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甘肃陇南锑矿区污染及微生物群落结构特征

环境科学 2024年第7期45卷 4266-4278页

作者：赵清英张泽民谭昭李文杰潘丽波关潇李金花中国环境科学研究院生态研究所北京100012 兰州大学生态学院兰州730000 中国环境科学研究院国家环境保护区域生态过程与功能评估重点实验室北京100012

锑(Sb)是有色金属矿山开采和加工中对环境造成严重威胁的重点污染物,与砷(As)的硫化物和氧化物共存.微生物对土壤中重金属的迁移转化及修复具有重要意义.目前,锑矿不同功能分区(采矿区和冶炼区)中重金属有效态对土壤微生物群落结构的生... 详细信息

锑(Sb)是有色金属矿山开采和加工中对环境造成严重威胁的重点污染物,与砷(As)的硫化物和氧化物共存.微生物对土壤中重金属的迁移转化及修复具有重要意义.目前,锑矿不同功能分区(采矿区和冶炼区)中重金属有效态对土壤微生物群落结构的生态效应仍知之甚少.为掌握不同功能分区中重金属污染特征及重金属有效态与土壤微生物群落之间的相互作用关系,采用Wenzel连续提取法和16S r DNA高通量测序技术测定了Sb和As的化学形态及微生物信息.结果表明,Wenzel连续提取后Sb和As的化学形态分布占比大小为:无定型铁铝氧化物结合态(F3)>晶质铁铝氧化物结合态(F4)>残渣态(F5)>专性吸附态(F2)>非专性吸附态(F1).潜在生态风险指数(RI)和地累积指数(Igeo)显示,Sb污染程度大小为:冶炼区>采矿区>对照区,冶炼区为严重污染,采矿区为中度至重度污染;As污染程度大小为:采矿区>冶炼区>对照区,采矿区和冶炼区均为中度至重度污染.16S r DNA高通量测序显示:Proteobacteria是采矿区与冶炼区中相对丰度最高的门,Kaistobacter、Pseudomonas、Sphingomonas和Lysobacter是采矿区与冶炼区中相对丰度最高的属;Geobacter和Luteolibacter在采矿区有较高的LDA得分,Thiobacillus在冶炼区具有较高的LDA得分.Spearman相关性、方差分解(VPA)和随机森林预测(RF)表明,Sb、As、有效态锑[Sb(Bio)]和有效态砷[As(Bio)]是影响锑矿不同功能分区中微生物群落结构的主要因子;冗余分析(RDA)显示,对属水平微生物群落结构的影响大小为:As(Bio)>Sb(Bio)>Sb>As,Sb及Sb(Bio)和Nitrospira呈现显著负相关关系,和Thiobacillus呈现显著正相关关系(P<0.05).通过深入研究重金属污染特征及重金属有效态对微生物群落结构的生态效应,可为锑矿区生态修复和生态环境管理提供参考.

关键词：锑(Sb)矿区 Wenzel连续提取法生态风险评估微生物群落分析 16S rDNA高通量测序

Real-time Microwave Exposure Induces Calcium Efflux in Primary Hippocampal Neurons and Primary Cardiomyocytes

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Biomedical and Environmental Sciences 2018年第8期31卷 561-571页

作者： WANG hui zhang Jing HU Shao hua tan Sheng Zhi zhang Bo ZHOU Hong Mei PENG Rui Yun Department of Experimental Pathology Institute of Radiation Medicine Academy of Military Medical Sciences Department of Clinical Laboratory Shanghai Children's Hospital Shanghai Jiao Tong University

Objective To detect the effects of microwave on calcium levels in primary hippocampal neurons and primary cardiomyocytes by the real-time microwave exposure combined with laser scanning confocal microscopy. Methods The primary hippocampal neurons and primary cardiomyocytes were cultured and labeled with probes, including Fluo-4 AM, Mag-Fluo-AM, and Rhod-2, to reflect the levels of whole calcium [Ca], endoplasmic reticulum calcium [Ca]ER, and mitochondrial calcium [Ca]MIT, respectively. Then, the cells were exposed to a pulsed microwave of 2.856 GHz with specific absorption rate(SAR) values of 0, 4, and 40 W/kg for 6 min to observe the changes in calcium levels. Results The results showed that the 4 and 40 W/kg microwave radiation caused a significant decrease in the levels of [Ca], [Ca]ER, and [Ca]MIT in primary hippocampal neurons. In the primary cardiomyocytes, only the 40 W/kg microwave radiation caused the decrease in the levels of [Ca], [Ca]ER, and [Ca]MIT. Primary hippocampal neurons were more sensitive to microwave exposure than primary cardiomyocytes. The mitochondria were more sensitive to microwave exposure than the endoplasmic reticulum. Conclusion The calcium efflux was occurred during microwave exposure in primary hippocampal neurons and primary cardiomyocytes. Additionally, neurons and mitochondria were sensitive cells and organelle respectively.

