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Experimental study on phosphorus release from sediments of shallow lake in wave flume

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Science china Earth sciences 2006年第Z1期49卷 92-101页

作者： SUN Xiaojing,ZHU Guangwei, LUO Liancong & QIN Boqiang Nanjing Institute of Geography & Limnology, chinese Academy of sciences, Nanjing 210008, china graduate school of chinese Academy of sciences, beijing 100039, china Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing China graduate school of chinese Academy of sciences Beijing China

Influence of wave on sediment resuspension and nutrients release from sediments, collected from Lake Taihu and Lake Chaohu, was studied in flume experiments. Under strong-wave conditions, concentrations of suspended solids (SS), total phosphorus (TP) and dissolved total phosphorus (DTP) in overlying water were increased significantly following the sediments re-suspension. During the experiments on sediments of Lake Taihu and Lake Chaohu, TP concentrations increased 6 times and 3 times, and DTP concentration increased 100% and 70% more than it in presuspension, respectively. Concentration of soluble reactive phosphorus (SRP) of experiment on sediment of Lake Taihu increased 25%. During the massive sediment suspension, the dissolved phosphorus in pore water and much of the phosphorus adsorbed by the sediment particles were released into overlying water. The phenomena in this wave flume experiment are quite similar to the situation observed in situ of Lake Taihu. The critical wave stresses of sediment re-suspension are nearly equal. The change of concentrations of SS, TP, and SRP was the same as that in situ *** study showed that concentrations of TP and SRP in lake water could be increased significantly by wave disturbance. Phosphorus release was significantly enhanced by wave disturbance at the beginning of massive sediment re-suspension, but decreased later.

关键词： shallow lakes, wave disturbance, internal release, phosphorus, wave flume experiment.

Identification of the Angel -related elements in cyprinid fishes and their phylogenetic implications

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Progress in Natural Science:Materials International 2007年第2期17卷 168-171页

作者： WANG Li, HE Shunping and CHEN Yiyu ( Institute of Hydrobiology, chinese Academy of sciences, Wuhan 430072, china graduate school of the chinese Academy of sciences, beijing 100039, china National Natural Science Foundation of china, beijing 100085, china) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China graduate school of the chinese Academy of sciences Beijing 100039 China National Natural Science Foundation of china Beijing 100085 China

Angel-related element belongs to the family of miniature inverted-repeat transposable elements (MITEs). In this paper we report the identification of an Angel- related element in the series Leuciscini of cyprinid fishes, which is located in the second intron of the growth hormone (GH) gene. We have also found that this element is absent in orthologous locus in the series Barbini of cyprinid fishes, that provides new evidence for the monophyly of the series Leuciscini. The insertion of Angel -related element into the GH gene might take place in the common ancestor of the series Leuciscini after its divergence from the series Barbini. The high sequence divergence and relatively broad species distribution of Angel-related elements implies that they might be ancient transposons which appeared about 26 million years ago.

关键词： Angel -related elements the series Leuciscini monophyly.

Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2, Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese Academy of sciences, beijing 100080, china 2Center of Mass Spectrometry, Institute of Chemistry, chinese Academy of sciences, beijing 100080, china 3graduate school of the chinese Academy of sciences, beijing 100039, china Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China graduate school of the chinese Academy of sciencesbeijing 100039 China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

Possible Energy Network with polygeneration system and CCS for china

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中国科学：技术科学英文版 2010年第1期53卷 33-39页

作者： GAO Lin1,LI Sheng1,2,JIN HongGuang1 & LIN Hu1 1 Institute of Engineering Thermophysics,chinese Academy of sciences,beijing 100190,china 2 graduate school of chinese Academy of sciences,beijing 100039,china Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing China graduate school of chinese Academy of sciences Beijing China

On basis of adopting polygeneration systems for power and alternative fuels,capturing CO2 with near zero energy penalties,and storing CO2 on sites,a new kind of Energy Network can integrate energy utilization,CO2 capture,transportation and storage ***-economic analysis of this solution focusing on Inner Mongolia and the Yangtze River Delta districts had been carried with comparison to the chain method for energy utilization and CO2 *** solution can save 21.5% of energy,and reduce 35% of total *** adoption of advanced polygeneration systems contributes 52.2% of the total saved costs,and the integration of energy utilization and CO2 sequestration in the Energy Network contributes 47.8%.From the aspect of CCS,the CO2 sequestration cost in the Energy Network can be as low as 12 $/t due to the lower energy penalties of capture in polygeneration systems and the combination of CO2 source and *** Energy Network exhibits attractive performance on energy saving,costs reduction for CCS,which may be a promising solution for sustainable development of china.

