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Deguelin inhibits expression of IκBα protein in Raji and U937 cells

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Acta Pharmacologica Sinica 2006年第4期27卷 485-490页

作者： Wei-hua CHEN Yan CHEN~2.Guo-hui CUI department of Hematology,Union Hospital.tongji {2. college,huazhong university of science and technology,wuhan 43002.,China department of Hematology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To determine whether deguelin can regulate the expression of nuclear factorkappa B(NF-κB)binding protein(IκBα)in U937 human leukemia cells and Rajihuman B lymphoma ***:The localization of IκBα protein was investi-gated by using an immunofluorescence *** expression of IκBα and NF-κB/p65 proteins in Raji and U937 cells were investigated by using Western *** was detected through annexin V/PI double-labeled ***:IκBα localized in the cytoplasm in untreated and deguelin-treated *** with tumor necrosis factor α(TNF-α)or deguelin plus TNF-α for 15 min,there was a substantial reduction in the amount of IκBα *** expressionof IκBα was downregulated by deguelin in Raji and U937 *** inducedapoptosis in U937 ***:Deguelin inhibited the expression of IκBαprotein in U937 and Raji *** anti-proliferative activity of deguelin is relatedto the signal pathway of NF-κB.

关键词： deguelin IkBα nuclear factor kappa B tumor necrosis factor-α apoptosis Raji cells U937 cells

Intercellular Adhension Molecule-1 in the Pathogenesis of Heroin-induced Acute Lung Injury in Rats

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Journal of huazhong university of science and technology(medical {2.s) 2004年第5期24卷 430-432页

作者：周琼白明邹世清 department of Respiratory Diseases Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China department of Withdrawal Liyuan Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430072 China

The expression of intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of heroin-induced acute lung injury (ALI) in rats was investigated. The model of ALI was established by intravenous injection of heroin into tail vein in rats. Thirty-six rats were randomly divided into heroin-treated groups (1 h, 2.h, 4 h, 6 h and 2. h) and normal control group. Changes in histopathologic morphology and biological markers of ALI were measured. The expression of ICAM-1 in lung tissue was detected by using immunohistochemistry and RT-PCR. The results showed that the W/D ratio and protein contents in BALF of the heroin-treated groups were significantly higher than that of the control group (P1). The histopathological changes in the lung tissue were more obvious in heroin-treated groups. The ICAM-1 protein and mRNA expression in the lung tissue of heroin-treated groups were significantly increased as compared with that of the control group (P1), and correlated with the ALI parameters in a time-dependent manner. Increasing of ICAM-1 expression was involved in the formation of heroin-induced lung injury. Furthermore, the level of expression was positively correlated with the severity of lung injury.

关键词： heroin acute lung injury intercellular adhesion molecule-1

Influences of dibutyryl cyclic adenosine monophosphate and forskolin on human sperm motility in vitro

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Asian Journal of Andrology 2003年第2期5卷 113-115页

作者： Ji-Hong LIU, Yang LI, Zheng-Guo CAO, Zhang-Qun YEdepartment of Urology, tongji Hospital,{2., huazhong university of science and technology,wuhan 430030, China department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Aim: To study the influences of dibutyryl cyclic adenosine monophosphate (dbcAMP) and forskolin on human sperm motility in vitro. Methods: Semen samples, aseptically obtained by masturbation and prepared by swim-up technique from 2. fertile men, were incubated with different concentrations of dbcAMP and forskolin at 37 癈. Measurements were carried out after 10 min, 2. min, 30 min and 60 min incubation. Motility parameters were estimated by using an automatic analyzing system. Results: Treatment with dbcAMP or forskolin resulted in a significant increase in sperm motility and progressive motility. The larger the concentrations of dbcAMP or forskolin, the greater the effect appeared. The straight linear velocity and curvilinear velocity were not affected by both agents. Conclusion: dbcAMP and forskolin increase the motility and progressive motility of human sperm in vitro.

