although no methylationmutation was detected among the five diploids,forty-three methylation mutation sites were foundfrom the corresponding haploids. The MSAP ratios,which were the ratios of MSAP sites to the total am-plified sites, in five haploids were 18.79%, 19.35%,18.49%, 18.45% and 18.75%, respectively. And cor-responding full methylation levels (5′-CmCGG indouble strands) of those haploids were 10.58%,11.3%, 10.11%, 10.09% and 10.34%, *** MSAP and full methylation levels in the fivehaploids were higher than that of their correspondingdiploids, which suggested that hypermethylation oc-curred in some 5′-CCGG sites. Five types of MASPpatterns among the five pairs of twin-seedlings weredetected as follows: (1) no changes, methylation lev-els were the same in both haploids and diploids; (2) demethylation, diploid was methylated but no me-thylation in the same site in haploid; (3) hypermethy-lation, the methylation level in haploid was higher than those in diploid; (4) hypomethylation, methyla-tion in haploid was lower than those in diploid; (5) undecided pattern, change trend of methylation lev-els in haploids was not decided. The bands of 18 sites were reclaimed, then sequenced and searched on website to determine the sites of those sequences on rice chromosomes. The result showed that the methylation mutation in

关键词： rice twin-seedling diploid haploid DNA methylation MSAP.

Characteristics of Rice Plant with Heavy Panicle

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2006年第12期5卷 911-918页

作者： MA Jun1, 2, MA Wen-bo3, MING Dong-feng4, yaNG Shi-ming1, 2 and ZHU Qing-sen5 1 Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, P.R.china 2 key laboratory of southwest crop genetic resources and improvement, ministry of education/Sichuan Agricultural University, yaan 625014, P.R.china 3 Zaozhuang Agriculture Bureau, Shandong Province, Zaozhuang 277160, P.R.china 4 Zaozhuang Teacher College, Zaozhuang 277160, P.R.china 5 College of Agronomy, yangzhou University, yangzhou 225009, P.R.china Rice Research Institute Sichuan Agricultural University Wenjiang 611130 P.R.China key laboratory of southwest crop genetic resources and improvement Ministry of Education/Sichuan Agricultural University Yaan 625014 P.R.China Zaozhuang Agriculture Bureau Shandong Province Zaozhuang 277160 P.R.China Zaozhuang Teacher College Zaozhuang 277160 P.R.China College of Agronomy Yangzhou University Yangzhou 225009 P.R.China

Using 15 indica rice varieties with different panicle weight, some ideal plant type characteristics in heavy panicle type of hybrid rice (HPT) and their relation to yield and its components were studied. Results showed that the leaf area index (LAI) of the HPT varieties was lower than that of medium panicle type (MPT) and light panicle type (LPT) varieties, but its decreasing rate of LAI and efficient LAI after heading was slower and had much higher percentage of efficient LAI, specific leaf weight, and ratio of grain to leaf area (cm2) in comparison with the MPT and the LPT varieties. The length, width, thickness, and area of top three leaves of the HPT varieties were significantly larger than those of the MPT and the LPT varieties, and these components of top three leaves were significantly and positively correlated with the number of spikelets and filled grains, grain weight per panicle, and grain yield. The flag leaf in HPT varieties was erect with sorrow leaf angle, and their leaf angle of 2nd and 3rd leaf from top increased in sequence. The plant height of the HPT varieties was higher than that of the MPT and the LPT varieties, and their leaf site of top three leaves also increased in sequence. Therefore, HPT varieties as an ideal plant type could increase the utilization efficiency of sunlight energy. The ideal plant type characteristics and their adjuncts for the HPT varieties are proposed in this article.

关键词： heavy panicle type rice plant type characteristics

Analysis of Chloroplast Ultrastructure,Photosystem Ⅱ Light Harvesting Complexes and Chlorophyll Synthesis in a Chlorophyll-Less Rice Mutant W2555

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Rice Science 2006年第4期 265-270页

作者： XU Pei-zhou 1,3, LI Yun 1, YUAN Shu 2, ZHANG Hong-yu 1, WANG Xu-dong 1, LIN Hong-hui 2, WU Xian-jun 1 (1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, china 2key laboratory of Bio-resources and Eco-environment, ministry of education College of Life Sciences, Sichuan University, Chengdu 610064, china 3key laboratory of southwest crop genetic resources and improvement, ministry of education Sichuan Agricultural University, ya’an 625014, china)

A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ (LHC Ⅱ) was conducted between a new rice mutant (W2555) and its wild type (WT). The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type. There was no significant change in the composition of LHC Ⅱ polypeptide in W2555, while a decline had been noted in LHC Ⅱ content. Northern blot analysis with a specific cab gene probe showed no appreciable difference in the LHC Ⅱ mRNA level between the W2555 and its wild type. The precursors of chlorophyll synthesis, δ-aminolevulinic acid (ALA) and porphobilinogen (PBG) were over accumulated in W2555, but the other precursors were all decreased. These results indicated that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen Ⅲ (Urogen Ⅲ).

关键词： chloroplast ultrastructure photosystem Ⅱ chlorophyll synthesis precursors chlorophyll-less mutant rice

Transformation of Maize with Trehalose Synthase Gene Cloned from Saccharomyces cerevisiae

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Transformation of Maize with Trehalose Synthase Gene Cloned ...

Purification and Analysis of Abscisic Acid-Specifically-Inducible Proteins from Rice Callus

2009整合植物生物学研讨会

作者： Yu Mu,Dan Tao,yan Zhang,Xiao-Juan Shen,Cui Hu,Feng-Ling Fu and Wan-Chen Li~* Maize Research Institute/education ministry key laboratory of crop genetic resources and improvement,Sichuan Agricultural University,ya’an 625014,china.

Abiotic stresses are the most important limiting factor in maize production,especially drought that threaten to the grain yield of *** most effective and economical way to stabilize and improve maize production under drought conditions is to improve the varieties for drought ***,breeding for this trait is particularly challenging because of

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rice Science 2007年第2期14卷 111-117页

作者： JIANG Hua 1, 2, XU Zheng-jun 2, 3, GAO Xiao-ling 2 (1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, china 2Department of Food and Bioengineering, Shandong Institute of Light Industry, Ji’nan 250353, china 3 key laboratory of crop genetic resources and improvement, ministry of education, Sichuan Agricultural University, ya’an 625014, china) Rice Research Institute Sichuan Agricultural University Wenjiang 611130 China Department of Food and Bioengineering Shandong Institute of Light Industry Ji'nan 250353 China key laboratory of crop genetic resources and improvement Ministry of Education Sichuan Agricultural University Ya'an 625014 China

Two ABA-specifically-inducible proteins from rice callus were isolated and purified by precipitation with 65-100% saturated (NH4)2SO4, followed by the DEAE-sepharose, TSK-gel, and two-dimension electrophoresis. Iso-electric points (pI) of the proteins with the same molecular mass (24.5 kD) were 6.1 and 6.9, respectively. The Western blot analysis indicated that the proteins expressed in different tissues were obviously different. The A1 (pI 6.1) protein was only detected in calli treated with ABA and seed embryos (SE). However, the A2 (pI 6.9) protein was found not only in the calli treated with ABA and SE, but also in the white dry callus. Thus it suggested that the two proteins might play some important roles in the processes of seed embryo (or somatic embryo) formation.

关键词： rice callus abscisic acid specifically-inducible protein purification expression