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Organic Solvents Mediate Self-assembly of GAV-9 Peptide on Mica Surface

Acta biochimica et biophysica Sinica 2007年第4期39卷 285-289页

作者： Hai LI~(1,3) Feng ZHANG~1 Yi ZHANG~1* Jianhua HE~1 Jun HU~(1,2) 1 shanghai Institute of Applied Physics,Chinese Academy of sciences,shanghai 201800,china 2 bio-x life sciences research center.college of life science and biotechnology,shanghai jiaotong university,shanghai 200030,china 3 Graduate School of the Chinese Academy of sciences,Beijing 100049,china shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800 China Graduate School of the Chinese Academy of sciencesBeijing 100049 China bio-x life sciences research center College of Life Science and Biotechnology Shanghai Jiaotong UniversityShanghai 200030 China

Self-assembly of peptides into fibrils and other morphologies has attracted much attention inmany fields,especially in nanofabrication,pathology and *** this paper,self-assembly of GAV-9peptide in organic solvents,ethanol and acetone,was investigated using atomic force microscopy(AFM)and attenuated total reflectance-Fourier transform infrared spectroscopy(ATR-FTIR).The results indicatedthat GAV-9 self-assembled into various nanostructures in both solvents after deposited and evaporated *** with β-sheet conformation were observed in both solvents when the peptide concentrationwas higher than 280 μ***,ordered fibrils with β-sheet conformation were formed in ethanol,butnot in acetone,with a peptide concentration ranging from 7 μM to 28 μ*** attribute the formation ofvarious nanostructures to the different physicochemical properties of the polar organic solvents on the self-assembly of GAV-9 peptide.

关键词： self-assembly GAV-9 peptide organic solvents ATR-FTIR AFM

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cvhA Gene of Streptomyces hygroscopicus 10-22 Encodes a Negative Regulator for Mycelia Development

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Acta biochimica et biophysica Sinica 2006年第4期38卷 271-280页

作者： Heng-An WANG~(1,2#) Lei QIN~(2#) Ping LU~(1*) Zhi-xuan PANG~2 Zi-xin DENG~(3*) Guo-Ping ZHAO~(2*) 1 biotechnology Institute,School of Agriculture and biology,shanghai jiaotong university,shanghai 201101,china 2 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institute for biological sciences,Chinese Academy of sciences,shanghai 200032,china 3 bio-x life science research center,shanghai jiaotong university,shanghai 200030,china. biotechnology Institute School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 201101 China Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Chinese Academy of SciencesShanghai 200032 China bio-x life science research center Shanghai Jiaotong UniversityShanghai 200030 China.

A five-gene cluster cvhABCDE was identified from Streptomyces hygroscopicus *** thefirst gene of this cluster,cvhA encoded a putative sensor histidine kinase with a predicted sensor domainconsisting of two trans-membrane segments at the N-terminus and a conserved HATPasecdomain at the *** C-terminus polypeptide of CvhA expressed in Escherichia coli was purified and shown to beautophosphorylated with [γ-32P]ATP in *** phosphoryl group was acid-labile and basic-stable,whichsupported histidine as the phosphorylation *** obvious difference of mycelia development was ob-served between the null mutant of cvhA generated by targeted gene replacement and the wild-type parentalstrain 10-22 grown on solid soya flour medium with 2%-8% glucose or sucrose,but the cvhA mutant couldform much more abundant aerial mycelia and spores than the wild-type strain on solid soya flour mediumsupplemented with 6%-8% mannitol,6%-8% sorbitol,4%-6% mannose,or 4%-6% *** pheno-type was complemented by the cloned wild-type cvhA gene,and no difference was observed for growthcurves of the cvhA mutant and the wild strain in liquid minimal medium with the tested sugars at a concen-tration of 4%,6% and 8%.We thus propose that CvhA is likely a sensor histidine kinase and negativelyregulates the morphological differentiation in a sugar-dependent manner in *** 10-22.

关键词： sensor histidine kinase aerial mycelia sugar metabolism

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高效液相色谱法同时检测南昌霉素和梅岭霉素

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色谱 2002年第1期20卷 43-45页

作者：孙宇晖周秀芬涂国全邓子新上海交通大学生命科学技术学院biox生命科学研究中心上海200030 江西农业大学生物工程系生物技术研究开发中心江西南昌330045

采用高效液相色谱法同时分离测定聚醚类抗生素南昌霉素和十六元大环内酯类抗生素梅岭霉素。色谱柱为WatersxTerraTM RP18柱 (3 9mmi.d .× 15 0mm ,5 μm) ,流动相为乙腈水溶液 ,流速为 0 8mL/min ,其梯度洗脱程序为 5 7% (体积分数 ,... 详细信息

采用高效液相色谱法同时分离测定聚醚类抗生素南昌霉素和十六元大环内酯类抗生素梅岭霉素。色谱柱为WatersxTerraTM RP18柱 (3 9mmi.d .× 15 0mm ,5 μm) ,流动相为乙腈水溶液 ,流速为 0 8mL/min ,其梯度洗脱程序为 5 7% (体积分数 ,下同 )乙腈 (30min)→ 70 %乙腈 (2min)→ 80 %乙腈 (2min)→ 90 %乙腈 (16min)。检测波长为 2 34nm ,柱温 2 5℃。该法测定的灵敏度好 ,加标回收率高 ,可用于南昌链霉菌发酵液中南昌霉素和梅岭霉素的定量测定。此法具有灵敏、准确、快速的特点。

关键词：高效液相色谱法南昌霉素梅岭霉素分析抗生素南昌链霉菌发酵液

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The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice

The AGL6-like gene OsMADS6 regulates floral organ and merist...

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上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： Haifeng Li1*, Wanqi Liang1*, Ruidong Jia2*, Changsong Yin1, Jie Zong1, Hongzhi Kong2, Dabing Zhang1 1School of life science and biotechnology, shanghai jiaotong university, shanghai 200240, china 2Institute of Botany, Chinese Academy of sciences, Beijing 100093, china 3bio-x research center, Key Laboratory of Genetics & Development and Neuropsychiatric Diseases, Ministry of Education, shanghai jiaotong university,shanghai 200240, china

Although AGAMOUS-LIKE6 (AGL6) MADS-box genes are ancient with wide distribution in gymnosperms and angisperms, their functions remain less understood. Here we show the

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The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development

The SEPALLATA-like gene OsMADS34 is required for rice inflor...

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上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： xingchun Gao1*, Wanqi Liang1*, Changsong Yin1, Shenmin Ji1, Hongmei Wang1, xiao Su1, Chunce Guo2, Hongzhi Kong2, Hongwei xue3, Dabing Zhang1, 4 1School of life science and biotechnology, shanghai jiaotong university, shanghai 200240 2Institute of Botany, the Chinese Academy of sciences, Beijing 100093 3National Key Laboratory of Plant Molecular Genetics, shanghai Institute of Plant Physiology and Ecology, shanghai Institutes for biological sciences, Chinese Academy of sciences, shanghai 200032 4bio-x research center, Key Laboratory of Genetics & Development and Neuropsychiatric Diseases, Ministry of Education, shanghai jiaotong university, shanghai 200240

Grass plants evolve distinct inflorescences and spikelets that determine grain yields. However, the mechanisms underlying the specification of inflorescences and spikelets in grasses remain

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