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In vitro and in vivo induction of bone formation based on adeno-associ-ated virus-mediated BMP-7 gene therapy using human adipose-derived mesenchymal stem cells

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Acta Pharmacologica Sinica 2007年第6期28卷 839-849页

作者： Yan KANG~2,Wei-ming LIAO~(2,5),Zhen-hua YUAN~4,Pu-yi SHENG~2,Long-juan ZHANG~3,Xiang-wei YUAN~2,Lei LEI~2~2 department of Orthopedics and ~3 Laboratory of General Surgery,the First Affiliated Hospital,{1.,guangzhou 51.080,China,~4 National Key Laboratory of Viral Genetic Engineering,National Institute for Viral Disease Control and Prevention,Chinese Center forDisease Control and Prevention,Beijing 1.0086,China departmentofOrthopedicstheFirstAffiliatedHospital SunYat-SenUniversityGuangzhou510080China LaboratoryofGeneralSurgery theFirstAffiliatedHospitalSunYat-SenUniversityGuangzhou510080China

Aim:To determine whether adeno-associated virus (AAV)-2-mediated,bone mor-phogenetic protein (BMP)-7-expressing human adipose-derived mesenchymal stemcells (ADMS) cells would induce bone formation in vitro and in ***:ADMS cells were harvested from patients undergoing selective suction-assistedlipectomy and transduced with AAV carrying the human BMP-7 ***-trans-duced cells and cells transduced with AAV serotype 2 carrying the enhancedgreen fluorescence protein gene served as *** cells were qualita-tively assessed for the production of BMP-7 and osteocalcin,and subjected toalkaline phosphatase (ALP) and Chinalizarin staining.A total of 2.5~ 1.6 cellsmixed with type Ⅰ collagen were implanted into the hind limb of severe combinedimmune-deficient (SCID) mice and subjected to a histological analysis 3 weekspost ***:Transfection of the ADMS cells achieved an effi-ciency of 99% at d *** with AAV2-BMP-7 induced the expression ofBMP-7 until d 56,which was markedly increased by d *** cells were positivelystained for *** production and matrix mineralization further con-firmed that these cells differentiated into osteoblasts and induced bone formationin vitro.A histological examination demonstrated that implantation of BMP-7-expressing ADMS cells could induce new bone formation in ***:The present in vitro and in vivo study demonstrated that human ADMS cellswould be a promising source of autologous mesenchymal stem cells for BMPgene therapy and tissue engineering.

关键词： adipose-derived mesenchymal stem ceils adeno-associated virus bone morphogenetic protein gene therapy ectopic bone formation

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Selective enrichment of hepatocytes from mouse embryonic stem cells with a culture system containing cholestatic serum

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Acta Pharmacologica Sinica 2007年第12期28卷 1931-1937页

作者： Jun MIN~(2,3),Chang-zhen SHANG~2,Ya-jin CHEN,Lei ZHANG,Lu LIU,Xiao-geng DENG,Mei YANG,Dong-ping CHEN,JunCAO,Er-wei SONG,Ji-sheng CHENdepartment of Hepatobiliary Surgery,The Second Affiliated Hospital of {1.,guangzhou 51.1.0,China department of Hepatobiliary Surgery The Second Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510120China

Aim:There is increasing evidence indicating that embryonic stem (ES) cells arecapable of differentiating into hepatocyte-like cells in ***,it is neces-sary to improve the differentiation efficiency so as to promote the ***,we report an efficient culture system to support hepatocytedifferentiation from ES cells by utilizing cholestatic ***:One weekafter the induction of E1. mouse ES cells into hepatocytes with sodium butyrate,cholestatic serum was added into the culture system at various concentrationsand hepatocyte-like cells were induced to *** morphological andphenotypic markers of hepatocytes were characterized using light microscopy,immunocytochemistry,and RT-PCR,*** function of glycogen stor-age of the differentiated cells was detected by Periodic acid-Schiff (PAS) reaction,and the ratio of hepatic differentiation was determined by counting the albuminand PAS-positive ***:In the presence of conditional selective mediumcontaining cholestatic serum,numerous epithelial cells resembling hepatocyteswere *** RT-PCR analysis showed that undifferentiated ES cells didnot express any hepatic-specific markers;however,in the presence of sodiumbutyrate and conditional selective medium containing cholestatic serum,hepaticdifferentiation markers were *** staining showed thatthose ES-derived hepatocytes were α-fetoprotein,albumin,and cytokeratin 1.positive,with the ability of storing *** determination of the hepaticdifferentiation ratio showed that the application of cholestatic serum efficientlyenriched ES-derived hepatocyte-like cells by inducing lineage differentiation andenhancing lineage ***:The conditional selective mediumcontaining cholestatic serum is optimal to selectively enrich hepatocyte-like cellsfrom mixed differentiated ES cells,which may provide a novel method to improvethe hepatic differentiation ratio of ES cells.

