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Establishment of bovine prion peptide-based monoclonal antibodies for identifying bovine prion

Science china(Life Sciences) 2009年第8期52卷 754-760页

作者： ZHAO Li1,HOU XinSheng5,JI Rong2,HAN ChunHui2,YU XiuPing3,HONG Tao4,5 1 Department of Laboratory Science of Microbiology,School of Public Health,Shandong University,Jinan 250012,china 2 institute of nutrition and food safety,chinese center of disease prevention and control,beijing 100021,china 3 Department of Microbiology,School of Medicine,Shandong University,Jinan 250012,china 4 institute of Virus disease prevention and control,chinese center for disease control and prevention,beijing 100052,china 5 institute of Biological Science and Technology,School of Science,beijing Jiaotong University,beijing 100044,china Department of Laboratory Science of Microbiology School of Public Health Shandong University Jinan 250012 China institute of nutrition and food safety Chinese Center of Disease Prevention and Control Beijing 100021 China Department of Microbiology School of Medicine Shandong University Jinan 250012 China institute of Virus disease prevention and control Chinese Center for Disease Control and Prevention Beijing 100052 China institute of Biological Science and Technology School of Science Beijing Jiaotong University Beijing 100044 China

To obtain high titer monoclonal antibodies(McAbs) which can react with mammalian prion protein(PrP),Balb/C mice were immunized with bovine(Bo) PrP peptide(BoPrP 209-228 aa) coupled to keyhole limpet hemocyanin(KLH).The hybridoma cell lines secreting monoclonal antibodies against the peptide were established by cell fusion and *** obtained McAbs were applied to detect recombinant human,bovine and hamster PrP,cellular prion protein(PrPc) in normal bovine brain and pathogenic scrapie prion protein(PrPSc) accumulated in the medulla oblongata of bovine spongiform encephalopathy(BSE)specimen with Western blot and immunohistochemical detection,*** current procedure might offer a simple,feasible method to raise high titer antibodies for studying biological features of PrP in mammals,as well as detection of transmissible spongiform encephalopathy(TSE) and diagnosis of BSE,in particular.

关键词： prion monoclonal antibody Western blot immunohistochemistry ELISA

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Subchronic Toxicity Study on Soy Isoflavones in Rats

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Biomedical and Environmental Sciences 2009年第3期22卷 259-264页

作者： WEN-ZHONG ZHANG WEN-MING CUI XIN ZHANG WEI WANG XU-DONG JIA XIAO-PENG ZHANG NING LI institute of nutrition and food safety Chinese Center for Disease Control and Prevention Beijing 100021 China

Objective To investigate the subchronic toxicity of soy isoflavones （SIF） in male rats. Method Fifty Sprague-Dawley rats were randomly divided into 5 groups, 10 rats per group. SIF were given to rats in different groups by gavage at dose of 0, 0.2, 0.5, 1.5, and 4.5 g/kg bw, respectively for 13 weeks. Clinical manifestations, body weight, and food consumption were observed weekly. At the end of the study, urinalysis, hematology, clinical chemistry, total testosterone, and follicle-stimulating hormone were tested, and histopathological examinations were performed. Results No mortality, ophthalmic abnormalities or treatment-related clinical signs were identified during the study. As compared with the control group, significantly lower body weights and food consumption were observed in 1.5 and 4.5 g/kg bw groups. In clinical chemistry tests, triglyceride was significantly decreased and high-density lipoprotein cholesterol was significantly increased in all SIF-treated groups. Total testosterone levels were significantly lower in 0.50, 1.50, and 4.5 g/kg bw dose groups than in the control group. Microscopic examination showed that the mammary glands exhibited hyperplasia and excreted latex in rats of the 4.5 g/kg bw group. No changes attributable to treatment of SIF in other parameters were found. Conclusion SIF at high dosages caused significant endocrine disruption in male rats. The no observed adverse effect level （NOAEL） of SIF to male rats in this study is considered to be 0.20 g/kg bw.

关键词： Soy isoflavone Rat Subchronic toxicity

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Effects of Red Palm Oil on Serum Lipids and Plasma Carotenoids Level in chinese Male Adults

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Biomedical and Environmental Sciences 2003年第4期16卷 348-354页

作者： JIAN ZHANG , CHUN-RONG WANG, AN-NA XUE, AND KE-YOU GEinstitute of nutrition and food safety, chinese center for disease control and prevention, beijing 100050, china institute of nutrition and food safety Chinese Center for Disease Control and Prevention Beijing 100050 Chin

Objective Effects of red palm oil on major plasma carotenoids, tocopherol, retinol and serum lipids were evaluated when used in chinese diet. Methods Red palm oil group (RPO) composed of 20 male subjects(aged 18-32) and soybean oil group (SBO) composed of 22 male subjects (aged 18-32). Dietary fat provided about 28% of total calories, and the test oil accounted for about 60% of total dietary fat. In the 3 weeks of pretest period, diets were prepared with soybean oil, and then in the next 6 weeks subjects in each group consumed the diet prepared by test oil. Results Plasma α-carotene, β-carotene and lycopene concentration of RPO group significantly increased at the time of interim (21 days) and of the end (42 days) (P2 days) in this study. Though chinese plasma retinol level was relatively low when compared with that of Westerners, red palm oil diet showed no significant effect on adult chinese plasma retinol level. Serum concentration of total cholesterol, triglyceride, high density lipoprotein cholesterol, apolipoprotein AI and apolipoprotein B of all subjects showed no significant changes in RPO group during the study. Conclusions The data in our study suggest that red palm oil is a good source of carotenoids and vitamin E when used in chinese diet preparation, and it can significantly increase plasma concentration of a-carotene, α-carotene, lycopene and β-tocopherol.