关键词： CO2 capture and storage Energy Network polygeneration system

Characterization of a sodium-regulated glutaminase from cyanobacterium Synechocystis sp. PCC 6803

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Science china(Life sciences) 2008年第12期51卷 1066-1075页

作者： ZHOU Jie1, ZHOU JunXia1,2, YANG HaoMeng1, YAN ChengShi1 & HUANG Fang1 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, chinese Academy of sciences, beijing 100093, china 2 graduate school of chinese Academy of sciences, beijing 100039, china Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing China graduate school of chinese Academy of sciences Beijing China

Glutaminase is widely distributed among microorganisms and mammals with important functions. Lit-tle is known regarding the biochemical properties and functions of the deamidating enzyme glutami-nase in cyanobacteria. In this study a putative glutaminase encoded by gene slr2079 in Synechocystis sp. PCC 6803 was investigated. The slr2079 was expressed as histidine-tagged fusion protein in Es-cherichia coli. The purified protein possessed glutaminase activity, validating the functional assign-ment of the genomic annotation. The apparent Km value of the recombinant protein for glutamine was 26.6 ± 0.9 mmol/L, which was comparable to that for some of other microbial glutaminases. Analysis of the purified protein revealed a two-fold increase in catalytic activity in the presence of 1 mol/L Na+. Moreover, the Km value was decreased to 12.2 ± 1.9 mmol/L in the presence of Na+. These data demon-strate that the recombinant protein Slr2079 is a glutaminase which is regulated by Na+ through in-creasing its affinity for substrate glutamine. The slr2079 gene was successfully disrupted in Synecho-cystis by targeted mutagenesis and the △slr2079 mutant strain was analyzed. No differences in cell growth and oxygen evolution rate were observed between △slr2079 and the wild type under standard growth conditions, demonstrating slr2079 is not essential in Synechocystis. Under high salt stress condition, however, △slr2079 cells grew 1.25-fold faster than wild-type cells. Moreover, the photosyn-thetic oxygen evolution rate of △slr2079 cells was higher than that of the wild-type. To further charac-terize this phenotype, a number of salt stress-related genes were analyzed by semi-quantitative RT-PCR. Expression of gdhB and prc was enhanced and expression of desD and guaA was repressed in △slr2079 compared to the wild type. In addition, expression of two key enzymes of ammonium assimi-lation in cyanobacteria, glutamine synthetase (GS) and glutamate synthase (GOGAT) was examined by semi-quant

关键词： cyanobacteria Synechocystis putative glutaminase enzyme activity mutagenesis salt tolerance

Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

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Progress in Natural Science:Materials International 2005年第2期15卷 54-61页

作者： XIA Junhong 1,2, ZHENG Jinsong 1,2, XU Limei 1,2 and WANG Ding1*(1. Institute of Hydrobiology, chinese Academy of sciences, Wuhan 430072, china 2. graduate school of the chinese Academy of sciences, beijing 100039, china) Inst. of Hydrobiology Chinese Acad. of Sciences Wuhan 430072 China graduate Sch. Chinese Acad. of Sciences Beijing 100039 China

Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 23 individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

关键词： Yangtze finless porpoise isolated population reproductive behavior parentage

Effects of diatoms on reproduction and growth of marine copepods

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Progress in Natural Science:Materials International 2004年第10期14卷 1-7页

作者： ZHANG Guangtao 1,2 and SUN Song 1 (1.Key Laboratory of Marine Ecology&Environment sciences,Institute of Oceanology,chinese Academy of sciences,Qingdao266071,china 2.graduate school,chinese Academy of sciences,beijing100039,china) Lab. of Marine Ecology Inst. of Oceanology Chinese Acad. of Sci. Qingdao 266071 China graduate Sch. Chinese Acad. of Sci. Beijing 100039 China

