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Cloning,expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco

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Progress in Natural Science:Materials International 2007年第08期17卷 906-912页

作者： Zhou Qiyun~(1,2**) Xie Zongming~(1**) Zhang Zhigang~1 Cao Yangrong~1 Zhang jinsong~(1***) Chen Shouyi~(1***) (1.National Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,chinese academy of sciences,beijing100101,china 2.graduate university of chinese academy of sciences,beijing 100039,china) [a]National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China [b]graduate university of chinese academy of sciences Beijing China

Nucleoside diphosphate kinase (NDPK) is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except *** we report that a full-length cDNA encoding nucleoside diphosphate kinase (NDPK) was cloned us- ing yeast two-hybrid approach.A tobacco NDPK gene was obtained and designated as ***1 is 704 bp in length and en- codes a putative 16.2 kD protein of 148 amino *** analysis showed that NtNDPK1 is highly homologous to other plant NDPK genes and identified as type I (NDPK1).RNA-gel blot analysis showed that there was no significant difference of NtNDPK1 ex- pression in roots,stems,leaves and *** expression of NtNDPK1 was induced by ABA and PEG and repressed by NaCl,but not significantly affected by Paraquat,wounding and low temperature (4℃) treatments,indicating that NtNDPK1 may play a certain role in response to abiotic *** vitro phosphorylation assay demonstrated that NtNDPK1 had autophosphoryLation activity.

关键词： abiotic stress autophosphorylation activity NtNDPK1 tobacco yeast two-hybrid

Nonlinear interactions between gravity waves and background winds

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Progress in Natural Science:Materials International 2007年第6期17卷 639-644页

作者： Liu Xiao1,2 and Xu Jiyao1(1. State Key Laboratory for Space Weather, Center for Space Science and Applied Research, chinese academy of sciences, beijing 100080, china 2. graduate university of chinese academy of sciences, beijing 100039, china) [a]State Key Laboratory for Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China [b]graduate university of chinese academy of sciences Beijing China

Using the nonlinear propagating gravity waves (GW) model in the two-dimensional compressible atmosphere and the linear GW theory, the process of GW propagation in different background winds, e.g. the direction of the background wind is opposite to (dead wind) or the same as (tail wind) the direction of the horizontal phase velocity of GW, is studied. The results show that the dead wind prolongs the vertical wavelength and accelerates GW propagation. Therefore, GW propagates up to a higher height becomes instable in a short time and eventually induces an inverse jet flow. Then, the vertical wavelength is becoming short due to the nonlinear interactions between GW and the inverse jet flow. The vertical wavelength and group velocity decrease after GW propagates into the tail wind. The initial instable time is delayed. Although most of GW is trapped in the instable region, some of GW propagates above the instable region. Compared with GW propagation in the tail wind, the nonlinear interactions between GW and the dead wind are also strong. In contrast, the linear GW theory predicts that GW can propagate freely in the dead wind. The vertical wavelength simulated by the nonlinear numerical model is different from that predicted by the linear theory greatly after GW propagates into the dead wind.

关键词： gravity waves background winds vertical group velocity vertical wavelength nonlinear interactions.

Biodegradable amphiphilic block copolymers containing functionalized PEO blocks:Controlled synthesis and biomedical potentials

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Science china Chemistry 2010年第3期53卷 519-527页

作者： ZHANG Yu1,2,SUN PeiJian1,2 & GAN ZhiHua1 1CAS Key Laboratory of Engineering Plastics,Institute of Chemistry,chinese academy of sciences,beijing 100190,china 2graduate university of chinese academy of sciences,beijing 100039,china 1. CAS Key Laboratory of Engineering Plastics Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China2. Graduate University of Chinese Academy of Sciences Beijing 100039 China

A series of controllable amphiphilic block copolymers composed of poly(ethylene oxide)(PEO) as the hydrophilic block and poly(ε-caprolactone)(PCL) as the hydrophobic block with the amino terminal group at the end of the PEO chain(PCL-b-PEO-NH2) were *** on the further reaction of reactive amino groups,diblock copolymers with functional carboxyl groups(PCL-b-PEO-COOH) and functional compounds RGD(PCL-b-PEO-RGD) as well as the triblock copolymers with thermosensitive PNIPAAm blocks(PCL-b-PEO-b-PNIPAAM) were *** well-controlled structures of these copolymers with functional groups and blocks were characterized by gel permeation chromatography(GPC) and 1H NMR *** copolymers with functionalized hydrophilic blocks were fabricated into microspheres for the examination of biofunctions via cell culture experiments and in vitro drug *** results indicated the significance of introducing functional groups(e.g.,NH2,COOH and RGD) into the end of the hydrophilic block of amphiphilic block copolymers for biomedical potentials in tissue engineering and controlled drug release.

