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New development of biliary surgery in china

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World Journal of Gastroenterology 2000年第2期6卷 187-192页

作者： Zhi Qiang Huang The Research Institute of general Surgery,The general hospital of pla,beijing 100853,china 解放军总医院北京

CHARACTERISTICS OF BILIARY CALCULOUSDISEASES IN china:THE CHANGING SCOPEDiseases of the biliary tract in china is complicatedwith the prevalence of primary infection of the bileduct *** the middle of the 20th century,biliary infection,biliary parasitic infestation,andbiliary stones made up the three chief componentsof biliary diseases in *** to the

关键词： biliary surgery cholelithiasis intrahepatic stones carcinoma gallbladder carcinoma bile duct

Neural precursors derived from human embryonic stem cells

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Science china(Life Sciences) 2005年第3期48卷 295-299页

作者： Peng Hongmei 1,2 & Chen Gui’an 1 1. department of Obstetrics and Gynecology, Peking University Third hospital, beijing 100083, china 2. department of Obstetrics and Gynecology, general hospital of pla, beijing 100853, china 1. department of Obstetrics and Gynecology Peking University Third Hospital 100083 Beijing China 2. department of Obstetrics and Gynecology General Hospital of PLA 100853 Beijing China

Human embryonic stem (hES) cells provide a promising supply of specific cell types for transplantation therapy. We presented here the method to induce differentiation of purified neural precursors from hES cells. hES cells (Line PKU-1 and Line PKU-2) were cultured in sus- pension in bacteriological Petri dishes, which differentiated into cystic embryoid bodies (EBs). The EBs were then cultured in N2 medium containing bFGF in poly-L-lysine-coated tissue culture dishes for two weeks. The central, small cells with 2―3 short processes of the spreading out- growth were isolated mechanically and replated. The resulting neurospheres were cultured in suspension for 10 days, then dissociated into single cell suspension with a Pasteur pipette and plated. Cells grew vigorously in an attached way and were passed every 4―5 days. Almost all the cells were proved nestin positive by immunostaining. Following withdrawal of bFGF, they differentiated into neurons expressing β-tubulin isotypeIII, GABA, serotonin and synaptophysin. Through induction of PDGF-AA, they differentiated into astrocytes expressing GFAP and oli- godendrocytes expressing O4. The results showed that hES cells can differentiate into typical neural precursors expressing the specific marker nestin and capable of generating all three cell types of the central nervous system (CNS) in vitro.

关键词： human embryonic stem cell, differentiation, neural precursor.

Improvement of the classification accuracy in discriminating diabetic retinopathy by multifocal electroretinogram analysis

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Progress in Natural Science:Materials International 2007年第8期17卷 913-918页

作者： Xu Jin~1 Hu Guangshu~(1**) Huang Houbin~2 (1.department of Biomedical Engineering,Tsinghua University,beijing 100084,china 2.department of Ophthalmology,general hospital of Chinese pla,beijing 100853,china) [a]department of Biomedical Engineering Tsinghua University Beijing China [b]department of Ophthalmology General Hospital of Chinese PLA Beijing China

The multifocal electroretinogram (mfERG) is a newly developed electrophysiological *** this paper,a classifi- cation method is proposed for early diagnosis of the diabetic retinopathy using mfERG *** records were obtained from eyes of healthy individuals and patients with diabetes at different *** each mfERG record,103 local responses were *** value of each point on all the mfERG local responses was looked as one potential feature to classify the experimental *** sub- sets were selected from the feature space by comparing the inter-intra *** on the selected feature subset,Fisher's linear classi- fiers were *** the final classification decision of the record was made by voting all the classifiers' *** the method to classify all experimental subjects,very low error rates were *** crucial properties of the diabetic retinopathy classification method are also discussed.

关键词： diabetic retinopathy multifocal electroretinogram feature selection linear classifier

Effective suppression of fibronectin synthesis by retrovirus delivered shRNA in rat cardiac fibroblasts

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Science china(Life Sciences) 2004年第4期47卷 368-375页

作者： WU Di1, HONG Quan1, CHEN Xiangmei1, FU Bo1, LI Aipingi2, LIU Hang1, BAI Xueyuan1 & WANG Jianzhong1 1. department of Nephrology, general hospital of pla, beijing 100853, china 2. department of Cardiology, general hospital of pla, beijing 100853, china 1. department of Nephrology General Hospital of PLA 100853 Beijing China 2. department of Cardiology General Hospital of PLA 100853 Beijing China

Extracellular matrix overexpression is a common final pathway that leads to ventricular remodeling. Fibronectin plays a pivotal role in this progress. In the work presented here, we explored the possibility of direct inhibition of fibronectin synthesis in rat cardiac fibroblasts by small interference RNA (siRNA). We found that siRNA could successfully suppress the fibronectin overexpression stimulated by angiotensin II. To overcome the limitations of plasmid-based siRNA, we subcloned the H1 promoter into pLXIN, a commercially available retroviral vector. We found that H1 promoter worked very well to form the small hairpin RNA (shRNA) on the retroviral vector, and the fibronectin expression was dramatically down regulated by shRNA. We think the retroviral shRNA delivery system that we have constructed may have potential roles in treating ventricular remodeling. Keywords RNA interference (RNAi) - Angtiotensin II (ATII) - extracellular matrix - fibronectin