关键词： dibutyryl cyclic AMP forskolin sperm motility

Bradykinin potentiates 5-HT3 receptor-mediated current in rat trigeminal ganglion neurons

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Acta Pharmacologica Sinica 2005年第4期26卷 428-434页

作者： Wang-ping HU~(1,2. Xue-mei LI~2.Ji-liang WU~3 Min ZHENG~3 Zhi-wang LI~4 2.department of Physiology,3 department of Pharmacology,Xianning {2.,Xianning 437100,China 4 department of Neurobiology,tongji medical {2. of huazhong university of science and technology,wuhan 430030,China department of Physiology Xianning College Xianning China department of Pharmacology Xianning College Xianning China department of Neurobiology Tongji Medical College of Huazhong University of Science and Technology Wuhan China

Aim:To explore the modulatory effect of bradykinin （BK） on 5-HT3receptor-mediated current in trigeminal ganglion （TG） neurons in ***:The whole-cell patch-clamp technique was used to record 5-HT-activated currents (I5-HT) inneurons freshly dissociated from rat *** were applied by rapid ***:The majority of the neurons examined responded to 5-HTapplied externally with an inward current （76.3%,74/97） that could be blocked bythe 5-HT3receptor antagonist,ICS-2.5,930 (1×10-6mol/L).In 66 of the 74 cellssensitive to 5-HT （89.2.）,pretreatment for 30 s with BK (1×10-6-1×10-10mol/L)could potentiate I5-HTwith the maximal modulatory effect occurring at10-7mol/L BK （71.6%+4.9%）.BK shifted the 5-HT concentration-response curveupwards with an increase of 68.9%±7.2. in the maximal current response,but withno significant change in the EC50value （19.1±3.2.μmol/L vs 2..9±3.5 μmol/L;t-test,P>0.05;n=8）.BK potentiated I5-HTin a holding potential-independent manner anddid not alter the reverse potential of *** BK-induced potentiation ofI5-HTwas almost completely blocked by Hoe 140 (5×10-7mol/L),a selective B2.Kreceptor antagonist,and was removed after intracellular dialysis of GF-1092.3X（2.μmol/L）,a selective protein kinase C （PKC） inhibitor,with the re-patch ***:Pre-application of BK exerts an enhancing effect on I5-HTvia a PKC-dependent pathway in rat TG neurons,which may explain the peripheral'mecha-nism of pain and hyperalgesia caused by,for example,tissue damage andinflammation.

关键词： bradykinin 5-HT3 serotonin receptor regulation patch-clamp techniques trigeminal ganglion

Risk Factors of Learning Disabilities in Chinese Children in wuhan

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Biomedical and Environmental sciences 2003年第4期16卷 392-397页

作者： BIN YAO AND HAN-RONG WUdepartment of Children and Adolescent Health, Maternity and Child Health Care, tongji medical college,{2., wuhan 430030, Hubei, China department of Children and Adolescent Health Maternity and Child Health Care Tongji Medical College Huazhong University of Science and Technology 湖北 Wuhan 430030

Objective To investigate prevalence rate of learning disabilities (LD) in Chinese children, and to explore related risk factors, and to provide theoretical basis for preventing such disabilities. Methods One thousand and one hundred fifty one children were randomly selected in primary schools. According to criteria set by ICD-10, 118 children diagnosed as LD were classified into the study group. Four hundred and ninety one children were classified into the normal control group. Five hundred and forty two children were classified into the excellent control group. The study instruments included PRS (The pupil rating scale revised screening for learning disabilities), Conners' children behavior check-list taken by parents and YG-WR character check-list. Results The prevalence rate of LD in Chinese children was 10.3%. Significant differences were observed between LD and normally learning children, and between the LD group and the excellent group, in terms of scores of Conners' behavior check-list (P<0.05). The study further showed that individual differences in character between the LD group and the control groups still existed even after controlling individual differences in age, IQ, and gender. Some possible causal explanations contributing to LD were improper teaching by parents, low educational level of the parents, and children's characteristics and social relationships. Conclusion These data underscore the fact that LD is a serious national public health problem in China. LD is resulted from a number of factors. Good studying and living environments should be created for LD children.

