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Two New Biflavonoids from the Stem of Dracaena cambodiana

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Two New Biflavonoids from the Stem of Dracaena cambodiana

海南省药学会2009年学术会议

作者： Hao-fu Dai,Jian Liu,Jiao Wu,Hui Wang,Wen-li Mei~* 1.Key Laboratory of tropical Crop biotechnology,Ministry of Agriculture,institute of tropical Bioscience and biotechnology,chinese academy of tropical agricultural sciences,haikou 571101, Hainan,P.R.china 2.International Joint Laboratory for Research and Development of tropical Medicinal Plants,chinese academy of tropical agricultural sciences,haikou 571101,china

Phytochemical studies on the stem of Dracaena cambodiana led to the discovery of two new biflavonoids,8-methylsocotrin-3’-methoxy-4’-ol(1) and 8-methylsocotrin-4’-methoxy-3’-ol(2),together with a known biflavonoid,8-methylsocotrin-4’-ol(3).Their structures were identified by means of HRESIMS and detailed spectral analysis(UV,IR,1D and 2D NMR).

关键词： Dragon’s blood Biflavonoid Dracaena cambodiana

Morphogenesis,Histology and Biochemical Characteristics of Cali Derived from Anthers of Haiken2(Hevea brasiliensis)

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Morphogenesis,Histology and Biochemical Characteristics of C...

2009整合植物生物学研讨会

作者： Deguan Tan,Meng Wang,Xiepiao Sun and Jiaming Zhang~* Key Laboratory for tropical Crops biotechnology,Ministry of Agriculture,institute of tropical Bioscience and biotechnology,chinese academy of tropical agricultural sciences, haikou 571101,china.

The morphology and histology and biochemical characteristics of cali,derived from anthers of Hevea brasiliensis M(u|¨)*** Haiken2,was systematically studied for the first *** study will be helpful in the development of tissue culture and genetic transformation of the rubber *** results showed that there were significant differences

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Chemical Composition and Anti-MRSA Activity of the Essential Oil of chinese Eaglewood

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Chemical Composition and Anti-MRSA Activity of the Essential...

第五届全国化学生物学学术会议

作者： Liu jun Cui hai-bing Mei wen-li~* institute of tropical Bioscience and biotechnology,chinese academy of tropical Agriculture sciences,haikou 571101

＜正＞Essential oil of chinese Eaglewood was hydrodistilled and characterized by GC/*** constituents comprising 66.6% of the oil were *** was found to be rich in sesquiterpenoids. Nineteen compounds comprising 45.4% were identified as ***（9.0%）, agarospirol（8.4%）,guaiol（7.8%）,aristolene（5.0%）were the major *** characteristic sesquiterpenoids of agarwood oil,such as dihydro-β-Agarofuran（1.1%）,

关键词： chinese Eaglewood essential oil composition sesquiterpenoid antibacterial activity

An Efficient Micropropagation System of Lilium longiflorum "Oriental hybrid lily"using a Two-step Explant Method

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An Efficient Micropropagation System of Lilium longiflorum "...

中国园艺学会第七届青年学术讨论会

作者： Liu Juhua, Xu Biyu, Tan Guanglan, Jin Zhiqiang (State Key Laboratory of tropical Crop biotechnology, institute of tropical Bioscience and biotechnology, chinese academy of tropical agricultural sciences, haikou, Hainan Province, 571101)

A two-step explant method combined with embryogenic callus formation which could greatly increase regeneration efficiency of Oriental hybrid lily was developed. Bulb scale segments were used as original explants and shoot sections excised from shoot clusters were used as the secondary explants. In addition, the effect of list range of 2,4-D(2,4- Dichloro-phenoxyacetic acid) concentrations and the effect of list kinds of secondary explants on callus induction have been studied. The results indicated that the propagation efficiency could be as high as 32, 750 times. The whole progress of from bulb scale to new plant formation need only 6 months. The medium supplement with 2. 0mg/L 2,4 - D in conjunction with short basal stem segment as secondary explant was most favorable for embryogenic callus formation.

关键词： Lilium longiflorum two-step explant embryogenic callus

Cloning and Sequence Analysis of Putative Promoter Regions from Ganoderma lucidum

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食用菌学报 2006年第1期 29-32页

作者： LIU Yong 1,2 , GUO Li-qiong 1,2 , LIN Jun-fang 1,2 * 1 Department of Bioengineering, College of Food sciences, South china Agriculture University,Guangzhou 510642, china 2 State Key biotechnology Laboratory for tropical Crops, chinese academy of tropical agricultural sciences, haikou 571101, china

Twelve PCR amplification products, generated by homology cloning using Ganoderma lucidum genomic DNA as the template, were cloned into the T-easy vector and sequenced. Comprehensive analysis using BLASTn, Proscan: version 1.7, and Signal Scan predicted four of the products, LY17.1, LY19.3, LY26 and LY31, to be putative promoters. Of these, LY17.1 and LY26 possessed all the promoter characteristics including promoter core regions, TATA boxes and transcription starting sites (TSS). The sequence of LY31 showed 98% homology with the gpd promoter sequence of Lentinula edodes.

