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Cloning,Structural Organization and Chromosomal Mapping of Rat Costimulatory Molecule 4-1BBL

Acta Biochimica et Biophysica Sinica 2005年第10期37卷 694-701页

作者： Qiu-Ming DONG~1 Li-Jie MA~2 Guang-Bo ZHANG~1 Ya-Fang WU~3 Jia-Yao SHEN~1 Ying CHEN~1 Yong-Jing CHEN~1 Xiang-Ke PU~1 Sai-Yu HANG~1 Xue-Guang ZHANG~(1*) 1 Biotechnology institute,Soochow university,Suzhou 215007,china 2 genetics institute,fudan university,shanghai 200433,china 3 First Affiliated Hospital of Soochow university,Suzhou 215006,china Biotechnology institute Soochow UniversitySuzhou 215007 China genetics institute Fudan UniversityShanghai 200433 China First Affiliated Hospital of Soochow universitySuzhou 215006 China

4-1BBL (TNFSF9) is a member of the tumor necrosis factor (TNF) ligand superfamily,whichis expressed on some activated antigen presenting cells and B *** isolated a rat cDNA clone encodingthe rat homologue of the human 4-1BBL (GenBank accession ***259541).The deduced rat 4-IBBLprotein,consisting of 308 amino acids with a molecular weight of 33,469 Da,was a typical type Ⅱtransmembrane glycoprotein,the same as human and routine 4-IBBL.“SDAA” in the cytoplasmic domain ofrat 4-1BBL was deduced to act as the phosphorylation site for casein kinase Ⅰ(“SXXS” motif),which ispresent in the cytoplasmic domains of human and murine 4-1BBL,and all other TNF ligand family membersknown to utilize reverse *** two introns of 4-1BBL were also cloned (GenBank accession ***332409).Rat 4-IBBL is much more homologous with murine 4-1BBL than with human 4-1BBL,in bothnucleotide and amino acid *** 4-1BBL was expressed in all tested tissues:brain,lung,colon,liver,thymus,testicle,kidney,adrenal,stomach,spleen and *** chromosomal location of rat 4-1BBL wasfirst identified by bioinformatics,then by fluorescence in situ hybridization at 9q11(GenBank accessionUni Gene ***.46783).Rat,murine and human 4-1BBL genes are evolved from a common *** and characterization of the rat counterpart of human 4-1BBL will facilitate studies of the biologicalfunction of this molecule.

关键词： TNF superfamily rat 4-1BBL cloning mapping

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Cu(II) effect on the conformation of regenerated silk fibroin in dilute aqueous solution

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Science Bulletin 2005年第17期 45-49页

作者： ZONG Xiaohong1, ZHOU Ping1, SHAO Zhengzhong1, WANG Honghai2 & CHUNYU Lijuan2 1. The Key Laboratory of Molecular Engineering of Polymers, Ministry of education, Department of Macromolecular Science, fudan Univer-sity, shanghai 200433, china 2. institute of genetics, fudan university, shanghai 200433, china

Much attention has been paid to the natural mechanism of silkworm spinning due to the impressive me-chanical properties of the natural fibers. In this work, we studied the effect of Cu(II) ions on the secondary structure of Bombyx mori regenerated silk fibroin (SF) in dilute solution by circular dichroism (CD). The results indicate that a given amount of Cu(II) induces the SF conformational transition from random coil to β-sheet, however, further addition of Cu(II) is unfavorable for this conversion. Meanwhile, the conformational changes induced by Cu(II) follow a nuclea-tion-dependent aggregation mechanism, which is similar to that found in Prion protein (PrP) denaturation and Aβ-pep- tide aggregations, leading to the neurodegenerative disease. This work would help one understand further the natural spinning process of silkworm. Additionally, it would be sig-nificant for the study of the nervous system diseases, because silk fibroin, extracted in large amounts from Bombyx mori silkworm gland, could be a proper model to study PrP dena-turation and Aβ-peptide aggregations.

关键词： silk fibroin conformational transition Cu(II) ions cir-cular dichroism.