关键词： amphiphilic block copolymers biodegradable polymers functional terminal group cell growth drug release

Gasdynamic characteristics of toroidal shock and detonation wave converging

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Science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 739-749页

作者： TENG Honghui1,2 & JIANG Zonglin2 1. Key Laboratory of High Temperature Gas Dynamics, Institute of Mechanics, chinese academy of Sci- ences, beijing 100080, china 2. graduate university of chinese academy of sciences, beijing 100039, china 1. Key Laboratory of High Temperature Gas Dynamics Institute of Mechanics Chinese Academy of Sciences 100080 Beijing China 2. graduate university of chinese academy of sciences 100039 Beijing China

The modified CCW relation is applied to analyzing the shock, detonation wave converging and the role of chemical reactions in the process. Results indicate that the shock wave is strengthened faster than the detonation wave in the converging at the same initial Mach number. Euler equations implemented with a detailed chemical reaction model are solved to simulate toroidal shock and detonation wave converging. Gasdynamic characteristics of the converging are investigated, including wave interaction patterns, observable discrepancies and physical phenomena behind them. By comparing wave diffractions, converging processes and pressure evolutions in the focusing area, the different effects of chemical reactions on diffracting and converging processes are discussed and the analytic conclusion is demonstrated through the observation of numerical simulations.

关键词： converging, CCW relation, shock wave, detonation wave.

Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2, Zhou Xiuxia1,2, Huang Rong1,2 and Guo Qionglin1(1. Institute of Hydrobiololgy, chinese academy of sciences, Wuhan 430072, china 2. graduate university of chinese academy of sciences, beijing 100039, china) Institute of Hydrobiololgy Chinese Academy of Sciences Wuhan 430072 China graduate university of chinese academy of sciences Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352 amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2 recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

A SAR sidelobe suppression algorithm based on modified spatially variant apodization

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Science china(Technological sciences) 2010年第9期53卷 2542-2551页

作者： NI Chong1,2,WANG YanFei1,XU XiangHui1,ZHOU ChangYi1 & CUI PengFei1,2 1 Institute of Electronics,chinese academy of sciences,beijing 100190,china 2 graduate university of chinese academy of sciences,beijing 100039,china 1. Institute of Electronics Chinese Academy of Sciences Beijing 100190 China2. Graduate University of Chinese Academy of Sciences Beijing 100039 China

The existing spatially variant apodizations (SVAs) either cannot depress the sidelobes effectively or reduce the energy of the *** improve this,a modified SVA (MSVA) is put forward in this paper,which expands the traditional filter from 3-taps to 5-taps and sets relevant parameters according to different sampling rates to get the excellent result that satisfies constrained optimization theory.A method for synthetic aperture radar (SAR) sidelobe control based on MSVA is presented,which applies MSVA to range compression and azimuth compression to control *** method which is available for any Nyquist sampling rate can both depress the sidelobes effectively and keep the energy of the mainlobe and the resolution of the *** method can reduce sidelobe levels more effectively than classical amplitude weighting while maintaining the image resolution,as demonstrated by the result of the experiment.

关键词： synthetic aperture radar sidelobe suppression resolution SVA

SHRIMP zircon U-Pb geochronology of early Mesozoic felsic igneous rocks from the southern Lancangjiang and its tectonic implications

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Science china Earth sciences 2006年第10期49卷 1032-1042页

作者： PENG Touping1,2, WANG Yuejun1, FAN Weiming1, LIU Dunyi3, SHI Yuruo3 & MIAO Laicheng4 1. Key Laboratory of Isotope Geochronology and Geochemistry, Guangzhou Institute of Geochemistry, chinese academy of sciences, Guangzhou 510640, china 2. graduate university of chinese academy of sciences, beijing 100039, china 3. SHRIMP isotope Laboratory, chinese academy of Geological sciences, beijing 100037, china 4. Institute of Geology and Geophysics, chinese academy of sciences, beijing 100029, china Key Laboratory of Isotope Geochronology and Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China graduate university of chinese academy of sciences Beijing China SHRIMP isotope Laboratory Chinese Academy of Geological Sciences Beijing China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China

The SHRIMP zircon U-Pb geochronology of three typical samples, including two monzo nitic granites from the Lincang batholith and a rhyolite from the Manghuai Formation are presented in the southern Lancangjiang, western Yunnan Province. The analyses of zircons for the biotite monzonitic granites from the northern (02DX-137) and southern (20JH-10) Lincang batholith show the single and tight clusters on the concordia, and yield the weighted mean 206Pb/238U ages of 229.4 ± 3.0 Ma and 230.4 ± 3.6 Ma, respectively, representing the crystallized ages of these granites. The zircons for the rhyolitic sample (02DX-95) from the Manghuai Formation give a weighted mean 206Pb/238U age of 231.0 ± 5.0 Ma. These data suggest that the igneous rocks from the Lincang granitic batholith and Manghuai Formation have a similar crystallized age. In combination with other data, it is inferred that both were generated at a narrow age span (~230 Ma) and were originated from the postcollisional tectonic regime. An early Proterozoic 206Pb/238U apparent age of 1977±44 Ma is additionally obtained from one zircon from the biotite monzonitic granite (southern Lincang batholith), indicative of devel- opment of the early Proterozoic Yangtze basement in the region. These precisely geochronological data provide important constraints on better understanding the Paleozoic tectonic evolution of the Tethys, western Yunnan Province.

