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Genomic research for important pathogenic bacteria in china

Science china(Life sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical microbiology,State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of microbiology and Epidemiology,Beijing 100071,china 2 department of medical microbiology and parasitology,institutes of medical sciences,shanghai jiao tong university school of medicine,shanghai 200025,china 3 State Key Laboratory for Molecular Virology and Genetic engineering,Institute of Pathogen Biology,Chinese Academy of medical sciences,Beijing 100176,china 4 State Key Laboratory for Infectious Disease Prevention and Control,Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,china 5 Beijing Genomics Institute at Shenzhen,Shenzhen 518083,china Laboratory of Analytical microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing China department of medical microbiology and parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai China State Key Laboratory for Molecular Virology and Genetic engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Beijing China State Key Laboratory for Infectious Disease Prevention and Control Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing China Beijing Genomics Institute at Shenzhen Shenzhen China

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

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Antibiotic Resistance of Probiotic Strains of Lactic Acid Bacteria Isolated from Marketed Foods and Drugs

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Biomedical and Environmental sciences 2009年第5期22卷 401-412页

作者： CHANG LIU, ZHUO-YANG ZHANG, KE DONG, JIAN-PING YUAN, AND XIAO-KUI GUO P1 P department of medical microbiology and parasitology, institutes of medical sciences, shanghai jiao tong university school of medicine, shanghai 200025, china department of medical microbiology and parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

Objective To identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics. Methods Antimicrobial susceptibility of 41 screened isolates was tested with disc diffusion and E-test methods after species-level identification. Resistant strains were selected and examined for the presence of resistance genes by PCR. Results Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, tetracycline, ampicillin, amoxicillin/clavulanic acid, cephalothin, and imipenem. In addition, isolates resistant to vancomycin, rifampicin, streptomycin, bacitracin, and erythromycin were detected, although the incidence of resistance to these antibiotics was relatively low. In contrast, most strains were resistant to ciprofloxacin, amikacin, trimethoprim/sulphamethoxazole, and gentamycin. The genes msrC, vanX, and dfrA were detected in strains of Enterococcus faecium, Lactobacillus plantarum, Streptococcus thermophilus, and Lactococcus lactis. Conclusion Antibiotic resistance is present in different species of probiotic strains, which poses a threat to food safety. Evaluation of the safety of lactic acid bacteria for human consumption should be guided by established criteria, guidelines and regulations.

关键词： Disc diffusion E-test MICs vanX msrC dfrA

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Granulocyte-macrophage colony-stimulating factor DNA prime-protein boost strategy to enhance efficacy of a recombinant pertussis DNA vac-cine

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Acta Pharmacologica Sinica 2006年第11期27卷 1487-1494页

作者： Qing-tian LI~(2,3,5)Yong-zhang ZHU~(2,3,5)Jia-you CHU~(2,6)Ke DONG~3 Ping HE~3 Chun-yan FENG~3 Bao-yu HU~3 Shu-min ZHANG~4 Xiao-kui GUO~(3,6)2 Institute of medical Biology,Peking Union medical College and Chinese Academy of medical sciences,Kunming 650118,china 3 department of medical microbiology and parasitology,shanghai jiao tong university school of medicine,shanghai 200025,china 4 Chinese National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,china Institute of medical Biology Peking Union Medical College and Chinese Academy of Medical Sciences Kunming China department of medical microbiology and parasitology Shanghai Jiao Tong University School of Medicine Shanghai China Chinese National Institute for the Control of Pharmaceutical and Biological Products Beijing China

Aim:To investigate a new strategy to enhance the efficacy of a recombinantpertussis DNA *** strategy is co-injection with cytokine plasmids asprime,and boosted with purified homologous ***:A recombinantpertussis DNA vaccine containing the pertussis toxin subunit 1(PTS 1),fragmentsof the filamentous hemagglutinin(FHA)gene and pertactin(PRN)gene encodingfilamentous hemagglutinin and pertactin were ***/c mice were im-munized with several DNA vaccines and antigen-specific antibodies anti-PTS1,anti-PRN,anti-FHA,cytokines interleukin(IL)-10,IL-4,IFN-γ,TNF-α,and spleno-cyte-proliferation assay were used to describe immune ***:Therecombinant DNA vaccine could elicit similar immune responses in mice as that ofseparate plasmids encoding the 3 fragments,*** immunized withDNA and boosted with the corresponding protein elicited more antibodies thanthose that received DNA as *** particular,when the mice were co-immu-nized with murine granulocyte-macrophage colony-stimulating factor plasmidsand boosted with proteins,all 4 cytokines and the 3 antigen-specific antibodieswere significantly increased compared to the pVAX1 ***-PTS1,anti-FHA,IL-4 and TNF-α elicited in the colony stimulating factor(CSF)prime-proteinboost group showed significant increase compared to all the other ***:This prime and boost strategy has proven to be very useful in im-proving the immunogenicity of DNA vaccines against pertussis.