关键词： embryonic stem cells sodium butyrate hepatocyte cholestatic serum differentiation

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Griseofulvin enantiomers and bromine-containing griseofulvin derivatives with antifungal activity produced by the mangrove endophytic fungus Nigrospora ***1

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Marine Life science & {1. 2024年第1期6卷 102-114页

作者： Ge Zou Wencong Yang Tao Chen Zhaoming Liu Yan Chen Taobo Li Gulab Said Bing sun Bo Wang Zhigang She School of Chemistry Sun Yat-sen UniversityGuangzhou 510275China State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationInstitute of MicrobiologyGuangdong Academy of SciencesGuangzhou 510070China School of Pharmacy Anhui Medical UniversityHefei 230032China department of Chemistry Women University SwabiSwabi 23430Pakistan

Marine microorganisms have long been recognized as potential sources for drug *** was one of the first antifungal natural products and has been used as an antifungal agent for *** this study,1. new griseofulvin derivatives[(±)-1.2,(+)-3,(±)-4,1.-1.,and 1.-1.]and two new griseofulvin natural products(9 and 1.)together with six known analogues[(-)-3,5-8,and 1.]were isolated from the mangrove-derived fungus Nigrospora ***1.treated with 0.3%NaCl or 2%NaBr in rice solid *** 2D structures and absolute configurations were established by extensive spectroscopic analysis(1. and 2D NMR,HRESIMS),ECD spectra,computational calculation,DP4+analysis,and X-ray single-crystal *** 1.4 represent the first griseofulvin enantiomers with four absolute configurations(2S,6'S;2R,6'R;2S,6'R;2R,6'S),and compounds 9-1. represent the first successful production of brominated griseofulvin derivatives from fungi via the addition of NaBr to the culture *** the antifungal assays,compounds 6 and 9 demonstrated significant inhibitory activities against the fungi Colletotrichum truncatum,Microsporum gypseum,and Trichophyton mentagrophyte with inhibition zones varying between 28 and 41.mm(1.μg/disc).The structure-activity relationship(SAR)was analyzed,which showed that substituents at C-6,C-7,C-6'and the positions of the carbonyl and double bond of griseofulvin derivatives significantly affected the antifungal activity.

关键词： Mangrove endophytic fungus Nigrospora sp. Griseofulvin Antifungal activity

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3D-QSAR and Docking Analyses of c-Src Inhibitors

3D-QSAR and Docking Analyses of c-Src Inhibitors

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第十届全国计算(机)化学学术会议

作者： Wen Juan Wua,b,Rong Zhanga,Yong Shenb,Kang-Cheng Zhengb~* a department of Physical Chemistry,Guangdong Pharmaceutical {1.,guangzhou,51.006, b School of Chemistry and Chemical Engineering,sun yat-sen university,guangzhou,51.275,

Three-dimensional quantitative structure-activity relationship(3D-QSAR) and docking studies were performed on a series of cyanoquinoline derivatives inhibiting c-Src *** statistically significant model was established of 50 molecules,which were validated by a test set of 20 *** CoMFA model yielded a q=0.609,non cross-validated R of 0.891.F value of 1.6.267,and standard error of estimate=0.1.8 while the CoMSIA model yielded the best predictive model with a q=0.653,non cross-validated R of 0.843,F value of 1.2.735,and standard error of estimate=*** indicate that the 3D topology structure of active site of c-Src kinase from the docking analysis is in good agreement with 3D-QSAR model for this series of *** on the contour maps obtained from 3D-QSAR and docking analyses,some key structural factors of the compounds were revealed as follows:(1.The substituent R should be higher electronegativity and small steric volume groups.(2)Higher electronegativity on the first atom of substituent R is favorable to the activity.(3) Selecting substituent R’ to be a pyridyl group may increase the activity of compound,***,the interation information between target and ligand was pre{1.ted in *** results will be useful for design of novel and potent c-Src kinase inhibitors.

