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Cell Research 2004年第1期14卷 27-33页

作者： Hai Kuo ZHANG~1, Xin ZHANG~1, Bi Zeng MAO~(2,) Qun LI~1, Zu Hua HE~(1,2*) ~1SHARF and National Laboratory of Plant molecular Genetics, Shanghai institute of Plant Physiology and Ecology, Shanghai institutes for Biological sciences, Chinese Academy of sciences, Shanghai 200032, china. ~2Biotechnology institute, Zhejiang University, Hangzhou 310029, china. SHARF and National Laboratory of Plant molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Biotechnology institute Zhejiang University Hangzhou China

Alpha-picolinic acid (PA), a metabolite of tryptophan and an inducer of apoptosis in the animal cell, has been reported to be a toxin produced by some of plant fungal pathogens and used in screening for disease resistant mutants. Here, we report that PA is an efficient apoptosis agent triggering cell death of hypersensitive-like response in planta. Confirmed by Fluorescence Activated Cell Sorter (FACS), rice suspension cells and leaves exhibited programmed cell death induced by PA. The PA-induced cell death was associated with the accumulation of reactive oxygen species that could be blocked by diphenylene iodonium chloride, indicating that the generation of reactive oxygen species was NADPH-oxidase dependent. We also demonstrated the induction of rice defense-related genes and subsequent resistant enhancement by PA against the rice blast fungus Magnaporthe grisea. Hence, it was concluded that the PA-stimulated defense response likely involves the onset of the hypersensitive response in rice, which also

关键词： picolinic acid cell death hypersensitive responses Magraporche grisea rice.

A MATHEMATICAL EXPRESSION OF CRYSTAL GROWTH RATE DURING CRYSTALLIZATION OF AMORPHOUS ALLOYS

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Acta Metallurgica sinica(English Letters) 1992年第7期5卷 33-37页

作者： LU Ke WANG Jingtang, National Laboratory of RSA, institute of Metal Research, academia sinica, Shenyang, china LU Ke, Associate Professor, institute of Metal Research, academia sinica, Shenyang 110015, china

A mathematical expression of the crystal growth rate during crystallization of the amorphous alloys was derived from the micromechanism of crystallization newly developed by the authors. Thus, the satisfactory explanation of the experimental results obtained by Nunogaki et al., Heimendahl et al. and the authors might be found. It seems also to be modelled with the expression for the crystal growth and the crystal size influenced by time during the crystallization of amorphous Ni-P alloy foil at in situ heating. Based on the expression, the factors influencing the crystal growth rate, such as temperature, time and microstructure of amorphous alloys have been discussed.

关键词： amorphous alloy crystal growth rate ordered cluster shearing deposition atomic diffusion

Experimental realization of quantum cryptography communication in free space

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Science china(Physics,Mechanics & Astronomy) 2005年第2期48卷 237-246页

作者： WANG Chuan1, ZHANG Jingfu1, WANG Pingxiao1, DENG Fuguo1, AI Qing1 & LONG Guilu1,2 1. Department of Physics, Key Laboratory for Quantum Information and Measurements, Tsinghua Univer- sity, Beijing 100084, china 2. Center for atomic and molecular Nanosciences, Tsinghua University, Beijing 100084, china Department of Physics Key Laboratory for Quantum Information and Measurements Tsinghua University Beijing China atomic and molecular Nanosciences Tsinghua University Beijing China

Utilizing linear optical devices, the principle of B92 quantum key distribution (QKD) protocol is demonstrated in free space with a distance of transmission of 2.2 meters. The faint laser pulses with 650 nm wavelength are used as the single photon sources. The experimental results show that the eavesdropping behavior in the signal transmission can be detected. We also discuss the problems and solutions in using the quantum cryptography communication practically. It is pointed out that one of the approaches to increasing the distance of the quantum communication is to overcome the attenuation of the single photon in transmission. This could not be solved by the use of single photon source, and new quantum communication protocols are needed to solve these problems.

关键词： quantum key distribution, free space, linear optics, faint pulses.

