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Differential Expression of Three Hypoxia-inducible Factor-α Subunits in Pulmonary Arteries of Rat with Hypoxia-induced Hypertension

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 665-672页

作者： Qi-Fang LI Ai-Guo DAI Department of respiratory Medicine,Human institute of Gerontology,Human Province Geriatric Hospital,Changsha 410001,china Department of Anesthesiology.Renji Hospital,Shanghai Second medical University,Shanghai,china Department of respiratory Medicine Hunan Institute of Gerontology Hunan Province Geriatric HospitalChangsha 410001 China

Hypoxia inducible transcription factor (HIF)-1α plays an important role in the development ofhypoxic pulmonary hypertension,but little is known about HIF-2α and HIF-3α with respect to transcriptionalregulation by *** examine the expression patterns of all HIF-α subunits (HIF-α,HIF-2α andHIF-3α) in pulmonary arteries of rats undergoing systemic hypoxia,five groups of healthy male Wistarrats were exposed to normoxia (N) and hypoxia for 3 (H),7 (H),14 (Hand 21 (*** arterial pressure (mPAP),vessel morphometry and right ventricular hypertrophy index *** were inflation fixed for immunohistochemistry and in situ hybridization,and homogenizedfor Western blot. mPAP increased significantly after 7 d of hypoxia [(18.4±0.4) vs. (14.4±0.4) mmHg,Hvs.N],reached its peak after 14 d of hypoxia,then remained *** artery remodeling and rightventricular hypertrophy developed significantly after 14 d of *** normoxia,HIF-1α and HIF-3αstaining were slightly positive regarding mRNA levels.A substantial alteration of HIF-1α and HIF-3α stainingoccurred in puhnonary arteries after 14 d and 7 d of hypoxia,respectively,but HIF-2α staining showed aninversed trend after 14 d of *** levels of all HIF-α subunits except HIF-3α showed a markedincrease corresponding to the duration of hypoxia,which was obtained by Western *** study foundthat HIF-1α. HIF-2α and HIF-3α may not only confer different target genes,but also play key pathogeneticroles in hypoxic-induced pulmonary hypertension.

关键词： hypoxia inducible factor-α hypoxia hypertension lung

Study on Substrate Specificity at Subsites for Severe Acute respiratory Syndrome Coronavirus 3CL Protease

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Acta Biochimica et Biophysica Sinica 2005年第12期37卷 807-813页

作者： Yu-Fei SHAN~1 Gen-Jun XU~(1,2)1 institute of Bichemistry and Cell Biology.Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 2 college of Life Science Zhejiang Sci-Tech University,Hangzhou 310018,china institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China.

Autocleavage assay and peptide-based cleavage assay were used to study the substratespecificity of 3CL protease from the severe acute respiratory syndrome *** was found that therecognition between the enzyme and its substrates involved many positions in the substrate,at least includingresidues from P4 to P2′.The deletion of either P4 or P2′residue in the substrate would decrease its cleavageefficiency *** contrast to the previous suggestion that only small residues in substrate could beaccommodated to the S1′subsite,we have found that bulky residues such as Tyr and Trp were also *** addition,based on both peptide-based assay and autocleavage assay,Ile at the P1′position could not behydrolyzed,but the mutant L27A could hydrolyze the Ile peptide *** suggested that there was astereo hindrance between the S1′subsite and the side chain of Ile in the *** 20 amino acids exceptPro could be the residue at the P2′position in the substrate,but the cleavage efficiencies were *** specificity information of the enzyme is helpful for potent anti-virus inhibitor design and usefulfor other coronavirus studies.

关键词： SARS coronavirus 3CL protease substrate specificity autocleavage binding site inhibitor design

Effects of CpG oligodeoxynucleotide on transcription factors GATA-3 and T-bet mRNA expression in asthmatic mice

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Acta Pharmacologica Sinica 2005年第9期26卷 1117-1122页

作者： Zu-qun WU~(1,2) Yi-ping XU~3 Hua XIANG~4 Hua-hao SHEN~11 Department of respiratory Medicine,the Second Affiliated Hospital,4 Department of Pathology,the First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,china 3 Department of respiratory Medicine,Renji Hospital,Shanghai Second medical University Shanghai 200001,china Department of respiratory Medicine the Second Affiliated Hospital Hangzhou China Department of respiratory Medicine Renji Hospital Shanghai Second Medical University Shanghai China Department of Pathology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China