关键词： Real time Microwave Calcium Primary hippocampal neurons Primary cardiomyocytes

Clinicopathological features of 11 cases of chronic hepatitis B infection complicated with primary biliary cholangitis

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World Journal of Hepatology 2023年第4期15卷 577-584页

作者： Yun ye Qian zhang Zhong-hua Lu You-Wen tan Department of Hepatology The Third Hospital of Zhenjiang Affiliated Jiangsu UniversityZhenjiang 212003Jiangsu ProvinceChina Department of Liver Disease Wuxi No.5 People’s Hospital Affiliated to Jiangnan UniversityWuxi 214000Jiangsu ProvinceChina

BACKGROUND Only a few cases of chronic hepatitis B(CHB)with primary biliary cholangitis(PBC)have been reported based on histological evidence from liver *** To observe the clinicopathological features and outcomes of 11 patients with CHB infection complicated by *** Eleven patients with CHB and PBC who underwent liver biopsy at the Zhenjiang Third Hospital,affiliated with Jiangsu University,and Wuxi Fifth People’s Hospital,from January 2005 to September 2020,were *** patients initially visited our hospital with CHB and were pathologically diagnosed with CHB and *** Only five had elevated alkaline phosphatase levels,nine were positive for antimitochondrial antibody(AMA)-M2,and two were negative for *** had jaundice and pruritus symptoms,10 had mildly abnormal liver function,and one had severely elevated bilirubin and liver enzyme *** pathological characteristics of CHB complicated by PBC overlapped with those of PBCautoimmune hepatitis(AIH).When necroinflammation of the portal area is not obvious,the pathological features of PBC are predominant,similar to the features of PBC *** the interface is severe,biliangitis will occur,with a large number of ductular reactions in zone *** the PBC-AIH overlap pathology,this pathology is characterized by a small amount of plasma cell *** PBC,lobulitis is often *** This is the first large case series to show that the rare pathological features of CHB with PBC are similar to those of PBC-AIH and small duct injury was observed.

关键词： Chronic hepatitis B Primary biliary cholangitis Clinicopathological features Antimitochondrial antibody

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一种平面微雕并联机器人系统的设计

微计算机信息 2006年第1Z期22卷 218-220,272页

作者：叶建雄张华谭曼华魏晖南昌大学机电工程学院

根据平面微小并联机器人系统的功能设计要求,在机器人系统的机构、控制分析的基础上,于设计阶段引入正逆向求解,适当的限定条件保证了坐标变换和运动轨迹规划的唯一性,通过软件仿真对整个机构进行了验证,最后完成了整个系统的仿真、设... 详细信息

根据平面微小并联机器人系统的功能设计要求,在机器人系统的机构、控制分析的基础上,于设计阶段引入正逆向求解,适当的限定条件保证了坐标变换和运动轨迹规划的唯一性,通过软件仿真对整个机构进行了验证,最后完成了整个系统的仿真、设计及设备制作。

关键词：微小并联机器人结构分析控制

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一测多评法测定异叶青兰中4种化学成分

世界中医药 2024年第2期19卷 161-165页

作者：张炜谭金华杨凤梅拜礼文肖华李玉林陈涛海平青海省药品检验检测院/青海省中药(藏药)现代化研究重点实验室/国家药品监督管理局藏药质量控制重点实验室西宁810016 青海大学医学院西宁810016 中国科学院西北高原生物研究所西宁810008

目的:采用一测多评法(QAMS)同时测定异叶青兰中4个化学成分含量。方法:采用高效液相色谱法(HPLC)建立绿原酸(CA)、木犀草苷(L1)、迷迭香酸(RA)和木犀草素(L2)的相对校正因子(f_(s/i)),并在不同色谱柱和色谱仪上考察4种成分相对校正因子... 详细信息

