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Nitric Oxide Inducing Function and Intracellular Movement of Chicken Interleukin-18 in Cultured Cells

Acta Biochimica et Biophysica Sinica 2005年第10期37卷 688-693页

作者： Jian XU~(1,3) Tong-Le DENG~2 Long LI~1 Zhen-Qiang YOU~1 Wang-Jun WAN~2 Lian YU~(1*) 1 zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine.Institute of Preventive Veterinary Medicine,zhejiang university,hangzhou 310029.china 2 college of biomedical engineering and instrument science,zhejiang university,hangzhou 310029,china 3 Medical college of Shihezi university,Xinjiang 832002,china zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China Medical college of Shihezi universityXinjiang 832002 China college of biomedical engineering and instrument science Zhejiang UniversityHangzhou 310029 China

To evaluate the characteristics of chicken interleukin-18 (ChIL-18) in different forms in vitro,the ChIL-18 full-length gene (ChIL-18-F) and the ChIL-18 presumed mature protein gene (ChIL-18-M) werecloned and inserted into the eukaryotic expression vector pCI,to construct recombinant pCI-ChIL-18-Fand *** recombinant plasmids were then transferred into chicken splenic lymphocytes(CSLs).Western blot showed that ChIL-18-F,with a molecular weight of 23.0 kDa,was produced in CSLstransfected by pCI-ChlL-18-F;ChIL-18-M,with a molecular weight of 19.5 kDa,was produced in CSLstransfected by *** nitric oxide (NO) level in the transfected CSLs and the culture mediumat different time points was further examined under confocal microscopy using *** results showed that both pCI-ChIL-18-F and pCI-ChIL-18-M groups showed significantincrease in intracellular and extracellular NO production compared with pCI transfected control *** suggest that both ChIL-18-F and ChIL-18-M could stimulate NO secretion in *** characterizethe intracellular distribution of ChIL-18,ChIL-18-F and ChIL-18-M were each fused to the enhanced greenfluorescent protein gene,and expressed in Vero *** results showed that the ChIL-18-F tended to themembranous region in Vero cells,while ChIL-18-M did *** indicates that the N-terminal 27 amino acidpeptide helped ChIL-18 target to Veto cell membranes.

关键词： chicken interleukin-18 N-terminal peptide nitric oxide splenic lymphocyte intracellular movement

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Positive Effect of Noises on Sensory Systems

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Journal of Bionic engineering 2004年第1期1卷 61-65页

作者： Jun Liu, Guang Li Institute of biomedical engineering and instrument science, zhejiang university, hangzhou 310027,P.R.china Institute of biomedical engineering and instrument science Zhejiang University Hangzhou 310027 P. R. China

Stochastic resonance phenomenon in the biological sensory systems has been studied through the signal detection theories and the psychophysical experiments. In this paper, sensory systems are considered as a threshold detector including the receiver part and the classifier part. Compared with conventional models regarding the receiver part of sensory system as a linear or single non-linear system, a summing network was constructed by MacCulloch-Pitts neurons to simulate the receiver part. The simulation results show that the relevant index of the detectability of signal exhibit the stochastic resonance behaviours. The psychophysical experiments were carried out through the 2IFC (two interval two alternative forced choice) method. The experimental results qualitatively verify the conclusion in accordance with the theoretical model. These works give a proof that stochastic resonance is not only epiphenonmenon in sensory systems.

关键词： stochastic resonance signal detection theory psychophysical experiment MacCulloch-Pitts neuron

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State space approach for joint estimation of activity and attenuation map from PET emission sinograms

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Progress in Natural science:Materials International 2007年第5期17卷 614-617页

作者： Liu Huafeng , You Hongshun and Shi Pengcheng (State Key Laboratory of Modern Optical instrumentation, zhejiang university, hangzhou 310027, china college of biomedical engineering, Southern Medical university, Guangzhou 510015, china) State Key Laboratory of Modern Optical instrumentation Zhejiang University Hangzhou 310027 China college of biomedical engineering Southern Medical University Guangzhou 510015 China

Quantitative estimation of radioactivity map has important clinical implications for better diagnosis and understanding of cancers. Although attenuation map and activity map are usually treated sequentially, they can obviously benefit a great deal when the transmission data is missing. In this paper, we propose a novel scheme of simultaneously solving for attenuation map and activity distribution from emission sinograms. Our strategy combines the measurement model of PET, and the attenuation parameters are treated as random variables with known prior statistics. After the conversion to state space representation, the extended Kalman filtering procedures are adopted to linearize the equations and to provide the joint estimates in an approximate optimal sense. Experiments have been performed on both synthetic data to illustrate its abilities and benefits.

