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Expression of Hepatitis C Virus E2 Ectodomain in *** and Its Application in the Detection of Anti-E2 Antibodies in Human Sera

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Acta Biochimica et Biophysica sinica 2004年第1期36卷 57-63页

作者： Jing LIU, Xin-Xin ZHANG, Shen-Ying ZHANG, Min LU, Yu-Ying KONG, Yuan WANG, and Guang-Di LI ( State Key Laboratory of molecular Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, the Chinese Academy of sciences, Shanghai 200031, china 2 Department of Infectious Diseases, Ruijin Hospital, Second Medical University of Shanghai, Shanghai 200025, china ) State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China Department of Infectious Diseases Ruijin Hospital Second Medical University of Shanghai Shanghai 200025 China

The second envelope glycoprotein （E2） of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment （aa 385-730） with a four-amino-acid mutation （aa 568-571 PCNI to RVTS） was expressed as hexa-histidine-tagged full length protein [E2N730（m）] in E. coli and purified to over 85% purity. Purified E2N730（m） was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2specific antigenicity. Rabbit antiserum raised against E2N730（m） recognized E2 glyco-proteins expressed in mammalian cells in Western blot. Purified E2N730（m） was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previousl

关键词： hepatitis C virus envelope protein E2 expression and purification Escherichia coll

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Transition metal complexes bearing 2,6-bis(imino)pyridyl:Synthesis, structure, ethylene polymerization/oligomerization studies

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Science china Chemistry 2005年第Z1期48卷 45-49页

作者： SU Biyun,ZHAO Jianshe & SUN Wenhua Department of Chemistry,Northwest University,Xi’an 710069,china State Key Laboratory of Engineering Plastics and Center for molecular Science,institute of Chemistry,Chinese Academy of sciences,Beijing 100080,china Department of Chemistry Northwest University Xi’an China State Key Laboratory of Engineering Plastics and Center for molecular Science Institute of Chemistry Chinese Academy of Sciences Beijing China

A series of new complexes {2,6-bis[1-((2-methyl-4-methoxyphenyl)imino)ethyl]pyri-dine}MCI2 [M=Fe(Ⅱ) (2), Co(Ⅱ) (3), Ni(Ⅱ) (4), Cu(Ⅱ) (5), Zn(Ⅱ) (6)] have been synthesized. At 25℃, using 500 equiv of methylaluminoxane (MAO), the activities of Fe(Ⅱ), Co(Ⅱ) catalysts can reach 4.02×106 g/mol-Fehatm for ethylene polymerization and 3.98×105 g/mol-Cohatm for ethylene oligomerization. The effects of polymerization conditions such as reaction temperature, Al/M molar ratio and time on the activity of catalyst have been explored.

关键词： Bis(imino)pyridine transition metal complex, ethylene polymerization/oligomerization, catalytic activity.

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EFFECT OF PARTICLE SIZE ON THE FILMING PROCESS OF POLYSTYRENE LATEX STUDIED BY AFM

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Chinese Journal of Polymer Science 2002年第6期20卷 509-515页

作者： Xiao-zhong Qu Yi Shi Liu-sheng Chen Xi-gao Jin State Key Laboratory of Polymer Physics & Chemistry, Center for molecular Science,institute of Chemistry, Chinese Academy of sciences, Beijing 100080 china State Key Lab. of Polymer Phy./Chem. Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

The film formation process of micro-PS particles (diameter 742 nm) and nano-PS particles (diameter 29 nm) was studied by atomic force microscopy and differential scanning calorimetry. During a step heating process, the particles were annealed for 0.5 h at each selected temperature. It was found that the deformation and interdiffusion temperatures of the micro-PS particles are ca. 120-130degreesC and 140-150degreesC, that of the nano-PS particles are 90degreesC and 100-110degreesC respectively. The DSC traces of nano-PS particles showed that there was an exothermic peak near T-g after annealing for 0.5 h at the selected temperatures below 90degreesC; otherwise, the exothermic peak disappeared after annealing at 100degreesC or above. Compared with the micro-PS particles, the sintering process of nano-PS particles occurs at much lower temperature determined by the confined state of polymer chains with higher conformational energy in nano-particles, and completes in a much narrower temperature range driven mainly by the larger total surface energy.