JNK pathway:diseases and therapeutic potential

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Acta Pharmacologica sinica 2007年第5期28卷 601-608页

作者： Jie CUI~(2,3),Ming ZHANG~(2,3),Yong-qing ZHANG~2,Zhi-heng XU~(2,4)~2Laboratory of molecular and Developmental Biology,institute of Genetics and Developmental Biology and ~3Graduate School,ChineseAcademy of sciences,Beijing 100101,china Laboratory of molecular and Developmental Biology Institute of Genetics and Developmental Biology Beijing China Graduate School Chinese Academy of Sciences Beijing China

c-Jun N-terminal protein kinases (JNK),also known as stress-activated proteinkinases,were originally identified by their ability to phosphorylate the N-terminalof the transcription factor c-Jun and by their activation in response to a variety *** are multifunctional kinases involved in many *** JNK pathway has been shown to play a major role in apoptosis inmany cell death paradigms and its association with a variety of pathological pro-cesses is gradually been *** review will concentrate on describingthe involvement of the JNK pathway in the context of different diseases and thepotential to adopt the JNK pathway components as therapeutic targets.

关键词： JNK pathway signal transduction diseases inhibitors

Isospin-dependent relativistic microscopic optical potential in the Dirac Brueckner-Hartree-Fock method

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Science china(Physics,Mechanics & Astronomy) 2004年第2期47卷 189-198页

作者： RONG Jian1 & MA Zhongyu1,2,3 1. china institute of atomic Energy, Beijing 102413, china 2. Center of Nuclear Theoretical Physics, National Laboratory of Heavy Ion Accelerator of Lanzhou, Lan-zhou 730000, china 3. institute of Theoretical Physics, Chinese Academy of sciences, Beijing 100080, china Correspondence should be addressed to Rong Jian (email: jrong@***) china institute of atomic Energy Beijing China Center of Nuclear Theoretical Physics National Laboratory of Heavy Ion Accelerator of Lanzhou Lanzhou China institute of Theoretical Physics Chinese Academy of Sciences Beijing China

The relativistic microscopic optical potential in the asymmetric nuclear matter is studied in the framework of the Dirac Brueckner-Hartree-Fock method. A new decomposition of the Dirac structure of the nuclear self-energy in nuclear matter is adopted. The self-energy of a nucleon with E > 0 in nuclear matter is calculated with the G matrix in the Hartree-Fock approach. The optical potential of a nucleon in the nuclear medium is identified with the nucleon self-energy. The energy and asymmetric parameter dependence of the relativistic optical potentials for proton and neutron are discussed. The resulting Schroedinger equivalent potentials have reasonable behaviors of the energy dependence. The asymmetric parameter dependence of relativistic optical potentials and Schroedinger potentials are emphasized.

关键词： Dirac Brueckner Hartree-Fock, relativistic microscopic optical potential, asymmetric nuclear matter, isospin-dependence.

SPECTRAL APPROXIMATION THEORY FOR MULTIGROUP NEUTRON TRANSPORT OPERATORS

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Acta Mathematica Scientia 1986年第3期6卷 339-352页

作者：宋予合阳名珠 institute of atomic Energy academia sinica

In this paper, by using operator theory in Banach space, we mainly study spectral approximation theory for the case of an inhomogeneous, anisotropically scattering, and arbitrarily finite convex body with any cavity in an L~p context for generall p,1≤p<∞. We prove that solutions of the time-dependent multigroup systems converge, in a suitable sense, to the corresponding solution of the exact time-dependent transport equation; the eigenvalues and the corresponding eigenfunctions and generalized eigenfunctions of the exact transport operator are approximated respectively by the eigenvalues and the corresponding eigenfunctions and generalized eigenfunctions of the multigroup transport operotors; the dominant eigenvalues and the corresponding positive eigenfunctions of the multigroup transport operators (the existence is proved, too) accumulate to those of the exact transport operators; the orders of magnitude of the rate of convergence are given respectively. Other results are obtained to compare the dim

