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Deguelin regulates nuclear pore complex proteins Nup98 and Nup88 in U937 cells in vitro1

Acta Pharmacologica Sinica 2005年第10期26卷 1265-1273页

作者： Hong-li LIU~2, Yan CHEN~(2,3), Guo-hui CUI~2, Qiu-ling WU~2, Jing HE~2, Wei-hua CHEN~2, Jian-feng ZHOU~4 ~2 Department of Hematology, Union Hospital, Tongji Medical college, Huazhong university of science and technology, Wuhan 430022 ~4 Department of Hematology, Tongji Hospital, Tongji Medical college, Huazhong university of science and technology, Wuhan 430030, china Department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim: To investigate the anticancer effects and the molecular mechanisms of deguelin on human U937 leukemia cells, and to explore the underlying mechanism regulating nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro. Methods: The effects of deguelin on the growth of U937 cells were studied by MTT assay. The effect of deguelin on the cell cycle of U937 cells was studied by using a propidium iodide method. The localization of the nuclear pore complex proteins Nup98 and Nup88 was investigated by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 and Nup88 in U937 cells was investigated by using flow cytometry and Western blot. Results: The prolif eration of U937 cells was inhibited in the deguelin-treated group, with a 24-h IC50value of 21.61 nmol/L and a 36-h IC50value of 17.07 nmol/L. U937 cells treated with deguelin had reduced percentages of cells in the G0/G1phase, whereas cells accumulated in the S and G2/M phases. Nup88 and Nup98 were found on both the nuclar and cytoplasmic sides of the U937 cells by using immunofluorescence and immunoelectron microscopy. The expression of Nup98 was upregulated and that of the Nup88 protein was downregulated in U937 cells treated with deguelin. Conclusion: Deguelin is able to inhibit the proliferation of U937 cells by regulating the cell cycle such that cells are arrested at the S and G2/M phases, so that the proportion of cells in the G0/G1phase decreases. The antitumor effects of deguelin are related to upregulating the expression of Nup98 and downregulating the expression of Nup88 protein in U937 cells.

关键词： deguelin U937 cells nucleoporin Nup98 Nup88

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Inhibition of ethanol-induced toxicity by tanshinone ⅡA in PC12 cells

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Acta Pharmacologica Sinica 2006年第6期27卷 659-664页

作者： Xian-fang MENG~2 Xiao-jing ZOU~3 Bin PENG~2 Jing SHI~2 Xin-min GUAN~2 Chun ZHANG~(4,5)2 Department of Neurobiology,Tongji Medical college,Huazhong university of science and technology,Wuhan 430030,china 3 Department of Anesthesiology,4 Department of Nephrology,Union Hospital,Tongji Medical college,Huazhong university of science and technology,Wuhan 430022.china Department of Neurobiology Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Nephrology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To observe the effects of tanshinone IIA(Tan IIA)on the neurotoxicityinduced by ethanol in PC 12 cells and to explore its protective ***:PC 12cell survival was measured by MTT *** formation of reactive oxygenspecies(ROS)and lactate dehydrogenase(LDH)release were detected by 2′,7′-dichlorofluorescin(DCF)fluorescence and calorimetric method,*** of cell apoptosis was monitored by flow *** expression ofp53 was detected by immuno-fluorescence and flow ***:Ethanolsignificantly impaired the survival of PC12 cells as demonstrated by MTT *** also induced significant ROS formation and increased LDH ***-incubation with Tan IIA in the culture medium significantly reversed these *** caused cell apoptosis and the upregulation of p53 *** anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by thedownregulation of pro-apoptotic p53 protein ***:Tan IIAcan protect neurons from apoptosis and might serve as a potential therapeuticdrug for neurological disorders induced by ethanol.

关键词： apoptosis ethanol PC12 cells p53 tanshinone ⅡA

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Complex variable method for plane elasticity of icosahedral quasicrystals and elliptic notch problem

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science china(Physics,Mechanics & Astronomy) 2008年第7期51卷 773-780页

作者： LI LianHe1,2 & FAN TianYou1 1 Department of Physics, Beijing Institute of technology, Beijing 100081, china 2 college of Mathematics science, Inner Mongolia Normal university, Hohhot 010022, china Department of Physics Beijing Institute of Technology Beijing China college of Mathematics science Inner Mongolia Normal University Hohhot China

The complex variable method for the plane elasticity theory of icosahedral quasicrystals is developed. Based on the general solution obtained previously, complex representations of stress and displacement components of phonon and phason fields in the quasicrystals are given. With the help of conformal transformation, an analytic solution for the elliptic notch problem of the material is presented. The solution of the Griffith crack problem can be observed as a special case of the results. The stress intensity factor and energy release rate of the crack are also obtained.

