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IκB kinase Mediating the Downregulation of p53 and p21 by Lipopolysaccharide in Human Papillomavirus 16^＋ Cervical Cancer Cells

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Chinese Medical Journal 2016年第22期129卷 2703-2707页

作者： Zhi-hui tan Yu zhang Yan Tian wei tan Ying-hua Li Department of Gynecology Xiangya Hospital Central South University Changsha Hunan 410078 China

Background：Cervical cancer is the second most common cancer of woman in the world,and human papillomavirus （HPV） infection plays an important role in the development of most of the cases.IκB kinase β （IKKβ） is a kinase-mediating nuclear factor kappa B （NF-κB） activation by phosphorylating the inhibitor ofNF-κB （IκB） and is related by some diseases caused by virus ***,there is little known about the correlation between IKKβ and HPV infection in cervical *** study aimed to investigate the expression of IKKβ protein in cervical cancer tissues and effects of inflammation on HPV positive or negative cervical cancer cells through detecting the expression of IKKβ,IKBα,p53,and p21 proteins after treated with lipopolysaccharide （LPS） to mimic bacterial *** also examined the effects of LPS on cervical cancer cells after blocking IKKβ with pharmacological ***：Thirty-six matched specimens of cervical cancer and adjacent normal tissues were collected and analyzed in the *** expression of IKKβ in the tissue specimens was determined by immunohistochemical *** addition,Western blot was used to detect the expression level changes ofIKKβ,IκBα,p53,and p21 after LPS stimulated in the HPV16^＋（SiHa） and HPV16-（C33A） cervical cancer cell ***,the effects of IKKβ inhibitor SC-514 on LPS-induced expression change of these proteins were ***：The expression of IKKβ was higher in cervical cancer than adjacent normal tissues,and there was no significant difference between tumor differentiation,size,and invasive depth with IKKβ *** LPS,which increased the expression level of IKKβ protein but decreased in the IκBα,p53 and p21 proteins,was illustrated in HPV16^＋（SiHa） but not in HPV16^-（C33A） ***,IKKβ inhibitor SC-514 totally reversed the upregulation of IKKβ and downregulation of p53 and p21 by LPS in SiHa ***：IKKβ may mediate the downregulation of p53 and p21 by LPS in HP

关键词： Cervical Cancer IKKβ Lipopolysaccharide p21 p53

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高温放料阀流固耦合传热过程分析

阀门 2024年第4期 460-465页

作者：张道军陈昌华李威谭玄邓鑫雍华健四川苏克流体控制设备股份有限公司四川成都610200

本文以3"高温放料阀为研究对象,采用数值模拟方法,建立高温放料阀几何模型;分析计算高温放料阀模型在不同工况下,流固耦合传热过程中的换热情况;对放料阀的关键部位进行温度场分析,结果表明,当冷却水入口流速为2 m/s,温度为280 K时,阀... 详细信息

本文以3"高温放料阀为研究对象,采用数值模拟方法,建立高温放料阀几何模型;分析计算高温放料阀模型在不同工况下,流固耦合传热过程中的换热情况;对放料阀的关键部位进行温度场分析,结果表明,当冷却水入口流速为2 m/s,温度为280 K时,阀杆底部温度达到最低的310 K,满足设计要求。通过以上研究,本文使用的数值计算方法可以为高温放料阀流固换热分析提供参考,也为阀体和阀杆之间的温度分析提供数据支持。

关键词：高温放料阀数值模拟流固耦合温度场

Prediction model of surface integrity characteristics in ball end milling TC17 titanium alloy

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Advances in Manufacturing 2023年第3期11卷 541-565页

作者： Xue-hong Shen Chang-Feng Yao Liang tan Ding-hua zhang Key Laboratory of High Performance Manufacturing for Aero Engine Ministry of Industry and Information TechnologySchool of Mechanical EngineeringNorthwestern Polytechnical UniversityXi'an 710072People's Republic of China Engineering Research Center of Advanced Manufacturing Technology for Aero Engine Ministry of EducationSchool of Mechanical EngineeringNorthwestern Polytechnical UniversityXi'an 710072People's Republic of China

