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Characterization of Amphidinium(Amphidiniales,Dinophyceae)species from the china Sea based on morphological,molecular,and pigment data

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Journal of Oceanology and limnology 2022年第3期40卷 1191-1219页

作者： Zhaohe luo Hua ZHANG qun {2. Lei WANG Hala F MOHAMED Songhui LÜ Haifeng GU Third Institute of Oceanography Ministry of Natural ResourcesXiamen 361005China Research Center of Harmful Algae and Marine Biology College of Life Science and TechnologyJinan UniversityGuangzhou 510362China Shenzhen Academy of Environmental Science Shenzhen 510008China Botany&Microbiology Department Faculty of ScienceAl-Azhar University(Girls Branch)Cairo 11751Egypt Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519000China Observation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf Ministry of Natural ResourcesBeihai 536015China

Amphidinium species are amongst the most abundant benthic dinoflagellates in marine intertidal sandy *** of them are able to produce a variety of bioactive compounds that can have both harmful effects and pharmaceutical *** diversity of Amphidinium in shallow waters along the chinese coast was investigated by isolating single cells from sand,coral,and macroalgal samples collected from 2.12.to *** morphologies were subjected to examination using light microscopy(LM)and scanning electron microscopy(SEM).A total of 74 Amphidinium strains were morphologically identified,belonging to 11 species:***,***,***,***,***,***,***,***,***,***,***,as well as an undefined *** last seven species have not been previously reported in chinese *** carterae subcladesⅠ,Ⅱ,andⅣwere found in the South china Sea,while subcladeⅢwas only found in the Yellow *** body scales were observed on the surface of subcladesⅢ and Ⅳ,supporting the idea that *** might contain several different *** subunit ribosomal RNA(LSU rRNA)sequencesbased phylogeny revealed two groups(GroupsⅠandⅡ)within Amphidinium,which is consistent with the relative position of sulcus(in touch with cingulum or not).In addition,large differences in morphology and molecular phylogeny between ***(the type species of Amphidinium)and other species,suggest that a subdivision of Amphidinium might be *** pigment profiles of all available strains were analyzed by high performance liquid chromatography(HPLC).Eleven pigments,including peridinin,diadinoxanthin,diatoxanthin,pheophorbide(and pheophorbide a),antheraxanthin,β-carotene,and four different chlorophylls were *** high pheophorbide/pheophorbide a ratio in Amphidinium implies that it may be a good candidate as a natural source of photosensitizers,a well-known anticancer drug.

关键词： harmful algae benthic-epiphytic dinofl agellate phylogeny geographic distribution pheophorbide photosensitizers

Ultrastructure Characteristics of Different chinese Medicine Syndromes of Helicobacter pylori-Correlated Gastric Diseases

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chinese Journal of Integrative Medicine 2019年第12期25卷 917-921页

作者： HU ling li He-yuan CHEN Wan-{2. LAO Shao-xian luo Qi Institute of Gastroenterology Guangzhou University of Chinese MedicineGuangzhou(510405)China Department of Gastroenterology Chongqing Hospital of Traditional Chinese MedicineChongqing(400037)China

Objective: To explore the ultrastructure characteristics of patients with dampness-heat of Pi(Spleen)-Wei(Stomach) syndrome(DHPW) and Pi-qi deficiency syndrome(PQD), both of which are Helicobacter pylori(Hp)-correlated gastric diseases(HPCG), and implicate a helpful hint for the clinical microcosmic syndrome differentiation. Methods: Fourteen gastric mucosa samples from 6 chronic gastritis(CG) and 6 active peptic ulcer(including 8 DHPW, 4 PQD) as well as 2.healthy volunteers were collected and tested for Hp infection. The ultrastructure of gastric mucosa was observed under the transmission electron microscope(TEM). Results: Among 14 gastric mucosa samples, 8 of them were Hp positive(6 DHPW and 2.PQD), which were all accordance with the results screened by supermicro-pathological method. Under TEM, the normal gastric mucosa, with tidy microvilli and abundant in mucus granules, mitochondria and rough endoplasmic reticulum distributed evenly, and with smooth nucleus membrane. But in those specimens of DHPW with Hp infection, microvilli were presented with burr shape. Especially, those samples from dampnessheat syndrome with predominant heat type(DHSH) patients were more obvious, with microvilli damaged, mitochondria concentrated and distributed in disorder, secretory tubule extended. In dampness-heat syndrome with predominant dampness type(DHSD) patients, mucus granules aggregated obviously, mitochondria swelled and blurred, and rough endoplasmic reticulum crowded. For 2.samples of DHPW without Hp infection, their microvilli were intact, with mitochondria increased and gathered but well-distributed, and secretory tubule extended mildly. In 2.PQD patients with Hp positive, the specimens of microvilli were sparse, and their mucus granules and mitochondria were decreased, with fractured crests and vacuole, secretory tubules extension to nucleus membrane, and rough endoplasmic reticulum extension in a pool-like way, and nucleus condensed. The 2.samples from PQD patients wi