关键词： polystyrene latex filming process particle size effect

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Diversity of symbiotic algae of the genus Symbiodinium in scleractinian corals of the Xisha Islands in the South china Sea

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Journal of Systematics and Evolution 2009年第4期47卷 321-326页

作者： ~(1,2)Zhi-Jun DONG ~1Hui HUANG~* ~1Liang-Min HUANG ~3yuan-Chao LI 1(Key Laboratory of Marine Bio-resourses Sustainable Utilization,South china Sea institute of Oceanology,Chinese Academy of sciences, Guangzhou 510301,china) 2(Yantai institute of Coastal Zone Research for Sustainable Development,Chinese Academy of sciences,Yantai 264003,china) 3(Hainan Marine Development and Design institute,Haikou 570125,china) (Key Laboratory of Marine Bio-resourses Sustainable Utilization South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China) (Yantai institute of Coastal Zone Research for Sustainable Development Chinese Academy of Sciences Yantai 264003 China) (Hainan Marine Development and Design institute Haikou 570125 China)

Symbiotic algae(Symbiodinium sp.)in scleractinian corals are important in understanding how coral reefs will respond to global climate *** present paper reports on the diversity of Syrnbiodinium sp. in 48 scleractinian coral species from 25 genera and 10 families sampled from the Xisha Islands in the South china Sea,which were identified with the use of restriction fragment length polymorphism(RFLP)of the nuclear ribosomal DNA large subunit gene(rDNA).The results showed that:(i)Symbiodinium Clade C was the dominant zooxanthellae in scleractinian corals in the Xisha Islands;(ii)Symbiodinium Clade D was found in the corals Montipora aequituberculata,Galaxea fascicularis,and Plerogyra sinuosa;and(iii)both Symbiodinium Clades C and D were found simultaneously in Montipora digitata,Psammocora contigua,and Galaxea fascicularis.A poor capacity for symbiosis polymorphism,as uncovered by RFLP,in the Xisha Islands indicates that the scleractinian corals have low adaptability to environmental *** studies are needed to investigate zooxanthellae diversity using other molecular markers.

关键词： global changes restriction fragment length polymorphism(RFLP) scleractinian coral South china Sea symbiotic algae(Symbiodinium sp.) Xisha Islands.

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Metal-amyloid-β peptide interactions: a preliminary investigation of molecular mechanisms for Alzheimer’s disease

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Science china Chemistry 2007年第4期50卷 453-467页

作者： JIAO Yong & YANG Pin Key Laboratory of Chemical Biology and molecular Engineering of Ministry of Education, institute of molecular Science, Shanxi University, Taiyuan 030006, china Key Laboratory of Chemical Biology and molecular Engineering of Ministry of Education Institute of Molecular Science Shanxi University Taiyuan China

Although humans have spent exactly 100 years combating Alzheimer’s disease (AD), the molecular mechanisms of AD remain unclear. Owing to the rapid growth of the oldest age groups of the popula-tion and the continuous increase of the incidence of AD, it has become one of the crucial problems to modern sciences. It would be impossible to prevent or reverse AD at the root without elucidating its molecular mechanisms. From the point of view of metal-amyloid-β peptide (Aβ) interactions, we review the molecular mechanisms of AD, mainly including Cu2+ and Zn2+ inducing the aggregation of Aβ, cata-lysing the production of active oxygen species from Aβ, as well as interacting with the ion-channel-like structures of Aβ. Moreover, the development of therapeutic drugs on the basis of metal-Aβ interactions is also briefly introduced. With the increasingly rapid progress of the molecular mechanisms of AD, we are now entering a new dawn that promises the delivery of revolutionary developments for the control of dementias.

关键词： Alzheimer’s disease (AD) amyloid-β peptide Cu2+ Zn2+ molecular mechanisms aggregation active oxygen species ion channel

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MONTE CARLO SIMULATION OF THE ENERGY DISSIPATION PROFILES OF 30,50 AND 100 keV INCIDENT BEAMS IN A LAYERED STRUCTURE IN ELECTRON BEAM LITHOGRAPHY

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Journal of Electronics(china) 1987年第1期4卷 46-52页

作者：孙毓平刘华 institute of Semiconductors academia sinica Chinese Science Acadeny of atomic Energies

This paper presents a Monte Carlo simulation of the energy dissipation profiles of 30, 50 and 100 ke Vincident beams in thin film (0.4μm) of electron resist, polymethyl methacrlate (PMMA), on thick siliconsubstrate in electron beam lithography, The radial scattering and the energy loss of incident electeons(including backscattered electrons from the substrate) are simulated under the illumination of ideal pointsource and Gaussian round beam spot source, and the histories of 30000--50000 electrons are computed.