关键词： granulocyte-macrophage colony-stimulating factor pertussis DNA vaccines

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Leukemia,an effective model for chemical biology and target therapy

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Acta Pharmacologica Sinica 2007年第9期28卷 1316-1324页

作者： Guo-qiang CHEN~(2,3,4),Li-shun WANG~2,Ying-li WU~2,Yun YU~2~2 department of Pathophysiology,Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,shanghai jiao tonguniversity school of medicine(SJTU-SM,formerly shanghai Second medical university),shanghai 200025,china,~3 Institute of HealthScience,SJTU-SM/shanghai institutes for Biological sciences,shanghai 200025,china department of Pathophysiology Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education Shanghai Jiao Tong University School of Medicine (SJTU-SM formerly Shanghai Second Medical University) Shanghai China Institute of Health Science SJTU-SM/Shanghai Institutes for Biological Sciences Shanghai China

The rapid rise of chemical biology aimed at studying signaling networks for basiccellular activities using specific,active small molecules as probes has greatlyaccelerated research on pathological mechanisms and target therapy of *** research is especially important for malignant tumors such as leukemia,aheterogeneous group of hematopoietic malignancies that occurs *** use of a chemical approach combined with genetic manipulation,great progresshas been achieved over the past few decades on the biological,molecular andcytogenetic aspects of leukemia,and in its diagnosis and *** particular,discoveries of the clinical effectiveness of all-trans retinoic acid and arsenictrioxide in the treatment of acute promyelocytic leukemia and the kinase inhibitorsImatinib and Dasatinib in the treatment of chronic myelogenous leukemia not onlymake target therapy of leukemia a reality,but also push mechanisms of leukemo-genesis and leukemic cell activities *** review will outline advances inchemical biology that help our understanding of the molecular mechanisms of celldifferentiation and apoptosis induction and target therapy of leukemia.

关键词： leukemia chemical biology target therapy retinoic acid arsenic trioxide differentia tion apoptosis

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Propofol attenuates oxidative stress-induced PC12 cell injury via p38 MAP kinase dependent pathway

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Acta Pharmacologica Sinica 2007年第8期28卷 1123-1128页

作者： Xing-jun WU~2 Yong-jun ZHENG~3 Yong-yao CUI~2 Liang ZHU~2 Yang LU~(2,4) Hong-zhuan CHEN~(2,4) ~2department of Pharmacy shanghai jiao tong university school of medicine,shanghai 200025,china ~3department of Anesthesiology,Renji Hospital,shanghai jiao tong university school of medicine,shanghai 200001,china department of Pharmacy Shanghai Jiao Tong University School of Medicine Shanghai China department of Anesthesiology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai China

Aim:To investigate the neuroprotective effect of propofol and its intracellularmechanism on neurons in ***:Cell viability was determined with 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide *** celldeath was determined by Hoechst 33258 staining and a fluorescence-activatedcell *** caspase-3 activity was measured by fluorometric ***-activated protein（MAP）kinase phosphorylation was detected with ***:The pretreatment of rat pheochromocytoma cell line PC 12 withpropofol（1-10μmol/L）resulted in a significant recovery from hydrogen peroxide（H2O2）-induced cell death and the inhibition of H2O2induced caspase-3 activationand PC12 cell *** inhibited the H2O2-induced p38 MAP kinase,but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1 and ***:Propofol might attenuate H2O2-induced PC12 cell deaththrough the inhibition of signaling pathways mediated by the p38 MAP kinase.

关键词： propofol oxidative stress PC12 cells apoptosis p38 MAP kinase

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Expression and regulation of versican in neural precursor cells and their lineages

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Acta Pharmacologica Sinica 2007年第10期28卷 1519-1530页

作者： Wen-li GU~(2,3),Sai-li FU~2,Yan-xia WANG~2,Ying LI~2,Xiao-fei WANG~2,Xiao-ming XU~(2,4),Pei-hua LU~(2,5)~2 department of Neurobiology,shanghai jiao tong university school of medicine,shanghai 200025,china ~3 department of ClinicalLaboratory,Ninth People's Hospital of shanghai,shanghai jiao tong university,shanghai 200011,china ~4 department of Neurological Surgery,university of Louisville school of medicine,Louisville,Kentucky 40292,USA department of Neurobiology Shanghai Jiao Tong University School of Medicine Shanghai China department of Clinical Laboratory Ninth People's Hospital of Shanghai Shanghai Jiao Tong University Shanghai China department of Neurological Surgery University of Louisville School of Medicine Louisville USA