关键词： RNA interference (RNAi) Angtiotensin II (ATII) extracellular matrix fibronectin

Parameter estimation for whole-body kinetic model of FDG metabolism

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Progress in Natural Science:Materials International 2006年第11期16卷 1164-1170页

作者： CUI Yunfeng~1, BAI Jing~1**, CHEN Yingmao~2 and TIAN Jiahe~2(1. department of Biomedical Engineering, Tsinghua University, beijing 100084, china 2. department of Nuclear Medicine, general hospital of pla, beijing 100853, china) [a]department of Biomedical Engineering Tsinghua University Beijing China [b]department of Nuclear Medicine General Hospital of PLA Beijing China

Based on the radioactive tracer [ ~ 18 F]2-fluoro-2-deoxy-D-glucose （FDG）, positron emission tomography （PET）, and compartment model, the tracer kinetic study has become an important method to investigate the glucose metabolic kinetics in human body. In this work, the kinetic parameters of three-compartment and four-parameter model for the FDG metabolism in the tissues of myocardium, lung, liver, stomach, spleen, pancreas, and marrow were estimated through some dynamic FDG-PET experiments. Together with published brain and skeletal muscle parameters, a relatively complete whole-body model was presented. In the liver model, the dual blood supply from the hepatic artery and the portal vein to the liver was considered for parameter estimation, and the more accurate results were obtained using the dual-input rather than the single arterial-input. The established whole-body model provides the functional information of FDG metabolism in human body. It can be used to further investigate the glucose metabolism, and also be used for the simulation and visualization of FDG metabolic process in human body.

关键词： compartment model FDG PET parameter estimation.

Measures of bioavailable serum sex hormone levels in aging Chinese by protein chip

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Science china(Life Sciences) 2006年第3期49卷 286-292页

作者： ZHOU Yong, CHANG Shuying, MENG Xiaoluo, YU Huafeng, WANG Luning, HE Jinggui, ZHANG Baohe, ZHANG Juntian, GENG Meiyu & DU Guanhua Institute of Marine Drugs and Food, Ocean University of china, Qingdao 266003, china Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, beijing 100050, china general hospital of Air Force, pla, beijing 100036, china 304 hospital of pla, beijing 100037, china beijing Tongren hospital, beijing 100730, china general hospital of pla, beijing 100853, china Institute of Marine Drugs Food Ocean University of China Qingdao 266003 China Institute of Materia Medica Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100050 China general hospital of Air Force PLA Beijing 100036 China 304 hospital of pla Beijing 100037 China beijing Tongren hospital Beijing 100730 China general hospital of pla Beijing 100853 China

The purpose of this study was to develop a protein chip technique based on receptor binding assays to measure bioavailable serum sex hormone levels (BSSHL). 224 aging healthy Chinese were investigated to get the referenced values of BSSHL for the first time. In the assays recombined sex hormone receptor proteins were jointed to polysaccharide coated slides to make protein chip,; the dose-dependence curves of sex hormone on chip were prepared. The data showed that this method had good precision (CV>16%); accuracy (Bias>10%),; the sensitivity could reach 1 pmol/L. From the results, BSSHL of men; women declined with aging, but no significant differences were observed. The BSSHL of aging men were higher than those of women. The bioavailable serum;rogen level of men was 52–112 pmol/L, women’s was 3–70 pmol/L; the whole group was 41.9–81.4 pmol/L. The bioavailable serum estrogen level of men was 0.8–3.0 pmol/L, women’s was 1.2–2.5 pmol/L; the whole group was 0.6–2.64 pmol/L. Based on the assays, BSSHL measurement by protein chip can meet the needs of epidemiological studies in terms of speed, accuracy; sample volume required,; was helpful in quantitative assessment of aging people’s health.

关键词： receptor protein chip estrogen - rogen aging

Silencing phospholipid scramblase 1 expression by RNA interference in colorectal cancer and metastatic liver cancer

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 393-400页

作者： Wei Cui,Shi-Yong Li,Jun-Feng Du,Zi-Man Zhu and Ping An department of general Surgery,general Surgery Center of the pla,general hospital of beijing Military Command,beijing 100700,china department of Hepatobiliary Surgery,First Affiliated hospital,general hospital of the pla,beijing 100037,china [a] department of general Surgery General Surgery Center of the PLA General Hospital of Beijing Military Command Beijing 100700 China [b] department of Hepatobiliary Surgery First Affiliated Hospital General Hospital of the PLA Beijing 100037 China