关键词： cyanoquinoline derivatives c-Src docking 3D-QSAR

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基于MPI常用海洋数值模式并行处理的研究

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实验技术与管理 2011年第5期 257-259页

作者：杨敏郭清顺何海涛中山大学网络与信息技术中心

分布式并行网格计算是目前各国科学家研究的一个热点课题。并行计算广泛应用于气象、环境、海洋、生物等领域,其中对海洋的研究是环境科学系、大气科学系的一个重要研究方向。依据消息传递接口(message passing interface,MPI)的特点,... 详细信息

分布式并行网格计算是目前各国科学家研究的一个热点课题。并行计算广泛应用于气象、环境、海洋、生物等领域,其中对海洋的研究是环境科学系、大气科学系的一个重要研究方向。依据消息传递接口(message passing interface,MPI)的特点,研究了基于MPI的并行环境下某种海洋数值模式并行计算的实现方法,其中包括MPI这种消息传递并行程序设计标准、FVCOM并行处理的原理和并行处理的部署实现。

关键词： MPI Parallel FVCOM

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Fabrication and field emission performance of arrays of vacuum microdiodes containing CuO nanowire emitters grown directly on glass without a catalyst

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science Bulletin 2011年第9期 908-913页

作者： SHAO PengRui, DENG ShaoZhi, CHEN Jun & XU NingSheng* State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Materials and technology, School of Physics and Engineering, {1., guangzhou 51.275, China

Arrays of vacuum microelectronic sources are fabricated on a glass substrate using cupric oxide (CuO) nanowire emitters. The arrays of electron sources possess a microdiode structure, which can effectively induce field emission and control the delivery of emitted electrons to the anode in a triode-type device operation. A technique for precisely growing CuO nanowires at the centre of microcavities in an array without using a catalyst and at temperatures as low as 400°C is presented. Such a simplified fabrication procedure results in improved field emission performance from the array compared with previous vacuum microelectronic devices. Typical prototype devices have turn-on gate voltages as low as 1.9 V to give emission current densities of 1. μA/cm2 at the anode. The ratio of anode current to cathode current reaches ~0.85, and the maximum change in emission current density per volt is 1.μA/cm2. Electron emission from the arrays is stable and reproducible under either pulsed or direct current fields. These characteristics indicate that microgate-controlled CuO nanowire emitters may find application in practical devices.

关键词： field emission CuO nanowires vacuum microdiodes arrays emitters

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Stable Surface Expression of a Gene for Helicobacter pylori Toxic Porin Protein with pBAD Expression System

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Journal of Huazhong university of {1. and technology(Medical {1.s) 2009年第4期29卷 435-438页

作者：彭志翔韦曦林正梅 department of Endodontics Guanghua College of StomatologySun Yat-Sen University

Helicobacterpylori （1.. pylori） infection causes peptic and duodenal diseases in humans. Among a 32-protein family of outer membrane proteins, a porin-like protein, HopE, has been a subject of note, mainly for its conservative nature among 1.. pylori, and for its potential as a vaccine candidate. To achieve stable surface expression of this host cell-toxic protein, hopE gene was introduced into pBAD expression system. After induction with arabinose, all 1. randomly-chosen E. coli LMG 1.4 colonies from 3 successive passages could express Hope protein, while only 1.from 5 E. coli colonies that contained lac operon-regulated plasmid encoding hopE gene could express HopE. Indirect immunofluorescence confirmed the expression of HopE on E. coli cell surface

关键词： hopE Helicobacter pylori porin pBAD expression system Escherichia coli lac op- eron-regulated plasmid

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同种异基因脐血移植小鼠模型的建立

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中国实验血液学杂志 2002年第6期10卷 564-567页

作者：黄绍良黄文革徐宏贵方建培魏菁陈凤英郭芬芬李树浓中山大学附属第二医院儿科广州510120 中山大学中山医学院实验动物中心广州510180 中山大学中山医学院病理生理教研室广州510180

探讨胎鼠及新生鼠外周血 (FNPB)移植特性 ,建立同种异基因脐血移植小鼠模型。采用体外集落培养和流式细胞术的方法 ,检测FNPB与骨髓 (BM )造血干 /祖细胞含量与特性。给致死量环磷酰胺预处理的BALB/c(H 2 d)小鼠经尾静脉输注C57BL/ 6(H ... 详细信息