Surface integrity is important to improve the fatigue property of *** selection of the cutting parameters is extremely important in ensuring high surface *** this paper,ball end milling of TC17 alloy has been carried out utilizing response surface *** effects of cutting speed,feed per tooth,cutting depth,and cutting width on the surface integrity characteristics,including surface roughness(R_(a)),surface topography,residual stress,and microstructure were ***,predictive metamodels for surface roughness,residual stress,and microhardness as a function of milling parameters were *** to the experimental results obtained,the surface roughness increases with the increase of milling parameters,the(R_(a))values vary from 0.4μm to 1.2μm along the feed direction,which are much lower compared to that along the pick feed *** surface compressive residual stress increases with the increase of feed per tooth,cutting depth,and cutting width,while that decreases at high cutting *** depth of the compressive residual stress layer is mostly in the range of 25-40μ*** milled surface microhardness represents 6.4% compared with the initial state;the work-hardened layer depth is approximately 20μ***,plastic deformation and strain streamlines are observed within 3μm depth beneath the *** empirical model of surface integrity characteristics is developed using the results of ten experiments and validated by two extra *** prediction errors of the three surface integrity characteristics are within 27%;the empirical model of microhardness has the lowest prediction errors.

关键词： Prediction model Surface roughness(R_(a)) Residual stress Microhardness Microstructure

杨梅素通过激活Nrf2/HO-1通路抑制氧化应激改善肾脏纤维化

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药学学报 2024年第2期59卷 359-367页

作者：李冬雪黄周王瀚宇张之昊谭宁华邓雪阳中国药科大学中药学院江苏南京211198

本文探讨杨梅素(myricetin,MYR)对单侧输尿管结扎(unilateral uretera obstruction,UUO)和胆管结扎(common bile duct ligation,CBDL)诱导的小鼠肾脏纤维化的影响及其作用机制。动物实验已获得中国药科大学伦理委员会批准,项目伦理号为2... 详细信息

本文探讨杨梅素(myricetin,MYR)对单侧输尿管结扎(unilateral uretera obstruction,UUO)和胆管结扎(common bile duct ligation,CBDL)诱导的小鼠肾脏纤维化的影响及其作用机制。动物实验已获得中国药科大学伦理委员会批准,项目伦理号为2022-10-020。选取35只ICR小鼠,分为control组、UUO组、UUO+MYR组、CBDL组、CBDL+MYR组。H&E和Masson染色检测肾脏组织病理变化,蛋白质免疫印迹法(Western blot,WB)检测肾组织中纤维化相关蛋白的表达;总超氧化物歧化酶(superoxide dismutase,SOD)活性检测试剂盒(WST-8法)检测CBDL小鼠肾脏组织中的总SOD变化。体外实验采用HK-2细胞,细胞转化生长因子-β1(transforming growth factor beta 1,TGF-β1)(10 ng·mL^(-1))造纤维化模型,高糖(30 mmol·L-1)造氧化应激模型,随后使用不同浓度的MYR处理,WB检测纤维化及氧化应激相关蛋白的表达;使用不同浓度的MYR处理NIH/3T3细胞,5-溴-2′-脱氧尿苷(5-bromo-2′-deoxyuridine,Brdu)标记法检测其对细胞增殖的影响。结果显示,UUO组和CBDL组的肾脏病变严重,胶原沉积明显,I型胶原蛋白(collagen-I,COL-I)、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、纤连蛋白(fibronectin,FN)、波动蛋白(vimentin)、纤溶酶原激活物抑制物1(plasminogen activator inhibitor-1,PAI-1)蛋白表达上调,CBDL组小鼠肾脏SOD酶活力明显下降,MYR给药治疗后逆转了上述变化。MYR给药抑制NIH/3T3细胞的增殖活力而对HK-2细胞没有影响,降低了TGF-β1诱导HK-2细胞后PAI-1、FN、vimentin的上调。MYR给药也能上调高糖诱导HK-2细胞后核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)、血红素加氧酶1(heme oxygenase-1,HO-1)的下调。综上所述,MYR在体内外都可发挥改善肾脏纤维化的作用,可能是通过抑制成纤维细胞的增殖,同时激活Nrf2/HO-1信号通路抑制氧化应激发挥作用。

关键词：杨梅素单侧输尿管结扎胆管结扎肾纤维化氧化应激

盐度对全氟和多氟烷基物质(PFAS)在海水-沉积物界面吸附行为的影响

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中国环境科学 2024年第1期44卷 462-473页

作者：姜舒祺毕宇杰张涛冯志华耿倩倩谭志军郭萌萌江苏海洋大学海洋科学与水产学院江苏连云港222005 中国水产科学研究院黄海水产研究所山东青岛266071 海水养殖生物育种与可持续产出全国重点实验室山东青岛266071

模拟海水-沉积物环境,分析了不同盐度影响下5种全氟和多氟烷基物质(PFAS)含量变化,并解析了PFAS的吸附动力学和热力学等吸附行为.在设置的不同盐度实验组中,全氟辛烷磺酸(PFOS)在沉积物中的吸附能力强于相同碳氟链长度的全氟辛酸(PFOA)... 详细信息