Aim:To investigate effects ofCpG oligod'eoxynucleotide(CpG ODN)on the mRNAexpression of transcription factors GATA binding protein 3(GATA-3)and T-boxexpressed in T cells(T-bet)in asthmatic ***:An asthmatic mousemodel was established and treated with CpG *** inflammatory cells andeosinophils in bronchoalveolar lavage fluid(BALF)were counted and inflamma-tory cell infiltration in lung tissue was ***-y and interleukin-4concentrations in BALF and splenocyte culture supernatants were detected us-ing an enzyme-linked immunosorbent *** factor GATA-3 and T-bet mRNA expression in splenocytes and lung tissue were detected by reversetranscription-polymerase chain ***:Total inflammatory cells andeosinophils in BALF were reduced in the CpG ODN-treated group compared withthe asthma group,and inflammatory cell infiltration in lung tissue was also signifi-cantly *** ODN treatment increased the interferon-γ concentrationbut decreased the interleukin-4 concentration in both BALF and splenocyte cul-ture ***-3 mRNA expression was reduced in both lung tissueand splenocytes in the CpG ODN-treated group,while the mRNA ratio ofT-bet toGATA-3 in splenocytes was ***:CpG ODN treatment inhibitsairway inflammatory cell infiltration and regulates interferon-y/interleukin-4 syn-thesis in asthmatic mice,possibly through a mechanism of downregulation ofGATA-3 mRNA expression in both lung tissue and splenocytes.

关键词： asthma inflammation CpG oligodeoxy-nucleotide GATA-3 T-bet mice

CD39+ Regulatory T cells Suppress Generation and Differentiation of Th17 Cells in Malignant Pleural Effusion

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CD39+ Regulatory T cells Suppress Generation and Differentia...

中华医学会呼吸病学年会——2011（第十二次全国呼吸病学学术会议）

作者： Qiong Zhou 1 1 Department of respiratory diseases,Key Laboratory of Pulmonary diseases of Health Ministry,Union Hospital,Tongji medical college,Huazhong University of Science and Technology,Wuhan 430022,PR china 2 institute of respiratory diseases,First Affiliated Hospital,Guangxi medical University,Nanning 530021,PR china

Objective Both regulatory T cells(Tregs) and T helper IL-17-producing cells(Th17 cells) have been found to be involved in human malignancies,however,the possible implication of Tregs in regulating generation and differentiation of Th17 cells in malignant pleural effusion remains to be elucidated. Methods The numbers of both CD39 Tregs and Th17 cells in malignant pleural effusion and peripheral

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多项肿瘤标志物对肺癌的诊断价值

同位素 1996年第2期9卷 117-119页

作者：孙宝清陈顺存钟南山广州呼吸病研究所

采用放射免疫测定法对６０例肺癌患者、６０例良性肺部疾患患者进行β２－微球蛋白（β２－ＭＧ）、血清分泌型ＩｇＡ（ＳＩｇＡ）、铁蛋白（ＳＦ）和癌胚抗原（ＣＥＡ）含量的血清学联合检测。结果显示：肺癌组此四项血清肿瘤标志物均... 详细信息

采用放射免疫测定法对６０例肺癌患者、６０例良性肺部疾患患者进行β２－微球蛋白（β２－ＭＧ）、血清分泌型ＩｇＡ（ＳＩｇＡ）、铁蛋白（ＳＦ）和癌胚抗原（ＣＥＡ）含量的血清学联合检测。结果显示：肺癌组此四项血清肿瘤标志物均高于良性肺部疾患组及对照组，其阳性率分别为６６．７％、７０．０％、５３．５％和６０．０％，而６０例肺癌组中无一例出现四项检测均阴性者。因此，β２－ＭＧ、ＳＩｇＡ、ＳＦ和ＣＥＡ含量的测定均可作为诊断肺癌的辅助指标，且不同肿瘤标志物联合检测可明显提高肺癌检出率。

关键词：肺癌肿瘤标志物联合检测放射免疫测定

乙型肝炎病毒多表位抗原基因真核表达载体的构建及其在哺乳动物细胞中的表达

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现代免疫学 2004年第5期24卷 413-416页

作者：骆利敏李明夏虎黄建生吴英松第一军医大学热带病研究室广州510515 第一军医大学附属珠江医院呼吸科广州510280

将自行设计、合成的乙型肝炎病毒多表位抗原基因BPT ,克隆入真核表达载体pCDNA3 1,构建重组质粒pCDNA3 1/BPT。后者经脂质体法转染小鼠BALB/c 3T3细胞 ,G4 18加压筛选出阳性转染细胞 ,并用间接免疫荧光法鉴定其在小鼠BALB/c 3T3细胞的... 详细信息

将自行设计、合成的乙型肝炎病毒多表位抗原基因BPT ,克隆入真核表达载体pCDNA3 1,构建重组质粒pCDNA3 1/BPT。后者经脂质体法转染小鼠BALB/c 3T3细胞 ,G4 18加压筛选出阳性转染细胞 ,并用间接免疫荧光法鉴定其在小鼠BALB/c 3T3细胞的表达。该重组质粒经胫骨前肌注射小鼠 ,10 0 μg/次每只小鼠 ,间隔 2周加强一次 ,共 3次。免疫诱发了特异性抗体和淋巴细胞增殖反应 ,并用PCR法在小鼠的心脏、肝脏及肺组织中检测到pCDNA3 1/BPT 。

关键词：乙型肝炎 DNA疫苗转染免疫应答

A Study of the Impact of PE Optional Courses on Female college Students’ Fitness Health

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A Study of the Impact of PE Optional Courses on Female Colle...

