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离体培养马铃薯茎顶端(或腋芽)生长点的初步研究

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Journal of Genetics and Genomics 1976年第1期 51-56+96-97页

作者： Laboratory of plant cell and tissue culture, 305 Eesearch Group, Institute of Genetics, Academia Sinica 中国科学院遗传研究所组织培养室、三室五组

马铃薯是一种高产作物,它蕴藏着很大的增产潜力。由于病毒危害的增加,严重的影响马铃薯的生产和栽培。近年来国外许多科学工作者应用离体培养茎顶端(或腋芽)生长点的方法来获得无病毒的马铃薯植株。试验证明这种无病毒植株,在性状上无... 详细信息

马铃薯是一种高产作物,它蕴藏着很大的增产潜力。由于病毒危害的增加,严重的影响马铃薯的生产和栽培。近年来国外许多科学工作者应用离体培养茎顶端(或腋芽)生长点的方法来获得无病毒的马铃薯植株。试验证明这种无病毒植株,在性状上无变异且生育良好,有明显的增产效果。作为良种繁育是生产原原种的有效措施。这一方法不仅具有实践价值,而且在遗传育种理论上也具有一定的意义。为了落实毛主席关于“备战、备荒、为人民”,“深挖洞、广积粮、不称霸”的战略方针和提高马铃薯生产发展的需要,我们于1974年开始进行了这方面的研究工作。现将第一阶段试验中所获得的从茎顶端(或腋芽)生长点发育成植株及植株形成块茎的初步结果报道如下。

关键词：生长点培养块茎地下茎赤霉素腋芽侧芽离体培养马铃薯茎

Survival of Human Metallothionein-2 Transplastomic Chlamydomonas reinhardtii to Ultraviolet B Exposure

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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 187-193页

作者： Yu-Kun ZHANG~1 Gui-Fang SHEN~2 Bing-Gen RU~1*1 National Laboratory of Protein Engineering and plant Genetic Engineering,Peking university,Beijing 100871,China 2 Biotechnology Research Institute,the Chinese Academy of agric.ltural Sciences,Beijing 100081,China National Laboratory of Protein Engineering and plant Genetic Engineering Peking UniversityBeijing 100871 China Biotechnology Research Institute the Chinese Academy of Agricultural SciencesBeijing 100081 China

Solar ultraviolet (UV) radiation has a great influence on green organisms,especially planktonlike Chlamydomonas.A human metallothionein-2 gene,which is generally considered to have an anti-radiationfunction by its coding product,was transferred into the chloroplast genome of Chlamydomonas *** dynamically measure the UV effects on Chlamydomonas cells grown in liquid tris-acetate-phosphatemedium,a new method was developed based on the relationship between the chlorophyll content of an algalculture and its absorbance at 570 nm after the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium *** this experiment,both the wild-type and the transplastomic *** ceils were cultivated in 96-well microplates containing liquid tris-acetate-phosphate medium in the absence or presence of zinc,copper,cadmium and *** transgenic *** showed a higher resistance than wild-type to UV-Bexposure under all the examined *** in the medium had positive impacts on both types of cells,but had significant influence only on the transplastomic ***,the high cell viability of the trans-genie alga at the end of the 8 h UV-B treatment disappeared after a 20-h recovery *** did notprotect cells from UV-B damage,but clearly enhanced the growth of both wild-type and transgenic ***.

关键词： ultraviolet B MTT transplastomic Chlamydomonas metallothionein

Growth phase-dependent expression of proteins with decreased plant-specific N-glycans and immunogenicity in tobacco BY2 cells

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Science China(Life Sciences) 2009年第8期52卷 739-746页

作者： YIN BoJiao1,2,ZHENG NuoYan1,2,LI Yin1,TANG SanYuan2,LIANG LiMing1 & XIE Qi1,2 1 State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen(Zhongshan) university,Guangzhou 510275,China 2 State Key Laboratory of plant Genomics,National Centre for plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen (Zhongshan) University Guangzhou China State Key Laboratory of plant Genomics National Centre for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China

plants possess some desirable characteristics to synthesize recombinant glycoproteins for pharma-ceutical ***,the mammalian glycoproteins produced in plants are somewhat different from their natural counterparts in terms of *** immunogenicity of plant-specific glyco-epitopes is the major concern in human ***,the distribution of N-glycans in different growth phases of tobacco BY2 cells and their immunogenicity in mice were *** was ob-served that the percentage of β1,2-xylose and α1,3-fucose in proteins of growing cells increased and the corresponding protein extracts caused accelerated immune response in *** on this observation,the recombinant erythropoietin in BY2 cells was expressed and characterized,and Western blot analysis showed that the recombinant erythropoietin contained a relatively small amount of plant-specific glyco-epitopes in the early phase of culture *** study may provide a simple but effective strategy for the production of therapeutic glycoproteins with human-like N-glycan structures in plant hosts to avoid a great allergenic risk.