关键词： SHRIMP zircon U-Pb dating, Middle Triassic, Lincang granitic batholith, Manghuai Formation rhyolite, southern Lancangjiang.

Photodissociation/photoionization processes of chlorobromomethane induced by femtosecond laser pulses with pump-probe scheme

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chinese Science Bulletin 2008年第5期53卷 681-686页

作者： ZHANG Feng1,2, WEI ZhengRong1,2, CAO ZhenZhou1,2, ZHANG ChangHua1,2 & ZHANG Bing1,2 1 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, chinese academy of sciences, Wuhan 430071, china 2 graduate university of chinese academy of sciences, beijing 100039, china State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Wuhan Institute of Physics and Mathematics Chinese Academy of Sciences Wuhan China graduate university of chinese academy of sciences Beijing China

The photodissociation/photoionization processes of chlorobromomethane (CH_2BrCl) induced by fem- tosecond laser pulses have been investigated using pump-probe scheme combined with the time-of-flight mass spectra. The dominate photoproducts are observed at different delay time of the pump (400 nm) and probe (800 nm) pulses and the corresponding time-dependence of them is obtained. The results show that the decaying time of the molecule CH_2BrCl in the A-band is in the 100 fs. The decaying tendencies of the fragment ions (CH_2Cl^+ and CH_2Br^+) and the parent ion (CH_2BrCl^+) are almost the same and the relative ratios of the yields of them keep constant during the delay time of 0 to 150 fs. These facts suggest that the fragment ions come from the fragmentation of the parent ions in excited electronic states. The probabilities to form CH_2Cl^+ and CH_2Br^+ are obtained from the relative ratio of the ion intensity and are about 71.6% and 14.2%, respectively.

关键词：毫微秒激光氯溴化甲烷激光探针光解作用光化电离作用

Environmental characteristics of Mid-Holocene recorded by lacustrine sediments from Lake Daihai, north environment sensitive zone, china

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Science china Earth sciences 2006年第9期49卷 968-981页

作者： SUN Qianli1,2, ZHOU Jie1, SHEN Ji3, CHEN Peng1,2, WU Feng1,2 & XIE Xiuping1,2 1. State Key Lab of Loess and Quaternary Geology, Institute of Earth Environment, chinese academy of sciences, Xi’an 710075, china 2. graduate university of chinese academy of sciences, beijing 100039, china 3. Nanjing Institute of Geography and Limnology, chinese academy of sciences, Nanjing 210008, china 1. State Key Lab of Loess and Quaternary Geology Institute of Earth Environment Chinese Academy of Sciences Xi’an 710075 China 2. graduate university of chinese academy of sciences Beijing 100039 China 3. Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China

Climate proxies, such as total organic carbon and nitrogen (TOC, TN), carbonate content (CaCO3), grain-size and pollen of the sediment core retrieved from enclosed Lake Daihai which lies in the north china environment sensitive zone are analyzed to reconstruct the environment evolution of the area based on high-resolution radiocarbon chronology. The results reveal that the TOC and TN contents of the sediments correlate well with pollen percentage and pollen flux variations during the Holocene, and both reach their peak values simultaneously at 6.7―3.5 ka BP (calendar age, 7.6―3.6 ka BP). Since 6.7 ka BP, both the CaCO3 and organic matter contents of the core have simultaneous variations, and their high values also occur during 6.7―3.5 ka BP. While during 9.0―6.7 ka BP (calendar age, 10―7.6 ka BP) relatively lower level of organic matter content and pollen flux corre- spond to the higher level of carbonate content. The above relations suggest that during 6.7―3.5 ka BP, the productivity and effective precipitation were greatly improved in the lake drainage area, and this would probably strengthen the hydrodynamic conditions, enhancing organic matter, pollen and carbonate inputs from terrestrial sources. Such processes would account for the enrichment of both organic matter and carbonate in the sediments. While during 9.0―6.7 ka BP, the lower level of or- ganic matter, pollen flux but high carbonate content show depressed productivity and declined vege- tation coverage. The higher carbonate content at this stage would have probably resulted from the higher evaporation ratio of the lake water under relatively drier climate conditions. Therefore, it is in- ferred that during 6.7―3.5 ka BP, the climate was more humid with abundant rainfalls and vegetation was more flourishing in the Lake Daihai area. This can be seen as Holocene Climate Optimum (HCO). As a result, this has evident discrepancies with the traditional notion that the HCO occurs at Early Holocene or early Mid

关键词： Lake Daihai, Mid-Holocene, lacustrine sediments, environmental characteristics.