Aim:To have a better understanding of the expression and regulation of versicanisoforms in neural precursor cells (NPC) and ***:By immunocytochemistry,RT-PCR,and real-time PCR,we examined the tem-poral expression of versican in NPC isolated from embryonic d 16 rats as wellas in oligodendrocyte (OL) lineage cells induced to differentiate from NPC,which mimicked the oligodendrogliogenesis in ***:We found thatversican was constitutively expressed in NPC and their lineage cells,includingneurons,astrocytes,and *** addition,2 versican isoforms,V1/V0 and V2,were found to express at low levels in NPC,but at significantly higher levels inOL lineage *** peak expression of versican V2 was found at the oligo-dendrocyte precursor cell ***,the treatment of 2 pro-inflam-matory cytokines,TNF-α and IFN-γ,enhanced the transcription of versican V2in NPC in a dose-dependent manner,but showed no effect on V1/V0 ***:Taken together,our results demonstrate that versican,particularlythe inhibitory V2 isoform,is increasingly expressed in OL lineage cells inducedto differentiate from *** increase in versican V2 expression after cytokinestimulation implies the interplay between the injury-induced upregulation ofinflammatory cytokines and chondroitin sulfate proteoglycan-mediated inhibi-tion of axonal regeneration after central nervous system injury.

关键词： chondroitin sulfate proteoglycans neural precursor cells oligodendrocyte precursor cells oligodendrocytes pro-inflammatory cytokines versican

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Current advances in the application of proteomics in apoptosis research

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Science china(Life sciences) 2011年第3期54卷 209-219页

作者： WANG LiShun & CHEN GuoQiang department of Pathophysiology,Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education of china E-institutes of shanghai Municipal Education Commission Chemical Biology Division,shanghai jiao tong university school of medicine,shanghai 200025,china department of Pathophysiology Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education of China E-institutes of shanghai Municipal Education Commission Chemical Biology Division Shanghai Jiao Tong University School of Medicine Shanghai China

Apoptosis,or programmed cell death,is a complex,genetically-determined process involved in the development and maintenance of homeostasis in multicellular *** of apoptosis has been implicated in a number of diseases,including cancer and autoimmune ***,the investigation of apoptotic regulation has evoked considerable *** apoptotic proteins have been shown to be post-translationally modulated,such as by protein cleavage,translocation,protein-protein interaction,and various post-translational modifications,which fall precisely within the range of proteomic ***,contemporary proteomic technologies have achieved significant advances and have accelerated research in functional and chemical proteomics,which have been applied to the field of apoptosis research and have the potential to be a driving force for the *** review highlights some of the major achievements in the application of proteomics in apoptosis research and discusses new directions and challenges for the near future.

关键词： apoptosis proteomics small compound oncogene tumor suppressor

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Laparoscopic low anterior resection for rectal carcinoma:Complications and management in 132 consecutive patients

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世界胃肠病学杂志：英文版 2010年第36期16卷 4605-4610页

作者： Qian-Lin Zhu,Bo Feng,Ai-Guo Lu,Ming-Liang Wang,Wei-Guo Hu,Jian-Wen Li,Zhi-Hai Mao,Min-Hua Zheng,department of General Surgery,shanghai Ruijin Hospital,shanghai jiao tong university school of medicine,shanghai 200025,china shanghai Minimally Invasive Surgery Center,shanghai 200025,china department of General Surgery Shanghai Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China.

AIM:To analyze the clinical manifestations and risk fac-tors of complications in laparoscopic low anterior resection(LAR)for rectal cancer ***:A series of 132 consecutive patients who received laparoscopic LAR for rectal cancer in our center were *** etiology,diagnosis,treatment and prevention of rectal cancer were studied among the patients with surgery-related complications using both univariate and multivariate regression ***:No conversion to open surgery was observed and 5 cases converted to hand-assisted laparoscopic *** overall morbidity rate was 20.5%.Complications occurred during the operation in 7 patients(5.3%),within 30 postoperative days in 24 patients(18.2%),and within 3 mo in 2 patients(1.5%).The most significant complications were anastomotic leak-age(9.1%)and anastomotic hemorrhage(5.3%).Sizeand location of tumor,pathological staging and preoperative nutrition were significant factors associated with LAR complications,while gender,age and pathological type showed no *** logistics regression showed that the size and location of tumor,and pathological staging were independent factors of laparoscopic *** the complications were treated during their onset of clinical manifestations by interventional or conservative ***:Anastomotic leakage is a major com-plication in laparoscopic *** complications may be associated with tumor size and site,and pathological *** therapies are of value in the management of laparoscopic LAR complications.

