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Application of EGFP-EGF fusions to explore mechanism of endocytosis of epidermal growth factor

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Acta Pharmacologica Sinica 2007年第1期28卷 111-117页

作者： Hua JIANG~2 Jie ZHANG~(2,3) Bi-zhi SHI~(2,3) Yu-hong XU~3 Zong-hai LI~(2,3,4) Jian-ren GU~(2,3,4)2 State Key Laboratory of Oncogenes and Related Genes,shanghai cancer Institute,Medical college of fudan university 3 school of pharmacy,shanghai Jiaotong university,shanghai 200032,{6. StateKeyLaboratoryofOncogenesandRelatedGenes ShanghaiCancerInstituteMedicalCollegeofFudanUniversity schoolofpharmacy ShanghaiJiaotongUniversityShanghai200032China

Aim:To develop a simple method for monitoring protein localization of epidermalgrowth factor(EGF)in living ***:Enhanced green fluorescent protein(EGFP)was used as an autofluorescent tag to label EGF ***-PAGE andWestern blot analysis were used to detect the expression of the EGFP-tagged EGF(EGFP-EGF)*** cell-binding and internalization activity of EGFP-EGFwere analyzed by fluorescence-activated cell sorting(FAcS)and confocal ***:EGFP-EGF protein was expressed in Escherichia coli and purified.A cell-binding assay demonstrated that the EGFP-EGF protein could bind effi-ciently to the cells expressing *** binding and internalization of EGFP-EGFcan be visualized even at a very low concentration under confocal *** FAcS-based assay for internalization activity indicated the accumulation ofinternalized EGFP-EGF over ***,the results of the competitionassay indicated its EGFR binding *** such a method,it does notneed to label EGF with chemicals and avoid light in the experimental ***:The fusion protein EGFP-EGF has several characters including highsensitivity,stability and convenience for manipulation,and is a powerful tool forthe study of EGF endocytosis.

关键词： receptor epidermal growth factor endocytosis purification expression

A NONLOcAL NONLINEAR BOUNDARYVALUE PROBLEM FOR THE HEAT EQUATIONS

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chinese Annals of Mathematics,Series B 1996年第3期17卷 365-374页

作者： YAN JINHAI(Department of Mathematics, fudan university, shanghai 200433, c.ina) fudan UNIV DEPT MATHSHANGHAI 200433PEOPLES R CHINA

The existence and limit behaviour of the solution for a kind of nonlocal nonlinear boundaryvalue condition on a part of the boundary is studied for the heat equation, which physicallymeans that the potential is the function of the total flux. When this part of boundary shrinksto a point in a certain wayt this condition either results in a Dirac measure or simply disappearsin the corresponding problem.

关键词： Heat equation Nonlinear boundary problem Potential Limit behavior

多剂量包装中药配方颗粒调剂管理上海共识（2023）

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上海中医药大学学报 2024年第5期 1-9页

作者： clinical chinese Materia Medica Group of Hospital pharmacy Professional committee of shanghai Pharmaceutical Association Hospital pharmacy Branch of shanghai Association of Traditional chinese Medicine Writing Group of"shanghai consensus on Dispensing Management of Multi-dose Packaged Traditional chinese Medicine Formula Granules (2023)" 上海市药学会医院药学专业委员会临床中药学组上海市中医药学会医院药学分会《多剂量包装中药配方颗粒调剂管理上海共识（2023）》编写组

多剂量包装中药配方颗粒利用设备进行调剂，在上海各医疗机构使用广泛，但药品的调剂质量受多方面因素影响较大，管理要求亟待完善提高。为此，上海市药学会医院药学专业委员会临床中药学组等单位组织专家，根据有关政策法规要求，参考... 详细信息

多剂量包装中药配方颗粒利用设备进行调剂，在上海各医疗机构使用广泛，但药品的调剂质量受多方面因素影响较大，管理要求亟待完善提高。为此，上海市药学会医院药学专业委员会临床中药学组等单位组织专家，根据有关政策法规要求，参考相关标准规范和近5年的文献报道，结合上海市实际情况，围绕与多剂量包装中药配方颗粒调剂相关的人员要求、温湿度控制、设备要求、清洁污染、效期管理、发药交待等管理问题，以“陈述”形式形成上海地区多剂量包装中药配方颗粒调剂管理专家共识，以期进一步规范中药配方颗粒的临床调剂工作，加强应用的安全性、合理性和可控性。

关键词：中药配方颗粒多剂量包装调剂专家共识上海

The neural mechanisms of pain-related affect and memory

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Progress in Natural Science:Materials International 2006年第4期16卷 338-345页

作者： ZHANG Yuqiu Institute of Neurobiology, fudan university, shanghai 200433, c.ina [a]Institute of Neurobiology Fudan University Shanghai China

The pain experience includes a sensory-discriminative and an emotional-affective components. The affective dimension refers to the unpleasantness or aversion of sensation. The great progress at the genetic, molecular, cellular, and systemic levels on the study of the sensory dimension of pain has been made over past four decades. However, "to consider only the sensory features of pain, and ignore its motivational and affective properties, is to look at only part of the problem and not even the most important part of that". A line of clinic observations indicate that the patients with chronic pain suffer from much more affective disturbance than pain itself. Obviously, physiological arousal and hypervigilance to pain cause negative affect, such as anxiety, anger, worry, aversion, even tendency of suicide, these negative affective states in turn enhance pain sensation. Today, more and more attention has been paid to the study on mechanisms underlying affective dimension of pain. In order to deepen and expand our understanding of the nature of pain, this review summarizes the main progress and recent findings from our laboratory regarding affective component of pain in neuroanatomy, neurophysiology, and cell biochemistry.

