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Progress in Natural Science:Materials International 2009年第11期19卷 1581-1586页

作者： Fei Wu~a,Yinghua Huang~a,Ying Ma~b,Shengqiang Hu~c,Jinping Hao~c,Ning Li~a a State key laboratory for Agrobiotechnology,china agricultural university,beijing 100193,china b college of Animal Science and veterinary Science,Inner Mongolia agricultural university,Hohhot 010018,china c Gold Star Duck Production Ltd.,beijing 100076,china State key laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China college of Animal Science and veterinary Science Inner Mongolia Agricultural University Hohhot 010018 China Gold Star Duck Production Ltd. Beijing 100076 China

The genetic diversity of two natural populations(M,N) of beijing duck(Anas platyrhynchos) and 11 artificially selected lines of beijing duck(A,B,E-L,O) from china Gold Star Duck Production Ltd.,along with two Cherry Valley duck lines(C and D) from the British Cherry Valley Livestock Division,was evaluated using 18 microsatellite markers covering 16 linkage groups.A phylogenetic tree of the 15 populations of duck,formed of four main branches,was constructed from Nei’s DAgenetic *** mean genetic differentiation index(FST) in all loci,Nei’s standard genetic distance(Ds),and the genetic distance DAbetween the beijing duck and the Cherry Valley duck were 0.075,0.143 and 0.142,*** results demonstrated a high degree of genetic similarity between the two breeds and supported the hypothesis that the Cherry Valley duck was derived from the beijing *** FSTmatrix of seven clusters of beijing duck suggested that the efficiency of selection was not significant to some extent and should be supplemented by marker -assisted selection.

关键词： Duck Microsatellite marker Genetic diversity Genetic distance

Molecular characterization of the membrane glycoprotein （M） gene of avian paramyxovirus serotype 2

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Molecular characterization of the membrane glycoprotein （M...

Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco

The 15th World veterinary Poultry Congress

作者： Guozhong Zhang Jixun Zhao Ming Wang Department of preventive veterinary medicine college of veterinary medicine china agricultural university beijing 100094 P.R.china.

＜正＞The Yucaipa virus was first isolated in 1956 from the tracheal exudates of chickens with laryngotra- *** virus was named as PMV-2/Chicken/California/Yucaipa/56 in 1972,and belongs to the genus Avulavirus within ...

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 553-558页

作者： Yun-Jun LIU, Yuan YUAN, Jun ZHENG, Ya-Zhong TAO, Zhi-Gang DONG,Jian-Hua WANG, and Guo-Ying WANGState key laboratory for Agrobiotechnology and National Center for Maize Improvement, china agricultural university, beijing 100094, china State key laboratory for Agrobiotechnology and National Center for Maize Improvement China Agricultural UniversityBeijing 100094 China

The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.

关键词： Cry1Ac signal peptide GFP transgenic tobacco

Altered expression of nuclear matrix proteins in etoposide induced apoptosis in HL-60 cells

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Cell Research 2001年第2期11卷 125-134页

作者： JIN MEI LIN, PING ZHANG, MING XIAO DING, JING PING YUN, PEI FANG CHEN, YEUK HON CHEN, YIN QING CHEW college of Animal Science and veterinary medicine, Huazhong agricultural university, Wuhan 430070, china college of Life Sciences, beijing university, Beij college of Animal Science and veterinary medicine Huazhong Agricultural University Wuhan China Chinese university of Hong Kong Hong Kong China college of Life Sciences Beijing University Beijing China

The events of cell death and the expression of nuclear matrix protein (NMP) have been investigated in a promyelocytic leukemic cell line HL-60 induced with etoposide. By means of TUNEL assay, the nuclei displayed a characteristic morphology change, and the amount of apoptotic cells increased early and reached maximun about 39% after treatment with etoposide for 2 h. Nucleosomal DNA fragmentation was observed after treatment for 4 h. The morphological change of HL-60 cells, thus, occurred earlier than the appearance of DNA ladder. Total nuclear matrix proteins were analyzed by 2-dimensional gel electrophoresis. Differential expression of 59 nuclear matrix proteins was found in 4 h etoposide treated cells. Western blotting was then performed on three nuclear matrix acssociated proteins, PML, HSC70 and NuMA. The expression of the suppressor PML protein and heat shock protein HSC70 were significantly upregulated after etoposide treatment, while NuMA, a nuclear mitotic apparatus protein, was down regulated. These results demonstrate that significant biochemical alterations in nuclear matrix proteins take place during the apoptotic process.