BACKGROUND:Phospholipid scramblase 1(PLSCR1) not only participates in the transbilayer movement of phospholipids,but also plays a role in the pathogenesis and progression of *** present study aimed to evaluate the effect of silencing PLSCR1 expression by RNA interference in colorectal cancer(CRC) and metastatic liver ***:The expression of PLSCR1 in CRC and metastatic liver cancer samples was assessed by *** cultured cells with the highest expression were selected for subsequent *** designed three siRNA oligonucleotide segments targeted at *** transfection was *** biological behavior of the cells in proliferation,adhesion,migration and invasion was ***:PLSCR1 protein expression increased significantly in the majority of CRC and metastatic liver cancer samples compared with normal *** cells had the highest expression of *** siRNA-390 oligonucleotide segment had the best silencing *** transfection,Lovo cell proliferation was significantly inhibited compared with the controls in the MTT *** and fibronectin adhesion assays showed Lovo cell adhesion was also significantly *** the migration assay,the number of migrating cells in the PLSCR1 siRNA-390 group was 50±12,significantly lower than the number in the siRNA-N group(115±28) and in the control group(118±31).In an invasion test,the number of invading cells in the PLSCR1 siRNA-390 group was 60±18,significantly lower than that in the siRNA-N group(97±26) and the control group(103±24).CONCLUSIONS:PLSCR1 is overexpressed in CRC and metastatic liver *** of PLSCR1 by siRNA inhibits the proliferation,adhesion,migration and invasion of Lovo cells,which suggests that PLSCR1 contributes to the tumorigenesis and tumor progression of ***1 may be a potential gene therapy target for CRC and associated metastatic liver cancer.

关键词： phospholipid scramblase 1 colorectal cancer metastatic liver cancer RNA interference

Method for tumor recognition with short dynamic PET images:Theory and simulation study

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Progress in Natural Science:Materials International 2009年第1期19卷 73-77页

作者： Huiting Qiao a,Jing Bai a,Yingmao Chen b,Jiahe Tianb a department of Biomedical Engineering,School of Medicine,Tsinghua University,beijing 100084,china b department of Nuclear Medicine,general hospital of pla,beijing 100853,china [a]department of Biomedical Engineering School of Medicine Tsinghua University Beijing 100084 China [b]Department of Nuclear Medicine General Hospital of PLA Beijing 100853 China

A new method was provided in this paper to recognize tumor in PET images automatically and reduce the waiting time of *** on the unsupervised clustering algorithm(ISODATA),this method diagnoses tumor with short dynamic PET *** theoretical basis of this method is that the metabolic characteristics of different tissues,represented by time-activity curve in the region of interest,are *** computer program was developed and validated using simulated dynamic PET data with small tumor in *** study shows that this method could recognize tumor with the short dynamic PET data in 10 min successfully,and this method was not sensitive to initial cluster center.

关键词： Dynamic PET Clustering algorithm Tumor recognition Simulation

Endogenous leptin fluctuates in hepatic ischemia/reperfusion injury and represents a potential therapeutic target

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World Journal of Gastroenterology 2010年第43期16卷 5424-5434页

作者： Ji Lin, Guang-Tao Yan, Hui Xue, Xiu-Hua Hao, Lu-Huan Wang, Research Laboratory of Biochemistry, Basic Medical Institute, Chinese pla general hospital, 28 Fuxing Road, beijing 100853, china Xiao-Ning Gao, department of Hematology, Clinical Division of Internal Medicine, Chinese pla general hospital, 28 Fuxing Road, beijing 100853, china Research Laboratory of Biochemistry Basic Medical Institute Chinese PLA General Hospital 28 Fuxing Road Beijing 100853 China. yan301@***

AIM: To evaluate the role of leptin in the internal disorders during hepatic ischemia/reperfusion injury. METHODS: A rat model of 70% hepatic ischemia/reperfusion injury was established, with groups of shamoperation (Sham), 60 min ischemia/60 min reperfusion (I60'R60'), I60'R150', I60'R240' and I60'R360'. Serum leptin was detected by a self-produced radioimmunoassay; serum glucose, total anti-oxidation capacity, myeloperoxidase, alanine transaminase and diamine oxidase were determined by relevant kits, while histologicalalterations and protein levels of leptin in the lung, liver and duodenum were examined by hematoxylin-eosin staining and immunohistochemistry. Spearman's rank correlation between leptin and other variables or grading of tissue impairment were analyzed simultaneously. RESULTS: Serum leptin in I60'R360' was significantly higher than in Sham and I60'R240' groups (both P 150' (both P 150' groups (both P 1). Serum myeloperoxidase in groups of I60'R240' and I60'R360' were lower than in I60'R150'group (both P 1), decreasing in the order of I60'R150', I60' R60', I60'R360' and I60'R240'; the levels in the liver in I60'R60' and I60'R240' were higher than in the Sham group (both P 1), while the levels in I60'R240' and I60'R360' were lower than in I60'R60' (both P 1); the levels in

关键词： Leptin Reperfusion injury Liver Lung Duodenum Recovery of function