关键词： BY2 cell,N-glycans,protein expression immunogenicity

Ability of Ectomycorrhizal Fungns Laccaria bicolor S238Nto Increase the Growth of Donglas Fir Seedlings andTheir Phosphorns and Potassinm Uptake

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Pedosphere 1994年第3期4卷 217-224页

作者： HUANG JIANGUO and F. LAPEYRIE(Dept. of Soil Science, Southwest agric. Univ. Chongqzig 630716(China))INRA. Microbiologie, Center de Nancy, Champenoux 54280 (France)

Ectomycorrhizal fungus Laccaria bicolor S238N, isolated from a forest soil in central France in 1990s,has demonstrated unequivocally an ability to promote pine growth. In the present nursery bed experiment,the ability of this ectomycorrhizal fungus to increase growth and P and K uptake of Douglas Fir seedlings（Zone 22） was examined. Growth of inoculated seedlings was over twice （plant height） and three times（biomass） that of non-inoculated ones. Similarly, both the concentrations and the amounts of P and Kuptake by seedlings were significantly increased by fungal inoculation, indicating the improvement of P and Knutrition in mycorrhizal seedlings. In contrast, Al-p in the soils was decreased obviously by plants, especiallyby mycorrhizas, suggesting utilization of this soil P pool by plants and more efficient Al-P mobilization bymycorrhizas than by nonmycorrhizas. Moreover, K extracted by 1 mol/L HCl following consecutive extractionof H2O and CH3COONH4, which may not be plant available, could be utilized by fungus colonized *** could be explained by the release of protons and oxalate by hypae which leads to replacement of interlayerK in nonexpanded 2:1 clay minerals and bio-weathering of phyllosilicates.

关键词： Douglas Fir ectomycorrhizal fungi K P

Release of active oxygen from phytopathogenic bacteria and their regulation

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Chinese Science Bulletin 1999年第1期44卷 71-75页

作者： LI Hongyu WANG JinshengKey Laboratory of Monitoring and Management of plant Diseases and Pests, Ministry of Agriculture Department of plant Protection, Nanjing Agriculture university, Nanjing 210095, China Corresponding author Key Laboratory of Monitoring and Management of plant Diseases and Pests Ministry of Agriculture Nanjing China Department of plant Protection Nanjing Agriculture University Nanjing China

Some phytopathogenic bacteria including Xanthomonas oryzae pv. oryzae （a rice leaf bacterial blight pathogen） and its virulent gene mutant XooM3105,Xanthomonas campestris pv. campestris, Erwinia carotovora subsp, carotovora and so on, could release active oxygen such as O2 and H2O2 by ***2+, NADPH and O2 concentration regulated the response. The response is sensitive to heat and proteinase. These results show that the component releasing active oxygen in the bacterial plant pathogen may involve special protein and enzyme and be secreted to culture liquid. Various components of rice tissue can significantly induce the release of active oxygen in Xanthomonas oryzae pv. oryzae.

关键词： phytopathogenic bacteria release of active oxygen rice bacterial leaf blight pathogen interaction between host and pathogen signal transduction.

CHEMICAL STRESS-INDUCED EFFECTS ON OCTOPAMINE IN COCKROACH, BLATTELA GERMANICA L

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CHEMICAL STRESS-INDUCED EFFECTS ON OCTOPAMINE IN COCKROACH, ...

The ⅩⅤth International plant Protection Congress

作者： PAN Can-ping LI Wei-xi and JIANG Shu-ren Department of Applied Chemistry China agric. university Beijing 100094 China

来源： cnki会议评论

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小麦叶锈菌生理分化研究中的问题和改进意见

河北农业大学学报 1992年第3期15卷 91-94页

作者： Wheat Leaf Rust Research Group, Hebei agric. Univ. , Baoding, China 河北农业大学小麦叶锈病研究组

Stakman建立的用鉴别寄主鉴定锈菌生理小种的方法对抗病育种曾起了很大的作用,然而实践证明还存在不少问题。(1)生物间遗传学指出,Stakman建立的方法。其观点偏重于锈菌分类,而忽视寄主和病原物的相互作用。所以所用的鉴别寄主仅注意其... 详细信息