关键词： Helicobacter pylori-correlated gastric diseases supremicro-pathology dampness-heat syndrome of Pi(Spleen) and Wei(Stomach) Pi-qi deficiency chinese medicine

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大跨钢结构施工过程的数值跟踪和图形模拟

同济大学学报（自然科学版） 2004年第10期32卷 1295-1299页

作者：张其林罗晓群高振锋李子旭同济大学建筑工程系上海200092 上海建工(集团)总公司技术中心上海200050

建立了大跨悬臂预应力钢结构施工过程跟踪的数值分析模型 ,提出了在施工过程数值跟踪中对新增杆件节点定位、索段伸缩等问题的处理方法 ,介绍了施工过程数值跟踪的图形化模拟途径和技术 .经与实际工程现场测试结果比较。

关键词：钢结构施工过程数值跟踪图形模拟

Removal of Nitrogen, Phosphorus, and Organic Pollutants From Water Using Seeding Type Immobilized Microorganisms

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Biomedical and Environmental Sciences 2008年第2期21卷 150-156页

作者： liN WANG li-JING HUANG {2.-JIA YUN FEI TANG JING-HUI ZHAO YAN-qun liU XIN ZENG QI-FANG {2. Institute of Environmental Medicine School of Public Health Tongji Medical College Huazhong University of Science ＆ Technology MOE Key Laboratory of Environment and Health Wuhan 430030 Hubei China

Objective To study the possibility of removing nitrogen, phosphorus, and organic pollutants using seeding type immobilized microorganisms. Methods Lakes P and M in Wuhan were chosen as the objects to study the removal of nitrogen, phosphorus, and organic pollutants with the seeding type immobilized microorganisms. Correlations between the quantity of heterotrophic bacteria and the total nitrogen （TN）, total phosphorus （TP）, and total organic carbon （TOC） in the two lakes were studied. The dominant bacteria were detected, inoculated to the sludge and acclimated by increasing nitrogen, phosphorus and decreasing carbon source in an intermittent, time-controlled and fixed-quantity way. The bacteria were then used to prepare the seeding type immobilized microorganisms, selecting diatomite as the adsorbent cartier. The ability and influence factors of removing nitrogen, phosphorus, and organic pollutant from water samples by the seeding type immobilized microorganisms were studied. Results The coefficients of the heterotrophic bacterial quantity correlated with TOC, TP, and TN were 0.9143, 0.82.9, 0.7954 in Lake P and 0.9168, 0.7187, 0.602. in Lake M. Ten strains of dominant heterotrophic bacteria belonging to Pseudomonas, Coccus, Aeromonas, Bacillus, and Enterobateriaceae, separately, were isolated. The appropriate conditions for the seeding type immobilized microorgansims in purifying the water sample were exposure time=2. h, pH=7.0-8.0, and quantity of the immobilized microorganisms=0.75-1g/50 mL. The removal rates of TOC, TP, and TN under the above conditions were 80.2., 81.6%, and 86.8%, respectively. Conclusion The amount of heterotrophic bacteria in the two lakes was correlated with TOC, TP, and TN. These bacteria could be acclimatized and prepared for the immobilized microorganisms which could effectively remove nitrogen, phosphorus, and mixed organic pollutants in the water sample.

关键词： Heterotrophic bacteria Acclimation Immobilized microorganisms Nitrogen Phosphorus Mixed organic pollutants

专业心理求助态度在家庭教养方式与中学生非自杀性自伤间的中介作用

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现代预防医学 2024年第18期51卷 3398-3403页

作者：王群罗鹏张江萍陈思银徐孟群彭晓唯潘胜国李秀玲汪俊华贵州医科大学公共卫生与健康学院环境污染与疾病监控教育部重点实验室贵阳市云岩区卫生健康局贵阳市云岩区疾病预防控制中心

