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The reintroduction and population development of Père David’s deer in Shishou,Hubei

第七届全国野生动物生态与资源保护学术研讨会

作者： Zhang Mingming~1,Liu Zhensheng~(1,2),Teng Liwei~(1,2*) (1 college of wildlife resources,northeast forestry university,harbin 150040,China 2 Key Laboratory of Conservation Biology,State forestry Administration,harbin 150040,China.)

The red deer Cervus elaphus alxaicus is an endemic species in China,distributed in the central region of Helan *** habitat selection of *** was studied from December 2007 to December *** on the feeding and bedding habitats were mainly conducted by observation of fresh signs of their presence such as bedding,foraging signs and feces,assisted by direct observation of their activities. We compared the habitats used by red deer(observed plots)with those that are available(expected plots, assuming no differential selection by deer)to study the habitat selection.A total of 602 plots used by red deer(observed plots)along the 32 transects were recorded and compared among the four vegetation type *** provide comparison plots for the analysis of habitat selection,we surveyed an additional 617 plots by systematically placing transects in areas with no obvious evidence of red deer *** comparison plots were estimated by the proportion of used plots found in each vegetation type zone. To assess differences of the 18 factors between used and comparison plots in different seasons,a chi-square goodness-of-fit test within classified categories for each variable was *** data of non-numeric ecological factors were examined with chi-square *** of the remaining numeric ecological variables were initially analyzed with one-sample Kolmogorov-Smirnov tests to determine if they were normally *** normally distributed data were analyzed with independent-samples t-tests,while the non-normally distributed data were analyzed with Kruskal-Wallis H *** data of the 18 variables were also subjected to principal component analysis(PCA)to identify the most important habitat variables used by red deer during the year. The result showed that habitat selection widely varied among different *** deer have a seasonal movement among habitats of high elevations and low elevations because of changes in forage availability,special alp

关键词： habitat selection seasonal movement Cervus elaphus alxaicus the Helan Mountains

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The reintroduction and population development of Père David...

野生动物生态与资源保护第三届全国学术研讨会

作者： YANG Daode~(1,2,3) MA Jiangzhang~2 JIANG Zhigang~3 LI Pengfei~4 (1 Institute of wildlife Conservation,Central South university of forestry & Technology,Changsha 410004 2 college of wildlife resources,northeast forestry university,harbin 150040 3 Institute of Zoology,Chinese Academy of Science,Beijing 100080 4 Bureau of Hubei Shishou Milu National Nature Reserve,Shishou County 434400)

＜正＞The final goal of the species reintroduction is to establish self-sustained wild *** reintroduction of Père David’s deer is the first large-size species reintroduction project in *** order to protect the Père David’s Deer,From July 2000 to April 2006,we studied the reintroduction and population development of the Père David’s deer population in the Shishou National Nature *** results were as follows:

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施氏鲟卵巢发育的组织学观察

水产学报 2004年第5期28卷 487-492页

作者：曲秋芝孙大江王丙乾马建中国水产科学研究院黑龙江水产研究所东北林业大学野生动物资源管理学院黑龙江哈尔滨　150070 黑龙江哈尔滨　150040

对人工养殖施氏鲟卵巢发育的不同时期(1～8龄)进行了组织学观察,结果表明:1龄施氏鲟处于性分化早期,卵巢中卵原细胞形成增殖团;2～3龄鱼卵巢为I期,第1时相的初级卵母细胞直径10～60μm,核大,位于细胞中央,染色质均匀分布;4～5龄卵巢为Ⅱ... 详细信息

对人工养殖施氏鲟卵巢发育的不同时期(1～8龄)进行了组织学观察,结果表明:1龄施氏鲟处于性分化早期,卵巢中卵原细胞形成增殖团;2～3龄鱼卵巢为I期,第1时相的初级卵母细胞直径10～60μm,核大,位于细胞中央,染色质均匀分布;4～5龄卵巢为Ⅱ期,第2时相小生长期的初级卵母细胞直径60～200μm,核内含有7～26个核仁;6～7龄卵巢Ⅲ期,第3时相大生长期的初级卵母细胞直径200～1600μm,卵母细胞内出现脂肪滴、卵黄颗粒和色素颗粒。细胞膜有完整的3层结构。8龄卵巢Ⅳ期,第4时相晚期初级卵母细胞直径1600～3300μm,细胞核膜消失,核仁溶解,核由中心向动物极受精孔移动;Ⅴ期卵巢的第5时相生殖细胞为成熟的卵细胞,直径3400～3750μm,从滤泡膜中释放到体腔内;Ⅵ期为产卵后的卵巢,以第2时相的卵母细胞为主。根据研究结果推测,施氏鲟卵巢再次成熟的周期为3～4年。

