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Use of the modified viral satellite DNA vector to silence mineral nutrition-related genes in plants: silencing of the tomato ferric chelate reductase gene, FRO1, as an example

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science china(life sciences) 2008年第5期51卷 402-409页

作者： HE XiuXia1,2,JIN ChongWei1, LI GuiXin3, YOU GuangYi4, ZHOU XuePing3 & ZHENG ShaoJian4 1 Key Laboratory for Environmental and Ecosystem Health of the Ministry of Education, college of Environmental and Resource science, Zhejiang university, Hangzhou 310029, china 2 college of life science, changchun university of science and technology, changchun 130022, china 3 Institute of Biotechnology, Zhejiang university, Hangzhou 310029, china 4 State Key Laboratory of Plant Physiology and Biochemistry, college of life science, Zhejiang university, Hangzhou 310058, china 1. Key Laboratory for Environmental and Ecosystem Health of the Ministry of Education College of Environmental and Resource Science Zhejiang University Hangzhou 310029 China 2. college of life science Changchun University of Science and Technology Changchun 130022 China 3. Institute of Biotechnology Zhejiang University Hangzhou 310029 China 4. State Key Laboratory of Plant Physiology and Biochemistry College of Life Science Zhejiang University Hangzhou 310058 China

Virus-induced gene silencing (VIGS) is potentially an attractive reverse-genetics tool for studies of plant gene function, but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. Here we report on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmβ) associated with Tomato yellow leaf curl china virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FRO1) from tomato was inserted into the DNAmβ vector. Tomato roots agro-inoculated with DNAmβ carrying both a fragment of FRO1 and TYLCCNV used as a helper virus exhibited a significant reduction at the FRO1 mRNA level. As a consequence, ferric chelate reductase activity, as determined by visualization of the pink FeBPDS3 complex was significantly decreased. Our results clearly demonstrated that VIGS system can be employed to investigate gene function associated with plant nutrient uptake in roots.

关键词： virus-induced gene silencing roots ferric chelate reductase

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Towards advanced zinc anodes by interfacial modification strategies for efficient aqueous zinc metal batteries

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Journal of Energy Chemistry 2024年第6期93卷 79-110,I0003页

作者： changchun Fan Weijia Meng Jiaye Ye Key Laboratory of Bio-Inspired Smart Interfacial science and technology Ministry of EducationSchool of ChemistryBeihang UniversityBeijing 100191China Shaanxi Key Laboratory of New Transportation Energy and Automotive Energy Saving School of Energy and Electrical EngineeringChang’an UniversityXi’an 710061ShaanxiChina School of Chemistry and Physics Faculty of ScienceQueensland University of TechnologyBrisbaneQLD 4001Australia Centre for Materials science Queensland University of TechnologyBrisbaneQLD 4001Australia

Developing sustainable and clean energy sources(e.g.,solar,wind,and tide energy)is essential to achieve the goal of carbon *** to the discontinuous and inco nsistent nature of common clean energy sources,high-performance energy storage technologies are a critical part of achieving this *** zinc metal batteries(AZMBs)with inherent safety,low cost,and competitive performance are regarded as one of the promising candidates for grid-scale energy ***,zinc metal anodes(ZMAs)with irreversible problems of dendrite growth,hydrogen evolution reaction,self-corrosio n,and other side reactions have seriously hindered the development and commercialization of *** increasing number of researchers are focusing on the stability of ZMAs,so assessing the effectiveness of existing research strategies is critical to the development of *** review aims to provide a comprehensive overview of the fundamentals and challenges of *** rch strategies for interfacial modification of ZMAs are systematically *** features of artificial interfacial coating and in-situ interfacial coating of ZMAs are compared and discussed in detail,as well as the effect of modified interfacial ZMA on the full-battery ***,perspectives are provided on the problems and challenges of *** review is expected to offer a constructive reference for the further development and commercialization of AZMBs.

关键词： Aqueous zinc metal batteries Zinc metal anode Interfacial modification Artificial interfacial coating In-situ interfacial coating

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Quantifying quantum entanglement via machine learning models

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Communications in Theoretical Physics 2024年第7期76卷 52-56页

作者： changchun Feng Lin Chen LMIB(Beihang university) Ministry of EducationBeijing 100191China School of Mathematical sciences Beihang UniversityBeijing 100191China International Research Institute for Multidisciplinary science Beihang UniversityBeijing 00191China

Quantifying entanglement measures for quantum states with unknown density matrices is a challenging *** learning offers a new perspective to address this *** training machine learning models using experimentally measurable data,we can predict the target entanglement *** this study,we compare various machine learning models and find that the linear regression and stack models perform better than *** investigate the model's impact on quantum states across different dimensions and find that higher-dimensional quantum states yield better ***,we investigate which measurable data has better predictive power for target entanglement *** correlation analysis and principal component analysis,we demonstrate that quantum moments exhibit a stronger correlation with coherent information among these data features.