Real-World Treatment Adherence to Smoking Cessation Medications and Association with Treatment Outcome—27 Centers,china,2017–2022

2012 International Conference on Education Technology and Management Engineering(ETME 2012)

作者： Dongsheng Wang South china Business college Guangdong University of Foreign Studies guangzhou 510545

In order to study the impact of PE optional courses on female college students’ fitness health,this paper respectively explores into the impact of such PE optional courses as dancesport,basketball,volleyball,badminton,Taekwondo by means of experimentation,measurement,and mathematical statistics,*** as can be seen from its results,the influence of these courses on female college students’ fitness health differs greatly from each other-basketball courses can enhance students’ quality of explosive force more than badminton;Taekwondo can improve their strength quality more than dancesport does;and compared with Taekwondo,dancesport,volleyball courses perform better in boosting students’ function of respiratory system;while as for other qualities and functions,as well as the body shape development,these courses have not shown a significant *** it is suggested that further study should be conducted on the causes and inherent laws of such significant differences in the impact of PE optional courses on students’ fitness health,so as to provide some reference to the theory and practice of PE optional *** the deepening of college PE teaching reforms,PE optional courses have already turned into a basic organizational form of PE and health curriculum in regular colleges and exhibited a unique charm,favored by a wide range of *** what is the impact on female college students’ fitness health of different optional courses? And how can curriculum management and practice efficiently regulate and control different items of teaching? It is always a crucial issue to correctly guide students to select courses independently on scientific *** this paper,through comparison and contrast between the results of a year’s experiment on the variational influence on female college students’ fitness health of such PE optional courses as dancesport,basketball,volleyball,badminton and Taekwondo,tends to provide some reference to the theory and practice of PE

关键词： PE optional course Female college student Fitness health Physical fitness

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china CDC weekly 2024年第22期6卷 522-529,I0001-I0003页

作者： Anqi Cheng Zhao Liu Xinmei Zhou Zheng Su Dan Xiao Chen Wang the china National Tobacco Cessation Cohort Study(CNTCCS)Study Group Department of Tobacco Control and Prevention of respiratory Disease Center of Respiratory MedicineChina-Japan Friendship Hospital/WHO Collaborating Center for Tobacco Cessation and Respiratory Diseases Prevention/National Center for Respiratory Medicine/National Clinical Research Center for Respiratory Diseases/Institute of Respiratory MedicineChinese Academy of Medical SciencesBeijingChina Department of Pulmonary and Critical Care Medicine Center of Respiratory MedicineChina-Japan Friendship Hospital/WHO Collaborating Center for Tobacco Cessation and Respiratory Diseases Prevention/China National Center for Respiratory Medicine/China National Clinical Research Center for Respiratory DiseasesBeijingChina Chinese Academy of medical Sciences and Peking Union medical college BeijingChina 不详

What is already known about this topic?Medication non-adherence significantly impedes smoking cessation *** effective smoking cessation medications are accessible in china,realworld adherence and its correlation with treatment outcomes remain largely *** is added by this report?From December 2017 to January 2022,1,120 participants were recruited from 27 centers in all seven geographical regions of *** revealed that only 38.2%of smokers adhered to the prescribed 12-week smoking cessation treatment and those with a higher education level were more likely to be *** addition,smokers with good adherence were more likely to successfully quit *** are the implications for public health practice?The data obtained could enhance our understanding of smoking cessation practices in *** adherence is a crucial strategy to enhance the effectiveness of smoking cessation treatments and management provided to smokers.

关键词： china smoke likely

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食管膈肌肌电图在呼吸机触发中的应用

中国实用内科杂志 2004年第11期24卷 659-660页

作者：郑则广陈荣昌王思华陈思蓓罗群李寅环黎毅敏郑劲平钟南山广州医学院第一附属医院广州呼吸病研究所 510120 暨南大学 510632

目的　选自 2 0 0 2 - 0 12 0 0 4 - 0 6以广州医学院第一附属医院的 4名健康医务工作者为研究对象 ,探讨食管膈肌肌电图 (EMG)应用于呼吸机触发的可行性。方法　实时记录 4名正常人膈肌肌电图和流速的变化 ,建立肌电图触发模型并比较... 详细信息

目的　选自 2 0 0 2 - 0 12 0 0 4 - 0 6以广州医学院第一附属医院的 4名健康医务工作者为研究对象 ,探讨食管膈肌肌电图 (EMG)应用于呼吸机触发的可行性。方法　实时记录 4名正常人膈肌肌电图和流速的变化 ,建立肌电图触发模型并比较其触发时机与吸气流量出现时机的差异性。结果　肌电图模拟触发信号的特异性和敏感性均达10 0 % ,触发时机比流量出现变化时机早 (2 0± 5 0 )ms。

关键词：食管膈肌肌电图呼吸机触发人机同步数学模型人工通气