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大鼠4-1 BBLcDNA的克隆、分析及染色体定位

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苏州大学学报（医学版） 2005年第5期25卷 787-791,799页

作者：董秋明马力杰杭赛宇高磊张学光苏州大学生物技术研究所江苏苏州215007 复旦大学遗传所上海200433

目的克隆、分析并染色体定位肿瘤坏死因子配体超家族(Tumor Necrosis Factor Superfamily,TNFSF)成员4-1BBL分子。方法在对小鼠、人4-1BBL mRNA进行生物信息学分析的基础上,采用PCR扩增、T载体克隆测序等实验技术,并用软件对基因进行了... 详细信息

目的克隆、分析并染色体定位肿瘤坏死因子配体超家族(Tumor Necrosis Factor Superfamily,TNFSF)成员4-1BBL分子。方法在对小鼠、人4-1BBL mRNA进行生物信息学分析的基础上,采用PCR扩增、T载体克隆测序等实验技术,并用软件对基因进行了拼接、序列分析及同源性比较,同时通过荧光原位杂交(FISH)技术对该基因进行了染色体定位。结果首次获得了具有完整开放阅读框的大鼠cDNA,GenBank登录号为***259541,通过同源性比较分析,证明该基因与小鼠高度同源,编码区的核苷酸序列与小鼠、人的同源性分别为88%、37%。推导出的大鼠4-1BBL蛋白质由308个氨基酸分子组成,分子量为33469d,胞外区有三个潜在的N糖基化位点:AA138,AA160和AA292,是典型的Ⅱ型糖蛋白,与小鼠、人在氨基酸水平的同源性分别为83%、31%,其中胞质区为76%、32%,跨膜区为45%、40%,胞外区为87%、28%。该基因定位于大鼠染色体9q1(GenBank Uni-Gene登录号为***.46783)。结论大鼠4-1BBL胞内区的“SDAA”可能发挥着TNF家族中具有逆信号的其他成员的胞内区酪氨酸激酶Ⅰ磷酸化位点“SXXS”的作用。大鼠4-1BBLcDNA的成功克隆与定位为探讨4-1BBL在免疫共刺激中的作用机制以及TNF分子的进化研究打下了基础。

关键词： TNF超家族大鼠 4-1BBL cDNA 克隆染色体定位

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LDLR基因配体结合区多态性与丙型肝炎病毒易感性的相关研究

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苏州医学院学报 2001年第3期21卷 259-262页

作者：董秋明姜正文高锦声卢大儒苏州大学生命科学学院苏州215007 上海复旦大学遗传所上海200433

目的　探讨低密度脂蛋白受体 (LDLR)基因是否存在与丙型肝炎病毒 (HCV)感染有关的单核苷酸多态性 (SNP)。方法　利用PCR结合DNA自动测序对HCV感染者和正常人的LDLR基因的配体结合区进行分析。结果　没有发现与HCV感染相关的多态性位点... 详细信息

目的　探讨低密度脂蛋白受体 (LDLR)基因是否存在与丙型肝炎病毒 (HCV)感染有关的单核苷酸多态性 (SNP)。方法　利用PCR结合DNA自动测序对HCV感染者和正常人的LDLR基因的配体结合区进行分析。结果　没有发现与HCV感染相关的多态性位点。结论　LDLR基因的配体结合区与 (HCV)感染的易感性无关。

关键词： HCV LDLR 单核苷酸多态性配体结合区易感性丙型肝炎

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THE MODULI SPACE OF COMPLEX LAGRANGIAN SUBMANIFOLDS IN THE HYPER-KAEHLER MANIFOLD

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Chinese Annals of Mathematics,Series B 1999年第4期20卷 393-400页

作者： FU JIXIANG(institute of Mathematics, fudan university, shanghai 200433, china) institute of Mathematics Fudan University Shanghai 200433 China

The deformation of a compact complex Lagrangian submanifold in a hyper-Kaehler manifold and the moduli space are studied. It is proved that the moduli space Mc1 is a special Kaehler manifold, where special means that there is a real flat torsionfree symplectic connection satisfying dI = 0 (I is a complex structure of Mcl). Thus, following [4], one knows thatT*Mcl is a hyper-Kaehler manifold and then that Mcl is a complex Lagrangian submanifold in T* Mcl.