关键词： pain emotion medial pain system anterior cingnlate cortex formalin conditioned place avoidance cAMP responsive element binding protein extracellular signal-regulated kinase.

Kinetics of the formation of N2O in the catalytic oxidation of NH3 on Pt-Rh filament

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Acta chimica Sinica(English Edition) 1985年第1期3卷 7-13页

作者： HU Zu-Shao QIN Qi-Zong Laser chemistry Laboratory,fudan university,shanghai Laser chemistry Laboratory Fudan University Shanghai

The kinetics of the reactions involving the formation of N2O in the catalytic oxidationof NH3 have been studied over polycrystalline Pt-10% Rh filament in a static system,using a Fou-rier transform infrared spectrometer to monitor the partial pressures of NH3 and *** soleformation of N2O proceeds over the filament temperatures range of 250～350℃.The reaction rateis of first order in NH3 pressure and half order in O2 pressure,and the rate equation can be writtenas v=kapp·PNH3·PO21/*** apparent activation energy of the N2O formation reaction fromthe temperature coefficient of the rate constants is found to be 25 kcal/*** interpretation ofthese results in terms of Eley-Rideal type mechanism has been *** rate-controllingstep might be the reaction between gaseous ammonia molecules and the chemisorped oxygen atomson the surface.

关键词： Kinetics of the formation of N2O in the catalytic oxidation of NH3 on Pt-Rh filament Pt Rh NH

Antisense Tiam1 Down-Regulates the Invasiveness of 95D cells in Vitro

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 537-540页

作者： Min HOU, Li TAN, Xia WANG, and Yun-Song ZHU Department of Molecular Genetics, shanghai Medical college, fudan university & Key Laboratory of Molecular Medicine, Ministry of National Education, shanghai 200032, c.ina Department of Molecular Genetics Shanghai Medical College Fudan University & Key Laboratory of Molecular Medicine Ministry of National Education

As a specific guanine nucleotide exchange factor of Rac1, Tiam1 (T-lymphoma invasion and metastasis inducing protein 1) is involved in a number of cellular events, such as cytoskeleton reorganization, cell adhesion, and cell migration. Since Tiam1 was implicated in the invasion and metastasis of T-lymphoma cells and breast tumor cells, we compared the expression level of Tiam1 in two human giant-cell lung carcinoma cell strains with high or low metastasis potential, and found that Tiam1 expression level in high-metastatic 95D cells was higher than that in low-metastatic 95c cells. To further confirm the role of Tiam1 in invasion and metastasis, we constructed the antisense Tiam1 expression plasmid (pcDNA3-anti- Tiam1), which was transfected into 95D cells. A stable transfected clone with decreased Tiam1 expression was screened and selected for further research. Transwell assay showed that down-regulation of endogenous Tiam1 by anti-Tiam1 can reduce the in vitro invasiveness of 95D cells. Our results suggested that Tiam1 signaling contributed to the invasion and metastasis of the human giant-cell lung carcinoma cells.

关键词： T-lymphoma invasion and metastasis inducing protein 1 (Tiam1) invasion and metastasis

A characterization of solvability for a class of networks

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Science c.ina(Information Sciences) 2012年第4期55卷 747-754页

作者： YUAN chen & KAN HaiBin shanghai Key Lab of Intelligent Information Processing,school of computer Science,fudan university,shanghai 200433,c.ina 1. shanghai Key Lab of Intelligent Information Processing School of Computer Science Fudan University Shanghai 200433 China

In this paper,we find that Property P can be generalized to characterize the solvability of a kind of networks with any number of sources,thus partially answering the open problem as to whether there are properties similar to Property P to characterize the solvability of some *** an application,for a given integer n,we construct such a solvable network that has no solvable solution if its alphabet size is less than n.

关键词： capacity coding capacity network coding solvability

catalytic hydrolysis of cFc-12 over solid acid Ti(SO_4)_2

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chinese chemical Letters 2000年第4期11卷 311-314页

作者： Zhen MA Wei Ming HUA Yi TANG Zi GAO (Department of chemistry. fudan university. shanghai 200433) Department of chemistry Fudan University Shanghai 200433 China

The catalytic hydrolysis of dichlorodifluoromethane (cFc-12) was investigated over solid acid Ti(SO_4)_2. The catalytic activity decreased with the calcination temperature. When space velocity was 6.1 h^(-1) g-cat^(-1). the cPc-12 conversion at 310c over Ti(SO_4)_2 calcined at 350c remained about 98.5% during 36. h on stream. and the selectivity to by-products remained zero. The findings enlarged the scope of traditional catalyst systems for the cFcs decomposition.

关键词： chlorofluorocarbons. catalytic decomposition. solid acid Ti(SO_4)_2.

The Mystery of Sleep:Molecular Mechanisms of Histaminergic Roles in Sleep-wake Regulation

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生物物理学报 2009年第S1期25卷 52-53页

作者： Zhi-Li Huang1,2,Wei-Min Qu2 1 State Key Laboratory of Medical Neurobiology 2 Department of Pharmacology, shanghai Medical college of fudan university, shanghai 200032, c.ina.

The histaminergic neurons are confined to the tuberomammillary nucleus (TMN) of the posterior hypothalamus, and send their fibers to almost all regions of the brain. A

关键词： TMN