模拟海水-沉积物环境,分析了不同盐度影响下5种全氟和多氟烷基物质(PFAS)含量变化,并解析了PFAS的吸附动力学和热力学等吸附行为.在设置的不同盐度实验组中,全氟辛烷磺酸(PFOS)在沉积物中的吸附能力强于相同碳氟链长度的全氟辛酸(PFOA),其中PFOS在沉积物中的吸附量大于其替代物9-氯十六氟-3-氧杂壬烷-1-磺酸钾(9Cl-PF3ONS);PFOA在沉积物中的吸附量大于其替代物六氟环氧丙烷二聚酸(HFPO-DA),而小于另一替代物六氟环氧丙烷三聚酸(HFPO-TA).PFAS的沉积物分配系数(Kd)与盐度呈正相关,随着盐度的升高,PFAS在沉积物中的吸附量随之增加.PFAS的吸附过程涉及了疏水和静电相互作用.

关键词：全氟和多氟烷基物质(PFAS) 海水沉积物盐度吸附新污染物

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木聚糖酶在青贮及反刍动物日粮中的应用

草地学报 2024年第1期32卷 331-339页

作者：刘伟于凤民马千鹏特古斯巴雅尔张晓华张兴军谭艺沅贾玉山内蒙古农业大学草原与资源环境学院/农业农村部饲草栽培、加工与高效利用重点实验室/草地资源教育部重点实验室内蒙古呼和浩特010019 巴林左旗农牧局内蒙古赤峰024000 巴林左旗林业和草原局内蒙古赤峰024000 巴林左旗工信和科技局内蒙古赤峰024000

青贮饲料与反刍动物日粮中木质纤维素占比高,直接进行利用会造成能量流失。酶制剂在青贮或日粮中应用广泛,通过酶制剂预处理可提高饲料的利用效率。木聚糖酶与其它酶制剂混合添加可有效降解青贮中的纤维含量,增加可代谢糖含量,有利于微... 详细信息

青贮饲料与反刍动物日粮中木质纤维素占比高,直接进行利用会造成能量流失。酶制剂在青贮或日粮中应用广泛,通过酶制剂预处理可提高饲料的利用效率。木聚糖酶与其它酶制剂混合添加可有效降解青贮中的纤维含量,增加可代谢糖含量,有利于微生物生长,促进青贮发酵,进而提高青贮饲料的利用效率。在反刍动物日粮中,木聚糖酶可以辅助瘤胃微生物分解饲料中的纤维成分,改善瘤胃发酵环境,使反刍动物获得更多的能量来供其生长和代谢,进而提升反刍动物的生产性能。本文介绍了木聚糖与木聚糖酶,通过综述木聚糖酶在青贮及反刍动物日粮中应用,阐释了木聚糖酶在青贮与反刍动物日粮中的添加量、利用效果,为后续木聚糖酶的利用提供理论参照。

关键词：木聚糖木聚糖酶青贮反刍动物日粮

Expression and Characterization of Human Heart Type Fatty Acid Binding Protein in Pichia Pastoris

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Chemical Research in Chinese Universities 2006年第2期22卷 157-161页

作者： HOU wei tan Yan XU Shu-fen YANG Xiao-hong zhang Shu-hua LIU Ling-li CHE Yuan-yuan LIU Li-hua Pharmacy College of Jilin University The First Hospital of Jilin University Changchun 130021 P. R. China Changchun University of Science and Technology Changchun 130021 P. R. China

H-FABP is regarded as a tissue-specific protein existing only in myocardial cells. It is released from the cardiac tissue and gets into the plasma when a heart attack occurs; the myocardial infarction is a good case in point. As a resuit, the detection of H-FABP will be an early and important biomarker for the disease concerned. The objective of the study is to prepare the recombinant H-FABP by aeukaryotic expression system, pichia, to produce the protein mimicking natural H-FABP, as an immunogen for the production of the specific antibody. A gene fragment encoding H-FABP was cloned in the expressing vector pPICZα, after sequencing. The recombinant plasmid was transformed into the competent cells of the X-33 strain by means of electroporation. The expression of the target peptide induced by methanol was screened by means of Western hlotting, with the available MAb（Clone 6B6）. Highly expressive engineer strains were obtained. The production of recombinant H-FABP under induction was about 0.7 g/L, with an Mr of 14.5 kDa and recognized by a commercially available MAb （Clone 6B6）. The recombinant vector was successfully constructed. Following this, H-FABP was expressed in X-33, and it would become the source of the preparation of specific antibodies, to develop diagnostic kits.

