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Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia strain

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Journal of Environmental Sciences 2009年第2期21卷 218-222页

作者： WANG Wei,WEN Xianghua department of Environmental Science and Engineering,tsinghua university,beijing 100084,china. department of Environmental Science and Engineering Tsinghua University Beijing 100084 China

The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and expressed in Pichia pastoris X-33, a yeast. The cDNA of LiPH2 was generated from total RNA extracted from P chrysosporium by PCR with primers that do not contain a P. chrysosporium lignin peroxidase secretion signal. The gene was then successfully inserted into the expression vector pPICZα, and resulted in the recombinant vector pPICZα-lipH2. The transformation was conducted in two ways. One was using the wild Pichia pastoris as the recipients, which results in the recombinant P. pastoris with single or low lipH2 gene copy. The second was using P. pastoris and single or low lipH2 gene copy as the recipients, which results in the recombinant P. pastoris with multi-copies of lipH2 genes. This study firstly expressed the gene lipH2 in P. pastoris and achieved the successful expression of the lipH2 depending upon the generation of a recombinant strain that contained multiple copies. The lignin peroxidase activity reached a maximum of 15 U/L after 12 h induction.

关键词： heterologous expression lignin peroxidase Pichia pastoris

A SINGLE-PERIOD INVENTORY SYSTEM WITH A GENERAL S-SHAPED UTILITY AND EXPONENTIAL DEMAND

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Journal of Systems Science and Systems Engineering 2010年第2期19卷 227-236页

作者： Wei GENG1 Xiaobo ZHAO2 Deng GAO3 department of Industrial Engineering,tsinghua university,beijing 100084,china department of Industrial Engineering Tsinghua University Beijing China

We discuss a single-period inventory system with a general S-shaped utility function and exponential demand. It is shown that there exists a state-dependent order-up-to policy to be optimal. Behaviors of optimal polic... 详细信息

关键词： Inventory S-shaped utility function order-up-to policy

Investigation on extremal and critical characteristics of ignition time for H_2/O_2 combustion system and their applications

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Science china(Technological Sciences) 2009年第5期52卷 1161-1166页

作者： CHEN JianHua1,ZHANG HuiQiang2,LI ZhuoYi2 & ZHANG GuiTian1 1 School of Aerospace,North Western Polytechnical university,Xi’an 710072,china 2 department of Engineering Mechanics,tsinghua university,beijing 100084,china School of Aerospace North Western Polytechnical University Xi’an 710072 China department of Engineering Mechanics Tsinghua University Beijing 100084 China

The L,H and C curves in P-T phase are proposed to describe the minimal,maximal and critical char-acteristics of ignition time of H2/O2 combustion system,*** features of H2/O2(Air) com-bustion system,including explosion or not as well as the time delay to achieve its explosion status,can be well shown by explosion limits and these proposed *** curves can be described by 1.2k1=ks[Ms],(k11/k10+1)k1=ks[Ms],and 2k1=ks[Ms],respectively,which provide a physical explanation for these curves and give another way to establish *** on the contour of ignition time,the Z-type explosion limits can be explained by thermal explosion ***,the ignition distance of supersonic combustion is predicted according to the ignition time obtained in a Semenov system,which is very reasonable.

关键词： ignition time thermal explosion detailed reaction mechanism Semenov system supersonic combustion

LARGE DEFLECTION THEORY OF NANOBEAMS

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Acta Mechanica Solida Sinica 2010年第5期23卷 394-399页

作者： Dujuan Zeng Quanshui Zheng 1(department of Engineering Mechanics,tsinghua university,beijing 100084,china) [a] department of Engineering Mechanics Tsinghua University Beijing 100084 China

One-dimensional nanostructured materials are often used as beams in many applications such as ultrahigh-frequency resonators and ultrasensitive sensors. Compared with usual macroscopic beams, nanobeams have much higher surface/volume ratios so that their surface energies may play a significant role. Besides, they often bear large deflections due to their typically large slenderness ratios and larger elastic ranges. There is, however, lack of a theory that takes into account of both the above two features owned by nanobeams. In this paper, we present such a theory and give applied examples to show that surface energy and large deflection may individually or jointly have notable effects.

关键词： nanobeam surface energy large deflection

Molecular architecture and engineering of spider dragline silk protein

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Progress in Natural Science:Materials International 2005年第9期15卷 769-776页

作者： ZHANG Hengmu and LIU Jinyuan(department of Biological Sciences and Biotechnology, tsinghua university, beijing 100084, china) department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Spider dragline silk, which is produced in spider major ampullate gland, is a composite proteinacious fiber with highly repetitive Ala-Gly-rich domain. The unique combination of both high tensile strength and high elasticity makes spider dragline silk superior to almost any other natural or synthetic fibers. Cloning of the genes reveals that the silk is composed of at least two major proteins. Each protein component contains multiple repeats of modular structures that alternate between Ala-rich domains and Gly-rich domains. Molecular engineering not only opens a door to the production of spidroins but also provides a valuable experimental system to test and further establish the relationship between modular structures and mechanical properties. Here, based on our own studies, we review the latest progress of the modular structure and genetic engineering and outline the future prospects.