关键词： Red palm oil Retinal α-carotene β-carotene α-tocopherol Serum cholesterol Serum triglyeride Apolipoprotein AI Apolipoprotein B

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Determination of 23 β_2-agonists and 5 β-blockers in animal muscle by high performance liquid chromatography-linear ion trap mass spectrometry

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Science china Chemistry 2010年第4期53卷 832-840页

作者： WU YongNing1,2, MIAO Hong1, FAN Sai1,2 & ZHAO YunFeng1,2 1 institute of nutrition and food safety, chinese Centre for disease control and prevention, beijing 100021, china 2 College of Veterinary Medicine, china Agricultural University, beijing 100193, china 1. institute of nutrition and food safety Chinese Centre for Disease Control and Prevention Beijing 100021 China2. College of Veterinary Medicine China Agricultural University Beijing 100193 China

A high performance liquid chromatography-linear ion trap mass spectrometry method using isotope dilution technique has been developed for the simultaneous determination of 23 β2-agonists and 5 β-blockers in animal muscle tissues. Pork and chicken muscle samples were acid hydrolyzed and extracted with 5% trichloracetic acid in water, and then cleaned up using MCX solid phase extraction (SPE) cartridge. Methanol and 0.1% formic acid were used as mobile phases for gradient elution. A Waters AtlantisT3 column was used for separation. ESI positive ion scan mode was used with selective reaction monitoring. 9 β2-Agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 23 β2-agonists and 5 β-blockers were 5-200 μg/L, the coefficient of correlation was not less than 0.995, and the limit of detection for each compound in the muscle tissue was below 0.2 μg/kg. The recoveries of each compound in the spiked samples at three levels 5, 10, 20 μg/kg were in the range of 47.3%-123.7%, and the relative standard deviations were in the range of 3.2%-25.7%. The developed method is sensitive and specific for the determination of β2-agonists and β-blockers in pork and chicken muscle samples.

关键词： β2-agonists β-blockers muscle determination HPLC-ITMS

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Studies on the Epidemiology and Etiology of Moldy Sugarcane Poisoning in china

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Biomedical and Environmental Sciences 1992年第2期5卷 161-177页

作者： LIU XINGJIE,LUO XUEYUN, HU WENJUANinstitute of nutrition and food Hygiene, chinese Academy of Preventive Medicine, 29 Nan Wei Road, beijing 100050, china institute of food safety control and Inspection, Ministry of Public Health, Panjiayao, beijing 100021, china. institute of nutrition and food Hygiene Chinese Academy of Preventive Medicine Beijing.

Moldy sugarcane poisoning, an acute fatal food poisoning of unknown etiology, has occurred in 13 provinces in china. The epidemiological characteristics and clinical features were described. Evidence from laboratory studies indicates that 3-nitropropionic acid roduced by the fungus Arthrinium Spp. is the etiological factor of this food poisoning.

关键词： Studies on the Epidemiology and Etiology of Moldy Sugarcane Poisoning in china

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咖啡因的科学共识

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中国食品学报 2024年第6期24卷 498-504页

作者：中国食品科学技术学会北京100048

咖啡因是一种常见于咖啡、茶叶和可可等植物中的天然活性成分,作为功能添加成分已在国际食品领域广泛应用多年,适量摄入有助于健康人群提高注意力,促进运动能力,缓解疲劳。国内外相关食品安全监管机构一致认为,咖啡因虽在日常饮食中的... 详细信息

咖啡因是一种常见于咖啡、茶叶和可可等植物中的天然活性成分,作为功能添加成分已在国际食品领域广泛应用多年,适量摄入有助于健康人群提高注意力,促进运动能力,缓解疲劳。国内外相关食品安全监管机构一致认为,咖啡因虽在日常饮食中的摄入是安全的,但需关注个体差异、摄入量以及频率。许多国家和地区已对咖啡因在食品中的应用制定了严格的应用限量和标识规定。为进一步提升大众对咖啡因的科学认知,消除对咖啡因的偏见,推动咖啡因在相关食品产业的合理应用,中国食品科学技术学会组织食品科学、公共卫生、食品安全与食品营养等领域专家与相关产业界代表,通过现场咨询、文献检索、专题研讨等形式和方法,经广泛讨论形成咖啡因的科学共识,以推动咖啡因在我国的规范使用。

关键词：咖啡因健康益处摄入量应用管理

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The Role of Sciencein Codex Standards