关键词： icosahedral quasicrystals plane elasticity elliptic notch complex variable method

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Analysis on the positioning precision of CAPS

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science china(Physics,Mechanics & Astronomy) 2009年第3期52卷 328-332页

作者： JI YuanFa1,2 & SUN XiYan1,2 1 National Astronomical Observatories, Chinese Academy of sciences, Beijing 100012, china 2 Applied science and technology college, Guilin university of Electronic technology, Guilin 541004, china National Astronomical Observatories Chinese Academy of Sciences Beijing China Applied science and technology college Guilin University of Electronic Technology Guilin China

As a newly developed satellite positioning system, the Chinese Area Positioning System (CAPS) is a typical direct sequence spread spectrum ranging system like GPS. The positioning precision of such navigation signals depends on many factors, including the pseudo-code rate, the signal to noise ratio, the processing methods for tracking loops and so on. This paper describes the CAPS link budget, the solution approach for CAPS positioning, focusing on the autocorrelation function feature of C/A code signals. The CAPS signal measurement precision is studied by the software approach together with theoretical analysis of the range resolution. Because the conventional Delay Lock Loop (DLL) is vul- nerable to the impact of noise, a narrow correlator and multiple correlators as well as the corresponding discrimination methods of phases are proposed, which improves the robustness of DLL and the code-phase resolution of the measurement. The results show that the improvement of the DLL structure and the discrimination method are the most important way to improve the ranging resolution. Theoretical analysis and experimental results show that a CAPS receiver could reach a 20-m positioning precision by using three satellites with a supported height from an altimeter.

关键词： satellite navigation receiver narrow correlation tracking loop

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A NOVEL INTERPRETATION OF CONCENTRATION DEPENDENCE OF VISCOSITY OF DILUTE POLYMER SOLUTION

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Chinese Journal of Polymer science 2000年第1期18卷 57-67页

作者： Yan Pan Rong-shi Cheng college of Material science and Engineering, South china university of technology, Guangzhou 510641, china college of Chemistry and Chemical Engineering, Nanjing university, Nanjing 210093, china 华南理工大学广东广州

The concentration dependence of the reduced viscosity of dilute polymer solution is interpreted in the light of a new concept of the self-association of polymer chains in dilute solution. The apparent self-association constant is defined as the molar association constant divided by the molar mass of individual polymer chain and is numerically interconvertible with the Huggins coefficient. The molar association constant is directly proportional to the effective hydrodynamic volume of the polymer chain in solution and is irrespective of the chain architecture. The effective hydrodynamic volume accounts for the non-spherical conformation of a short polymer chain in solution and is a product of a shape factor and hydrodynamic volume. The observed enhancement of Huggins coefficient for short chain and branched polymer is satisfactorily interpreted by the concept of self-association. The concept of self-association allows us to predict the existence of a boundary concentration C-s(dynamic contact concentration) which divides the dilute polymer solution into two regions.

关键词： self-association polymer solution viscosity concentration dependence

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Comprehensive benefit of flood resources utilization through dynamic successive fuzzy evaluation model: A case study

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中国科学：技术科学英文版 2010年第2期53卷 529-538页

作者： FENG Feng1,2, XU ShiGuo1, LIU JianWei1, LIU Dan1,3 & WU Bo4 1 Institute of Water Environment, School of Civil and Hydraulic Engineering, dalian university of technology, dalian 116024, china 2 Yellow River Conservancy Technical Institute, Kaifeng 475003, china 3 college of Water Resources, Shenyang Agricultural university, Shenyang 110161, china 4 Office of Flood Control and Draught Relief in Jilin Province, Changchun 130033, china Institute of Water Environment School of Civil and Hydraulic Engineering Dalian University of Technology Dalian China Yellow River Conservancy Technical Institute Kaifeng China college of Water Resources Shenyang Agricultural University Shenyang China Office of Flood Control and Draught Relief in Jilin Province Changchun China

Taking the flood resources utilization in Baicheng, Jilin during 2002–2007 as the research background, and based on the entropy weight and multi-level & multi-objective fuzzy optimization theory, this research established a multi-level & semi-constructive index system and dynamic successive evaluation model for comprehensive benefit evaluation of regional flood resources utilization. With the year 2002 as the base year, the analyzing results showed that there existed a close positive correlation between flood utilization volume and its benefits, comprehensive evaluation value and its comparison increment. Within the six successive evaluation years, the comprehensive benefit of 2003 was the best, in which the benefit evaluation increment reached 82.8% whereas the year of 2004 was the worst, in which the increment was only 18.2%. Thus the sustainability and correctness of the evaluation were verified by six years successive evaluation and increment comparison. The analyzing results showed that the economic benefits, ecological benefits and social benefits of flood utilization were remarkable, and that the comprehensive benefit could be improved by increasing flood utilization capacity, which would promote the regional sustainable development as well. The established dynamic successive evaluation provides a stable theoretical basis and technical support for further flood utilization.