关键词： Learning disabilities (LD) Behavior problems Character Children Risk factors

Effects of Carvedilol on Cardiomyocyte Apoptosis and Gene Expression In Vivo after Ischemia-Reperfusion in Rats

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Journal of huazhong university of science and technology(medical {2.s) 2003年第2期23卷 127-130页

作者：曾和松刘晓春赵华月 department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 department of Neurobiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

The effects of carvedilol on cardiomyocyte apoptosis and expression of bcl-2. bax genes following ischemia (0. 5 h) and reperfusion (48 h) in vivo and the possible biological mechanism of carvedilol inhibiting cardiomyocyte apoptosis were studied. The left anterior descending artery in Wistar rats were ligated to establish ischemia-reperfusion (I/R) models. The model animals were divided into two groups: I/R group, the model rats not subject to other treatments except ischemia-reperfusion (n= 8); carvedilol-treated group (n= 8), I/R model rats treated with carvedilol. Eight rats in the sham-operated group were subjected to only experimental open operation. The number of apoptotic cardiomyocyte was determined by TUNEL staining. Immunohistochemistry and in situ hybridization histochemistry (ISHH) were used to detect the expression of bcl-2.and bax genes. Image processing system was used to quantitatively dispose the positive metric substances of both immuno-histochemistry and ISHH through the average optical density (OD) value. The results showed that the number of the apoptotic cells were 36. 18±9 (I/R group) . 0-1 (sham-operated group) and 9. 5± 3 (carvedilol-treated group) in each visual field respectively with the difference being very significant among the groups (P1). The OD values of bcl-2.protein in sham-operated group, I/R group and carvedilol-treated group were 0. 14±0. 01, 0. 08±0. 02.and 0. 15±0. 03, respectively. The OD values of bcl-2.mRNA in sham-operated group, I/R group and carvedilol-treated group were 0. 08± 0. 01, 0. 06±0. 01 and 0. 09±0. 01, respectively. There was no significant difference between carvedilol-treated group and I/R group (P>0. 05). The OD values of bax protein in I/R group, sham-operated and carvedilol-treated-treated group were 0. 13±0. 02. 0. 07±0. 01, 0. 06±0. 01, respectively. There was very significant difference between carvedilol-treated group and I/R group (P 1). Bcl-2.bax ratio was 1. 07±0. 14 (I/R group), 1. 2.

关键词： carvedilol ischemia-reperfusion apoptosis gene expression

Expression and Location of Intracellular Tissue Factor in Atherosclerosis Stable Plaque of ApoE^(-/-) Mice

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Journal of huazhong university of science and technology(medical {2.s) 2009年第4期29卷 457-461页

作者：李军陈涛王定淼宋毅峰洪梅 department of Cardiothoracic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Insititute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

In the ApoE^-/- mouse model of atherosclerosis （AS） stable plaque, the expression and location of intracellular tissue factor （TF） in the cellular components of AS stable plaque were investigated in order to explore the cellular mechanism of AS thrombosis. Pathological changes of the stable plaque were observed under a microscope. The expression of TF protein was examined in aortic stable plaque of mice by using immunohistochemistry. Color image planimetric system was used to analyze the histological components of the stable plaque and the TF distribution. Under the confocal microscope, the intracellular TF location in the stable plaque of mice was observed. The results showed the cellular area was the major part of stable plaque （67.36%±6.52., P〈0.01）. The percentage of total area occupied by cellular area was significantly larger than atheromatous gruel and acellular area （P〈0.01）. Macrophages and smooth muscle cells （SMC） were major cells in the cellular area. The percentage of total area occupied by SMC was significantly larger than by macrophages （P〈0.01）. Multiple linear regression analysis showed there was a positive correlation between TF area and SMC area （r=0.616, P=-0.008）, and no correlation was found between TF area and macrophage area （r=0.437, P=0.08）. Pictures of color image planimetric analysis of TF and SMC were merged to highlight areas with co-localization （yellow）, it was concluded that the process could be a cell-mediated TF expression in the stable plaque. SMC may be the major source of TF in AS without plaque rupture.

关键词： atherosclerosis stable plaque tissue factor Apo E knockout mice

All-trans Retinoic Acid Diminishes Collagen Production in a Hepatic Stellate Cell Line via Suppression of Active Protein-1 and c-Jun N-terminal Kinase Signal

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Journal of huazhong university of science and technology(medical {2.s) 2010年第6期30卷 726-733页

作者：叶媛但自力 department of Gastroenterology Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