关键词： Human heart type fatty acid binding protein Expression and purification Pichia

Interaction between local corrosion and flow field of natural gas long-distance pipeline by artificial rectangular defect pit

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Journal of Iron and Steel Research International 2022年第6期29卷 1026-1038页

作者： Zhuo-wei tan Zhen-bo Wang Shu-yu Bai Da-lei zhang Sheng-zhu zhang Frank Cheng Shao-hua Xing You-hai Jin China University of Petroleum(East China) Qingdao 266580ShandongChina Xiangtan University Xiangtan 411105HunanChina School of Materials Science and Engineering China University of Petroleum(East China)Qingdao 266580ShandongChina China Academy of Safety Science and Technology Beijing 100012China University of Calgary CalgaryAB T2N 1N4Canada State Key for Marine Corrosion and Protection Luoyang Ship Material Research InstituteQingdao 266237ShandongChina

The corrosive environment in long-distance natural gas pipeline was simulated by the online high shear stress flow test *** interaction between flow fields and local corrosion in different local corrosion stages was studied by machining different depths rectangular defect pit(RDP)on X80 pipe steel *** electrochemical signals of each specimen under high shear stress flow were measured online using an integrated three-electrode and electrochemical *** spectroscopy confirmed that the corrosion scale of X80 pipeline steel in CO_(2)-saturated National Association of Corrosion Engineers solution was composed of FeCO_(3).The scanning electron microscope images displayed variations in microstructure of the corrosion scale at different RDP depths and different *** flow field fluctuations induced by RDP were analyzed by computational fluid dynamics simulations and the development of local corrosion pits was discussed in terms of integrity of corrosion scale,convective mass transfer,and diffusion mass transfer.

关键词： CO_(2) corrosion Flow condition Rectangular defect pit Local corrosion Corrosion electrochemistry

Minocycline protects against myocardial ischemia/reperfusion injury in rats by upregulating MCPIP1 to inhibit NF-κB activation

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Acta Pharmacologica Sinica 2019年第8期40卷 1019-1028页

作者： Quan Yi Fang-hui tan Jia-an tan Xiu-hui Chen Qing Xiao Ying-hua Liu Gui-ping zhang Jian-dong Luo Department of Pharmacology School of Pharmaceutical SciencesGuangzhou Medical University Guangzhou Institute of Cardiovascular Disease Guangzhou Key Laboratory of Cardiovascular Disease Second Affiliated Hospital Guangzhou Medical University

Minocycline is a tetracycline antibiotic and has been shown to play a protective role in cerebral and myocardial ischemia/reperfusion(I/R).However,the underlying mechanism remains ***,we investigated whether monocyte chemotactic protein-induced protein-1(MCPIP1),a negative regulator of inflammation,was involved in the minocycline-induced cardioprotection in myocardial I/R in vivo and in vitro *** ischemia was induced in rats by left anterior descending coronary artery occlusion for 1 h and followed by 48 h *** was administered prior to ischemia(45 mg/kg,ip,BID,for 1 d)and over the course of reperfusion(22.5 mg/kg,ip,BID,for 2 d).Cardiac function and infarct sizes were *** of minocycline significantly decreased the infarct size,alleviated myocardial cell damage,elevated left ventricle ejection fraction,and left ventricle fractional shortening following I/R injury along with significantly decreased pro-inflammatory cytokine IL-1β and monocyte chemoattractant protein-1(MCP-1)levels in heart tissue.H9c2 cardiomyocytes were subjected to oxygen glucose deprivation(OGD)followed by reoxygenation(OGD/R).Pretreatment with minocycline(1-50μmol/L)dosedependently increased the cell viability and inhibited OGD/R-induced expression of MCP-1 and ***,minocycline dose-dependently inhibited nuclear translocation of NF-κB p65 in H9c2 cells subjected to OGD/*** both the in vivo and in vitro models,minocycline significantly increased MCPIP1 protein expression;knockdown of MCPIP1 with siRNA in H9c2 cells abolished all the protective effects of minocycline against OGD/R-induced *** results demonstrate that minocycline alleviates myocardial/R injury via upregulating MCPIP1,then subsequently inhibiting NF-κB activation and pro-inflammatory cytokine secretion.

关键词： myocardial ischemia and reperfusion H9c2 cardiomyocytes oxygen glucose deprivation minocycline cytokine NF-κB monocyte chemotactic protein-induced protein-1