关键词： spider dragline silk protein modular structure molecular engineering.

Compactness in fuzzy logic

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Chinese Science Bulletin 1998年第14期43卷 1166-1171页

作者： YING Mingsheng department of Computer Science and Technology, tsinghua university, beijing 100084, china department of Computer Science and Technology Tsinghua University Beijing China

Compactness in Pavelka’s fuzzy logic for some compact lattices of truth values is shown, and the concept of gradual compactness is introduced to establish some corresponding results in a more general setting.

关键词： fuzzy logic compactness gradual compactness.

An atlas-based fuzzy connectedness method for automatic tissue classification in brain MRI

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Progress in Natural Science:Materials International 2006年第10期16卷 1106-1110页

作者： ZHOU Yongxin and BAI Jing (department of Biomedical Engineering, tsinghua university, beijing 100084, china) [a]department of Biomedical Engineering Tsinghua University Beijing China

A framework incorporating a subject-registered atlas into the fuzzy connectedness (FC) method is proposed for the automatic tissue classification of 3D images of brain MRI. The pre-labeled atlas is first registered onto the subject to provide an initial approximate segmentation. The initial segmentation is used to estimate the intensity histograms of gray matter and white matter. Based on the estimated intensity histograms, multiple seed voxels are assigned to each tissue automatically. The normalized intensity histograms are utilized in the FC method as the intensity probability density function (PDF) directly. Relative fuzzy connectedness technique is adopted in the final classification of gray matter and white matter. Experimental results based on the 20 data sets from IBSR are included, as well as comparisons of the performance of our method with that of other published methods. This method is fully automatic and operator-independent. Therefore, it is expected to find wide applications, such as 3D visualization, radiation therapy planning, and medical database construction.

关键词： fuzzy connectedness atlas-based segmentation brain tissue classification MR1.

Anomalous magnetotransport in LaMn_(1-x)Te_xO_3

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Science china(physics,Mechanics & Astronomy) 2009年第7期52卷 987-992页

作者： TAN GuoTai1,2, CHEN ZhengHao3 & ZHANG XiaoZhong4,5 1 Key Laboratory of Beam Technology & Materials Modification of the Ministry of Education, Institute of Low Energy Nuclear physics, beijing Normal university, beijing 100875, china 2 beijing Radiation Center, beijing 100875, china 3 Laboratory of Optical physics, Institute of physics, Chinese Academy of Sciences, beijing 100080, china 4 Key Laboratory of Advanced Materials of the Ministry of Education, department of Materials Science and Engineering, tsinghua university, beijing 100084, china 5 beijing National Center for Electron Microscopy, tsinghua university, beijing 100084, china 1. Key Laboratory of Beam Technology & Materials Modification of the Ministry of Education Institute of Low Energy Nuclear Physics Beijing Normal University Beijing 100875 China 2. beijing Radiation Center Beijing 100875 China 3. Laboratory of Optical physics Institute of Physics Chinese Academy of Sciences Beijing 100080 China 4. Key Laboratory of Advanced Materials of the Ministry of Education Department of Materials Science and Engineering Tsinghua University Beijing 100084 China 5. beijing National Center for Electron Microscopy Tsinghua University Beijing 100084 China

The LaMn1-xTexO3+δ (x=0.1, 0.2, 0.3) were synthesized using solid-state reaction method for the first time. X-ray photoemission spectrum (XPS) shows that in the samples the Te ions have a valence of Te4+, and Mn3+ ions are partly converted into Mn2+ and Mn4+ due to the excess oxygen and Te doping. The magnetotransport associated with Mn2+, Mn3+ and Mn4+ was investigated. The experimental results show that the samples are insulator at 0 T when the amount of Mn3+ is much larger or less than the sum of Mn2+ and Mn4+; by contrast, the samples display metal to insulator transition with increasing temperature when the amount of Mn3+ is close to the sum of Mn2+ and Mn4+. These anomalous magnetotransport behaviors were analyzed in the frame of the double-exchange (DE) mechanism.

关键词： colossal magnetoresistance La-Te-Mn-O magnetotransport

A novel extended Kalman filter for a class of nonlinear systems

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Progress in Natural Science:Materials International 2006年第9期16卷 912-918页

作者： DONG Zhe and YOU Zheng (department of Precision Instrument and Mechanology, tsinghua university, beijing 100084, china) [a]department of Precision Instrument and Mechanology Tsinghua University Beijing China

Estimation of the state variables of nonlinear systems is one of the fundamental and significant problems in control and signal processing. A new extended Kalman filtering approach for a class of nonlinear discrete-time systems in engineering is presented in this paper. In contrast to the celebrated extended Kalman filter (EKF), there is no linearization operation in the design procedure of the filter, and the parameters of the filter are obtained through minimizing a proper upper bound of the mean-square estimation error. Simulation results show that this filter can provide higher estimation precision than that provided by the EKF.

关键词： nonlinear systems state estimation Kalman filtering.