目的:采用一测多评法(QAMS)同时测定异叶青兰中4个化学成分含量。方法:采用高效液相色谱法(HPLC)建立绿原酸(CA)、木犀草苷(L1)、迷迭香酸(RA)和木犀草素(L2)的相对校正因子(f_(s/i)),并在不同色谱柱和色谱仪上考察4种成分相对校正因子的稳定性和耐用性,比较QAMS和外标法(ESM)测定结果的差异。结果:4个成分的f_(s/i)分别为f_(CA/RA)=1.148、f_(L1/RA)=1.004、f_(L2/RA)=0.689,f_(s/i)在3种HPLC色谱仪及3个不同品牌C18色谱柱的相对标准偏差(RSD)范围为2.95%~3.61%、在不同柱温(25℃、30℃、35℃)RSD范围为0.90%~1.28%,不同体积流量(0.9 mL/min、1.0 mL/min、1.1 mL/min)下的RSD范围为1.24%~2.21%,表明相对校正因子的耐用性良好。分别采用ESM法和QAMS法对12批异叶青兰药材4个化学成分进行含量测定,结果无显著性差异。结论:使用QAMS法测定异叶青兰中4个化学成分含量准确可靠,能为异叶青兰的质量提高提供参考。

关键词：异叶青兰一测多评法高效液相色谱法绿原酸木犀草苷迷迭香酸木犀草素

Effects of soil moisture and light intensity on ecophysiological characteristics of Amorpha fruticosa seedlings

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Journal of Forestry Research 2013年第2期24卷 293-300页

作者： zhang Xiu-ru tan Xiang-feng WANG Ren-qing XU Nan-nan GUO wei-hua Institute of Ecology and Biodiversity College of Life Sciences Shandong University Clinical College of Tianjin Medical University

We investigated the combined effects of soil moisture and light intensity on the growth, development and ecophysiological characteristics of one-year old Amorpha fruticosa seedlings. Soil moisture and light intensity influenced the ecophysiological characteristics of Amorpha fruticosa seedlings. Soil moisture resulted in the decreases of growth rate, individual size, net photosynthetic rate, transpiration rate, leaf water loss rate （WLR）, and biomass accumulation of plant parts, and led to increased leaf water saturation deficit （WSD）. Under water stress, more photosynthetic products were allocated to root growth. With decreasing light intensity, net photosynthetic rate, transpiration rate, chla/b, water saturation deficit, water use efficiency, water loss rate and biomass accumulation declined, while Chla, Chlb, Chla＋b and carotenoids （Car） increased and more photosynthetic products were allocated to stem and leaf growth. Maximum growth vigor, net photosynthetic rate and total biomass accumulation in Amorpha fruticosa seedlings was recorded at 75 80% soil water-holding capacity and 100% light density in greenhouse environments.

关键词： Amorpha fruticosa ecophysiological characteristics light intensity seedling soil moisture

Presentation of Boerhaave's syndrome as an upper-esophageal perforation associated with a right-sided pleural effusion:A case report

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World Journal of Clinical Cases 2022年第18期10卷 6192-6197页

作者： Ni tan Yin-hua Luo Guang-Cai Li Yi-Lin Chen wei tan Yue-hua Xiang Liang Ge Di Yao Ming-hua zhang Pulmonary and Critical Care Medicine Central Hospital of Tujia and Miao Autonomous PrefectureHubei University of MedicineEnshi Autonomous Prefecture 445000Hubei ProvinceChina Cardiovascular Disease Center Central Hospital of Tujia and Miao Autonomous PrefectureHubei University of MedicineEnshi Autonomous Prefecture 445000Hubei ProvinceChina