关键词： EBL MONTE CARLO SIMULATION OF THE ENERGY DISSIPATION PROFILES OF 30,50 AND 100 keV INCIDENT BEAMS IN A LAYERED STRUCTURE IN ELECTRON BEAM LITHOGRAPHY PMMA

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Comparative Analysis of Two-component Signal Transduction System in Two Streptomycete Genomes

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Acta Biochimica et Biophysica sinica 2007年第5期39卷 317-325页

作者： WU WEI~(1,3#), Weihua WANG~(2#), Zhiwei CAO~(3#), Hong YU~3, Xiaojing WANG~1, Jing ZHAO~3, Hao TAN~3, Hao XU~3, Weihong JIANG~(2*), and Yixue LI~(1,3*) 1 Bioinformation Center, Key Lab of Systems Biology, Shanghai institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200031, china 2 Laboratory of molecular Microbiology, institute of Plant Physiology and Ecology, Shanghai institutes for Biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200032, china 3 Shanghai Center for Bioinformation Technology, Shanghai 200235, china Bioinformation Center Key Lab of Systems Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China Shanghai Center for Bioinformation TechnologyShanghai 200235 China Laboratory of molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200032 China

Species of the genus Streptomyces are major bacteria responsible for producing most natural antibiotics. Streptomyces coelicolor A3(2) and Streptomyces avermitilis were sequenced in 2002 and 2003, respectively. Two-component signal transduction systems (TCSs), consisting of a histidine sensor kinase (SK) and a cognate response regulator (RR), form the most common mechanism of transmembrane signal transduction in prokaryotes. TCSs in S. coelicolor A3(2) have been analyzed in detail. Here, we identify and classify the SK and RR of S. avermitilis and compare the TCSs with those of S. coelicolor A3(2) by computational approaches. Phylogenetic analysis of the cognate SK-RR pairs of the two species indicated that the cognate SK-RR pairs fall into four classes according to the distribution of their orthologs in other organisms. In addition to the cognate SK-RR pairs, some potential partners of non-cognate SK-RR were found, including those of unpaired SK and orphan RR and the cross-talk between different components in either strain. Our study provides new clues for further exploration of the molecular regulation mechanism of streptomycetes with industrial importance.

关键词： Streptomyces two-component system cross-talk phylogenetic analysis

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Evaluation of 10 plant barcodes in Bryophyta (Mosses)

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Journal of Systematics and Evolution 2010年第1期48卷 36-46页

作者： 1,2 Yan LIU 3 Hai-Fei YAN 4 Tong CAO 3 Xue-Jun GE 1(institute of Applied Ecology, Chinese Academy of sciences, Shenyang 110016, china) 2(Graduate University of the Chinese Academy of sciences, Beijing 100049, china) 3(Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South china Botanical Garden, Chinese Academy of sciences, Guangzhou 510650, china) 4(College of Life & Environment sciences, Shanghai Normal University, Shanghai 200234, china) (institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China) (Graduate University of the Chinese Academy of sciences Beijing 100049 China) (Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (College of Life & Environment sciences Shanghai Normal University Shanghai 200234 China)

DNA barcoding is a molecular tool that uses a standardized DNA region to identify *** preliminary study reported here is the first attempt to specifically focus on universality and attributes of candidate barcodes across a wide systematic range of *** tested eight previously proposed plant barcoding regions(atpF-atpH, ITS2, matK, psbK-psbI, rbcL, rpoB, rpoC1, and trnH-psbA) and two popular phylogenetic markers(rps4 and trnL-trnF of cpDNA) in 49 moss species and 9 liverwort species, representing half of the orders in moss *** ITS2, rbcL, rpoC1, rps4, trnH-psbA and trnL-trnF regions showed good universality, and therefore the efficacy of these loci as DNA barcodes was further evaluated in 36 mosses and 2 liverworts, each of which included two to three individuals per *** five loci, ***, rpoC1, rps4, trnH-psbA and trnL-trnF, were easy to amplify and sequence and showed significant inter-specific genetic variability, making them potentially useful DNA barcodes for *** best performing single loci were the rbcL and rpoC1 coding *** loci showed equivalent performance and combinations of them did not greatly increase their discrimination *** addition, phylogenies generated from each of the separate regions and multi-locus combinations by using best-fit and Kimura 2-parameter models were compared, but no significant difference was found.