关键词： Laparoscopy Low anterior resection Complication Rectal cancer Logistic regression analysis

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Prevention of pathological change and cognitive degeneration of Tg2576 mice by inoculating Aβ_(1-15) vaccine

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Science china(Life sciences) 2008年第8期51卷 743-750页

作者： HU JinJia1, LI GuoYing2, WANG HuaQiao3, LIN Xian3 & YAO ZhiBin3 1 department of Anatomy, medical College of shanghai jiao tong university, shanghai 200025, china 2 department of Anatomy, Preclinical College, Guangdong university of Pharmacy, Guangzhou 510006, china 3 department of Anatomy, Zhongshan school of medicine, Sun Yat-sen university, Guangzhou 510080, china department of Anatomy Medical College of Shanghai Jiao Tong University Shanghai China department of Anatomy Preclinical College Guangdong University of Pharmacy Guangzhou China department of Anatomy Zhongshan School of Medicine Sun Yat-sen University Guangzhou China

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2576 mice by inoculating the subunit fragment of Aβ ***-two Tg2576 mice were randomly divided into four groups, each having eight mice:Group I, the control group, inoculated with adjuvants;Group II, the Aβ42 group, inoculated with Aβ42 vaccine;Group Ⅲ, the Aβ1―15 group, inoculated with Aβ1―15 vaccine;and Group IV, the Aβ36―42 group, inoculated with Aβ36―42 *** titer of the serum anti-body against Aβ42(Group II) was significantly higher than that of the control group(Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated *** water maze test showed that the Aβ42 group, Aβ1―15 group and Aβ36―42 group were obviously im-proved compared with the control *** cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control *** the Aβ42 group, IL2 and IFN-γ were relatively low and IL4 and IL10 were relatively *** contrast, IL4 and IL10 were much higher in the Aβ1―15 group and IL2 and IFN-γ were much higher in the Aβ36―42 *** immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the con-trol group, no senile plaque in the brain of the Aβ1―15 group and Aβ42 group mice, and a small number of senile plaques in the brain of the Aβ36―42 group *** results suggest that the subunit fragment of Aβ1―15 vaccine could prevent not only cognitive and behavioral degeneration but also Aβ deposition and formation of senile plaques in Tg2576 mice.

关键词： Alzheimer’s disease β-amyloid peptide vaccine Tg2576 mice senile plaque

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Germline mutation analysis of hPMS2 gene in Chinese families with hereditary nonpolyposis colorectal cancer

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World Journal of Gastroenterology 2010年第30期16卷 3847-3852页

作者： Xia Sheng, Xiao-Yan Zhou, Xiang Du, Tai-Ming Zhang, WeiQi Sheng, Da-Ren Shi, department of Pathology, shanghai Cancer Center, Fudan university, shanghai 200032, china department of Oncology, shanghai medical College, Fudan university, shanghai 200032, china Heng-Hua Zhou, department of Pathology, shanghai Ninth People’s Hospital Affi liated to shanghai jiao tong university school of medicine, shanghai 200011, china San-Jun Cai, department of Abdominal Surgery, Cancer Center, Fudan university, shanghai 200032, china department of Pathology Shanghai Cancer Center Fudan University Shanghai 200032 China.

AIM: To study the germline mutation of hPMS2 gene in 26 unrelated Chinese hereditary nonpolyposis colorectal cancer (HNPCC) probands and to fulfill the screening strategy for HNPCC in Chinese. METHODS: Genomic DNA was extracted from the peripheral blood. To avoid the interference of pseudogene in detection of the remaining 11 exons (exon 1-5, 9, 11-15), long-range polymerase chain reaction (PCR) was conducted to amplify the complete coding region of hPMS2 gene firstly. Then 1/8 of the PCR productswere used as template to amplify the individual exon respectively and DNA sequencing was done. Direct DNA sequencing of the conventional PCR products of exon 6, 7, 8 and 10 of hPMS2 gene was performed. The same analysis was made in 130 healthy persons without family histories of HNPCC to further investigate the pathological effects of the detected missense mutation. RESULTS: One HNPCC proband fulf illed Bethesda guidelines and was found to carry the germline mutation of hPMS2 gene, which has not been reported in Chinese HNPCC families. It was a missense mutation at c.1532C>T of exon 11. It was detected in three controls as well with an occurrence rate of 2.3% (3/130). Since it could not be found in the PMS2-single nucleotide polymorphism (SNP) database, this missense mutation is a new SNP unreported up to date. Meanwhile, 260 reported SNPs of hPMS2 gene were detected in the 26 HNPCC probands. The 2nd and 5th exons were probably the hot SNP regions of hPMS2 gene in Chinese HNPCC families involving 53.1% of all reported SNP. CONCLUSION: The germline mutation of hPMS2 gene may be rare in Chinese HNPCC families. The 2nd and 5th exons are hot SNP regions of hPMS2 gene.

关键词： Hereditary nonpolyposis colorectal cancer hPMS2 Missense mutation Single nucleotide polymorphism Colorectal cancer

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