关键词： Nuclear matrix protein etoposide apoptosis HL-60 cell.

Characterization of codon usage bias in the dUTPase gene of duck enteritis virus

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Progress in Natural Science:Materials International 2008年第9期18卷 1069-1076页

作者： Lichan Zhao a, Anchun Cheng a,b,, Mingshu Wang a,b,, Guiping Yuan c, Mingsheng Caia a Avian Disease Research Center, college of veterinary medicine of Sichuan agricultural university, Xinkang Road 46, Ya’an, Sichuan 625014, china b key laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014, china c Analytical & Testing Center, Sichuan university, Chengdu 610064, china Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Xinkang Road 46 Ya’an Sichuan 625014 China key laboratory of Animal Disease and Human Health of Sichuan Province Ya’an 625014 China Analytical & Testing Center Sichuan University Chengdu 610064 China

A comparative analysis of the codon usage bias in the newly discovered dUTPase gene (Assigned Accession No.:DQ486149) of the duck enteritis virus (DEV) and the dUTPase gene of 32 reference herpesviruses was *** results indicated that the DEV dUT-Pase gene encodes a protein of 477 amino acids, which includes five conserved motifs with a 3-1-2-4-5 *** codon adap-tation index (CAI), efiective number of codons (ENC), and GC3S values indicated synonymous codon usage bias in the dUTPase gene of herpesviruses, and this synonymous bias was correlated with host *** codon usage patterns of the DEV dUTPase gene were phylogenetically conserved and similar to that of the dUTPase genes of the avian *** codon usage in each micro-organism was difierent, there were no strain-specific difierences among ***-one codons in the predicted polypeptide, with a strong bias towards A and T at the third codon position, were *** of the codon usage in the dUTPase gene of difierent organisms revealed that there were 19 codons showing distinct codon usage difierences between the DEV and Escherichia coli dUTPase genes;16 between the DEV and yeast dUTPase genes;and 15 between the DEV and human dUTPase *** of variance (ANOVA) showed significant difierences between the DEV and yeast dUTPase genes (r = 0.536, P < 0.01).The extent of codon usage bias in the DEV dUTPase gene was highly correlated with the gene expression level, therefore the results may provide useful information for gene classification and functional studies.

关键词： DEV dUTPase Codon usage bias EMBOSS

Seasonal influence on the development of parthenotes and cloned embryos in pigs

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Progress in Natural Science:Materials International 2007年第10期17卷 1172-1176页

作者： Zhang Kun1, Zhang Yunhai3,4, Pan Dengke5, Ma Yufang1, Wei Hengxi1, Zhang Jian6, Li Xuyang7, Wang Shaohua1, Li Yan2, Dai Yunping1,2 and Li Ning1(1. State key laboratory for Agrobiotechnology, college of Biological Science, china agricultural university, beijing 100094, china 2. JiPulin Biotech Ltd., beijing 100094, china 3. Danish Institute of agricultural Sciences, 8830 Tjele, Denmark 4. college of Animal Science and Technology, Anhui agricultural university, Hefei 230036, china 5. Institute of Animal Sciences, Chinese Academy of agricultural Sciences, beijing 100094, china 6. Animal Clinical Hospital, china agricultural university, beijing 100094, china 7. Hebei Minghui Group of Pigs Breeding, Sanhe 065200, china) State key laboratory for Agrobiotechnology College of Biological Science China Agricultural University Beijing 100094 China Danish Institute of agricultural Sciences 8830 Tjele Denmark college of Animal Science and Technology Anhui Agricultural University Hefei 230036 China Institute of Animal Sciences Chinese Academy of Agricultural Sciences Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China Hebei Minghui Group of Pigs Breeding Sanhe 065200 China JiPulin Biotech Ltd. Beijing 100094 China

Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (P<0.05). However, no significant difference was found in the rate of oocytes progressing to MⅡ in four seasons (P<0.05). In addition, the rate of blastocyst formation of parthenogenetic embryos in summer was obviously declined compared to the other seasons (P<0.05), and an increased blastocyst rate of cloned porcine embryos was found in spring compared to autumn and winter (P<0.05). The results suggest that there should be a seasonal influence on the developmental competence of porcine embryos.