关键词： Moduli space Complex Lagrangian submanifold Hyper-Kaehler manifold Special Lagrangian submanifold Special Kaehler manifold Deformation

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THE OCCUPATION DENSITY FIELD FOR THE CATALYTIC SUPER-BROWNIAN MOTION

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Chinese Annals of Mathematics,Series B 1999年第4期20卷 447-454页

作者： HONG WENMING(institute of Mathematics, fudan university shanghai 200433, china) institute of Mathematics Fudan University Shanghai 200433 China

It is proved that the occupation time of the catalytic super-Brownian motion is absolutely continuous for d = 1, and the occupation density field is jointly continuous and jointly Holder continuous.

关键词： Branching rate functional Brownian collision local time Catalytic super-Brownian motion Occupation time Occupation density field

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GLOBAL CLASSICAL SOLUTIONS WITH SMALL INITIAL TOTAL VARIATION FOR QUASILINEAR HYPERBOLIC SYSTEMS

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Chinese Annals of Mathematics,Series B 2002年第3期23卷 335-348页

作者： YAN PING institute of Mathematics, fudan university, shanghai 200433, china institute of Mathematics Fudan University Shanghai 200433 China

By means of the continuous Glimm functional, a proof is given on the global existence of classical solutions to Cauchy problem for general first order quasilinear hyperbolic systems with small initial total variation.

关键词： Weak linear degeneracy Continuous Glimm functional Total variation Global classical solution Quasilinear hyperbolic system

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BLOW UP OF SOLUTIONS TO THE CAUCHY PROBLEM FOR NONLINEAR WAVE EQUATIONS

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Chinese Annals of Mathematics,Series B 2001年第3期22卷 275-280页

作者： ZHOU YI institute of Mathematics, fudan university, shanghai 200433, china institute of Mathematics Fudan University Shanghai 200433 China

The author proves blow up of solutions to the Cauchy problem of certain nonlinear equations and, also, estimates the time when the blow up occurs.

关键词： Cauchy problem Nonlinear wave equation Blow up

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ON A CONSTRUCTION OF INERTIALMANIFOLDS FOR A CLASS OF SECOND ORDER IN TIME EVOLUTION EQUATIONS

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Chinese Annals of Mathematics,Series B 1999年第3期20卷 271-278页

作者： WANG ZONGXIN(institute of Mathematics, fudan university, shanghai 200433, china.) institute of Mathematics Fudan University Shanghai 200433 China

Inertial manifolds for a class of second order in time dissipative equations are *** author also proves an asymptotic completeness property for the inertial manifolds and characterizes the inertial manifolds as the set of trajectories whose growth is at most of order O(e-μt ) for some p > 0. As applications, a nonlinear wave equation and a problem of nonlinear oscillations of a shallow shell are considered.

关键词： Dynamical systems Inertial manifolds Nonlinear evolution equations

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The neural mechanisms of pain-related affect and memory

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Progress in Natural Science:Materials International 2006年第4期16卷 338-345页

作者： ZHANG Yuqiu institute of Neurobiology, fudan university, shanghai 200433, china institute of Neurobiology Fudan University Shanghai 200433 China

The pain experience includes a sensory-discriminative and an emotional-affective components. The affective dimension refers to the unpleasantness or aversion of sensation. The great progress at the genetic, molecular, cellular, and systemic levels on the study of the sensory dimension of pain has been made over past four decades. However, "to consider only the sensory features of pain, and ignore its motivational and affective properties, is to look at only part of the problem and not even the most important part of that". A line of clinic observations indicate that the patients with chronic pain suffer from much more affective disturbance than pain itself. Obviously, physiological arousal and hypervigilance to pain cause negative affect, such as anxiety, anger, worry, aversion, even tendency of suicide, these negative affective states in turn enhance pain sensation. Today, more and more attention has been paid to the study on mechanisms underlying affective dimension of pain. In order to deepen and expand our understanding of the nature of pain, this review summarizes the main progress and recent findings from our laboratory regarding affective component of pain in neuroanatomy, neurophysiology, and cell biochemistry.

关键词： pain emotion medial pain system anterior cingnlate cortex formalin conditioned place avoidance cAMP responsive element binding protein extracellular signal-regulated kinase.

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