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Establishment and validation of stable functional human monoamine neurotransmitter transporter cell lines

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Establishment and validation of stable functional human mono...

Exsolution of ilmenite and Cr-Ti magnetite from olivine of garnet-wehrlite

中国药理学会第十一次全国学术会议

作者： AN Lei~(1,2),LI Jing~(1,2),LI Yun-feng~1,Li Jin~1,ZHANG You-zhi~1 (1.Institute of pharmacology and Toxicology,Academy of Military medical Science,Beijing 100850,china 2.College of Pharmacy,dalian medical university,dalian 116044,china)

OBJECTIVE To establish recombinant human monoamine neurotransmitter including serotonin,norepinephrine,dopamine transporters(hSERT,hNET,hDAT) stably transfected cell lines and validate their binding and uptake *** Lipofectamine transfection was applied to transfect hSERT,hNET and hDAT into parental cell human embryo kidney(HEK)293. The transfected cells were stably selected by 800 ng·μl G418 sulfate for about tow weeks,and the positive clones and their stabilities were identified by RT-PCR and Western blot *** binding and uptake function of hSERT,hNET and hDAT were identified by radioligand binding *** Results in RT-PCR and Western showed that HEK293-hSERT cell line was stably expressed hSERT RNA and protein in P1,P5,P10 and *** results were obtained in HEK293-hNET and HEK293-hDAT ***, the hSERT expressed in HEK293-hSERT cell demonstrated significantly specific binding activity to SSRIs[H]-citalopram. In the concenstration of 100 nmol·L[H]5-HT,the hSERT showed significantly 5-HT uptake function,and this action can be inhibited by 10μmol·L *** parental cell HEK293 did not possess these ***,HEK293-hNET and HEK293- hDAT showed specific binding activity to[H]nisoxetine(hNET) and[H]win35428(hDAT),and uptake function to[H]NE(50 nmol·L) and[H]DA(50 nmol·L) which also can be inhibited by Desiparmine(10μmol·L) and Nomifensine(10μmol·L).CONCLUSION The established HEK293-hSERT,HEK293-hNET and HEK293-hDAT cell lines which possess the function of endogenous hSERT,hNET and hDAT are stable functional monoamine neurotransmitter transporter cell *** account of the classic monoamine theory,these cell lines will be potent tools for screening and evaluation of antidepressant.

关键词： hSERT hNET hDAT HEK293 antidepressant

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Science china Earth Sciences 2005年第9期48卷 1368-1376页

作者： LIU Xiangwen1,2, JIN Zhenmin1,3, QU Jing2 & WANG Lu2 1. State Key Laboratory of Geological Processes and Mineral Resources, china university of Geosciences, Wuhan 430074, china 2. Graduate School of china university of Geosciences, Wuhan 430074, china 3. faculty of Earth Sciences, china university of Geosciences, Wuhan 430074, china 1. State Key Laboratory of Geological Processes and Mineral Resources China University of Geosciences 430074 Wuhan China 2. Graduate School of china university of Geosciences 430074 Wuhan China 3. faculty of Earth Sciences China University of Geosciences 430074 Wuhan China

Exsolution of rod-like ilmenite (Ilm) and Cr-Ti magnetite (Mt) have been found in olivine of garnet-wehrlite from the core of Chinese Continental Sciences Drilling (CCSD). Their composition, morphology, crystal structure and their topotaxies with host olivine have been studied in detail by the transmission electron microscopy (TEM) and electron probe microanaly- sis (EPMA) technique. It shows that rod-like Ilm exsolution reported in this paper has similar characteristic with that of Alpe Arami olivine, while Cr-Ti magnetite exsolution mentioned in this paper has large discrepancy with chromite exsolution in Alpe Arami olivine. These observations suggest that both of the exsolutions found in this paper should be solid solution phases in β-olivine at their first period, then experiencing decomposition of solid solution and therefore forming Ilm and Cr-Ti magnetite exsolution with the pressure decreasing. So, this garnet-wehrlite perhaps had been ever located in mantle transition zone with a minimum depth of 300 km.

关键词： garnet-wehrlite, ilmenite, magnetite, exsolution, CCSD.

Inhibitory effect of leflunomide on hepatic fibrosis induced by CCl4 in rats

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Acta Pharmacologica Sinica 2004年第7期25卷 69-74页

作者： Hong-wei YAO2,3, Jun LI3,4, Ji-qiang CHEN2, Shu-yun XU3 2 Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of china, School of Medicine, Zhejiang university, Hangzhou 310031 3Institute of Clinical pharmacology, Anhui medical university, Hefei 230032, china Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of china School of Medicine Zhejiang University Hangzhou 310031 Chin