关键词： flood resources utilization comprehensive benefit dynamic successive fuzzy evaluation Baicheng City

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Effect of D-allethrin on proteinase activity in the midgut of the spider Pardosa pseudoannulata detected by bulk acoustic wave impedance technique

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Progress in Natural science:Materials International 2007年第12期17卷 1521-1526页

作者： Wang Zhi1, Fu Xiuqin2, Rao Liqun1 and Lu Xiangyang1(1. college of Bioscience & Biotechnology, Hunan Agriculture university, Changsha 410128, china 2. college of life science, Hunan Normal university, Changsha 410081, china) college of Bioscience and Biotechnology Hunan Agriculture University Changsha 410128 China college of life science Hunan Normal University Changsha 410081 China

The piezoelectric bulk acoustic wave (BAW) impedance analysis method was employed to monitor in situ the proteinase-catalyzed hydrolyzation of casein and the effect of pesticide D-allethrin on the proteinase activity in the midgut of the spider Pardosa pseudoannulata, and the results obtained were verified by ultraviolet spectroscopy. The hydrolyzation was significantly increased in the presence of low-dose D-allethrin, whereas significantly inhibited in the presence of high-dose D-allethrin, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of D-allethrin, with a significant frequency response observed even at a 1.5×10-5 volume ratio of D-allethrin to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose D-allethrin pesticide and quick detection of proteinase activity.

关键词： Pardosa pseudoannulata D-allethrin proteinase activity ultraviolet spectroscopy piezoelectric bulk acoustic wave impedance method.

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Noether-Form Invariance of Nonholonomic Controllable Mechanical Systems in Phase Space

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Communications in Theoretical Physics 2007年第7期48卷 23-26页

作者： XIA Li-Li LI Yuan-Cheng college of Physics science and technology,china university of Petroleum,Dongying 257061,china college of Physics science and technology China University of Petroleum Dongying 257061 China

In this paper,we study the Noether-form invariance of nonholonomic mechanical controllable systems inphase *** of motion of the controllable mechanical systems in phase space are *** definitionand the criterion for this system are presented.A new conserved quantity and the Noether conserved quantity deducedfrom the Noether-form invariance are *** example is given to illustrate the application of the results.

关键词： nonholonomic controllable mechanical system phase space Noether-form invariance conserved quantity

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ON THE LEAST SQUARES PROBLEM OF A MATRIXEQUATION

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Journal of Computational Mathematics 1999年第6期17卷 589-594页

作者： An-ping Liao(college of science, Hunan Normal university, Changsha 410081, china) 湖南师范大学理学院湖南长沙 410081

Least squares solution of F=PG with respect to positive semidefinite symmetric P is considered,a new necessary and sufficient condition for solvablity is given,and the expression of solution is derived in the some special cases. Based on the expression, the least spuares solution of an inverse eigenvalue problem for positive semidefinite symmetric matrices is also given.

关键词： least squares solution matrix equation inverse eigenvalue problem positive semidefinite symmetric matrix

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Dynamic Mechanical Behaviour of Ultra-high Performance Fiber Reinforced Concretes

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Journal of Wuhan university of technology(Materials science) 2008年第6期23卷 938-945页

作者：赖建中 Department of Materials science & Engineering Nanjing University of Science & Technology

Ultra-high performance fiber reinforced concretes （UHPFRC） were prepared by replacing 60% of cement with ultra-fine industrial waste powder. The dynamic mechanical behaviour of UHPFRC with different fiber volume fraction was researched on repeated compressive impact in four kinds of impact modes through split Hopkinson pressure bar （SHPB）. The experimental results show that the peak stress and elastic modulus decrease and the strain rate and peak strain increase gradually with the increasing of impact times. The initial material damage increases and the peak stress of the specimen decreases from the second impact with the increasing of the initial incident wave. Standard strength on repeated impact is defined to compare the ability of resistance against repeated impact among different materials. The rate of reduction of standard strength is decreased by fiber reinforcement under repeated impact. The material damage is reduced and the ability of repeated impact resistance of UHPFRC is improved with the increasing of fiber volume fraction.

关键词： ultra-high performance fiber reinforced concretes split Hopkinson pressurebar dynamic repeated impact

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