BACKGROUND Spontaneous esophageal rupture or Boerhaave’s syndrome is a rare and acute disease with a high incidence of misdiagnosis and ***,we aimed to explore the clinical characteristics,diagnosis,treatment,and prognosis of spontaneous esophageal rupture,and to analyze the causes of misdiagnosis during the treatment of spontaneous esophageal *** SUMMARY The clinical features of the patient with spontaneous esophageal rupture misdiagnosed earlier as pleural effusion were retrospectively analyzed and the reasons for misdiagnosis are discussed based on a current review of the *** patient was admitted to a local hospital due to shortness of breath accompanied by vomiting and abdominal distension for five *** on the computed tomography(CT)scan analysis,clinically,right pleural effusion was ***,the patient was unwilling to undergo right closed thoracic *** patient also had intermittent fevers against infection,and during the course of treatment,he complained of chest pain,following which,he was transferred to our ***-like residue drainage fluid was ***-examination of the chest CT scans suggested the presence of spontaneous perforation in the upper left ***,the patient underwent an urgent esophageal hiatus ***,the patient died of infection and respiratory failure due to progressive dyspnea after *** Spontaneous esophageal rupture is a rare disease associated with high *** patients do not present typical clinical symptoms and the disease progresses *** case report highlights the importance of a dynamic review of chest CT scan,not only for the initial identification of segmental injury but also for prioritizing subsequent treatment ***,we have presented some clues for clinicians to recognize and diagnose spontaneous esophageal rupture at rare sites(upper-esophageal segment)through this case report of spontan

关键词： Spontaneous esophageal rupture Chest computed tomography Upper-esophageal perforation Right-sided pleural effusion Misdiagnosis Case report

热处理对选区激光熔化Ti-6Al-4V合金低周疲劳性能的影响

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稀有金属材料与工程 2024年第9期53卷 2503-2512页

作者：李典陈静谭华姚波张思雨郝志伟林鑫西北工业大学材料学院陕西西安710072 西北有色金属研究院陕西西安710016

为提高选区激光熔化Ti-6Al-4V合金的低周疲劳性能,利用OM、SEM、TEM、室温拉伸及低周疲劳试验研究了热处理对选区激光熔化Ti-6Al-4V合金组织及性能的影响。结果表明,沉积态组织表现为外延生长的β柱状晶及晶内呈一定取向关系的针状马氏... 详细信息

为提高选区激光熔化Ti-6Al-4V合金的低周疲劳性能,利用OM、SEM、TEM、室温拉伸及低周疲劳试验研究了热处理对选区激光熔化Ti-6Al-4V合金组织及性能的影响。结果表明,沉积态组织表现为外延生长的β柱状晶及晶内呈一定取向关系的针状马氏体α′,经退火后α′发生分解转变为网篮编织状的α+β,经循环热处理后板条α发生球化,组织由等轴α、板条α和残余β构成。经热处理后,退火态低周疲劳性能达到锻件的87%以上,循环热处理态达到锻件的90%以上。

关键词：选区激光熔化 Ti-6Al-4V合金热处理低周疲劳

某双链季铵盐消毒液在低温下对物体表面的杀菌效果观察

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中国消毒学杂志 2024年第7期41卷 489-491页

作者：钟秋彤张显勇谭俊杰邱智华何威叶俊豪广州市微生物研究所有限公司广东广州510663

目的观察一种双链季铵盐消毒液在-40℃低温环境中的消毒效果,为低温消毒应用提供参考。方法采用载体定量杀菌试验和现场消毒试验方法,对该双链季铵盐消毒液在低温条件下环境表面消毒效果进行观察。结果该双链季铵盐消毒液原液含量为37 g... 详细信息

目的观察一种双链季铵盐消毒液在-40℃低温环境中的消毒效果,为低温消毒应用提供参考。方法采用载体定量杀菌试验和现场消毒试验方法,对该双链季铵盐消毒液在低温条件下环境表面消毒效果进行观察。结果该双链季铵盐消毒液原液含量为37 g/L,pH值为5.03。该消毒液在-40℃下放置>8 h后无析出,无结晶。在-40℃低温冷库中,原液喷洒作用30 min,对织物样片上大肠埃希菌和金黄色葡萄球菌的杀灭对数值均>3.00;原液按10 mL/m^(3)喷雾作用60 min,对玻璃表面的大肠埃希菌和金黄色葡萄球菌杀灭对数值均>3.00。该消毒液原液对新西兰兔完整皮肤无刺激性,对小鼠急性经口毒性试验为无毒级,对小鼠无致微核作用。结论该双链季铵盐消毒液在-40℃低温下可杀灭环境表面大肠埃希菌和金黄色葡萄球菌,属于实际无毒物质。

关键词：双链季铵盐低温消毒剂物体表面消毒效果