目的探究专业心理求助态度在家庭教养方式与中学生非自杀性自伤(NSSI)间的中介作用。方法采用多阶段分层整群抽样方法，于2.2.年10—12.选取贵州省贵阳市2.区的12.中学的4 393名中学生为研究对象，采用自编一般情况调查表、中文版... 详细信息

目的探究专业心理求助态度在家庭教养方式与中学生非自杀性自伤(NSSI)间的中介作用。方法采用多阶段分层整群抽样方法，于2.2.年10—12.选取贵州省贵阳市2.区的12.中学的4 393名中学生为研究对象，采用自编一般情况调查表、中文版简式父母教养方式问卷、专业心理支持求助态度简版量表、中国青少年NSSI评定问卷对中学生进行调查。使用SPSS 2..0对数据进行描述性分析、相关性分析，使用PROCESS 4.1程序进行中介效应分析，Bootstrap法进行验证。结果中学生NSSI检出率为34.9%。积极家庭教养方式与专业心理求助态度呈正相关、与NSSI呈负相关(r=0.308,-0.191,P1);消极家庭教养方式与专业心理求助态度呈负相关、与NSSI呈正相关(r=-0.2.3,0.310,P1);专业心理求助态度与NSSI呈负相关(r=-0.190,P1)。专业心理求助态度在积极家庭教养方式、消极家庭教养方式与NSSI间均发挥部分中介作用，中介效应占总效应分别为2..76%、8.87%。结论专业心理求助态度在家庭教养方式与中学生NSSI间发挥中介作用。父母应采取积极的教养方式、提高中学生专业心理求助的积极性，可预防和减少NSSI的发生。

关键词：中学生家庭教养方式专业心理求助态度 NSSI 中介作用

A356铝合金挤压铸造凝固过程及热处理对其性能的影响

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特种铸造及有色合金 2024年第2期44卷 189-197页

作者：邱谨曹杨婧罗晓梅周宏伟曹驰毕广利李元东兰州理工大学省部共建有色金属先进加工与再利用国家重点实验室兰州730050 兰州理工大学有色金属合金及加工教育部重点实验室兰州730050 兰州理工大学温州泵阀工程研究院温州325102

采用数值模拟和金相组织分析研究了A356铝合金挤压铸造成形顺序及凝固过程,发现半固态挤压铸造不仅充形平稳,凝固时间短,且孔隙率低。通过固溶、时效热处理可以改善金属型铸造、液态挤压铸造、半固态挤压铸造试样的组织及性能。结果表明... 详细信息

采用数值模拟和金相组织分析研究了A356铝合金挤压铸造成形顺序及凝固过程,发现半固态挤压铸造不仅充形平稳,凝固时间短,且孔隙率低。通过固溶、时效热处理可以改善金属型铸造、液态挤压铸造、半固态挤压铸造试样的组织及性能。结果表明,固溶处理后α-Al基体晶粒形貌与铸态相同,晶粒尺寸稍有长大,共晶Si球化尺寸集中在1.5μm左右。随着固溶温度升高,力学性能与热导率先增加后减小,而半固态挤压铸造后合金力学性能在560℃下固溶时并未降低。进一步时效处理后,过饱和的固溶体中析出弥散分布的Mg_(2.Si强化相,其不仅能够阻碍位错运动,提高A356铝合金的抗拉强度,而且减少了固溶原子与空位对电子的散射作用,提高导热性能。540℃×4 h固溶+180℃×9 h时效处理后,半固态挤压铸造试样性能最佳,其抗拉强度、伸长率、热导率分别为2.3.65 MPa、13.2.、191.82.W/(m·K)。

关键词：挤压铸造半固态数值模拟热处理热导率

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Simultaneous Electrochemical Determination of Ascorbic Acid, Dopamine and Uric Acid Based on Reduced Graphene Oxide-Ag/PANI Modified Glassy Carbon Electrode

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Chemical Research in chinese Universities 2017年第3期33卷 507-512页

作者： GUO Zhuo luo Xianke li Yahui li Dongdi ZHAO Qinai li Mengmeng MA chi ZHAO Yanting Department of Materials Science and Enginee~qng Shenyang University of Chemical Technology Shenyang 110142 R R. China Shenzhen Graduate School of Harbin Institute of Technology Shenzhen 518055 R R. China