关键词： quantum information entanglement predict machine learning coherence

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Finite-time composite learning control for nonlinear teleoperation systems under networked time-varying delays

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science china(Information sciences) 2024年第6期67卷 279-296页

作者： Yana YANG Huixin JIANG changchun HUA Junpeng LI Institute of Electrical Engineering Yanshan University

The robust finite-time synchronization control problem is investigated for master-slave networked nonlinear telerobotics systems(NNTSs) in this article. Although there have been some research achievements on finite-time control for the NNTSs, these studies are based on the strong assumptions of communication time delays or can only achieve finite-time bounded convergence even when the external forces are *** and in view of the importance of these issues, a novel robust composite learning adaptive control scheme rendering the finite-time master-slave synchronization is proposed in this paper. In particular, the influence of time delays on finite-time convergence of the system is analyzed by employing the multi-dimension finite-time small-gain framework. Meanwhile, in order to achieve accurate and fast estimation of uncertain parameters of the system, both the online historical and the instantaneous data of the estimation data are explored to derive the new parameter adaptive law under a more realizable interval-excitation(IE) ***, the convergence of the position/force synchronization errors and the adaptive parameter estimation errors is obtained in finite time, and enhanced robustness of the closed-loop system will also be ***, the superior performance of the proposed control algorithms is validated by numerical simulations and hardware experiments.

关键词： networked teleoperation systems finite-time control exact parameter estimation small-gain theorem unknown communication delays

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Characterizing structural variants based on graph-genotyping provides insights into pig domestication and local adaption

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Journal of Genetics and Genomics 2024年第4期51卷 394-406页

作者： Xin Li Quan Liu Chong Fu Mengxun Li changchun Li Xinyun Li Shuhong Zhao Zhuqing Zheng Key Lab of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of AgricultureCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China Institute of Agricultural Biotechnology Jingchu University of TechnologyJingmenHubei 448000China The Cooperative Innovation Center for Sustainable Pig Production WuhanHubei 430070China Hubei Hongshan Laboratory WuhanHubei 430070China

Structural variants(SVs),such as deletions(DELs)and insertions(INSs),contribute substantially to pig genetic diversity and phenotypic *** a library of SVs discovered from long-read primary assemblies and short-read sequenced genomes,we map pig genomic SVs with a graph-based method for re-genotyping SVs in 402 *** results demonstrate that those SVs harboring specific trait-associated genes may greatly shape pig domestication and local *** characterization of SVs reveals that some population-stratified SVs may alter the transcription of genes by affecting regulatory *** identify that the genotypes of two DELs(296-bp DEL,chr7:52,172,101e52,172,397;278-bp DEL,chr18:23,840,143 e23,840,421)located in muscle-specific enhancers are associated with the expression of target genes related to meat quality(FSD2)and muscle fiber hypertrophy(LMOD2 and WASL)in *** results highlight the role of SVs in domestic porcine evolution,and the identified candidate functional genes and SVs are valuable resources for future genomic research and breeding programs in pigs.

关键词： Pigs Structural variation Selection signatures Regulatory elements

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Portable fluorescence instrument for detecting membrane integrity in membrane bioreactor (MBR)

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Frontiers of Environmental science & Engineering 2024年第2期18卷 121-131页

作者： Yang Yu changchun Xin Yuxiang Liu Fei Gao Lei Zhang Hui Jia Jie Wang State Key Laboratory of Separation Membranes and Membrane Processes Tiangong UniversityTianjin 300387China School of Environmental science and Engineering Tiangong UniversityTianjin 300387China School of Environmental science and Engineering Tianjin UniversityTianjin 300072China Shenyang Academy of Environmental sciences Shenyang 110167China Cangzhou Institute of Tiangong university Tiangong UniversityCangzhou 061000China

This study proposed the design, fabrication, and assembly of membrane integrity detection instruments in membrane bioreactors (MBR) based on fluorescence spectroscopy. Based on the PARAFAC model, we found that the peak at 280/335 nm strengthened after membrane breakage. The peak at 340/430 nm reflected the sludge concentration in the MBR and reduced the influence of internal filtration effects on detection. Therefore, we determined that the dual-LED light source excitation detection system can detect tryptophan-like substances at 280 nm (T-peak) and humic acid at 340 nm (C-peak). T-peak was identified as the core index indicating membrane integrity. Moreover, the C-peak is the reference indicator factor for a sensitive response to changes in the sludge concentration. The portable fluorescence instrument exhibited high sensitivity and good feedback accuracy compared to particle counting and turbidity detection, where the log reduction value was greater than 3.5. This overcomes the disadvantage of false alarms in particle counters and is not affected by the position of the pump system. This portable instrument provides a flexible and highly sensitive method for the assessment of industrial membrane integrity.