AIM: To study the effect of leflunomide on CCl4-induced hepatic fibrosis in rats. METHODS: Hepatic fibrosis was induced by subcutaneous injection with 50 % CCl4 in Sprague-Dawley rats. The amount of CCl4 administered was 1 mg/kg. The alanine aminotransferase (ALT), aspartate aminotransferase (AST), nitric oxide (NO) levels in plasma and hydroxyproline (Hyp) contents in liver tissue were assayed by spectrophotometry. The hyaluronic acid (HA) and procollagen III (PC III) were assessed by radioimmunoassay. The transforming growth factor-β1 (TGF-β1) in serum was determined by ELISA. The nuclear factor-kappa B (NF-κB) in liver tissue was examined by immunohistochemistry. Liver samples collected after 12 weeks of CCl4 treatment were stained with hematoxy- lin and eosin. RESULTS: Leflunomide (1, 3, and 9 mg/kg) significantly decreased indices of liver and spleen, the serum transaminase (AST, ALT) activities, HA and PC III levels, and Hyp contents in liver tissue in rats of hepatic fibrosis. Histopathological examination showed leflunomide had inhibitory effect on fibrogenesis and formation of pseudolobulus. Furthermore, leflunomide significantly inhibited NF-κB expression in liver tissue, and reduced elevated serum TGF-β1 and NO levels in rats of hepatic fibrosis. CONCLUSION: Leflunomide showed inhibitory action on hepatic fibrosis induced by CCl4 in rats.

关键词： leflunomide hepatic fibrosis nuclear factor-kappa B transforming growth factor-beta nitric oxide

Effect of all trans-retinoic acid on the secretion of collagenase

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 588-590页

作者： Bing Li and Bo Tian dalian, china Department of General Surgery, First Affiliated Hospi- tal, dalian medical university, dalian 116011, china

BACKGROUND: Invasion and metastasis cause death o patients with liver cancer. Increased expression of matrix metalloproteinases ( MMPs ) is closely associated with tumor progression. Type collagen is the main structure protein of basilar membrane which is a natural barrier for inhibiting the metastasis of liver cancer cells. In this experi ment , we used all trans-retinoic acid (ATRA) to inhibi collagenase type in order to protect the type collagen and basilar membrane, further to suppress the metastasis o hepatocellular carcinomas. METHODS: By the use of cell culture and experimental animal models, the influence of all trans-retinoic acid (AT- RA) on the invasion of Hca-F liver cancer cells was studied in terms of adhesion capacity to artificial basilar membrane and production of collagenase type RESULT: ATRA could inhibit the adhesion capacity and collagenase secretion of Hca-F cells in a dose-dependent manner. CONCLUSION: ATRA can exert multiple effects on the in- vasion of Hca-F cells.

关键词： hepatocellular carcinoma retinoic acid matrix metalproteinase

The Mystery of Sleep:Molecular Mechanisms of Histaminergic Roles in Sleep-wake Regulation

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生物物理学报 2009年第S1期25卷 52-53页

作者： Zhi-Li Huang1,2,Wei-Min Qu2 1 State Key Laboratory of medical Neurobiology 2 Department of pharmacology, Shanghai medical College of Fudan university, Shanghai 200032, china.

The histaminergic neurons are confined to the tuberomammillary nucleus (TMN) of the posterior hypothalamus, and send their fibers to almost all regions of the brain. A

关键词： TMN

Theoretical analysis of polarization properties for tilted fiber Bragg gratings

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Science china(Information Sciences) 2010年第2期53卷 390-397页

作者： XU Ou 1 , LU ShaoHua 2 & JIAN ShuiSheng 2 1 faculty of Information Engineering, Guangdong university of Technology, Guangzhou 510006, china 2 Institute of Lightwave Technology, Beijing Jiaotong university, Beijing 100044, china 1. faculty of Information Engineering Guangdong University of Technology Guangzhou 510006 China2. Institute of Lightwave Technology Beijing Jiaotong University Beijing 100044 China

The polarization properties for tilted fiber Bragg gratings (TFBGs) are investigated theoretically based on coupled-mode theory and Mueller matrix method. The expression of wavelength-related polarizationdependent loss (PDL) for TFBGs with different tilt angles is derived and calculated. Simulation results are compared, and the results indicate that the polarization capability of TFBGs with 45° angle is stronger than other TFBGs with smaller angles. The degree of polarization for unpolarized light passing TFBGs is also simulated to further evaluate the polarization properties for various tilt angles. In addition, the relationship between physical parameters of a TFBG and its polarization capability is discussed.

关键词： coupled-mode theory degree of polarization (DOP) Mueller matrix method polarization dependent loss (PDL) tilted fiber Bragg gratings

转染人核糖核酸酶抑制因子基因对B16黑色瘤细胞及肿瘤转移的影响(英文)

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生物化学与生物物理进展 2004年第6期31卷 523-531页

作者：陈俊霞田余祥傅攀峰夏俊阎平崔秀云大连医科大学生物化学与分子生物学教研室大连116027 大连医科大学分析测试中心大连116027

人核糖核酸酶抑制因子 (humanribonucleaseinhibitor ,RI)是一种细胞质中分子质量为 5 0ku的酸性糖蛋白 .RI能抑制核糖核酸酶A (RNaseA)的活性 ,RNaseA与血管生成因子 (angiogenin ,Ang)的氨基酸有着高度保守的同源序列 .Ang是RNaseA超... 详细信息