An electrochemical method for simultaneous determination of ascorbic acid（AA）, dopamine（DA） and uric acid（UA） was developed based on RGO-Ag/PANI modified glassy carbon electrode（RGO-Ag/PANI/GCE, RGO= reduced graphene oxide, PANI=polyaniline）. Owing to the synergetic effect among RGO, Ag and PANI, the RGO-Ag/PANI based sensor not only improved the electrochemically catalytic oxidation ofAA, DA and UA, but also resolved the overlapping anodic peaks. Under the optimized conditions, the calibration curves were obtained in the concentration ranges of 50--1000, 5--2.0 and 2.--350 ttmol/L, and the detection limits（S/N=3） of 0.5, 0.2.and 0.2.~tmol/L were obtained for AA, DA, and UA, respectively, in neutral phosphate buffer solutions（PBS）. The RGO-Ag/PANI/GCE sensor was successfully applied to the determination of AA, DA, and UA in bovine serum samples and showed high selectivity, sensitivity, and reproducibility.

关键词： Dopamine Uric acid Ascorbic acid Electrochemical measurement

A spatial-explicit dynamic vegetation model that couples carbon,water,and nitrogen processes for arid and semiarid ecosystems

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Journal of Arid Land 2013年第1期5卷 102-117页

作者： chi ZHANG ChaoFan li Xi CHEN GePing {2. LongHui li XiaoYu li Yan YAN Hua SHAO State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 China University of the chinese Academy of Sciences Beijing 100049 China

Arid and semiarid ecosystems, or dryland, are important to global biogeochemical cycles. Dryland＇s community structure and vegetation dynamics as well as biogeochemical cycles are sensitive to changes in climate and atmospheric composition. Vegetation dynamic models has been applied in global change studies, but the com- plex interactions among the carbon （C）, water, and nitrogen （N） cycles have not been adequately addressed in the current models. In this study, a process-based vegetation dynamic model was developed to study the responses of dryland ecosystems to environmental changes, emphasizing on the interactions among the C, water, and N proc- esses. To address the interactions between the C and water processes, it not only considers the effects of annual precipitation on vegetation distribution and soil moisture on organic matter （SOM） decomposition, but also explicitly models root competition for water and the water compensation processes. To address the interactions between C and N processes, it models the soil inorganic mater processes, such as N mineralization/immobilization, denitrifica- tion/nitrification, and N leaching, as well as the root competition for soil N. The model was parameterized for major plant functional types and evaluated against field observations.

关键词： process-based model arid ecosystem C cycle vegetation dynamics

基于粪便代谢组学的不同蒸晒程度何首乌改善阿尔茨海默病认知障碍的实验研究

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中国中药杂志 2024年第8期49卷 2147-2157页

作者：周观琳李绍燕罗喜陈泓池鑫宇刘舒凌杨柯曾春晖广西中医药大学药学院南宁530000

通过粪便代谢组学方法研究何首乌改善阿尔茨海默病(Alzheimer′s disease,AD)认知障碍的作用机制,并比较不同蒸晒程度何首乌在改善AD模型小鼠认知功能上的作用差异,探讨其炮制原理。选取48只5月龄APP/PS1小鼠随机分为模型组、阳性组、... 详细信息

通过粪便代谢组学方法研究何首乌改善阿尔茨海默病(Alzheimer′s disease,AD)认知障碍的作用机制,并比较不同蒸晒程度何首乌在改善AD模型小鼠认知功能上的作用差异,探讨其炮制原理。选取48只5月龄APP/PS1小鼠随机分为模型组、阳性组、生品组、三蒸三晒组、六蒸六晒组、九蒸九晒组,另取7只同窝阴性对照小鼠作为空白组。灌胃给药150 d后,采用巴恩斯迷宫和避暗实验评估各组小鼠的学习记忆能力,并采集粪便样本进行广泛靶向代谢组学检测,结合主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)等多元统计方法对小鼠粪便中的代谢物进行分析。通过对比模型组和不同炮制品组的行为学结果显示,六蒸六晒组在巴恩斯迷宫定位航行测试中潜伏期显著缩短(P九蒸九晒组>生品组>三蒸三晒组,分别包含146、12.、95和81种潜在生物标志物。其中,与AD疾病相关的差异代谢物共有16种。基于炮制程度的进一步比较,发现六蒸六晒何首乌组在总代谢通路的调节数量最多,主要回调戊糖和葡萄糖醛酸相互转化和调节氨基酸合成代谢等途径。综上,何首乌经炮制后改善APP/PS1小鼠的学习记忆能力的作用机制可能与改善氨基酸代谢以及提高机体能量代谢水平有关,以六蒸六晒炮制程度最佳。

关键词：何首乌蒸晒炮制粪便代谢组学阿尔茨海默病氨基酸代谢能量代谢