关键词：施氏鲟卵巢发育组织学

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史氏鲟消化系统形态学与组织学观察

水产学报 2003年第1期27卷 1-6页

作者：曲秋芝华育平曾朝辉齐艳萍王永林王兵中国水产科学研究院黑龙江水产研究所东北林业大学野生动物资源学院东北林业大学野生动物资源学院黑龙江哈尔滨　150070 黑龙江哈尔滨　150040

通过对史氏鲟的活体解剖和对消化系统各组织的观察,结果表明史氏鲟具有胃、幽门盲囊和瓣肠,胰脏形成独立的器官,在肝门部有部分弥散于肝脏组织中。与同属的中华鲟比较,口腔粘膜褶数量和形态不同;食道分段数量不同(史氏鲟分3段、中华鲟分... 详细信息

通过对史氏鲟的活体解剖和对消化系统各组织的观察,结果表明史氏鲟具有胃、幽门盲囊和瓣肠,胰脏形成独立的器官,在肝门部有部分弥散于肝脏组织中。与同属的中华鲟比较,口腔粘膜褶数量和形态不同;食道分段数量不同(史氏鲟分3段、中华鲟分2段);史氏鲟瓣肠有7个螺旋瓣,比中华鲟少;中华鲟的幽门盲囊有17个小盲囊,而史氏鲟只有10～12个小盲囊。

关键词：形态学组织学消化系统史氏鲟

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索氏六须鲶精子的超微结构

水产学报 2000年第4期24卷 302-305页

作者：尹洪滨孙中武刘玉堂潘伟志中国水产科学研究院黑龙江水产研究所!黑龙江哈尔滨150070 东北林业大学野生动物资源学院!黑龙江哈尔滨150040

索氏六须鲶精子由头部、中段和尾部组成。头部呈球形或椭圆球形。核前端无顶体 ,后端有植入窝 ,核中染色质致密 ,有核泡。中段包括中心粒和袖套。近端中心粒由两个半圆形中心粒单体相对组成一个空心球体 ,空心处有一核样致密物质 ;远端... 详细信息

索氏六须鲶精子由头部、中段和尾部组成。头部呈球形或椭圆球形。核前端无顶体 ,后端有植入窝 ,核中染色质致密 ,有核泡。中段包括中心粒和袖套。近端中心粒由两个半圆形中心粒单体相对组成一个空心球体 ,空心处有一核样致密物质 ;远端中心粒呈钟罩形 ,顶端与近端中心粒相连 ,底部开口处与精子尾部轴丝端相连。袖套与细胞核后端相连 ,含有丰富的线粒体和囊泡。尾部主要结构是轴丝 ,为典型的“9+ 2”结构。尾部内没有囊泡及其它细胞器 ,尾部外表面光滑 ,没有鳍样结构。

关键词：索氏六须鲶精子超微结构

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半圈养条件下东北虎繁殖期的行为时间分配

生态学杂志 2003年第6期22卷 53-56页

作者：滕丽微刘振生李枫姜广顺刘丹周晓禹郭玉蓉中国科学院动物研究所北京100080 东北林业大学野生动物资源学院哈尔滨150040 黑龙江东北虎林园哈尔滨150027

1998年 10月～ 2 0 0 0年 10月在黑龙江东北虎林园 ,采用随机取样法和瞬时扫描取样法对繁殖期东北虎的行为进行了研究。结果表明 ,在繁殖期的不同阶段东北虎的行为发生较大的变化。在发情交配期雌雄虎的时间分配相似 (P >0 0 5 ) ,均... 详细信息

