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Acta Biochimica et Biophysica Sinica 2005年第4期37卷 234-240页

作者： Jin-Guo DING~(1,3. Jian FEI~2 Da-Fu CUI~1 You-Shang ZHANG~1 ~1institute of Biochemistry and Cell Biology,and ~2Model Organism Research Center,Shanghai institutes for Biologicol sciences,{3.,Shanghai 20003.,china ~3.raduate School of the{3.,Shanghai 20003.,china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Graduate School of the chinese academy of sciences Shanghai 20003. China Model Organism Research Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Monomeric B27 Lys destripeptide insulin (B27 Lys DTrI) was designed and produced from itsprecursor expressed in Pichia pastoris through tryptic hydrolysis instead of the less efficient *** monomeric B27 Lys DTrI precursor (MIP) was purified from a cultured medium *** by a combination of hydrophobic,size-exclusion,and ion-exchange *** purifiedMIP was converted,by tryptic hydrolysis,to B27 Lys DTrI,which was then purified by ion-exchangechromatography to homogeneity as assessed by native gel electrophoresis,HPLC,amino acid composition,and electrospray mass-spectrometric analysis.B27 Lys DTrI exhibited superior monomeric properties insize-exclusion *** yield of MIP was 200 mg per liter of culture,and the overall yield ofpurified B27 Lys DTrI from the crude MIP was 70%.The in vivo biological activity of B27 Lys DTrI asdetermined by the mouse convulsion assay was 21 U/mg,identical to that obtained by semisynthesis.

关键词： monomeric B27 Lys destripeptide insulin tryptic hydrolysis Pichia pastoris

The identity of some taxa of Schizophragma (Saxifragaceae)

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Journal of Systematics and Evolution 2009年第2期47卷 151-161页

作者： 1,2Wei LIU 1Xiang-Yun ZHU 1(institute of Botany,chinese academy of {3.,Beijing 100093.china) 2(Graduate University of chinese academy of sciences,Beijing 100049,china) institute of Botany Chinese Academy of Sciences Beijing 100093 China Graduate University of chinese academy of sciences Beijing 100049 China

After examining a large number of type and authoritative specimens of Schizophragma Siebold & Zucc.,*** *** and *** *** Rehder were reduced to synonyms of *** Rehder and *** *** *** was reduced to synonym of *** *** crassum *** *** was treated as the correct name of *** ***(Chun) *** name *** *** Rehder is *** taxonomic treat-ment is supported by leaf epidermal characters.

关键词： leaf epidermis new synonym reinstatement Schizophragma

Morphological characteristics of leaf epidermis and size variation of leaf,flower and fruit in different ploidy levels in Buddleja macrostachya (Buddlejaceae)

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Journal of Systematics and Evolution 2009年第3期47卷 231-236页

作者： 1,2Gao CHEN 1Wei-Bang SUN 3.ang SUN 1(Kunming Botanical Garden, Kunming institute of Botany, chinese academy of sciences, Kunming 650204, china) 2(Graduate School of chinese academy of sciences, Beijing 100049, china) 3.Key Laboratory of Biodiversity and Biogeography, Kunming institute of Botany, chinese academy of sciences, Kunming 650204, china) (Kunming Botanical Garden Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Graduate School of chinese academy of sciences Beijing 100049 China) (Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China)

Buddleja macrostachya (Buddlejaceae) is a widespread shrub native to the Sino-Himalayan mountains and beyond. It has been found to occur at two ploidy levels, hexaploid, 2n=6x=114 and dodecaploid, 2n=12x=228. To determine if morphological characters might be used as indicators of ploidy levels, we measured floral and fruit length, relative and absolute leaf size, trichome density on both leaf surfaces, and stomatal density and length in different populations of B. macrostachya. In general, flower and fruit length, absolute leaf size, and stomatal length increased with an increase at ploidy level (P0.05) in different populations. Other characters studied such as trichome type, cuticular membrane and ornamentation of stomata, cell and stomatal shape, and anticlinal wall pattern were quite constant in this species. Thus it appears that flower and fruit length, absolute leaf size, and stomatal frequency and length can be used to distinguish hexaploid from dodecaploid cytotypes either in the fleld or in herbarium specimens.

关键词： Buddleja macrostachya dodecaploid hexaploid leaf epidermis ploidy level stomata.