关键词： positron emission tomography attenuation correction state space methods.

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Research on Suprathreshold Stochastic Resonance of FitzHugh-Nagumo Neuron Model

Research on Suprathreshold Stochastic Resonance of FitzHugh-...

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2008 IEEE International Symposium on IT in Medicine and Education(ITME 2008)

作者： XUE Lingyun 1 2 LI Meng 2 FAN Yingle 21.Key Laboratory of biomedical engineering of Ministry of Education college of biomedical engineering and instrument science zhejiang university china 2.Institute of biomedical engineering & instrument hangzhou Dianzi university china

Nowadays,noise was considered to play a key role in the neural system,which improved the weak signal detection and enhanced the information transmission,as deeply research on stochastic resonance （SR）.Traditionally,stochastic resonance was thought to be the result of the interaction between subthreshold signals with noise in nonlinear ***,the existence of noise was thought to be harmful to the response of system with suprathreshold signal input *** the response of FitzHugh-Nagumo （FHN） neuron model to the suprathreshold input,it was improved gradually as the amplitude of input rising from subthreshold to ***-to-noise ratio and cross correlation coefficient were defined as performance evaluation functions separately,then the response of FHN neuron model to periodic and aperiodic suprathreshold signal input was *** the same time,the threshold characteristic was discussed,and some basic parameters for practical application were *** simulation result illustrates the information transmission can be enhanced by the noise even if input signal is suprathreshold in FHN neuron model.

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DNA computing model based on lab-on-a-chip and its application on solving the timetabling problem

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Progress in Natural science:Materials International 2008年第5期18卷 633-637页

作者： Fengyue Zhang a,1, Bo Liu b,1, Wenbin Liu c, Qiang Zhang d a Department of biomedical engineering, School of Life science and Technology, Beijing Institute of Technology, Beijing 100081, china b Chinese Academy of Inspection and Quarantine, Beijing 100029, china c college of Computer science and engineering, Wenzhou university, Wenzhou, zhejiang 325027, china d Liaoning Key Laboratory of Intelligent Information Processing, Dalian university, Dalian, Liangning 116622, china Department of biomedical engineering School of Life Science and Technology Beijing Institute of Technology Beijing 100081 China Chinese Academy of Inspection and Quarantine Beijing 100029 China college of Computer science and engineering Wenzhou University Wenzhou Zhejiang 325027 China Liaoning Key Laboratory of Intelligent Information Processing Dalian University Dalian Liangning 116622 China

The essential characteristic of DNA computation is its massive parallelism in obtaining and managing information. With the develop- ment of molecular biology technique, the eld of DNA computation has made a great progress. By using an advanced biochip technique, laboratory-on-a-chip, a new DNA computing model is presented in the paper to solve a simple timetabling problem, which is a special ver- sion of the optimization problems. It also plays an important role in education and other industries. With a simulated biological experiment, the result suggested that DNA computation with lab-on-a-chip has the potential to solve a real complex timetabling problem.

关键词： DNA computing Timetabling problem Lab-on-a-chip

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大鼠脑皮层血管栓塞和再生过程的实时动态记录方法

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生理学报 2019年第4期71卷 581-587页

作者：丁惠洁刘珂舟杨珊珊李鹏严明杭州电子科技大学生命信息与仪器工程学院杭州310018 浙江大学光学科学与工程学院杭州310027 浙江大学生物医学工程与仪器科学学院杭州310027

本研究旨在建立观察脑皮层血管堵塞、脑缺血损伤区血管再生、重构的动态变化过程的方法。创建能够在体连续观测大鼠皮层血管缺血后变化的动物光窗模型,并用光化学栓塞法建立大鼠皮层局部脑缺血模型,通过光相干层析成像术(optical cohere... 详细信息

本研究旨在建立观察脑皮层血管堵塞、脑缺血损伤区血管再生、重构的动态变化过程的方法。创建能够在体连续观测大鼠皮层血管缺血后变化的动物光窗模型,并用光化学栓塞法建立大鼠皮层局部脑缺血模型,通过光相干层析成像术(optical coherence tomography, OCT)实时记录血管堵塞的形成过程以及周围小血管的损伤、再生情况。结果显示,激光照射30 min可以形成完全堵塞大鼠皮层血管的栓塞;缺血后24~48 h内,脑损伤程度最大,损伤区域内血管数量达到最少,随后堵塞的主血管开始疏通,缺血区域内周围小血管开始再生;激光照射后皮层浅层/深层的小血管显著消失,在照射后第10天,皮层浅层出现大量新生血管,而深层血管仍未恢复。以上结果表明,本研究建立的方法可以进行不间断地长期观测,为深入定量研究脑缺血恢复期间栓塞血管及周边毛细血管的动态奠定了基础,为损伤区血管重构网络调控模式研究提供帮助,为脑缺血性疾病的治疗提供理论指导和新研究方法。