关键词： Ganoderma lucidum Promoter Cloning

Characterization of genetic diversity and phylogeny relationship of wild banana species in Hainan,china

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Characterization of genetic diversity and phylogeny relation...

第十三届全国植物基因组学大会

作者： Zhiqiang Xia~1,Meiling Zou~1,Yeyuan Chen~2,Xincheng Zhou~1,Mingfu Wen~1,Wenquan Wang~(1*) 1 The institute of tropical Biosciences and biotechnology,chinese academy of tropical Agriculture sciences,haikou 571101,china 2 institute of tropical Crop Germplasm Research,chinese academy of tropical Agriculture sciences,Danzhou 571737,china

In order to assess the genetic diversity and relationship of wild species in Hainan,24 SSR primers and 14 ISSR primers were used to screen the 89 accessions among which 69 were wild species and 20 were common variety ...

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Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of the Drought-tolerance in Sugarcane

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Expression of the Grifola frondosa Trehalose Synthase Gene a...

中国生物工程学会2006年学术年会暨全国生物反应器学术研讨会

作者： Shu-Zhen ZHANG Ben-Peng YANG Cuei-Lian FENG Jing-ping LUO Wen-wei CAI Fei-hu LIU State Key Laboratory of tropical Crop biotechnology, chinese academy of tropical agricultural sciences (CATAS), haikou 571101, china institute of tropical biotechnology, CATAS, haikou 571101, china College of Life Science, Yunnan University, Kunming 650091,china

Trehalose is a nonreducing disaccharide of glucose that functions as a protectant in the stabilization of biological structures and enhances the stress tolerance under abiotic stresses in *** report here the expression of Grifola frondosa trehalose synthase (TSase )gene for improving drought tolerance in sugarcane(Saccharum officinarum L.).The expression of the

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抗冷冻蛋白基因遗传转化草菇的研究

微生物学报 2005年第1期45卷 39-43页

作者：郭丽琼林俊芳熊盛陈守才华南农业大学食品学院生物工程系广州510640 中国热带农业科学院热带作物生物技术国家重点实验室海口571101

采用RT PCR技术从瑞典的北极云杉卷叶蛾幼虫中扩增出抗冷冻蛋白基因 ,利用基因枪法遗传转化草菇。PCR检测和Southern杂交结果证明 ,抗冷冻蛋白基因已整合进草菇基因组。低温胁迫试验结果表明 ,转基因草菇具有较强的耐低温能力。转基因... 详细信息

采用RT PCR技术从瑞典的北极云杉卷叶蛾幼虫中扩增出抗冷冻蛋白基因 ,利用基因枪法遗传转化草菇。PCR检测和Southern杂交结果证明 ,抗冷冻蛋白基因已整合进草菇基因组。低温胁迫试验结果表明 ,转基因草菇具有较强的耐低温能力。转基因草菇生物学特性观测结果显示 ,大多数草菇转化子的生长速率明显地慢于对照的宿主菌株 ,多数转化子的菌丝也明显地比宿主菌丝细弱。转化子筛选结果表明 ,采用三轮的转化子筛选程序 ,即第一轮在固体培养基上筛选、第二轮和第三轮在液体培养基中筛选 ,有利于获得真实转化子和淘汰假转化子。转基因草菇一代低温胁迫结果证明 ,转基因草菇后代仍然具有较强的低温耐受能力。

关键词：草菇抗冷冻蛋白基因转化基因枪法

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鸡屎藤叶中挥发油的超临界萃取及气相色谱-质谱分析

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食品科技 2009年第12期34卷 303-304,307页

作者：尹桂豪王明月曾会才中国热带农业科学院分析测试中心海口571101 中国热带农业科学院热带生物技术研究所海口571101

采用超临界CO2萃取鸡屎藤的挥发性成分,经毛细管气相色谱-质谱联用仪(GC-MS)分析,鉴定了其中的15种挥发性化合物,用气相色谱峰面积归一法测定各组分的相对百分含量。其主要成分为植物醇31.9%、角鲨烯26.4%、亚麻酸17.6%、花生酸甲酯8.96... 详细信息

采用超临界CO2萃取鸡屎藤的挥发性成分,经毛细管气相色谱-质谱联用仪(GC-MS)分析,鉴定了其中的15种挥发性化合物,用气相色谱峰面积归一法测定各组分的相对百分含量。其主要成分为植物醇31.9%、角鲨烯26.4%、亚麻酸17.6%、花生酸甲酯8.96%、棕榈酸7.41%、豆甾醇5.42%等。为进一步开发利用鸡屎藤资源提供了科学依据。

关键词：鸡屎藤挥发油超临界CO2萃取气相色谱-质谱

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