Stakman建立的用鉴别寄主鉴定锈菌生理小种的方法对抗病育种曾起了很大的作用,然而实践证明还存在不少问题。(1)生物间遗传学指出,Stakman建立的方法。其观点偏重于锈菌分类,而忽视寄主和病原物的相互作用。所以所用的鉴别寄主仅注意其鉴别作用,可与生产毫无关系,可以鉴定出许多小种,而不知道它们与生产有关的品种有什么关系。(2)在自然间寄主和病原物是以群体对群体起相互作用的,而传统研究是研究个体与个体的关系。此外,小种内存在变异性,被鉴定为同一小种的不同菌株的毒性不一定相同,任意选择一个菌株来测定品种的抗病性未必有代表性,用这种方法测出的抗病品种推广出去就容易引起抗病性丧失问题。(3)在不同生态条件下形成的锈菌的群体毒性不一定相同,这种毒性不同的类群我们称为生态类群。过去的小麦品种抗病性测定工作多在局限的条件下用人工接种进行。把用这种方法测出的抗病品种推广到广大的锈菌群体中去,也容易引起抗病性丧失问题。我们赞成今后研究小麦锈病应有群体观点,用毒力频率,毒力组合,抗性组合等方法来研究小麦群体和锈病群体的关系。应有生态观点,用异地鉴定来监测锈菌毒性和品种抗性的变异。应有进化观点,以基因鉴定来代替小种鉴定,把寄主的病原物相互作用研究放在遗传学基础上。在利用已知基因作鉴别寄主方面,虽然抗原的抗性基因可与Lr近等基因系的相同,但由于表现型相同基因型不一定相同,所以这两种材料都得同时采用,不能互相代替。抗性基因鉴定是复杂的。一般认为阿芙乐尔会有Lr26基因。Bastos认为除Lr26外,还有Lr3。杨文香认为除Lr26和Lr3外,还有Lrl4a。又Bastos认为洛夫林13含有Lr26和Lr3,相文香认为只含有Lr24,检查不到Lr3。

关键词：小麦叶锈菌 Lr近等基因系抗病性

plant regeneration from protoplasts of hydroxyprolineresistant cell line in Onobrychis viciaefolia

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cell Research 1995年第2期5卷 187-194页

作者： XU ZIQIN JINGFEN JIA(cell Blology Laboratory, Lanzhou university, Lanzhou 730000, China.) cell Biology Laboratory Lanzhou University Lanzhou China

An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were *** number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.

关键词： Sainfoin (Onobrychis Viciaefolia) hydroxyproline resistant cell line protoplast culture plant regeneration

Involvement of NADPH oxidase NtrbohD in the rapid production of H2O2 induced by ABA in cultured tobacco cell line BY-2

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Progress in Natural Science:Materials International 2008年第3期18卷 267-271页

作者： Fushun Hao a,b, Jinguang Zhang a, Zhonglian Yu a, Jia Chen a, a State Key Laboratory of plant Physiology and Biochemistry, College of Biological Science, China agric.ltural university, Beijing 100094, China b Henan Key Laboratory of plant Stress Biology, College of Life Science, Henan university, Kaifeng 475004, China State Key Laboratory of plant Physiology and Biochemistry College of Biological Science China Agricultural University Beijing 100094 China Henan Key Laboratory of plant Stress Biology College of Life Science Henan University Kaifeng 475004 China

The mechanisms for the production of hydrogen peroxide (H2O2) induced by abscisic acid (ABA) were investigated in suspension culture cells of tobacco BY-2 cells. The results showed that the immediate generation of H2O2, which was mainly derived from super- oxide dismutase-catalyzed dismutation of superoxide radical, was significantly induced by ABA. Furthermore, treatment of the cultured tobacco cells with ABA resulted in a time-dependent quick increase in plasma membrane (PM) NADPH oxidase activity, which coin- cided on time and magnitude with the elevation in ABA-induced accumulation of H2O2. Moreover, these enhanced effects were pro- nouncedly inhibited by two NADPH oxidase inhibitors, diphenylene iodonium and imidazole, suggesting that PM NADPH oxidase is involved in the rapid accumulation of H2O2 in cultured tobacco cells. In addition, analysis of the expression level of NtrbohD, a PM NADPH oxidase gene in tobacco, by RT-PCR and protein gel blot revealed that the gene at both mRNA and protein levels was upregulated by ABA, indicating that NtrbohD participates in the ABA-stimulated rapid production of H2O2 in tobacco culture cells. Taken together, these findings suggest that ABA induces the rapid accumulation of reactive oxygen species via NADPH oxidase in sus-pension culture cells of tobacco, and that NADPH oxidase and H2O2 appear to be important components in ABA signal transduction pathway in plants.

关键词： ABA cultured tobacco cells H2O2 NADPH oxidase NtrbohD