关键词： Bryophyta DNA barcoding rbcL rpoC1 rps4 trnH-psbA trnL-trnF.

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Investigation of changes in properties of water under the action of a magnetic field

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Science china(Physics,Mechanics & Astronomy) 2008年第11期51卷 1621-1632页

作者： PANG XiaoFeng1,2 & DENG Bo1 1 institute of Life Science and Technology, University of Electronic Science and Technology of china, Chengdu 610054, china 2 International Centre for Materials Physics, Chinese Academy of sciences, Shenyang 110015, china institute of Life Science and Technology University of Electronic Science and Technology of China Chengdu China International Centre for Materials Physics Chinese Academy of Sciences Shenyang China

The properties of water and their changes under the action of a magnetic field were gathered by the spectrum techniques of infrared, Raman, visible, ultraviolet and X-ray lights, which may give an insight into molecular and atomic structures of water. It was found that some properties of water were changed, and a lot of new and strange phenomena were discovered after magnetization. Magnetized water really has magnetism, which has been verified by a peak shift of X-ray diffraction of magnetized water + Fe3O4 hybrid relative to that of pure water + Fe3O4 hybrid, that is a saturation and memory effect. The properties of infrared and ultraviolet absorptions, Raman scattering and X-ray diffraction of magnetized water were greatly changed relative to those of pure water; their strengths of peaks were all increased, the frequencies of some peaks did also shift, and some new peaks, for example, at 5198, 8050 and 9340 cm?1, occurred at 25°C after water was magnetized. In the meanwhile, the magnetized effects of water are related to the magnetized time, the intensity of an externally applied magnetic field, and the temperature of water, but they are not a linear relationship. The study also showed a lot of new and unusual properties of magnetized water, for example, the six peaks in 3000–3800 cm?1 in infrared absorption, the exponential increase of ultraviolet absorption of wave with the decreasing wavelength of light of 200–300 nm, the frequency-shifts of peaks, a strange irreversible effect in the increasing and decreasing processes, as well as a stronger peak of absorption occurring at 50°C, 70°C and 80°C, the existence of many models of motion from 85°C to 95°C in 8000–10000 cm?1, and so on. These results show that the molecular structure of water is very complicated, which needs further study. Furthermore, the macroscopic feature of mechanics, for instance, surface tension force of magnetized water, was also measured. Experiments discovered that the size in contact angle

关键词： pure water magnetized water magnetic-field physical property spectrum saturation and memory effect mechanical feature surface tension force polarization angles of contact

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FTIR Spectroscopic Study of Crystallization of Uniaxial Drawn Syndiotactic Polystyrene/Carbon Nanotube Nanocomposite Films

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Chemical Research in Chinese Universities 2012年第1期28卷 153-155页

作者： ZHANG Jin-hao1,2, YANG Ji-ping1, YUAN Cui-ping1,2 and CHEN Guang-ming2 1. School of Materials Science and Engineering, Beihang University, Beijing 100191, P. R. china 2. Beijing National Laboratory for molecular sciences(BNLMS), institute of Chemistry, Chinese Academy of sciences, Beijing 100190, P. R. china School of Materials Science and Engineering Beihang University Beijing 100191 institute of Chemistry Chinese Academy of Sciences Beijing National Laboratory for Molecular Sciences (BNLMS) Beijing 100190

The crystallization of uniaxial hot drawn syndiotactic polystyrene/multi-walled carbon nanotube （sPS/MWCNT） nanocomposite films was studied by FTIR spectroscopy. The effects of MWCNT content, draw ratio and drawing temperature on the sPS crystallinity were investigated. The sPS/MWCMT nanocomposite films show reduced crystallinities with the increase of MWCNT content. In addition, with the increase of draw ratio, both the pure sPS and the sPS/MWCNT nanocomposite drawn films exhibit increased crystallinity. The effect of drawing temperature on the sPS crystallization is complex. In a temperature range of 100―135 °C, the crystallinity decreases with drawing temperature, whereas it increases at 140 °C for both pure sPS and its nanocomposite films.

关键词： Carbon nanotube Crystallization FTIR spectroscopy Nanocomposite Syndiotactic polystyrene

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