关键词： SPECTRAL APPROXIMATION THEORY FOR MULTIGROUP NEUTRON TRANSPORT OPERATORS

Analysis of the Interaction between hPFTAIRE1 and PLZF in a Yeast Two-hybrid System

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Acta Biochimica et Biophysica sinica 2006年第3期38卷 164-170页

作者： Yan-Kun GAO~# Mei JIANG~# Tao YANG Jiang-Ye CHEN~* State Key Laboratory of molecular Biology,institute of Biochemistry,and Cell Biology,Shanghai institutes for Biological sciences,Chinese Academy of sciences,Shanghai 200031,china State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

hPFTAIRE1 is a Cdc2-related kinase family *** search its substrates and regulatoryproteins,hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid *** this screening,seven hPFTAIRE1 interacting proteins,including promyelocytic leukemia zinc finger (PLZF),were *** interaction between PLZF and hPFTAIRE1 was confirmed by β-galactosidase assay andLeu growth *** encodes a transcription factor belonging to the POZ/BTB domain and Krüppelzinc finger (POK) *** highly conserved POZ/BTB domain plays a critical role in *** deleted the POZ/BTB and Krüppel zinc finger domains,respectively,and observed the inter-action between hPFTAIRE1 and truncated PLZFs by liquid β-galactosidase activity assay.A weak interactionwas detected between hPFTAIRE1 and *** also observed the interaction between PLZF and anotherCdc2-related kinase,PCTAIRE1.A similar result was *** interaction between PLZF and hPFTAIRE1or PCTAIRE1 was confirmed by co-immunoprecipitation assay in a yeast *** is a phosphoproteinand plays multiple roles during cell *** results suggest that hPFTAIRE1 and PCTAIRE1 may playimportant roles in the functional regulation of PLZF.

关键词： hPFTAIRE1 PLZF PCTIARE1 yeast two-hybrid interaction

β-delayed proton decays near the proton drip line

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Science china(Physics,Mechanics & Astronomy) 2005年第3期48卷 257-267页

作者： XU Shuwei 1 , LI Zhankui 1 , XIE Yuanxiang 1 , HUANG Wenxue 1 , SHU Nengchuan 2 , CHEN Yongshou 2 , XU Furong 3 , WANG Kun 4 , PAN Qiangyan 1 , WANG Xudong 1 , YU Yong 1 & XING Yebing 1 1. institute of Modern Physics, Chinese Academy of sciences, Lanzhou 730000, china 2. Nuclear Physics Department, china institute of atomic Energy, Beijing 102413, china 3. Department of Technical Physics, Peking University, Beijing 100871, china 4. Shanghai institute for Nuclear Research, Shanghai 201800, china institute of Modern Physics Chinese Academy of Sciences Lanzhou China Nuclear Physics Department China Institute of Atomic Energy Beijing China Department of Technical Physics Peking University Beijing China Shanghai institute for Nuclear Research Shanghai China

We briefly reviewed the experimental study on a-delayed proton decays near the proton drip line published by our group during the period of 1996―2004, namely the first observation of the a-delayed proton decays of 9 new nuclides in the rare-earth region and the new measurements of a-delayed proton decays of 5 nuclides in the mass ～90 region near the N = Z line with the aid of the “p-?” coincidence in combination with a He-jet tape transport system. In the meantime some important experimental technique details were supplemented. The experimental results, including the half -lives, spins, parities, deformations and production reaction cross sections for the 14 nuclei were summarized and compared with the current nuclear-model predictions, and then the following points were represented. (1) The experimental half-lives for 85Mo and 92Rh as well as the predicted “waiting point” nuclei 89Ru and 93Pd are 5―10 times longer than the theoretical predictions given by M?ller et al. using a macroscopic-microscopic model. It considerably influences the predictions of the abundances of the nuclides produced in the rp-process. (2) The current-model predictions are not consistent with the experimental assignments of the spins and parities for the proton drip-line nuclei 142Ho and 128Pm. However, the nuclear potential energy surface (PES) calculated by using a Woods- Saxon-Strutinsky method reproduced the experimental results. (3) The Alice code overestimated the production reaction cross sections of the studied 9 rare-earth nuclei by one order of magnitude or two, while HIVAP code overestimated them by one order of magnitude approximately.