关键词： Membrane integrity Fluorescence spectrum Portable instrument Membrane bioreactor

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Kinetics analysis of volume phase transition of intelligent neutral thermo-sensitive hydrogels

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science china(Physics,Mechanics & Astronomy) 2008年第5期51卷 532-540页

作者： WANG XiaoGui1 & LI YiQuan2 1 college of Mechanical Engineering,Zhejiang university of technology,Hangzhou 310032,china 2 School of Mechanical & Electronic Engineering,changchun university of science and technology,changchun 130022,china 1. college of Mechanical Engineering Zhejiang University of Technology Hangzhou 310032 China 2. School of Mechanical & Electronic Engineering Changchun University of Science and Technology Changchun 130022 China

In this work,utilizing the first law of thermodynamics and the Flory mean-field theory,the kinetic deformation studies concerning the volume phase transition of the neutral thermo-sensitive hydrogels were performed *** hydrogel was assumed as a biphasic mixture medium in the framework of the continuum mixture *** the energy conservation of the thermodynamics system of the hydrogel,the governing equations for the kinetics of the nonlinear large deforma-tion were *** explicit analytical expressions of the effective stress tensor and the chemical potential of the fluid of the thermo-sensitive hydrogel PNIPA were also obtained from the Helmholtz free energy,which can model the steady-static volume phase transition quantitatively.

关键词： thermo-sensitive hydrogel volume phase transition effective stress energy conservation

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The degenerating diffraction far-field propagation properties of the conical double half-Gaussian hollow beams

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science china(Physics,Mechanics & Astronomy) 2010年第3期53卷 486-490页

作者： DONG Yuan, ZHANG XiHe, JIN GuangYong, LING Ming & NING GuoBin School of science, changchun university of science and technology, changchun 130022, china 1. School of science Changchun University of Science and Technology Changchun 130022 China

The far-field propagation properties of conical double half-Gaussian hollow beams in the condition of Collins formula are studied. Because of the cone angle of this kind of hollow beams, the diffraction is compensated and the inner diameter is turning bigger by the rule of geometric optics as the propagation distance is increasing, whereas the degenerating diffraction phenomenon is turned out. The far-field intensity distribution of the conical double half-Gaussian hollow beams in the condition of in-Collins formula is researched, and the results show that the far-field propagation properties can be depicted by this model. In the experiment, this kind of hollow beams are obtained by means of the dual-reflecting splitting optical system, and the inner diameter of the hollow beams is tested. The results show good agreement with the propagation theory in the condition of in-Collins formula.

关键词： conical double half-Gaussian hollow beams propagation properties the condition of in-Collins formula degenerating diffraction

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Study of Laser Lap Welding of SUS301L Austenitic Stainless Steel Using Optimal Design Method

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稀有金属材料与工程 2011年第S4期40卷 63-67页

作者： Liu Jia,Shi Yan,Zhang Hong School of Electromechanical Engineering,changchun university of science and technology,changchun 130022,china changchun Univ Sci & Technol Sch Electromech Engn Changchun 130022 Peoples R China

This paper presents an experimental design approach to process parameter optimization for the laser lap welding of SUS301L austenitic stainless steels using Nd:YAG laser in order to reduce the welding deformations of the back of the welding seams while ensuring mechanical properties of welding *** determine the optimal laser-welding parameters,a set of mathematical models were developed relating welding parameters to each of the weld *** were validated both statistically and *** order to reduce experimental error and the number of specimens,the part of orthogonal experiments were used in this *** quality criteria set for the weld to determine optimal parameters were the maximization of weld width and the minimization of weld *** power,welding speed,the laser irradiation angle,the focused distance and shield gas of 3.5kW,7.0m/min,70°,0mm and N2 with the flow of 30L/min,respectively,with a fiber diameter of 600μm were identified as the optimal set of process parameters.

关键词： laser lap welding sus301l austenitic stainless steel welding deformation orthogonal experiments

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pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： Wen-Ying LI2, Ji WANG1, Yan-Hong LI1, Ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, the Chinese Academy of sciences,Graduate School of the Chinese Academy of sciences, Shanghai 200031, china 2college of life science, Jilin university, changchun 130023, china college of life science, Zhejiang university of science and technology, Hangzhou 310018, china Key Laboratory ofProteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

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