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Biomedical and Environmental Sciences 2001年第1期14卷 145-148页

作者： CHEN JUN SHI institute of nutrition and food Hygiene, chinese Academy of Preventive Medicine, beijing 100050, china 中国预防医学科学院营养与食品卫生所北京 100050

The key principle in the development of Codex standards is to ensure that the decision making process is based on sound science. The approval for the use of food additives and other chemicals in foods should be made only when adequate scientific data is available. The same principle applies to the development of maximum tolerable (or residue) levels for contaminants in foods. Using the General Standards for Contaminants and Toxins in foods as an example, the following criteria are considered, when developing recommendations and standards: Toxicological information; Analytical data; Intake data; Fair trade considerations; Technological considerations; and Risk assessment and risk management considerations. The Codex Alimentarius Commission has repeatedly emphasized the use of risk analysis approach, in particular the use of risk assessment in conducting the safety evaluation of food additives, agricultural and veterinary chemicals and environmental and industrial contaminants in foods. In this respect, the well known efforts of the FAO/WHO Joint Expert Committee on food Additives (JECFA) and the FAO/WHO Joint Meeting on Pesticide Residues (JMPR) provide the much needed information and recommendations for the risk assessment of specific chemicals. The risk assessment approach is also used in the safety evaluation of foods derived from modern biotechnology as well as in the quantitative assessment of microbiological risks in foods.

关键词： JECFA The Role of Sciencein Codex Standards

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The Effect of chinese Tea on Occurrence of Esophageal Tumor Induced by N-Nitrosomethylbenzylamine in Rats

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Biomedical and Environmental Sciences 1990年第1期3卷 35-42页

作者： HI AND XU YONG institute of nutrition and food Hygiene, chinese Academy of Preventive Medicine, beijing, china institute of nutrition and food Hygiene Chinese Academy of Preventive Medicine Beijing.

Five groups of rats (40 rats per group) were given five different varieties of chinese tea (1 g/50 ml) and another two groups of rats (a positive control and a negative control) were given tap water as drinking water. Except for the negative control group, all the animals were intubated with A'-nitrosomethyibenzylamine (NMBzA, 5 mg/kg body wt/week). Half of the animals in each group were sacrificed after 6 weeks and the remaining animals at the end of 12 weeks. The incidences of esophageal mucosa lesions (16-59%) at the end of6 weeks were significantly lower in the tea-treated rats than in the positive control group (100%). The incidences of esophageal tumor at the end of 12 weeks (42-67%) were also significantly lower in the tea-treated groups than in the positive control group (90%). The same phenomenon was found with respect to the size and number of tumors in each tumor-bearing animal. The relative anticarcinogenic effects of the five varieties of chinese tea were different. The Fujian oolong tea and jasmine tea exhibited the strongest effects. The results suggest that chinese tea can effectively inhibit the carcinogenesis Caused by an N-nitroso compound. 1990 Academic ***.

关键词： The Effect of chinese Tea on Occurrence of Esophageal Tumor Induced by N-Nitrosomethylbenzylamine in Rats

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The Application of NaFeEDTA Fortified Soy Sauce in the control of Iron Deficiency in china - Application phase

The Application of NaFeEDTA Fortified Soy Sauce in the Contr...

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食物营养强化营养标识及数据应用研讨会

作者： Huo Junsheng institute of nutrition and food safety chinese center for disease control and prevention

*** of *** for the Iron Fortification of Soy *** *** and Social *** of Iron Fortified Soy *** Aspect of Iron Fortified Soy *** Plan

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Large-scale Purification and Acute Toxicity of Hygromycin B PhoSphotransferase

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Biomedical and Environmental Sciences 2009年第1期22卷 22-27页

作者： QIN ZHUO,JIAN-HUA PIAO,YUAN TIAN,JIE XU,AND XIAO-GUANG YANG~2 institutefor nutrition and food safety,chinese centerfor disease control and prevention, beijing 100050,china [a]institute for nutrition and food safety Chinese Center for Disease Control and Prevention Beijing 100050 China

Objective To provide the acute toxicity data of hygromycin B phosphotransferase （HPT） using recombinant protein purified from E. coli. Methods Recombinant HPT protein was expressed and purified from E. coli. To exclude the potential adverse effect of bacteria protein in recombinant HPT protein, bacterial control plasmid was constructed, and bacteria control protein was extracted and prepared as recombinant HPT protein. One hundred mice, randomly assigned to 5 groups, were administrated 10 g/kg, 5 g/kg, or 1 g/kg body weight of HPT or 5 g/kg body weight of bacterial control protein or phosphate-buffered saline （PBS） respectively by oral gavage. Results All animals survived with no significant change in body weight gain throughout the study. Macroscopic necropsy examination on day 15 revealed no gross pathological lesions in any of the animals. The maximum tolerated dose （MTD） of HPT was 10 g/kg body weight in mice and could be regarded as nontoxic. Conclusion HPT protein does not have any safety problems to human health.

关键词： Hygromycin B phosphotransferase Selectable marker Acute toxicity safety assessment

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