Integrated precise orbit determination of Shenzhou IV unmanned spacecraft

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Science china(Technological sciences) 2004年第5期47卷 518-525页

作者： L Congmin1,2, GU Yidong3. LIN Baojun3.& GUO Jiong3.1. Center for Space Science and Applied Research, chinese academy of sciences, Beijing 100080, china 2. Graduate School of the chinese academy of sciences, Beijing 10003., china 3. General Establishment of Space Science and Application, chinese academy of sciences, Beijing 100080, china Correspondence should be addressed to L Congmin Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China Graduate School of the chinese academy of sciences Beijing China General Establishment of Space Science and Application Chinese Academy of Sciences Beijing China

Considering the characteristic orbit of Shenzhou IV spacecraft and the advantages and disadvantages of several types of domestic orbit measurements, a scheme of IPOD is determined, which uses GPS, SLR and USB observation data jointly plus precise ionosphere corrections to GPS observation data. IPOD system and mathematic models of OD are introduced. The results of different joint OD are analyzed using true observation data, and show that radial accuracy is better than 2 m.

关键词： Shenzhou IV unmanned spacecraft integrated precise orbit determination GPS SLR USB.

Application of asymptotic waveform approximation technique to hybrid FE/BI method for 3. scattering

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Science in china(Series F) 2007年第1期50卷 124-134页

作者： PENG Zhen1,2,3.& SHENG XinQing2 1 institute of Electronics, chinese academy of sciences, Beijing 100080, china 2 Department of Electronic Engineering, Beijing institute of Technology, Beijing 100081, china 3.Graduated University of chinese academy of sciences, Beijing 10003., china institute of Electronics Chinese Academy of Sciences Beijing China Department of Electronic Engineering Beijing Institute of Technology Beijing China Graduated University of chinese academy of sciences Beijing China

The asymptotic waveform evaluation （AWE） technique is a rational function approximation method in computational mathemetics, which is used in many appllcations in computational electromagnetlca. In this paper, the performance of the AWE technique in conjunction with hybrid finite element/boundary integral （FE/BI） method is firstly Inveetlgeted. The formuletlon of the AWE applied in hybrid FE/BI method is given in detail. The characteristic implementation of the application of the AWE to the hybrid FE/BI method is discussed. Numarlcel results demonstrate that the AWE technique can greatly speed up the hybrid FE/BI method to acquire wide-band and wide-angle backecatter radar-cross-section （RCS） by complex targets.

关键词： hybrid FE/BI AWE electromagnetic scattering RCS

The effects of K^+ growth conditions on the accumulation of cesium by the bacterium Thermus sp. TibetanG6

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Science china(Life sciences) 2006年第2期49卷 123-129页

作者： WANG Hailei1,2,3. KONG Fanjing1,2,3.& ZHENG Mianping1,2,3.1. R&D Center for Saline Lake and Epithermal Deposits, chinese academy of Geological sciences, Beijing 10003.,{3. 2. Key Open Laboratory of Saline Lake Resources and Environment, chinese academy of Geological sciences, Beijing 10003.,{3. 3. institute of Mineral Resources, chinese academy of Geological sciences, Beijing 10003.,{3. R&D Center for Saline Lake and Epithermal Deposits Chinese Academy of Geological Sciences Beijing China Key Open Laboratory of Saline Lake Resources and Environment Chinese Academy of Geological Sciences Beijing China institute of Mineral Resources Chinese Academy of Geological Sciences Beijing China

The accumulation of cesium by the bacterium Thermus sp. TibetanG6 was examined under different K+ growth conditions. The effects of external pH and Na+ on the accumulation of ce-sium were also studied, and the mechanism involved was discussed. K+ regimes played an important role in the accumulation of cesium by the strain TibetanG6. The quantity of cesium accumulated (24 h) was much higher in K+-deficient regime than that in K+-sufficient regime. The pH and Na+ had different effects on the accumulation of cesium in the two K+ regimes. IR spectra analyses indicated that the biosorption is a process of homeostasis with cesium initially accumulated on the cell wall.

关键词： Thermus sp. TibetanG6, cesium, bioaccumulation, K+-growth condition, pH, sodium, IR spectroscopy.