关键词： proton drip line, β-delayed proton decay, He-jet tape transport system, "p-γ" coincidence, spin, parity, deformation, production reaction cross section.

Computational Identification of Novel Family Members of MicroRNA Genes in Arabidopsis thaliana and Oryza sativa

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Acta Biochimica et Biophysica sinica 2005年第2期37卷 7+76-87页

作者： Yang LI Wei LI You-Xin JIN State Key Laboratory of molecular Biology,institute of Biochemistry and Cell Biology.Shanghai institutes for Biological sciences,Chinese Academy of sciences.Shanghai 200031.china State Key Laboratory of molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

MicroRNAs(miRNAs)are a class of endogenous small RNAs that play important regulatoryroles in both animals and plants,miRNA genes have been intensively studied in animals,but not in *** study,we adopted a homology search approach to identify homologs of previously validated plantmiRNAs in Arabidopsis thaliana and Oryza *** identified 20 potential miRNA genes in Arabidopsisand 40 in ***,providing a relatively complete enumeration of family members for these miRNAs *** addition,a greater number of Arabidopsis miRNAs(MIR168,MIR159 and MIR172)were found tobe conserved in *** the novel homologs,most of the miRNAs have closely related fellow miRNAs andthe number of paralogs varies in the different miRNA ***,a probable functional segmenthighly conserved on the elongated stem of pre-miRNA fold-backs of MIR319 and MIR159 family *** results support a model of variegated miRNA regulation in plants,in which miRNAs withdifferent functional elements on their pre-miRNA fold-backs can differ in their function or regulation,andclosely related miRNAs can be diverse in their specificity or competence to downregulate target *** that the sophisticated regulation of miRNAs can achieve complex biological effects through qualita-tive and quantitative modulation of gene expression profiles in plants.

关键词： microRNA post-transcriptional gene silencing rice Arabidopsis

Methanol as a probe to investigate the relationship between the secondary electron donor TyrZ and the substrate water molecules in active photosystem Ⅱ

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Chinese Science Bulletin 2010年第9期55卷 809-813页

作者： BAO Han, ZHANG ChunXi, REN YaNan & ZHAO JingQuan Laboratory of Photochemistry, Beijing National Laboratory of molecular sciences, institute of Chemistry, Chinese Academy of sciences, Beijing 100190, china Laboratory of Photochemistry Beijing National Laboratory of Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing China

The secondary electron donor, TyrZ, is implicated in tuning the primary charge separation and the water oxidation in active pho-tosystem II (PSII). Two types of mechanisms have been proposed to explain the function of TyrZ. One is that TyrZ tunes the water oxidation through the direct interaction with substrate water molecules; the other is that TyrZ is located in a hydrophobic envi-ronment without interacting with H2O, and controls the water oxidation by tuning the strength of the hydrogen bond between TyrZ and His190. Here, methanol was used as a probe to study the possible relationship between TyrZ and H2O by monitoring the TyrZ oxidation and TyrZ· reduction at cryogenic temperatures with electron paramagnetic resonance spectroscopy. The oxidation of TyrZ and reduction of TyrZ· in both S2 and S0 states at 10 K were accelerated by addition of a small amount of methanol (6%). Theoretical studies indicate that Tyr oxidation becomes more difficult if it interacts directly with the methanol molecule; while the decrease of the polarity of its environment accelerates the oxidation of Tyr. Accordingly, CH3OH does not directly interact with TyrZ in active PSII, and the accelerative effect of methanol is caused by the strength increase of the hydrogen bond between TyrZ and His190, resulting from the decrease of polarity of their environment after the displacement of H2O by CH3OH inside PSII. Considering the similarity between methanol and water, the results in this study support the model in which TyrZ does not interact with H2O in active PSII.

关键词： photosystem Ⅱ secondary electron donor TyrZ methanol water electron paramagnetic resonance