人核糖核酸酶抑制因子 (humanribonucleaseinhibitor ,RI)是一种细胞质中分子质量为 5 0ku的酸性糖蛋白 .RI能抑制核糖核酸酶A (RNaseA)的活性 ,RNaseA与血管生成因子 (angiogenin ,Ang)的氨基酸有着高度保守的同源序列 .Ang是RNaseA超家族的一员 ,RI通过与RNaseA和Ang的紧密结合而抑制其活性 .血管生成及新血管的形成 ,是肿瘤发生和转移的必要条件 .所以抗血管生成将是一种很有希望的对抑制肿瘤生长和转移的有效方法 .实验显示RI能有效地抑制肿瘤诱导血管的生成 .RI由含有许多亮氨酸重复序列的多肽组成 .含有这样重复序列的 10 0多种蛋白质显示了广泛的功能 ,包括细胞周期调节 ,DNA修复 ,对细胞外基质相互作用以及抑制酶活性等 .RI被认为是胚胎发育 ,创伤愈合及肿瘤发生中新血管形成的一种调节因子 .RI定位于染色体的 11p15 5 ,与ras基因邻近 ,在肿瘤病人中经常存在染色体 11p15 5部位的变异和异常 .RI可能与细胞的生长和分化有关 ,因此 ,RI可能还具有尚未知的生物学作用 .为了进一步了解RI的潜在功能以及探讨RI与肿瘤侵润、转移的关系 ,将人的核糖核酸酶抑制因子基因的cDNA通过逆转录包装细胞PA317,并转染到B16小鼠黑色瘤细胞中 ,用转染空载体和未转染的B16细胞作为对照 .通过PCR ,RT PCR 。

关键词： B16细胞核糖核酸酶抑制因子血管生成肿瘤转移

Inhibitory effect of dendroaspis natriuretic peptide on spontaneous contraction in gastric antral circular smooth muscles of guinea pigs

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Acta Pharmacologica Sinica 2007年第11期28卷 1797-1802页

作者： Hui-shu GUO~(2,5),Zheng-xu CAI~3,Tai-hua WU~2,Jing XU~3,Yang QIU~2,Wen-xie XU~4~2 Centralab and ~3 Department of Neurology,The Fist Affiliated Hospital of dalian medical university,dalian 116011,china ~4 Department ofPhysiology Shanghai Jiao Tong university medical College,Shanghai 200240,china lab and Department of Neurology The Fist Affiliated Hospital of Dalian Medical University Dalian 116011 China. guohuishu1@***.

Aim:To determine whether the natriuretic peptide receptor (NPR) is present in thestomach of guinea pigs and to investigate the effect of dendroaspis natriureticpeptide (DNP) on the gastric motility of guinea pigs and its ***:The distribution of the NPR was analyzed by *** spon-taneous contraction of gastric antral circular muscles of guinea pigs was recordedby a 4-channel *** whole cell patch-clamp technique was intro-duced to record calcium-activated potassium currents in the gastric myocytesisolated by ***:The NPR existed in the gastric fundus,gastricbody,and gastric antrum of guinea pigs,and its density was largest in the *** inhibited spontaneous contraction and exhibited a *** DNP-induced inhibition was diminished by LY83583 (a guanylatecyclase inhibitor) and was potentiated by zaprinast (a cGMP-sensitivephosphoesterase inhibitor).The inhibitory effect of DNP on spontaneous con-traction was also inhibited by tetraethylammonium (a non-selective potassiumchannel blocker);10 nmol/L DNP increased the calcium-activated potassium cur-rents in the gastric circular myocytes of guinea ***:The NPR ismost common in the gastric antrum of guinea *** significantly inhibitsgastric motility in the gastric antrum of guinea *** inhibitory effect occursvia a cGMP-dependent pathway,and a calcium-activated potassium channel maybe also involved in the relaxation induced by DNP in gastric antral circular smoothmuscles.

关键词： dendroaspis natriuretic peptide motility natriuretic peptide receptor spontaneous contraction

The mechanism of bursting phenomena in Belousov-Zhabotinsky(BZ) chemical reaction with multiple time scales

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Science china(Technological Sciences) 2010年第3期53卷 748-760页

作者： BI QinSheng faculty of Science,Jiangsu university,Zhenjiang 212013,china 1. faculty of Science Jiangsu University Zhenjiang 212013 China

The dynamics of a typical Belousov-Zhabotinsky(BZ)reaction with multiple time scales is investigated in this *** forms of periodic bursting phenomena,and specially,three types of chaotic bursters with different structures can be obtained,which are in common with the behaviors observed in *** bifurcations connecting the quiescent state and the repetitive spikes are presented to account for the occurrence of the NKoscillations as well as the different forms of chaotic *** mechanism of the period-adding bifurcation sequences is explored to reveal why the length of the periods in the sequences does not change continuously with the continuous variation of the parameters.

关键词： Belousov-Zhabotinsky(BZ)reaction multiple time scales bursting phenomenon bifurcation mechanism