Two hot spots in marine ecology,deleterious effects of diatoms and feeding selectivity of copepods,as well as new progress on these two issues achieved in the recent ten years,are *** two issues are considered correlated *** and their metabolites can induce deleterious effects on growth,reproduction and development of copepods,including increase of mortality and decrease of egg production,hatching and growth *** negative effects,resulting from either chemical toxin or nutritional defi-ciency,can be conquered in natural environments by diverse *** is therefore concluded that deleterious effects of diatoms observed in laboratory or during blooming period are only a special case that accommodation of feeding strategy of copepods is *** under-stand their feeding strategy in natural environments is a prerequisite to explaining the mechanisms of deleterious effects caused by diatoms,and makes it possible to re-evaluate the energy flow in marine ecosystems.

关键词： diatoms copepod feeding strategy reproduction deleterious effects

Trend of urban system structure under the restriction of water and land resources in Xinjiang

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chinese Science Bulletin 2006年第A1期51卷 179-188页

作者： ZHANG Xiaolei & LEIJun Xinjiang Institute of Ecology and Geography, chinese Academy of sciences, Urumqi 830011, china Institute of Geographical Science and Natural Resources Research, chinese Academy of sciences, beijing 100101, china graduate school, chinese Academy of sciences, beijing 100039, china Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi China Institute of Geographical Science and Natural Resources Research Chinese Academy of Sciences Beijing China graduate school Chinese Academy of Sciences Beijing China

根据在 Xinjiang 影响城市的开发和城市的系统结构的水和陆地资源上的研究，这篇论文分析利用地位和城市的水资源的缺乏。城市的水资源的消费和浪费在一个增加趋势，这被考虑。然而，在 Xinjiang 的大多数城市和镇在水资源的缺乏水资源... 详细信息

根据在 Xinjiang 影响城市的开发和城市的系统结构的水和陆地资源上的研究，这篇论文分析利用地位和城市的水资源的缺乏。城市的水资源的消费和浪费在一个增加趋势，这被考虑。然而，在 Xinjiang 的大多数城市和镇在水资源的缺乏水资源的废物是严肃的，特别在小镇上。megapolis 和中等城市的发展被有限陆地资源显然限制。尽管有扩展小城市和镇的相对大的空格，水和陆地资源的输出好处是低的。为了完成城市的系统的顺序的扩大，在 Xinjiang 组织，在都市化的快速的发展拿一个圆和三条带和节俭资源的策略的城市的空间发展模式被建议。

关键词：城镇体系结构水资源土地资源供水能力新疆

Wet-chemical synthesis and characteristics of Au nanoshell

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Science china Chemistry 2005年第5期48卷 431-435页

作者： LIU Zhongxin1,2, SONG Hongwei1, YU Lixin1,2, YANG Linmei1,2 & PAN Guohui1,2 1. Key Laboratory of Excited State Physics, Changchun Institute of Optics, Fine Mechanics and Physics, chinese Academy of sciences, Changchun 130033, china 2. graduate school of the chinese Academy of sciences, beijing 100039, china Key Laboratory of Excited State Physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun China graduate school of the chinese Academy of sciences Beijing China

Gold nanoshells were prepared by an easy wet-chemical method, with Ag nanoparticles used as the templates. Transmission electron microscopy (TEM) indicated that the shells were sphere in shape, the size was homogenous, being about 20 nm, and no hard ag-glomerates were observed. The plasma resonance absorption of gold was tuned from visible to near-infrared (NIR) with the increased volume of HAuCl4. The temperature grads (?T) of the gold nanoshell hydrosol under the exposure of an 808-nm optical fiber laser with different power den-sities were measured. The highest ?T was 30℃ (5 W/cm2, irradiation area was 2 cm2). This kind of gold nanoshell hydrosol is a promising material to be used in biomedicine such as photo-thermal cancer therapy, and its special photothermal convert property will photothermally trigger drug release.

关键词： wet-chemical synthesis, Au, nanoshell, NIR absorption, photothermal conversion.