探讨胎鼠及新生鼠外周血 (FNPB)移植特性 ,建立同种异基因脐血移植小鼠模型。采用体外集落培养和流式细胞术的方法 ,检测FNPB与骨髓 (BM )造血干 /祖细胞含量与特性。给致死量环磷酰胺预处理的BALB/c(H 2 d)小鼠经尾静脉输注C57BL/ 6(H 2 b)小鼠FNPB ,移植后动态观察受鼠存活状况、植入水平、造血与免疫功能恢复及移植物抗宿主病 (GVHD)发生情况。结果显示 :FNPB中早期红系祖细胞 (BFU E)和CD34+ 细胞含量与BM相近 ,粒单系祖细胞 (CFU GM) (1.4天 ) ,多向祖细胞 (CFU GEMM )及Sca 1.+ CD34+ 细胞亚群明显高于BM。FNPB移植能明显提高受鼠存活率 ,重建部分造血与免疫功能 ,未见明显GVHD表现 ,并在受体术后 2 1.骨髓检出供体源造血干细胞占 2 7.94%。结论 :FNPB富含造血干 /祖细胞。

关键词：同种异基因脐血移植小鼠建立动物模型

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Comparative Proteomics Analysis of Lungs Artificially Infected with H-PRRSV and N-PRRSV by Two-dimensional Fluorescence Difference Gel Electrophoresis

Comparative Proteomics Analysis of Lungs Artificially Infect...

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第四届中国畜牧科技论坛

作者： Xiao-shuqi~1.Wang-qiwei~1.Jia-jianyu~1.Cong-peiqing~1.Mo-delin~1.Yu-xiangchun~(1.2), Qin-limei~1.Li-anning~1.Niu-yuna~1.Zhu-kongju~1.Wang-xiaoying~1.Chen-yaosheng~(1.) (1.State Key Laboratory of Biocontrol,School of Life sciences,{1., guangzhou 51.006,China 2.Agronomy of Hainan university,Haikou 570228,China)

＜正＞Highly pathogenic porcine reproductive and respiratory syndrome（PRRS） was large-scalely outbreaked in China and Vietnam in 2006 and *** better understand host immune response to both highly virulent Chinese-type PRRSV（H-PRRSV） and normal North American-type PRRSV strains（N-PRRSV）,we firstly established an animal model infected with either H-PRRSV or *** differential proteomes of porcine lungs infected with H-PRRSV,N-PRRSV and uninfected negative control were analyzed at different time points using two-dimensional fluorescence difference gel electrophoresis（2D-DIGE） and mass spectrometry identification.45 differentially expressed proteins（DEPs） were *** proteins were mostly related to cytoskeleton,stress response and oxidation reduction or metabolism. In the protein interaction network constructed based on DEPs from lungs H-PRRSV infected, HSPA8,ARHGAP29 and NDUFS1.belonged to the most central proteins,whereas DDAH2,HSPB1.and FLNA corresponded to the most central proteins in those of N-PRRSV infected,suggesting differential host immune *** study is the first attempt to compare the complex picture of pulmonary protein expression during H-PRRSV and N-PRRSV infection under the in vivo environment using 2D-DIGE technology and bioinformatics tools,provides large scale valuable {1. for better understanding host immune response to these two PRRSV strains and potential molecular pathogenesis.

关键词： H-PRRSV N-PRRSV pig

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角膜基质诱导胚胎干细胞定向分化的初步实验研究

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眼科学报 1999年第4期15卷 195-198页

作者：王智崇葛坚陈家祺黄冰中山医科大学中山眼科中心广州510060 中山医科大学实验动物中心广州510060

目的:探索角膜基质接触诱导胚胎干细胞(embryonic stem cells,ES.细胞)定向分化的可能性。方法:分别在去上皮的新西兰白兔表层角膜缘基质上、晶状体上皮细胞饲养层上培养ES-D_3细胞,裸鼠皮下移植使其形成复层细胞,一段时间后,行扫描电... 详细信息

目的:探索角膜基质接触诱导胚胎干细胞(embryonic stem cells,ES.细胞)定向分化的可能性。方法:分别在去上皮的新西兰白兔表层角膜缘基质上、晶状体上皮细胞饲养层上培养ES-D_3细胞,裸鼠皮下移植使其形成复层细胞,一段时间后,行扫描电镜和光镜观察。结果:***细胞在新鲜表层角膜缘基质上增殖缓慢,2～3周形成较大细胞集落,光镜下细胞形态单一,体积较正常ES细胞大,电镜下细胞核已演变为细长形,移植到裸鼠皮下2周形成上皮样细胞复层,电镜下可见微绒毛,而角膜基质非上皮面的ES细胞仍保持其小细胞状态不变。2.晶状体上皮细胞与ES细胞共培养只能延缓其分化时间,不能诱导其定向分化。结论:表层角膜缘基质具有诱导ES细胞定向分化的潜能,体外培养条件下可能不发生晶状体诱导的第三级胚胎诱导。眼科学报1.99;1.:1.5-1.8。

关键词：胚胎干细胞角膜基质定向分化角膜移植

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