Following acute and chronic liver injury,hepatic stellate cells (HSCs) become activated to undergo a phenotypic transformation into myofibroblast-like cells and lose their retinol content,but the mechanisms of retinoid loss and its potential roles in HSCs activation and liver fibrosis are not *** influence of retinoids on HSCs and hepatic fibrosis remains *** purpose of this study was to evaluate the effects of all-trans retinoid acid (ATRA) on cell proliferation,mRNA expression of collagen genes [procollagen α1 (Ⅰ),procollagen α1 (Ⅲ)],profibrogenic genes (TGF-β 1,CTGF,MMP-2.TIMP-1,TIMP-2.PAI-1),fibrolytic genes (MMP-3,MMP-13) and the upstream element (JNK and AP-1) in the rat hepatic stellate cell line (CFSC-2.).Cell proliferation was evaluated by measuring BrdU *** mRNA expression levels of collagen genes [procollagen α1 (Ⅰ),procollagen α1 (Ⅲ)],profibrogenic genes (TGF-β 1,CTGF,MMP-2.TIMP-1,TIMP-2.PAI-1),and fibrolytic genes (MMP-3,MMP-13) were quantitatively detected by using real-time *** mRNA expression of JNK and AP-1 was quantified by *** results showed that ATRA inhibited HSCs proliferation and diminished the mRNA expression of collagen genes [procollagen α1 (Ⅰ),procollagen α1 (Ⅲ)] and profibrogenic genes (TGF-β 1,CTGF,MMP-2.TIMP-1,TIMP-2.PAI-1),and significantly stimulated the mRNA expression of MMP-3 and MMP-13 in HSCs by suppressing the mRNA expression of JNK and *** findings suggested that ATRA could inhibit proliferation and collagen production of HSCs via the suppression of active protein-1 and c-Jun N-terminal kinase signal,then decrease the mRNAs expression of profibrogenic genes (TGF-β 1,CTGF,MMP-2.TIMP-1,TIMP-2.PAI-1),and significantly induce the mRNA expression of MMP-3 and MMP-13.

关键词： all trans-retinoic acid liver stellate cells collagen transforming growth factor β 1 active protein-1 c-Jun N-terminal kinase.

Reversal of P-glycoprotein-mediated Multidrug Resistance in SGC7901/VCR Cells by PPARγ Activation by Troglitazone

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Journal of huazhong university of science and technology(medical {2.s) 2010年第3期30卷 326-331页

作者：陈庆周洁蒋春舫陈娟 department of Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and Technology department of Biochemistry and Molecular Biology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and Technology

Over-expression of P-glycoprotein(P-gp),an ATP-dependent drug efflux pump,represents one of the major mechanisms that contribute to multidrug resistance(MDR) in cancer *** study examined the effects of troglitazone,a ligand of peroxisome proliferator-activated receptor gamma(PPARγ),on P-gp-mediated MDR in SGC7901/VCR cells(a vincristine-resistant human gastric cancer cell line).The expression of P-gp was detected by RT-PCR and Western blotting,*** SGC7901/VCR cells were treated with 0.1 mg/L vincristine(VCR) alone or in combination with 1,5,10 μmol/L troglitazone for 2. ***γ was measured by electrophoretic mobility shift assay(EMSA).The intracellular concentration of Rhodamine12.(Rh12.,a fluorescent P-gp substrate) was assayed to evaluate the activity of *** cell cycle and apoptosis were measured by flow *** results showed that the P-gp was increasingly expressed in SGC7901,BGC82. and SGC7901/VCR cells in turn,suggesting that MDR in the SGC7901/VCR cells was mediated by the increased expression of *** the SGC7901/VCR cells,the expression level of total PPARγ was increased,however,the protein level and activity of PPARγ in the nuclei of cells decreased *** elevated the PPARγ activity in SGC7901/VCR cells in a dose-dependent *** decreased the P-gp expression and markedly enhanced the accumulation of Rh12. in SGC7901/VCR cells in a dose-dependent *** also found that troglitazone significantly increased the percentage of SGC7901/VCR cells in the G2.M phase and decreased the cell percentage in G1 and S phase in a dose-dependent *** significantly increased the apoptotic rate of SGC7901/VCR cells treated by VCR or ADR in a dose-dependent *** was concluded that P-gp-overexpressed SGC7901/VCR cells have minor endogenous PPARγ *** of the PPARγ activity by troglitazone can reverse P-gp-mediated MDR via down-regulating the expression and activity of P-gp i

关键词： multidrug resistance peroxisome proliferator-activated receptor gamma P-glycoprotein troglitazone SGC7901/VCR cells