High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2#, Libin DENG1#, and Changqing ZENG1,2* The institute of Genetic and Developmental Biology, the chinese academy of sciences, Beijing 100101, china 2Beijing Genomics institute, the chinese academy of sciences, Beijing 1013.0, china The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China Beijing Genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 24–3. bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3.were genotyped in a sample of 90 individuals, 4792 sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

Potential land for plantation of Jatropha curcas as feedstocks for biodiesel in china

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Science china Earth sciences 2010年第1期53卷 120-127页

作者： WU WeiGuang 1,2,3.HUANG JiKun 1,3.& DENG XiangZheng 1,3.1 institute of Geographic sciences and Natural Resources Research,chinese {3. of sciences,Beijing 100101,china 2 Department of Economics and Management,Zhejiang Forestry University,Linan 3.13.0,china 3.Center for chinese Agricultural Policy,chinese {3. of sciences,Beijing 100101,china institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences Beijing China Department of Economics and Management Zhejiang Forestry University Linan China Center for chinese Agricultural Policy Chinese Academy of Sciences Beijing China

As a renewable energy,biofuel has attracted great attention in china and the rest of *** with the national food security,china recently has shifted its biofuel development priority from grain-based to non-grain-based biofuels,including forest-based biodiesel,since *** curcas is one of major biodiesel ***,there is rising debate on availability of land for expanding Jatropha curcas *** overall goal of this paper is to evaluate potential land for Jatropha curcas used as feedstock for biodiesel in *** on remote sensing data on land use,data on meteorological,soil and land slope,and suitable environment for Jatropha curcas plantation,this study uses Agro Ecological Zone method and considers social-economic constraints to evaluate potential suitable land for Jatropha curcas plantation in china's major Jatropha curcas production region,Southwest *** results show that while there are some potential lands to expand Jatropha curcas areas,amount of these lands will hardly meet the government's target for Jatropha curcas-based biodiesels development in the *** may need to reconsider its long-term targets on the development of Jatropha curcas-based biodiesels.

关键词： biodiesel Jatropha curcas potential land suitability evaluation Southwest china

MODELLING AND NUMERICAL SOLUTIONS OF A GAUGE PERIODIC TIME DEPENDENT GINZBURG-LANDAU MODEL FOR TYPE-Ⅱ SUPERCONDUCTORS

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Journal of Computational Mathematics 1997年第4期15卷 365-384页

作者： Zhi-ming Chen (institute of Mathematics, chinese academy of sciences, Beijing, china) 中科院数学所北京

In this paper we seek the solutions of the time dependent Ginzburg-Landau model for type-Ⅱ superconductors such that the associated physical observables are spatially periodic with respect to some lattice whose basic lattice cell is not necessarily rectangular. After appropriately foring the gange, the model can be formulated as a system of nonlinear parabolic partial differential equations with quasi-periodic boundary conditions. We first give some results concerning the existence, uniqueness and regularity of solutions and then we propose a semiimplicit finite element scheme solving the system of nonlinear partial dmerential equations and show the optimal error estimates both in the L2 and energy *** also report on some numerical results at the end of the paper.

关键词： Math QT MODELLING AND NUMERICAL SOLUTIONS OF A GAUGE PERIODIC TIME DEPENDENT GINZBURG-LANDAU MODEL FOR TYPE SUPERCONDUCTORS

Organic geochemical studies of sinking particulate material in china sea area Ⅱ——Geochemical significance of compositional features of ketone, aldehyde and alcohol compounds

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chinese Science Bulletin 1997年第22期42卷 1894-1898页

作者： DUAN Yi,CUl Mingzhong, MA Lanhua, SONG Jinming, ZHOU Shixin and LUO Binjie1. Lanzhou institute of Geology, chinese academy of sciences, Lanzhou 73.000, china 2. Earthquake Research institute of Lanzhou, SSB, Lanzhou 73.000, china 3. institute of Oceanology, chinese academy of sciences, Qingdao 266071, china Lanzhou institute of Geology Chinese Academy of Sciences Lanzhou China Earthquake Research institute of Lanzhou SSB Lanzhou China institute of Oceanology Chinese Academy of Sciences Qingdao China

SINKING particulate organic matter of the sea plays an important role in marine environment. Itnot only provides food for marine organisms and takes part in chemical cycling, but also pro-vides sedimentary organic matter for marine sediments. Therefore, researchers always payclose attention to the source and processes of transport and transformation of marine sinkingparticulate organic matter. Its transport process can be understood by measuring the

关键词： marine sinking paniculate material composition of lipid compounds organic matter source and biochemical pro-cesses.