关键词：光化学栓塞法局部脑缺血光相干层析成像血管再生

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Minimum-norm Source Estimation of Magnetoencephalography Utilizing Tikhonov Regularization Technique

Minimum-norm Source Estimation of Magnetoencephalography Uti...

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CCAST“北京第二次医学影像物理和工程”国际研讨会

作者： Hu Jie Hu JingFirst Affiliated Hospital zhejiang university college of Medicine hangzhou china 310006 college of Information engineering zhejiang university of Technology hangzhou china

＜正＞ In neuromagnetic source reconstruction, according to the dipole source model and distributed source model, there have two main classes of reconstruction techniques—parametric current dipole localization and nonparametric source reconstruction. MEG inverse problem base on distributed source model becomes an ill-posed undetermined linear problem. In this paper, In order to yield a unique and stable solution, we propose the minimum-norm estimation with Tikhonov regularization technique in order to yield an robust and plausible neural current distribution. We propose a modified regularization method, comparing its performance with the conventional method, computer simulation analysis show how the solution is improved.

关键词： MEG distributed source minimum-norm estimation Tikhonov regularization

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A Novel Method of Signal Enhancement Based on Modified FastICA Algorithm

A Novel Method of Signal Enhancement Based on Modified FastI...

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ICEMI'2005第七届国际电子测量与仪器学术会议

作者： Wu Lili1 Chen Yuquan1 Huang Pinggao2 Mao Yuru3 Zhang Baojun4 (1State Specialized Laboratory of biomedical Sensors college of biomedical engineering and instrument science zhejiang university hangzhou 310027 PR china (2Guilin university of electronic Technology Guilin 541004 china) (3Chinese Research Academy of Environmental sciences Beijing 100012 china) (4college of Computer science zhejiang university hangzhou 310027 china)

Independent component analysis (ICA) has attracted much attention in the field of signal processing. A novel method of speech signal enhancement by using modified FastICA algorithm is presented in thi

关键词： Independent component analysis (ICA) modified FastICA algorithm signal enhancement Newton iteration.

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MEG inversion using spherical head model combined with brain-shaped head model

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Chinese Journal of biomedical engineering(English Edition) 2001年第1期10卷 20-26页

作者： LI Jun State Key Laboratory of Modern Optical instrumentation, Center for Optical and Electromagnetic Research, college of Information science and engineering, zhejiang university, hangzhou 310027, china State Key Laboratory of Modern Optical instrumentation Center for Optical and Electromagnetic Research College of Information Science and EngineeringZhejiang University Hangzhou 310027 China

The spherical head model has been widely used in magnetoencephalography (MEG) as a simple forward model for calculating the external magnetic field producing by neural currents in a human brain. But this model may lead to an inaccurate result, even if the computation speed is fast. For more precise computation, realistic brain-shaped head model is used with the boundary element method (BME), but at greatly increased computational cost. When solving MEG inverse problem by using optimization methods, the forward problem must often be solved for thousands of possible source configurations. So if the brain-shaped head model is used in all iterative steps of optimization, it may be computationally infeasible for practical application. In this paper, we present a method about using compound head model in MEG inverse solution. In this method, first spherical head model is used for a rough estimation, then brain-shaped head model is adopted for more precise solution. Numerical simulation indicates that under the condition of same accuracy, the computation speed for the present method is about three times faster than a method using the brain-shaped head model at all iterations.

关键词： Magnetoencephalography (MEG), Boundary element method, In verse problem, Optimization

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Measurement of the instantaneous oil-consumption in automotive engine

Measurement of the instantaneous oil-consumption in automoti...

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ICEMI'2005第七届国际电子测量与仪器学术会议

作者： Chen Hongyan1 Qiu Zhengjun2 Yang Qihua1 Xu Lijun1 (1:Mechatronics engineering Institute china Jiliang university 310028 hangzhou china 2:college of Bios stems engineering and Food science zhejiang university 310029 china)

A method for the measurement of the oil- consumption ratio of an internal-combustion engine in weight- lost way has been advanced, by which an intelligent measurement instrument with the core of micro

关键词： Measurement oil-consumption engine

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