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IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1

chinese Science Bulletin 2009年第16期54卷 2786-2793页

作者： JIA XiaoLing1,2,3. CHEN Juan1,2 & LONG Mian1,2 1 National Microgravity Laboratory, institute of mechanics, chinese academy of sciences, Beijing 100190, China 2 center of{3. and bioengineering, institute of mechanics, chinese academy of sciences, Beijing 100190, China 3.School of Biological Science and Medical Engineering, Beihang University, Beijing 100083. China National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China center of biomechanics and bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China School of Biological Science and Medical Engineering Beihang University Beijing 100083 China

L-selectin plays a crucial role in inflammation cascade by initiating the tethering and rolling of leukocytes on endothelium wall. While many L-selectin molecules are rapidly shed from the cell surface upon activation, the remaining membrane-anchored L-selectin may still play an important role in regulating leukocyte rolling and adhesion with different binding kinetics. Here we developed an in vitro model to activate Jurkat cells via interlukin-8 (IL-8) and quantified the two-dimensional (2D) binding kinetics, using a micropipette aspiration assay, of membrane-anchored L-selectin to P-selectin glycoprotein ligand 1 (PSGL-1) ligand coupled onto human red blood cells (RBCs). The data indicated that L-selectin shedding reduced the amount of membrane-anchored L-selectin and lowered both its reverse and forward rates. These results suggested that the rolling dynamics of activated leukocytes was determined by two opposite impacts: reducing the surface presentation would enhance the rolling but lowering the kinetic rates would decrease the rolling. This finding provides a new insight into under-standing how L-selectin shedding regulates leukocyte rolling and adhesion.

关键词：结合动力学选择素配体白细胞介素8 Jurkat细胞诱导细胞表面微管吸吮

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Dynamics of morphological changes for mitochondrial fission and fusion

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Science China(Physics,mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3.& LONG Mian 1,2 1 Key Laboratory of Microgravity (National Microgravity Laboratory), Beijing 100190, China 2 center for biomechanics and bioengineering, institute of mechanics, chinese academy of sciences, Beijing 100190, China 3.State Key Laboratory of Biomembrane and Membrane Biotechnology,{3. of Zoology, chinese academy of sciences, Beijing 100101, China 4{3. of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, Beijing 100044, China 1. Key Laboratory of Microgravity (National Microgravity Laboratory) Beijing 100190 China2. Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100190 China4. Institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing 100044 China3. State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

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Developing a microfluidic-based system to quantify cell capture efficiency

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Science China(Life sciences) 2009年第2期52卷 173-181页

作者： YANG Fan, GAO YuXin, ZHANG Yan, CHEN Juan & LONG Mian National Microgravity Laboratory and center for biomechanics and bioengineering,{3., chinese academy of sciences, Beijing 100080, China 1. National Microgravity Laboratory and center for biomechanics and bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing 100080 China

Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microflu- idic-based assay, however, remains to be further tested. In the current work, we developed a microflu- idic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quan- tified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses.

关键词： cell surface marker microfluidic capture efficiency

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Low Spring Constant Regulates P-selectin-PSGL-1 Bond Rupture

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生物物理学报 2009年第S1期25卷 91-92页

作者： Yan Zhang, Ganyun Sun, Shouqin Lü, Ning Li, and Mian Long Key Laboratory of Microgravity(National Microgravity Laboratory) and center for biomechanics and bioengineering,{3., chinese academy of sciences, Beijing 100190, China

Forced dissociation of selectin-ligand bonds is crucial to such biological processes as leukocyte recruitment, thrombosis formation, as well as tumor metastasis. While

关键词： Selectin-ligand bonds Rupture Spring constant Optical trap

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Cell adhesion mediated by β_2 integrin-ICAM-1 interactions

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医用生物力学 2010年第S1期25卷 14-15页

作者： Changliang Fu,Dongying Zhan,Chunfang Tong,Manliu Wang,Shouqin Lü,Yan Zhang,Mian Long(1 Key Laboratory of Microgravity,institute of mechanics,chinese{3. of sciences,2 National Microgravity Laboratory and 3.center of biomechanics,and bioengineering,institute of mechanics,chinese{3. of sciences,Beijing 100190,China.)

Introduction During the inflammatory responses,leukocytes are recruited at the site of high endothelial venule,roll over the endothelium surface,form the firm adhesion to spread out,and finally transmigrate across the... 详细信息

关键词： ICAM PMN

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