关键词： pig cloned parthenogenetic season

Generation of fad2 transgenic mice that produce omega-6 fatty acids

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Science china(Life Sciences) 2009年第11期52卷 1048-1054页

作者： CHEN Qing1, LIU Qing2, WU ZhiFang1, WANG ZongYi3 & GOU KeMian1 1 State key laboratory for Agrobiotechnology, china agricultural university, beijing 100193, china 2 Blackmountain laboratory, CSIRO Plant Industry, Canberra, Australia 3 college of Animal Science and Technology, china agricultural university, beijing 100193, china State key laboratory for Agrobiotechnology China Agricultural University Beijing China Blackmountain laboratory CSIRO Plant Industry Canberra Australia college of Animal Science and Technology China Agricultural University Beijing China

Fatty acid desaturase-2 (FAD2) introduces a double bond in position 12 in oleic acid (18:1) to form linoleic acid (18:2 n-6) in higher plants and microbes. A new transgenic expression cassette, containing CMV promoter/fad2 cDNA/SV40 polyA, was constructedto produce transgenic mice. Among 63 healthy offspring, 10 founders (15.9%) integrated the cotton fad2 transgene into their genomes, as demonstrated by PCR and Southern blotting analysis. All founder mice were fertile and heterozygous fad2 female and nontransgenic littermates were used for fatty acid analysis using gas chromatography. One fad2 transgenic line showed substantial differences in the fatty acid profiles and the level of linoleic acid was increased 19% (P20:4 n-6) in muscles and liver, compared to their nontransgenic littermates. The results indicate that the plant fad2 gene can be functionally expressed in transgenic mice and may playan active role in conversion of oleic acid into linoleic acid.

关键词： fatty acid desaturase FAD2 transgenic mouse oleic acid linoleic acid arachidonic acid

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Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle

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Science china(Life Sciences) 2009年第3期52卷 296-306页

作者： LUO WeiZhen,WANG YaChun & ZHANG Yuan Department of Animal Genetics & Breeding,college of Animal Science and Technology,china agricultural university,beijing 100094,china Department of Animal Genetics & Breeding College of Animal Science and Technology China Agricultural University Beijing China

Advantages of breeding schemes using genetic marker information and/or multiple ovulation and embryo transfer(MOET) technology over the traditional approach were extensively evaluated through *** yield was the trait of interest and QTL was the genetic marker *** dairy cattle breeding scenarios were considered,i.e.,traditional progeny testing breeding scheme(denoted as STANPT),GASPT scheme including a pre-selection of young bulls entering progeny testing based on their own QTL information,MOETPT scheme using MOET technology to generate young bulls and a selection of young bulls limited within the full-sib family,GAMOPT scheme adopting both QTL pre-selection and MOET technology,COMBPT scheme using a mixed linear model which considered QTL genotype instead of the BLUP model in GAMOPT,and three non-progeny testing schemes,*** MOET,GAMO and COMB schemes,corresponding to MOETPT,GAMOPT and COMBPT with progeny testing being part of the *** were selected based on their breeding value which was estimated under an animal model *** selection over 17 years was performed in the simulations and 30 replicates were designed for each *** influences of using QTL information and MOET technology on favorable QTL allele frequency,true breeding values,polygenetic breeding values and the accumulated genetic superiority were extensively evaluated,for five different populations including active sires,lactating cows,bull dams,bull sires,and young *** results showed that the combined schemes significantly outperformed other approaches wherein accumulated true breeding value *** difference between schemes exclusively using QTL information or MOET technology was not *** STANPT scheme was the least efficient among the 8 *** schemes using MOET technology had a higher polygenetic response than others in the 17th *** increases of frequency of the favorable QTL allele varied more greatly across t

关键词： dairy cattle marker-assisted selection two-stage selection MOET QTL

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Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)

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Progress in Natural Science:Materials International 2007年第10期17卷 1177-1184页

作者： Li Zhenxing, Ni Zhongfu, Peng Huiru, Liu Zhiyong, Nie Xiuling, Xu Shengbao, Liu Gang and Sun Qixin(key laboratory of Crop Heterosis and Utilization and State key laboratory for Agrobiotechnology, key laboratory of Crop Genomics and Genetic Improvement, beijing key laboratory of Crop Genetic Improvement, Department of Plant Genetics & Breeding, china agricultural university, beijing 100094, china) key laboratory of Crop Heterosis and Utilization Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China key laboratory of Crop Genomics and Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China beijing key laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China

Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together.

关键词： QTLs roots wheat seedling RIL P-deficiency.

Bcl-2 and p53 expression in canine mammary tumour

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Bcl-2 and p53 expression in canine mammary tumour