1998年 10月～ 2 0 0 0年 10月在黑龙江东北虎林园 ,采用随机取样法和瞬时扫描取样法对繁殖期东北虎的行为进行了研究。结果表明 ,在繁殖期的不同阶段东北虎的行为发生较大的变化。在发情交配期雌雄虎的时间分配相似 (P >0 0 5 ) ,均将最多的时间用于运动 ,用于休息的时间较少 ,用于发情交配和其它行为的时间较多。雌虎在妊娠期较多的时间用于休息和运动 ,取食、站立、社会和其它行为的时间均较少 ;产仔期最多的时间用于休息 ,其次是运动 ,用于产仔和其它行为的时间较多 ,用于取食的时间最少 ;哺乳期最多的时间用于休息 ,其次是哺乳和运动 ,用于取食和其它行为的时间较少。活动规律方面 ,各种行为在强度、出现和持续的时间上存在较大差异。单因素方差分析表明 ,在繁殖期的不同阶段雌性东北虎的休息和运动存在极显著差异 (P 0 0 5 )。 10 0日龄内幼虎用于休息的时间最多 ,其次是运动和取食 ,其它行为的时间最少。

关键词：东北虎行为时间分配活动规律黑龙江东北虎林园

Characteristics of echolocating bats’auditory stereocilia length, compared with other mammals

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Science China(Life Sciences) 2007年第4期50卷 492-496页

作者： YAO Qian1,6, ZENG JinYao2, ZHENG YongMei3, Julia LATHAM4, LIANG Bing1, JIANG Lei3 & ZHANG ShuYi51 Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2 college of wildlife resources, northeast forestry university, harbin 150040, China 3 Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China 4 Zoological Society of London, Institute of Zoology, London, NW1 4RY, England 5 School of Life Science, East China Normal university, Shanghai 200062, China 6 Graduate university of Chinese Academy of Sciences, Beijing 100049, China These authors contributed equally to this work Institute of Zoology Chinese Academy of Sciences Beijing 100080 China Graduate university of Chinese Academy of Sciences Beijing 100049 China college of wildlife resources Northeast Forestry University Harbin 150040 China Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Zoological Society of London Institute of Zoology London NW1 4RY England Institute of Zoology Chinese Academy of Sciences Beijing 100080 China School of Life Science East China Normal University Shanghai 200062 China

The stereocilia of the Organ of Corti in 4 different echolocating bats, Myotis adversus, Murina leuco-gaster, Nyctalus plancyi (Nyctalus velutinus), and Rhinolophus ferrumequinum were observed by using scanning electron microscopy (SEM). Stereocilia lengths were estimated for comparison with those of non-echolocating mammals. The specialized lengths of outer hair cells (OHC) stereocilia in echolocating bats were shorter than those of non-echolocating mammals. The specialized lengths of inner hair cells (IHC) stereocilia were longer than those of outer hair cells stereocilia in the Organ of Corti of echolocating bats. These characteristics of the auditory stereocilia length of echolocating bats represent the fine architecture of the electromotility process, helping to adapt to high frequency sound and echolocation.

关键词： echolocating bats cochlea stereocilia scanning electron microscopy (SEM)

Method of transforming resistance gene to carbendazim into Trichoderma sp.

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Chinese Science Bulletin 1999年第1期44卷 65-68页

作者： YANG Qian ZHAO Xiaoyancollege of forestry, northeast forestry university, harbin 150040, China harbin Veterinary Institute, harbin 150040, China college of forestry Northeast Forestry University Harbin China harbin Veterinary Institute Harbin China

The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed into Trichoderma harzianum, a strain of T. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.

关键词： gene of resistance to carbendazim gene transformation Trichoderma sp.

Pictet-Spengler condensation reactions catalyzed by a recyclable H^+-montmorillonite as a heterogeneous BrΦnsted acid

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Science China Chemistry 2010年第3期53卷 561-567页

作者： WANG YuFeng,SONG ZhanBo,CHEN ChunXia & PENG JinSong Department of Chemistry and Chemical Engineering,college of Science,northeast forestry university,harbin 150040,China Department of Chemistry and Chemical Engineering College of Science Northeast Forestry University Harbin China

Catalytic performance of different cation-exchanged montmorillonite clays has been investigated in the Pictet-Spengler C-C bond forming reaction.H+-Montmorillonite was found to be a very efficient and reusable catalyst for the endo cyclization of β-phenylethylamine derivatives with aldehydes under solvent-free *** addition,an aqueous condensation version for the synthesis of tetrahydro-β-carbolines using the H+-montmorillonite catalyst has been *** these environmentally friendly processes,the use of organic solvents was avoided and the catalyst was recycled with maintenance of high catalytic activity.

关键词： heterogeneous catalysis catalyst recycling mesoporous materials green synthesis