检索结果-南通市图书馆

华中科技大学学报（医学版） 2009年第3期38卷 281-285页

作者：沈建英徐桂香左斌甘辉肖娟娟张敏海汪薇曦李宏莲华中科技大学同济医学院基础医学院解剖学系组织胚胎学教研室武汉430030

目的探讨磷酸酯酶活性降低致tau过度磷酸化与细胞凋亡的关系。方法稳定表达人tau cDNA的鼠成神经瘤细胞(N2a/tau)分3组,对照组不处理;十字孢碱(staurosporine,STP,凋亡诱导剂)处理6h为STP组;用磷酸酯酶PP-2A的抑制剂冈田酸(okadaic acid... 详细信息

目的探讨磷酸酯酶活性降低致tau过度磷酸化与细胞凋亡的关系。方法稳定表达人tau cDNA的鼠成神经瘤细胞(N2a/tau)分3组,对照组不处理;十字孢碱(staurosporine,STP,凋亡诱导剂)处理6h为STP组;用磷酸酯酶PP-2A的抑制剂冈田酸(okadaic acid,OA)处理4h,再用STP处理6h为OA+STP组。流式细胞仪检测凋亡率,免疫印迹检测Ser396位点磷酸化tau(Ser396 p-tau)和总tau的表达,免疫荧光双标检测Ser396 p-tau的阳性产物与活化的半胱氨酸蛋白酶(Cleaved Caspase-3)阳性产物的共定位关系。结果①凋亡率:对照组为(4.37±1.47)%,STP组为(19.84±3.25)%,OA+STP组为(12.12±2.46)%;②免疫印迹:OA+STP组Ser396 p-tau的表达明显高于另两组,总tau表达组间无差异;③免疫荧光双标:进一步印证免疫印迹结果,且3组均显示活化的Caspase-3和Ser396 p-tau阳性产物大多不共定位于相同细胞。结论PP-2A活性降低所致tau Ser396位点的过度磷酸化可抑制细胞凋亡,结合前期的实验进一步明确特殊位点过度磷酸化的tau可抑制细胞进入快速的凋亡程序。

关键词：阿尔茨海默病 tau蛋白磷酸酯酶PP-2A 细胞凋亡

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expert Consensus on Pathological Diagnosis of Intrahepatic Cholangiocarcinoma(2022 version)

引用

Journal of Clinical and Translational Hepatology 2023年第7期11卷 1553-1564页

作者： Han Wang Jun Chen Xin Zhang Xia Sheng Xiao-Yan Chang Jie Chen Min-Shan Chen Hui Dong Guang-Jie Duan He-Ping Hu Zhi-Yong Huang Wei-Dong Jia Xiao-Qing Jiang Dong Kuang Shan-Shan Li Zeng-Shan Li Chang-Li Lu Shu-Kui Qin Xue-Shan Qiu Li-Juan Qu Chun-Kui Shao Feng Shen Guo-Ming Shi Su-Sheng Shi Yu-Jun Shi Hui-Chuan Sun Xiao-Dong Teng Bin Wang Zhan-Bo Wang Tian-Fu Wen Jia-Mei Yang Qiao-Qiao Yang Sheng-Long Ye Hong-Fang Yin Zhen-Gang Yuan Jing-Ping Yun Feng-Lin Zang Hong-Qi Zhang Li-Hong Zhang Jing-Min Zhao Jian Zhou Wei-Xun Zhou Jia Fan Xiao-Ping Chen Wan Yee Lau Yuan Ji Wen-Ming Cong Chinese Society of Liver Cancer of Chinese Anti-Cancer Association Digestive Disease Group of Chinese Society of Pathology,Chinese medical Association Chinese Society of Pathology of Chinese Anti-Cancer Association Hepatic Surgery Group of Chinese Society of Surgery,Chinese medical Association Biliary Tract Tumor Committee of china Anti-Cancer Association Chinese Society of Clinical Oncology Idepartment of Pathology Eastern Hepatobilary Surgery HospitalNaval Medical UniversityShanghaiChina department of Pathology the Affiliated Drum Tower HospitalNanjing University Medical SchoolNanjingJiangsuChina department of Pathology Zhongshan HospitalFudan UniversityShanghaiChina department of Pathology Minhang HospitalFudan Uni-versityShanghaiChina department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sci-ences and Peking Union Medical CollegeBeijingChina department of Liver Surgery Sun Yat-Sen University Cancer CenterGuangzhouGuangdongChina department of Pathology The First Affiliated HospitalArmy Medical UniversityChongqingChina department of Hepatobiliary Medicine Eastern Hepatobiliary Surgery HospitalNaval Medical UniversityShanghaiChina Hepatic Surgery center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWu-hanHubeiChina department of General Surgery The First Affliated Hospital of USTCDivision of Life Science and Medi-cineUniversity of Science and Technology of ChinaHefeiAnhuiChina department of Biliary Surgery I Eastern Hepatobil-iary Surgery HospitalNaval Medical UniversityShanghaiChina department of Pathology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiChina department of Pathology The First Afiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenanChina department of Pathology Xijing Hospi-talAir Force Medical UniversityXi'anShaanxiChina department of Pathology West China HospitalSichuan UniversityChengduSichuanChina Cancer center of Jinling Hospital Nanjing University of Chinese MedicineNanjingJiangsuChina department of Pathology The First Affiliated HospitalChina Medical UniversityShenyangLiaoningChina department of Pathology The 900 Hospital of the Chinese People's Liberation Army Joint Logistics TeamFuzhouFujianChina department of Pathology The Third Affiliated HospitalSun Yat-Sen UniversityGuangz

Intrahepatic cholangiocarcinoma(iCCA)can originate from the large bile duct group(segment bile ducts and area bile ducts),small bile duct group(septal bile ducts and interlobular bile ducts),and terminal bile duct group(bile ductules and canals of Hering)of the intrahepatic biliary tree,which can be histopathological corresponding to large duct type iCCA,small duct type iCCA and iCCA with ductal plate malformation pattern,and cholangiolocarcinoma,*** challenge in pathological diagnosis of above subtypes of iCCA falls in the distinction of cellular morphologies,tissue structures,growth patterns,invasive behaviors,immunophenotypes,molecular mutations,and surgical *** these reasons,this expert consensus provides nine recommendations as a reference for standardizing and refining the diagnosis of pathological subtypes of iCCA,mainly based on the 5th edition of the World health Organization Classification of Tumours of the Digestive System.

关键词： Intrahepatic cholangiocarcinoma Pathology Histological type Immunohistochemistry Molecular variation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：